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Article ; Online: [Epstein-Barr virus (Herpesviridae:

Smirnova, K V / Lubenskaya, A K / Senyuta, N B / Dushenkina, T E / Gurtsevitch, V E

2023 Volume 68, Issue 4, Page(s) 291–301

Abstract: Introduction: The discovery of two types of Epstein-Barr virus (EBV) (EBV-1 and EBV-2) that have different biological properties stimulated the search for neoplasms associated with each type of the virus. The aim of the work is to study the nature of ... ...

Abstract	Introduction: The discovery of two types of Epstein-Barr virus (EBV) (EBV-1 and EBV-2) that have different biological properties stimulated the search for neoplasms associated with each type of the virus. The aim of the work is to study the nature of the association of nasopharyngeal cancer (NPC) with EBV-1 and EBV-2, serological activity for each viral type and the concentration of EBV DNA in the blood plasma of two gender, age and ethnic groups of NPC patients that represent geographically and climatically different regions of Russia,. Materials and methods: In the blood plasma of patients with NPC and other non- EBV associated tumors of oral cavity (OTOC Results: The blood plasma samples testing showed that NPC and OTOC Conclusion: The lack of the predominance of one of EBV types in NPC patients that are the representatives of different ethnic groups from geographically and climatically different regions, suggests that none of these factors play an important role in the NPC carcinogenesis. Evidently, each type of EBV, EBV-1 or EBV-2, if the necessary conditions arise, are able to exhibit its oncogenic potential to initiate tumor development.
MeSH term(s)	Humans ; Herpesvirus 4, Human/genetics ; Nasopharyngeal Carcinoma/epidemiology ; Nasopharyngeal Neoplasms/epidemiology ; Epstein-Barr Virus Infections ; Lymphocryptovirus/genetics ; DNA, Viral/genetics ; Biomarkers ; Antigens, Viral/genetics ; Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G
Chemical Substances	DNA, Viral ; Biomarkers ; Antigens, Viral ; Antibodies, Viral ; Immunoglobulin A ; Immunoglobulin G
Language	Russian
Publishing date	2023-09-21
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article
ZDB-ID	201241-8
ISSN	2411-2097 ; 0507-4088
ISSN (online)	2411-2097
ISSN	0507-4088
DOI	10.36233/0507-4088-181
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Article ; Online: [Epstein-Barr virus (Herpesviridae: Gammaherpesvirinae: Lymphocryptovirus: Human gammaherpesvirus 4) in Kalmyks and Slavs living in Russia: virus types, LMP1 oncogene variants, and malignancies].

Gurtsevitch, V E / Lubenskaya, A K / Senyuta, N B / Dushenkina, T E / Smirnova, K V

Voprosy virusologii

2022 Volume 67, Issue 3, Page(s) 246–257

Abstract: Introduction: The discovery of the Epstein-Barr virus types (Herpesviridae: Gammaherpesvirinae: Lymphocryptovirus: Human gammaherpesvirus 4) (EBV) - EBV-1 and EBV-2, which have different transforming abilities in vitro, stimulated the study of their ... ...

