LIVIVO - Search results -

Search results

Result 1 - 10 of total 23

Search options

Article ; Online: Role of macrophages and monocytes in hepatitis C virus infections.

Revie, Dennis / Salahuddin, Syed Zaki

2014 Volume 20, Issue 11, Page(s) 2777–2784

Abstract: A number of studies conducted over many years have shown that hepatitis C virus (HCV) can infect a variety of cell types. In vivo infection of monocytes, macrophages, and dendritic cells by HCV has been frequently shown by a number of researchers. These ... ...

Abstract	A number of studies conducted over many years have shown that hepatitis C virus (HCV) can infect a variety of cell types. In vivo infection of monocytes, macrophages, and dendritic cells by HCV has been frequently shown by a number of researchers. These studies have demonstrated replication of HCV by detecting the presence of both negative genomic strands and a variety of non-structural HCV proteins in infected cells. In addition, analyses of genome sequences have also shown that different cell types can harbor different HCV variants. Investigators have also done preliminary studies of which cellular genes are affected by HCV infection, but there have not yet been a sufficient number of these studies to understand the effects of infection on these cells. Analyses of in vitro HCV replication have shown that monocytes, macrophages and dendritic cells can be infected by HCV from patient sera or plasma. These studies suggest that entry and cellular locations may vary between different cell types. Some studies suggest that macrophages may preferentially allow HCV genotype 1 to replicate, but macrophages do not appear to select particular hypervariable regions. Overall, these studies agree with a model where monocytes and macrophages act as an amplification system, in which these cells are infected and show few cytopathic effects, but continuously produce HCV. This allows them to produce virus over an extended time and allows its spread to other cell types.
MeSH term(s)	Hepatitis C/immunology ; Humans ; Mononuclear Phagocyte System/physiology ; Mononuclear Phagocyte System/virology ; Virus Replication
Language	English
Publishing date	2014-02-10
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2185929-2
ISSN	2219-2840 ; 1007-9327
ISSN (online)	2219-2840
ISSN	1007-9327
DOI	10.3748/wjg.v20.i11.2777
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 6039: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Human cell types important for hepatitis C virus replication in vivo and in vitro: old assertions and current evidence.

Revie, Dennis / Salahuddin, Syed Zaki

Virology journal

2011 Volume 8, Page(s) 346

Abstract: Hepatitis C Virus (HCV) is a single stranded RNA virus which produces negative strand RNA as a replicative intermediate. We analyzed 75 RT-PCR studies that tested for negative strand HCV RNA in liver and other human tissues. 85% of the studies that ... ...

Abstract	Hepatitis C Virus (HCV) is a single stranded RNA virus which produces negative strand RNA as a replicative intermediate. We analyzed 75 RT-PCR studies that tested for negative strand HCV RNA in liver and other human tissues. 85% of the studies that investigated extrahepatic replication of HCV found one or more samples positive for replicative RNA. Studies using in situ hybridization, immunofluorescence, immunohistochemistry, and quasispecies analysis also demonstrated the presence of replicating HCV in various extrahepatic human tissues, and provide evidence that HCV replicates in macrophages, B cells, T cells, and other extrahepatic tissues. We also analyzed both short term and long term in vitro systems used to culture HCV. These systems vary in their purposes and methods, but long term culturing of HCV in B cells, T cells, and other cell types has been used to analyze replication. It is therefore now possible to study HIV-HCV co-infections and HCV replication in vitro.
MeSH term(s)	B-Lymphocytes/virology ; Cell Line ; Hepacivirus/growth & development ; Hepacivirus/isolation & purification ; Hepacivirus/pathogenicity ; Hepatocytes/virology ; Humans ; Liver/virology ; Macrophages/virology ; T-Lymphocytes/virology ; Virus Replication
Language	English
Publishing date	2011-07-11
Publishing country	England
Document type	Journal Article ; Review
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/1743-422X-8-346
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Human cell types important for Hepatitis C Virus replication in vivo and in vitro . Old assertions and current evidence

Salahuddin Syed / Revie Dennis

Virology Journal, Vol 8, Iss 1, p

2011 Volume 346

Abstract: Abstract Hepatitis C Virus (HCV) is a single stranded RNA virus which produces negative strand RNA as a replicative intermediate. We analyzed 75 RT-PCR studies that tested for negative strand HCV RNA in liver and other human tissues. 85% of the studies ... ...