Abstract	Introduction: The discovery of the Epstein-Barr virus types (Herpesviridae: Gammaherpesvirinae: Lymphocryptovirus: Human gammaherpesvirus 4) (EBV) - EBV-1 and EBV-2, which have different transforming abilities in vitro, stimulated the study of their prevalence in populations in order to elucidate the relationship with malignant neoplasms.The aims of the work are to study the prevalence of EBV-1 and EBV-2 among representatives of 2 ethnic groups of Russia, Kalmyks and Slavs, sequencing analysis of the LMP1 oncogene in virus isolates, and analysis of the correlation between virus types and the incidence of certain forms of tumors. Materials and methods: DNA samples were isolated from the biological material of oral swabs obtained from ethnic Kalmyks of the Republic of Kalmykia (RK) (n = 50) and Slavs, residents of the Moscow Region (MR) (n = 40). DNA samples were used to amplify EBV DNA, followed by determination of its concentration per 1 cell of washout, amplification of the LMP1 oncogene in viral samples, their sequencing, and determination of LMP1 protein variants. Results: It has been established that with the same burden of EBV among representatives of both ethnic groups in the Kalmyk group, the ratio of persons infected with transforming and non-transforming types of the virus was almost the same (EBV-1 - 51%; and EBV-2 - 49%). Meanwhile, in the group of Slavs the transforming EBV-1 type virus dominated (80.6%). The predominance of EBV-1 type in representatives of the Slavs correlated with increased incidence of certain forms of tumors in the population of the MR when compared with similar values in the population of the RK, where both types of the virus were prevalent. Differences between the compared rates of cancer incidence were not statistically significant. Analysis of viral isolates showed a similar set of LMP1 variants in both ethnic groups. Conclusion: In order to establish the influence of EBV types on the incidence of malignant tumors, additional studies involving representatives of various ethnic groups from different geographical regions are needed.
MeSH term(s)	Epstein-Barr Virus Infections/epidemiology ; Epstein-Barr Virus Infections/genetics ; Epstein-Barr Virus Infections/pathology ; Gammaherpesvirinae/genetics ; Herpesvirus 4, Human/metabolism ; Humans ; Lymphocryptovirus/genetics ; Neoplasms ; Oncogenes ; Russia/epidemiology ; Viral Matrix Proteins/genetics
Chemical Substances	Viral Matrix Proteins
Language	Russian
Publishing date	2022-07-14
Publishing country	Russia (Federation)
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	201241-8
ISSN	2411-2097 ; 0507-4088
ISSN (online)	2411-2097
ISSN	0507-4088
DOI	10.36233/0507-4088-120
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Article: Human oncogenic viruses: hepatitis B and hepatitis C viruses and their role in hepatocarcinogenesis.

Gurtsevitch, V E

Biochemistry. Biokhimiia

2008 Volume 73, Issue 5, Page(s) 504–513

Abstract: ... of these viral infections result from the interaction between these viruses and host hepatocytes (i.e. between viral and ...

Abstract	Chronic infections caused by hepatitis B virus (HBV) and/or hepatitis C virus (HCV) are the main risk factors for the development of hepatocellular carcinoma (HCC) in humans. Both viruses cause a wide spectrum of clinical manifestations ranging from healthy carrier state to acute and chronic hepatitis, liver cirrhosis, and HCC. HBV and HCV belong to different viral families (Hepadnoviridae and Flaviviridae, respectively); they are characterized by different genetic structures. Clinical manifestations of these viral infections result from the interaction between these viruses and host hepatocytes (i.e. between viral and cell genomes). Proteins encoded by both viruses play an important role in processes responsible for immortalization and transformation of these cells. Chronic inflammation determined by host immune response to the viral infection, hepatocyte death and their compensatory proliferation, as well as modulation of expression of some regulatory proteins of the cell (growth factors, cytokines, etc.) are the processes that play the major role in liver cancer induced by HBV and HCV.
MeSH term(s)	Animals ; Carcinoma, Hepatocellular/genetics ; Carcinoma, Hepatocellular/immunology ; Carcinoma, Hepatocellular/pathology ; Carcinoma, Hepatocellular/virology ; Hepacivirus/genetics ; Hepacivirus/immunology ; Hepacivirus/metabolism ; Hepacivirus/pathogenicity ; Hepatitis B virus/genetics ; Hepatitis B virus/immunology ; Hepatitis B virus/metabolism ; Hepatitis B virus/pathogenicity ; Humans ; Oncogenic Viruses/genetics ; Oncogenic Viruses/immunology ; Oncogenic Viruses/metabolism ; Oncogenic Viruses/pathogenicity ; Stress, Physiological/immunology ; Stress, Physiological/physiopathology ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism
Chemical Substances	Tumor Suppressor Protein p53
Language	English
Publishing date	2008-06-11
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1109-5
ISSN	1608-3040 ; 0006-2979 ; 0320-9717
ISSN (online)	1608-3040
ISSN	0006-2979 ; 0320-9717
DOI	10.1134/s0006297908050039
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Article: [Molecular biological properties of the Epstein-Barr virus LMP1 gene: structure, function and polymorphism].

Smirnova, K V / Diduk, S V / Senyuta, N B / Gurtsevitch, V E

Voprosy virusologii

2015 Volume 60, Issue 3, Page(s) 5–13

Abstract: Recent studies indicate that the latent membrane protein 1 (LMP1) encoded by the same name gene of the Epstein-Barr virus (EBV) plays an extremely important role in the pathogenesis of a number of malignant neoplasia. Specifically, LMP1 has the ability ... ...

Abstract	Recent studies indicate that the latent membrane protein 1 (LMP1) encoded by the same name gene of the Epstein-Barr virus (EBV) plays an extremely important role in the pathogenesis of a number of malignant neoplasia. Specifically, LMP1 has the ability to transform human B-lymphocytes in vivo and in vitro and rodent fibroblasts (Rat-1) in vitro. The introduction of the latter into athymic mice leads to tumor development. In addition, expression of the oncoprotein has been often found in EBV-associated tumors at the DNA and constantly at the RNA levels. Having pleiotropic effects, LMP1, participates in the transmission and activation of multiple intracellular signals. It is also involved in the inhibition of key tumor suppressors, has significant influence on proliferation, apoptosis and morphological alteration of the infected cells finally resulting in their transformation. General characteristics of EBV and LMP1 gene as well as functional activity of the encoded LMP1 protein and a brief description of human pathologies associated with the virus have been discussed in this review. The questions concerning the polymorphism LMP1 in EBV-associated pathologies have been also analyzed in details.
MeSH term(s)	Animals ; B-Lymphocytes/immunology ; B-Lymphocytes/pathology ; B-Lymphocytes/virology ; Burkitt Lymphoma/genetics ; Burkitt Lymphoma/immunology ; Burkitt Lymphoma/pathology ; Burkitt Lymphoma/virology ; Cell Transformation, Neoplastic/genetics ; Cell Transformation, Neoplastic/immunology ; Cell Transformation, Neoplastic/pathology ; Epstein-Barr Virus Infections/genetics ; Epstein-Barr Virus Infections/immunology ; Epstein-Barr Virus Infections/pathology ; Epstein-Barr Virus Infections/virology ; Gene Expression Regulation, Viral ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/immunology ; Herpesvirus 4, Human/pathogenicity ; Host-Pathogen Interactions ; Humans ; Mice ; Mice, Nude ; Polymorphism, Genetic ; Rats ; Signal Transduction ; Viral Matrix Proteins/chemistry ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/immunology ; Virus Latency
Chemical Substances	EBV-associated membrane antigen, Epstein-Barr virus ; Viral Matrix Proteins
Language	Russian
Publishing date	2015
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	201241-8
ISSN	0507-4088
ISSN	0507-4088
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Article: STRUCTURAL AND FUNCTIONAL CHARACT ERISTICS OF THE LMP1 ONCOGENE IN PATIENTS WITH TUMORS ASSOCIATED AND NOT ASSOCIATED WITH THE EPSTEIN-BARR VIRUS.

Senyuta, N B / Smirnova, K V / Diduk, S V / Goncharova, E V / Shcherbak, L N / Gurtsevitch, V E

Molekuliarnaia genetika, mikrobiologiia i virusologiia

2018 Volume 34, Issue 2, Page(s) 71–75

Abstract: Epstein-Barr virus (EBV) - the etiological agent of a number of human benign and malignant tumors including infectious mononucleosis (IM), Burkitt lymphoma (BL), Hodgkin (HL) and non-Hodgkin (NLH) lymphomas, nasopharyngeal carcinoma (NPC), and many other ...