Abstract	Abstract Hepatitis C Virus (HCV) is a single stranded RNA virus which produces negative strand RNA as a replicative intermediate. We analyzed 75 RT-PCR studies that tested for negative strand HCV RNA in liver and other human tissues. 85% of the studies that investigated extrahepatic replication of HCV found one or more samples positive for replicative RNA. Studies using in situ hybridization, immunofluorescence, immunohistochemistry, and quasispecies analysis also demonstrated the presence of replicating HCV in various extrahepatic human tissues, and provide evidence that HCV replicates in macrophages, B cells, T cells, and other extrahepatic tissues. We also analyzed both short term and long term in vitro systems used to culture HCV. These systems vary in their purposes and methods, but long term culturing of HCV in B cells, T cells, and other cell types has been used to analyze replication. It is therefore now possible to study HIV-HCV co-infections and HCV replication in vitro .
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	570
Language	English
Publishing date	2011-07-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages

Revie, Dennis / Alberti, Michael O / Prichard, John G / Kelley, Ann S / Salahuddin, S. Zaki

Virology journal. 2010 Dec., v. 7, no. 1 p.155-155

2010

Abstract: BACKGROUND: Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) ...

Abstract	BACKGROUND: Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) as diagnosed by clinical laboratories. RESULTS: HCV was cultured in vitro using our system. HCV RNA was isolated from patients' blood and from HCV cultured in various cell types for up to three months. The 5'UTR of these isolates were used for comparisons. Results revealed a number of sequence changes as compared to the serum RNA. The HCV RNA produced efficiently by infected macrophages, B-cells, and T-cells had sequences similar to HCV-1, which suggests that selection of the variants was performed at the level of macrophages. Virus with sequences similar to HCV-1 replicated better in macrophages than HCV having a 5'UTR similar to HCV-3. CONCLUSIONS: Although HCV-3 replicates in cell types such as B-cells, T-cells, and macrophages, it may require a different primary cell type for the same purpose. Therefore, in our opinion, HCV-3 does not replicate efficiently in macrophages, and patients infected with HCV-3 may contain a population of HCV-1 in their blood.
Keywords	5' untranslated regions ; B-lymphocytes ; Hepatitis C virus ; T-lymphocytes ; genotype ; human cell lines ; macrophages ; virus replication
Language	English
Dates of publication	2010-12
Size	p. 155.
Publishing place	BioMed Central
Document type	Article ; Online
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/1743-422X-7-155
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages.

Revie, Dennis / Alberti, Michael O / Prichard, John G / Kelley, Ann S / Salahuddin, S Zaki

Virology journal

2010 Volume 7, Page(s) 155

Abstract: Background: Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV- ... ...