Abstract	Epstein-Barr virus (EBV) - the etiological agent of a number of human benign and malignant tumors including infectious mononucleosis (IM), Burkitt lymphoma (BL), Hodgkin (HL) and non-Hodgkin (NLH) lymphomas, nasopharyngeal carcinoma (NPC), and many other tumors. Latent membrane protein 1 (LMPl) encoded by the gene of the same name (LMP I) is the main oncoprotein of EBV. LMP1 is a transmembrane protein capable of activating many signaling pathways and transcription factors of the cells, which leads to its transformation. Molecular analysis of LMP1 of various clinical origins identified many variants with different types of amino acid mutations that influence its biological activity. Since the role of LMPl in the development of NPC is still not fully understood, it is important to find out how LMPl samples from patients with EBV-associated form of NPC differ from those of patients with other tumors also located in the oral cavity (OTOC), but not associated with this virus. Unlike most investigations conducted in endemic regions, the present work is intended to compare the genetic structure and the transforming activity of LMPl variants from NPC and OTOC patients has been carried out in a non-endemic region of Russia, where NPC is rarely diagnosed. The obtained data show structural and functional similarities of LMP1 variants in the two groups of patients and, accordingly, a genetic relationship of EBV strains persisting in these patients. Our work suggests that in non-endemic regions any EBV strain with any structure of LMP1 may become the etiologic agent of NPC. However, based on modem concepts, the cancer can occur only if EBV-infected persons have a unique pattern of HLA associated with a high sensitivity to the development of NPC combined with exposure to harmful environmental factors (chemical or physical carcinogens) and lifestyle.
MeSH term(s)	Epstein-Barr Virus Infections/genetics ; Epstein-Barr Virus Infections/metabolism ; Epstein-Barr Virus Infections/pathology ; Female ; Genetic Variation ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/metabolism ; Humans ; Male ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Neoplasms/virology ; Oncogene Proteins/genetics ; Oncogene Proteins/metabolism ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/metabolism
Chemical Substances	EBV-associated membrane antigen, Epstein-Barr virus ; Oncogene Proteins ; Viral Matrix Proteins
Language	Russian
Publishing date	2018-10-31
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	989858-x
ISSN	0208-0613
ISSN	0208-0613
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Article: Diagnostic value of the Epstein-Barr virus serological markers in patients with nasopharyngeal carcinoma in cases of undetectable primary tumor location.

Gurtsevitch, Vladimir E / Senyuta, N B / Lomaya, M V / Ignatova, A V / Dushenkina, T E / Repkina, I A / Pavlovskaya, A I / Mudunov, A M

Voprosy virusologii

2018 Volume 61, Issue 5, Page(s) 205–212

Abstract: The goal of this work was to describe a method for diagnosis of the non-keratinizing nasopharyngeal carcinoma (nNPC) in cases of the undetectable primary tumor location. The method is based on evaluation of IgG and IgA antibody levels to the capsid (VCA) ...

Abstract	The goal of this work was to describe a method for diagnosis of the non-keratinizing nasopharyngeal carcinoma (nNPC) in cases of the undetectable primary tumor location. The method is based on evaluation of IgG and IgA antibody levels to the capsid (VCA) and early antigens (EA) of the Epstein-Barr virus (EBV). The diagnosis of nNPC is established by a so-called decision rule. The latter was created by mathematical processing of the method of multifactor analysis of the results of anti-EBV antibody testing of 72 patients with clinically and morphologically confirmed nNPC and 72 patients with other head and neck benign tumors (OHNT) not associated with EBV, which were tested as a control group. The diagnostic value of the decision rule which was tested in the group of 77 patients with confirmed nNPC and 231 patients of a control group was high. The numbers of false negative and false positive cases were equal to 5.2% (4/77) and 6.5% (17/231), respectively. Among 32 patients with undetectable primary tumors the decision rule was able to identify 11 cases of nNPC. This diagnosis later was confirmed by morphological and instrumental methods of study. Only in two cases, false negative result was obtained (2/32; 6.3%) indicating that the serological diagnostics of nNPC with the decision rule is highly specific but not exact. Thus, the data obtained allowed us to conclude that the serological testing of EBV specific antibody evaluated by the decision rule can be recommended as an important test supplementing the standard methods of pdNPC diagnostics including cases with undetected primary tumor location.
MeSH term(s)	Adult ; Aged ; Antibodies, Viral/blood ; Antigens, Viral/blood ; Antigens, Viral/immunology ; Biomarkers/blood ; Capsid Proteins/blood ; Capsid Proteins/immunology ; Carcinoma/complications ; Carcinoma/diagnosis ; Carcinoma/immunology ; Carcinoma/virology ; Case-Control Studies ; Clinical Decision-Making/methods ; Epstein-Barr Virus Infections/complications ; Epstein-Barr Virus Infections/diagnosis ; Epstein-Barr Virus Infections/immunology ; Epstein-Barr Virus Infections/virology ; Factor Analysis, Statistical ; False Negative Reactions ; False Positive Reactions ; Female ; Head and Neck Neoplasms/complications ; Head and Neck Neoplasms/diagnosis ; Head and Neck Neoplasms/immunology ; Head and Neck Neoplasms/virology ; Herpesvirus 4, Human/immunology ; Herpesvirus 4, Human/isolation & purification ; Humans ; Immunoglobulin A/blood ; Immunoglobulin G/blood ; Male ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms/complications ; Nasopharyngeal Neoplasms/diagnosis ; Nasopharyngeal Neoplasms/immunology ; Nasopharyngeal Neoplasms/virology ; Neoplasms/complications ; Neoplasms/diagnosis ; Neoplasms/immunology ; Neoplasms/virology
Chemical Substances	Antibodies, Viral ; Antigens, Viral ; Biomarkers ; Capsid Proteins ; Immunoglobulin A ; Immunoglobulin G
Language	English
Publishing date	2018-01-11
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	201241-8
ISSN	0507-4088
ISSN	0507-4088
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Article ; Online: EPSTEIN-BARR VIRUS AND NASOPHARYNGEAL CARCINOMA: VIRAL MARKERS FOR DIAGNOSTICS AND ASSESSMENT OF CLINICAL STATUS OF PATIENTS.

Kondratova, V N / Lomaya, M V / Ignatova, A V / Dushenkina, T E / Smirnova, K V / Mudunov, A M / Lichtenstein, A V / Gurtsevitch, V E / Senyuta, N B

Voprosy virusologii

2018 Volume 63, Issue 2, Page(s) 77–84

Abstract: The etiological role of the Epstein-Barr virus (EBV) in the development of an undifferentiated histological variant of nasopharyngeal carcinoma (uNPC) found for the first time in regions with a high incidence of this pathology, the Southern provinces of ... ...

Abstract	The etiological role of the Epstein-Barr virus (EBV) in the development of an undifferentiated histological variant of nasopharyngeal carcinoma (uNPC) found for the first time in regions with a high incidence of this pathology, the Southern provinces of China and the countries of Southeast Asia, and later in the rest of the world, has served as a basis for the widespread use of EBV serological markers for the diagnosis of this form of tumor. In recent years, the use of a test based on the quantitative determination of the EBV DNA concentration in the blood plasma of uNPC patients for early detection and monitoring of the disease has become widespread in endemic regions. In non-endemic regions, such studies virtually have not been carried out, and moreover, the comparative evaluation of the significance of two viral markers, serological and EBV DNA load in the bloodstream of uNPC patients, for diagnostics and evaluation of the therapeutic effect was not investigated. The aim of this study was to compare the clinical value of two serological markers and plasma EBV DNA load in uNPC patients from non-endemic region (Russia). The obtained results indicate that IgA antibodies to the viral capsid antigen (IgA/VCA) and plasma EBV DNA concentration can be successfully used for the diagnosis of uNPC, while IgG/VCA antibodies have no practical significance as an uNPC marker. In addition, it was found that plasma EBV DNA load is more sensitive marker of uNPC than IgA/VCA titers because DNA copy numbers reflect more accurately the effect of the therapy and the clinical state of patients at the stages of remission or relapse. It was shown for the first time that in the non-endemic region the simultaneous evaluation of IgA/VCA antibody levels and the plasma EBV DNA loads are the most effective markers for the diagnostics of uNPC. However, we believe, that it is more practical to use IgA/VCA antibody levels for uNPC screening, and plasma EBV DNA copies - for monitoring of the disease.
Language	English
Publishing date	2018-04-20
Publishing country	Russia (Federation)
Document type	Journal Article
ZDB-ID	201241-8
ISSN	2411-2097 ; 0507-4088
ISSN (online)	2411-2097
ISSN	0507-4088
DOI	10.18821/0507-4088-2018-63-2-77-84
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Article: [Epstein-Barr Virus LMP1 oncogene variants in cell lines of different origin].

Yakovleva, L S / Senyuta, N B / Goncharova, E V / Scherback, L N / Smirnova, R V / Pavlish, O A / Gurtsevitch, V E

Molekuliarnaia biologiia

2015 Volume 49, Issue 5, Page(s) 800–810

Abstract: It is well known that the Epstein-Barr virus (EBV) is a widespread infection in the human population. Typically, infection occurs in early childhood without serious consequences for infected people. At the same time, a secondary infection with an ... ...