Abstract	Background: Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) as diagnosed by clinical laboratories. Results: HCV was cultured in vitro using our system. HCV RNA was isolated from patients' blood and from HCV cultured in various cell types for up to three months. The 5'UTR of these isolates were used for comparisons. Results revealed a number of sequence changes as compared to the serum RNA. The HCV RNA produced efficiently by infected macrophages, B-cells, and T-cells had sequences similar to HCV-1, which suggests that selection of the variants was performed at the level of macrophages. Virus with sequences similar to HCV-1 replicated better in macrophages than HCV having a 5'UTR similar to HCV-3. Conclusions: Although HCV-3 replicates in cell types such as B-cells, T-cells, and macrophages, it may require a different primary cell type for the same purpose. Therefore, in our opinion, HCV-3 does not replicate efficiently in macrophages, and patients infected with HCV-3 may contain a population of HCV-1 in their blood.
MeSH term(s)	5' Untranslated Regions ; B-Lymphocytes/virology ; Base Sequence ; Genotype ; Hepacivirus/classification ; Hepacivirus/genetics ; Hepacivirus/growth & development ; Hepacivirus/isolation & purification ; Hepatitis C/virology ; Humans ; Macrophages/virology ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; T-Lymphocytes/virology ; Virus Replication
Chemical Substances	5' Untranslated Regions
Language	English
Publishing date	2010-07-13
Publishing country	England
Document type	Journal Article
ISSN	1743-422X
ISSN (online)	1743-422X
DOI	10.1186/1743-422X-7-155
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages

Prichard John G / Alberti Michael O / Revie Dennis / Kelley Ann S / Salahuddin S Zaki

Virology Journal, Vol 7, Iss 1, p

2010 Volume 155

Abstract: Abstract Background Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype ... ...

Abstract	Abstract Background Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) as diagnosed by clinical laboratories. Results HCV was cultured in vitro using our system. HCV RNA was isolated from patients' blood and from HCV cultured in various cell types for up to three months. The 5'UTR of these isolates were used for comparisons. Results revealed a number of sequence changes as compared to the serum RNA. The HCV RNA produced efficiently by infected macrophages, B-cells, and T-cells had sequences similar to HCV-1, which suggests that selection of the variants was performed at the level of macrophages. Virus with sequences similar to HCV-1 replicated better in macrophages than HCV having a 5'UTR similar to HCV-3. Conclusions Although HCV-3 replicates in cell types such as B-cells, T-cells, and macrophages, it may require a different primary cell type for the same purpose. Therefore, in our opinion, HCV-3 does not replicate efficiently in macrophages, and patients infected with HCV-3 may contain a population of HCV-1 in their blood.
Keywords	Microbiology ; QR1-502 ; Science ; Q ; DOAJ:Microbiology ; DOAJ:Biology ; DOAJ:Biology and Life Sciences ; Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	360
Language	English
Publishing date	2010-07-01T00:00:00Z
Publisher	BioMed Central
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Discovery of significant variants containing large deletions in the 5'UTR of human hepatitis C virus (HCV).

Revie, Dennis / Alberti, Michael O / Braich, Ravi S / Bayles, David / Prichard, John G / Salahuddin, S Zaki

Virology journal

2006 Volume 3, Page(s) 82

Abstract: We recently reported the isolation and in vitro replication of hepatitis C virus. These isolates were termed CIMM-HCV and analyzed to establish genotypes and subtypes, which are reported elsewhere. During this analysis, an HCV isolated from a patient was ...

Abstract	We recently reported the isolation and in vitro replication of hepatitis C virus. These isolates were termed CIMM-HCV and analyzed to establish genotypes and subtypes, which are reported elsewhere. During this analysis, an HCV isolated from a patient was discovered that had large deletions in the 5'UTR. 57% of the HCV RNA found in this patient's sera had 113 or 116 bp deletions. Sequence data showed that domains IIIa to IIIc were missing. Previous studies have suggested that these domains may be important for translation. In vitro replicated HCV from this patient did not contain these deletions, however, it contained a 148 bp deletion in the 5'UTR. Whereas the patient HCV lacked domains IIIa through IIIc, the isolate lacked domains IIIa through IIId. HCV from this patient continues to produce large deletions in vitro, suggesting that the deletion may not be important for the assembly or replication of the virus. This is the first report describing these large deletions.
MeSH term(s)	5' Untranslated Regions/genetics ; Female ; Gene Deletion ; Genetic Variation ; Hepacivirus/genetics ; Hepacivirus/isolation & purification ; Hepatitis C, Chronic/virology ; Humans ; Middle Aged ; Molecular Sequence Data
Chemical Substances	5' Untranslated Regions
Language	English
Publishing date	2006-09-29
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	2160640-7
ISSN	1743-422X ; 1743-422X
ISSN (online)	1743-422X
ISSN	1743-422X
DOI	10.1186/1743-422X-3-82
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Discovery of significant variants containing large deletions in the 5'UTR of human hepatitis C virus (HCV)