Abstract	It is well known that the Epstein-Barr virus (EBV) is a widespread infection in the human population. Typically, infection occurs in early childhood without serious consequences for infected people. At the same time, a secondary infection with an additional EBV strain occurs quite often. During the in vitro cultivation of peripheral blood lymphocytes from persons infected with multiple strains of the virus, only one of these strains with higher transforming potential becomes dominant, while the others are eliminated. Under certain conditions, such a highly transforming EBV strain apparently is able to be the etiologic agent of EBVassociated diseases. To find out the range of highly transforming EBV strains prevalent among Russians, cell lines from patients with EBV-associated and non-associated tumors, as well as healthy individuals, were established. The structural analysis of the latent membrane protein 1 gene (LMP1), a key oncogene of the virus, isolated from established cell lines and peripheral blood lymphocytes of blood donors was carried out, and data obtained were compared with the respective data for LMP1 isolates, amplified from cell lines established from African and Japanese patients with Burkitt's lymphoma. The data obtained show a genetic relationship between Russian LMP1 isolates regardless the fact whether they come from patients with tumors or healthy individuals and differ significantly from LMP1 isolates from Burkitt's lymphoma patients. Thus, the results of the study suggest that in nonendemic region for EBV-associated pathology, Russia, any strain of EBV with any structure of LMP1 with concomitant effect of additional factors may become an etiologic agent for EBV-associated neoplasia.
MeSH term(s)	Adult ; Africa/epidemiology ; B-Lymphocytes/metabolism ; B-Lymphocytes/pathology ; B-Lymphocytes/virology ; Cell Line, Tumor ; Child ; Epstein-Barr Virus Infections/epidemiology ; Epstein-Barr Virus Infections/genetics ; Epstein-Barr Virus Infections/pathology ; Epstein-Barr Virus Infections/virology ; Gene Expression Regulation, Viral ; Herpesvirus 4, Human/classification ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/pathogenicity ; Host-Pathogen Interactions ; Humans ; Japan/epidemiology ; Molecular Sequence Data ; Oncogene Proteins, Viral/chemistry ; Oncogene Proteins, Viral/genetics ; Oncogene Proteins, Viral/metabolism ; Phylogeny ; Polymorphism, Genetic ; Repetitive Sequences, Amino Acid ; Russia/epidemiology ; Viral Matrix Proteins/chemistry ; Viral Matrix Proteins/genetics ; Viral Matrix Proteins/metabolism
Chemical Substances	EBV-associated membrane antigen, Epstein-Barr virus ; Oncogene Proteins, Viral ; Viral Matrix Proteins
Language	Russian
Publishing date	2015-09
Publishing country	Russia (Federation)
Document type	English Abstract ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	213542-5
ISSN	0026-8984
ISSN	0026-8984
DOI	10.7868/S0026898415050213
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Article ; Online: Epstein-Barr virus biomarkers for nasopharyngeal carcinoma in non-endemic regions.

Gurtsevitch, V E / Senyuta, N B / Ignatova, A V / Lomaya, M V / Kondratova, V N / Pavlovskaya, A I / Dushenkina, T E / Maximovich, D M / Smirnova, K V / Mudunov, A M / Lichtenstein, A V

The Journal of general virology

2017 Volume 98, Issue 8, Page(s) 2118–2127

Abstract: The Epstein-Barr virus (EBV) plays a key role in the development of undifferentiated nasopharyngeal carcinoma (uNPC). In uNPC endemic regions EBV-specific antibodies and plasma EBV DNA load are used as markers for the early detection of uNPC and ... ...

Abstract	The Epstein-Barr virus (EBV) plays a key role in the development of undifferentiated nasopharyngeal carcinoma (uNPC). In uNPC endemic regions EBV-specific antibodies and plasma EBV DNA load are used as markers for the early detection of uNPC and monitoring of the disease. In non-endemic regions, such studies were practically not conducted. The aim of this study was to compare the clinical significance of EBV serological markers and plasma EBV DNA levels for uNPC patients in a non-endemic region, Russia. The results obtained indicate that both viral capsid antigen/immunoglobulin A (VCA/IgA) antibodies and plasma EBV DNA copies can effectively be used for nasopharyngeal carcinoma (NPC) diagnosis. Besides, plasma EBV DNA load was found to be a more sensitive marker of uNPC than VCA/IgA antibody titres, as it reflected the effect of the therapy in stages of remission and relapse of the disease more precisely. Our study, for the first time, demonstrates that the simultaneous use of plasma EBV DNA loads and VCA/IgA antibody levels are indispensable markers for uNPC in non-endemic regions: a serological marker can be more effectively used for NPC screening, but EBV DNA copies are better for monitoring the disease. However, both markers turned out to be practically unsuitable for assessing the clinical status of patients. Serological markers did not correlate with any signs of the tumour process estimated by tumour, node and metastasis (TNM) classification and the plasma EBV DNA loads correlated only with the size of the pathologically altered lymph nodes (N). Additional study is required to confirm these findings.
MeSH term(s)	Adult ; Antibodies, Viral/blood ; Capsid Proteins/genetics ; Capsid Proteins/metabolism ; Carcinoma/blood ; Carcinoma/virology ; DNA, Viral/genetics ; DNA, Viral/metabolism ; Epstein-Barr Virus Infections/blood ; Epstein-Barr Virus Infections/virology ; Female ; Herpesvirus 4, Human/classification ; Herpesvirus 4, Human/genetics ; Herpesvirus 4, Human/immunology ; Herpesvirus 4, Human/isolation & purification ; Humans ; Male ; Middle Aged ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms/blood ; Nasopharyngeal Neoplasms/virology ; Russia
Chemical Substances	Antibodies, Viral ; Capsid Proteins ; DNA, Viral
Language	English
Publishing date	2017-08-08
Publishing country	England
Document type	Journal Article
ZDB-ID	219316-4
ISSN	1465-2099 ; 0022-1317
ISSN (online)	1465-2099
ISSN	0022-1317
DOI	10.1099/jgv.0.000889
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Article ; Online: P20

V. Gurtsevitch / L. Yakovleva / L. Scherback / E. Goncharova / K. Smirnova / N. Senyuta

EJC Supplements, Vol 13, Iss 1, Pp 19-

2015 Volume 20

Abstract: The Epstein–Barr virus (EBV) represents an etiological agent for a number of human benign and malignant tumors. One of the EBV encoded proteins, the latent membrane protein 1 (LMP1), is involved in activation of many signaling pathways and transcription ... ...

Abstract	The Epstein–Barr virus (EBV) represents an etiological agent for a number of human benign and malignant tumors. One of the EBV encoded proteins, the latent membrane protein 1 (LMP1), is involved in activation of many signaling pathways and transcription factors leading EBV infected cells to immortalization and transformation. It’s well known that almost all worlds’ population is infected with EBV. As usually, infection occurs during early childhood without serious consequences for infected people. At the same time a secondary infection by additional EBV strain(s) occurs quite often. During the in vitro cultivation of peripheral blood lymphocyte from persons infected with multiple strains of the virus, only one of them having LMP1 oncogene with highest transforming potential becomes dominant while the others are eliminated. To figure out whether pattern of LMP1 expressions reflects the origin of EBV strains, six cell lines from patients with tumors, associated and not-associated with the virus and healthy individuals were established. The nucleotide and deductive amino acid (a.a.) sequences of LMP1 isolates tested were analyzed and compared with those of LMP1 isolates obtained from eight cell lines of African and Japanese EBV-associated Burkitt’s lymphomas (BL) origin. As the result, in four out of six cell lines of Russian origin (2 from patients with lymphoid pathology and 2 from PBLs of blood donors) the low divergent LMP1 B95.8/A variant characterized by a low transforming activity and a small number of a.a. substitutions was detected. For other two cell lines originated from EBV-associated patient with nasopharyngeal carcinoma and not virus-associated Hodgkin’s lymphoma patient the LMP1Med- and LMP1China1 variants, characterized by a larger set of mutations and high transforming potential, were found. Low divergent LMP1 variants (B95.8 or B95.8/A) were observed for 13 of 15 LMP1 samples from PBLs of Russian blood donors; in 2 donors highly divergent China1 and NC LMP1 variants were also detected. Among eight ...
Keywords	Medicine ; R ; Neoplasms. Tumors. Oncology. Including cancer and carcinogens ; RC254-282
Subject code	616
Language	English
Publishing date	2015-11-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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