Revie, Dennis / Alberti, Michael O / Braich, Ravi S / Bayles, David / Prichard, John G / Salahuddin, S Zaki

Virology journal. 2006 Dec., v. 3, no. 1

2006

Abstract	We recently reported the isolation and in vitro replication of hepatitis C virus. These isolates were termed CIMM-HCV and analyzed to establish genotypes and subtypes, which are reported elsewhere. During this analysis, an HCV isolated from a patient was discovered that had large deletions in the 5'UTR. 57% of the HCV RNA found in this patient's sera had 113 or 116 bp deletions. Sequence data showed that domains IIIa to IIIc were missing. Previous studies have suggested that these domains may be important for translation. In vitro replicated HCV from this patient did not contain these deletions, however, it contained a 148 bp deletion in the 5'UTR. Whereas the patient HCV lacked domains IIIa through IIIc, the isolate lacked domains IIIa through IIId. HCV from this patient continues to produce large deletions in vitro, suggesting that the deletion may not be important for the assembly or replication of the virus. This is the first report describing these large deletions.
Keywords	5' untranslated regions ; Hepatitis C virus ; humans ; patients ; virology ; viruses
Language	English
Dates of publication	2006-12
Size	p. 82.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/1743-422X-3-82
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Analysis of in vitro replicated human hepatitis C virus (HCV) for the determination of genotypes and quasispecies

Revie, Dennis / Alberti, Michael O / Braich, Ravi S / Chelyapov, Nickolas / Bayles, David / Prichard, John G / Salahuddin, S Zaki

Virology journal. 2006 Dec., v. 3, no. 1

2006

Abstract: Isolation and self-replication of infectious HCV has been a difficult task. However, this is needed for the purposes of developing rational drugs and for the analysis of the natural virus. Our recent report of an in vitro system for the isolation of ... ...

Abstract	Isolation and self-replication of infectious HCV has been a difficult task. However, this is needed for the purposes of developing rational drugs and for the analysis of the natural virus. Our recent report of an in vitro system for the isolation of human HCV from infected patients and their replication in tissue culture addresses this challenge. At California Institute of Molecular Medicine several isolates of HCV, called CIMM-HCV, were grown for over three years in cell culture. This is a report of the analysis of CIMM-HCV isolates for subtypes and quasispecies using a 269 bp segment of the 5'UTR. HCV RNA from three patients and eleven CIMM-HCV were analyzed for this purpose. All isolates were essentially identical. Isolates of HCV from one patient were serially transmitted into fresh cells up to eight times and the progeny viruses from each transmission were compared to each other and also to the primary isolates from the patient's serum. Some isolates were also transmitted to different cell types, while others were cultured continuously without retransmission for over three years. We noted minor sequence changes when HCV was cultured for extended periods of time. HCV in T-cells and non-committed lymphoid cells showed a few differences when compared to isolates obtained from immortalized B-cells. These viruses maintained close similarity despite repeated transmissions and passage of time. There were no subtypes or quasispecies noted in CIMM-HCV.
Keywords	5' untranslated regions ; Hepatitis C virus ; T-lymphocytes ; blood serum ; cell culture ; humans ; medicine ; patients ; progeny ; tissue culture ; virology ; viruses ; California
Language	English
Dates of publication	2006-12
Size	p. 81.
Publishing place	BioMed Central
Document type	Article
ZDB-ID	2160640-7
ISSN	1743-422X
ISSN	1743-422X
DOI	10.1186/1743-422X-3-81
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Student Attitudes Contribute to the Effectiveness of a Genomics CURE.

Lopatto, David / Rosenwald, Anne G / Burgess, Rebecca C / Silver Key, Catherine / Van Stry, Melanie / Wawersik, Matthew / DiAngelo, Justin R / Hark, Amy T / Skerritt, Matthew / Allen, Anna K / Alvarez, Consuelo / Anderson, Sara / Arrigo, Cindy / Arsham, Andrew / Barnard, Daron / Bedard, James E J / Bose, Indrani / Braverman, John M / Burg, Martin G /

Croonquist, Paula / Du, Chunguang / Dubowsky, Sondra / Eisler, Heather / Escobar, Matthew A / Foulk, Michael / Giarla, Thomas / Glaser, Rivka L / Goodman, Anya L / Gosser, Yuying / Haberman, Adam / Hauser, Charles / Hays, Shan / Howell, Carina E / Jemc, Jennifer / Jones, Christopher J / Kadlec, Lisa / Kagey, Jacob D / Keller, Kimberly L / Kennell, Jennifer / Kleinschmit, Adam J / Kleinschmit, Melissa / Kokan, Nighat P / Kopp, Olga Ruiz / Laakso, Meg M / Leatherman, Judith / Long, Lindsey J / Manier, Mollie / Martinez-Cruzado, Juan C / Matos, Luis F / McClellan, Amie Jo / McNeil, Gerard / Merkhofer, Evan / Mingo, Vida / Mistry, Hemlata / Mitchell, Elizabeth / Mortimer, Nathan T / Myka, Jennifer Leigh / Nagengast, Alexis / Overvoorde, Paul / Paetkau, Don / Paliulis, Leocadia / Parrish, Susan / Toering Peters, Stephanie / Preuss, Mary Lai / Price, James V / Pullen, Nicholas A / Reinke, Catherine / Revie, Dennis / Robic, Srebrenka / Roecklein-Canfield, Jennifer A / Rubin, Michael R / Sadikot, Takrima / Sanford, Jamie Siders / Santisteban, Maria / Saville, Kenneth / Schroeder, Stephanie / Shaffer, Christopher D / Sharif, Karim A / Sklensky, Diane E / Small, Chiyedza / Smith, Sheryl / Spokony, Rebecca / Sreenivasan, Aparna / Stamm, Joyce / Sterne-Marr, Rachel / Teeter, Katherine C / Thackeray, Justin / Thompson, Jeffrey S / Velazquez-Ulloa, Norma / Wolfe, Cindy / Youngblom, James / Yowler, Brian / Zhou, Leming / Brennan, Janie / Buhler, Jeremy / Leung, Wilson / Elgin, Sarah C R / Reed, Laura K

Journal of microbiology & biology education

2022 Volume 23, Issue 2

Abstract: The Genomics Education Partnership (GEP) engages students in a course-based undergraduate research experience (CURE). To better understand the student attributes that support success in this CURE, we asked students about their attitudes using previously ... ...

Abstract	The Genomics Education Partnership (GEP) engages students in a course-based undergraduate research experience (CURE). To better understand the student attributes that support success in this CURE, we asked students about their attitudes using previously published scales that measure epistemic beliefs about work and science, interest in science, and grit. We found, in general, that the attitudes students bring with them into the classroom contribute to two outcome measures, namely, learning as assessed by a pre- and postquiz and perceived self-reported benefits. While the GEP CURE produces positive outcomes overall, the students with more positive attitudes toward science, particularly with respect to epistemic beliefs, showed greater gains. The findings indicate the importance of a student's epistemic beliefs to achieving positive learning outcomes.
Language	English
Publishing date	2022-05-16
Publishing country	United States
Document type	Journal Article
ISSN	1935-7877
ISSN	1935-7877
DOI	10.1128/jmbe.00208-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED