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Article ; Online: Comparison of intracellular cytokine staining versus an ELISA-based assay to assess CMV-specific cell-mediated immunity in high-risk kidney transplant recipients.

Fernández-Ruiz, Mario / Redondo, Natalia / Parra, Patricia / Ruiz-Merlo, Tamara / Rodríguez-Goncer, Isabel / Polanco, Natalia / González, Esther / López-Medrano, Francisco / San Juan, Rafael / Navarro, David / Andrés, Amado / Aguado, José María

Journal of medical virology

2023 Volume 95, Issue 4, Page(s) e28733

Abstract: The best method for monitoring cytomegalovirus (CMV)-specific cell-mediated immunity (CMV-CMI) among high-risk kidney transplant (KT) recipients remains uncertain. We assessed CMV-CMI by intracellular cytokine staining (ICS) by flow cytometry and a ... ...

Abstract	The best method for monitoring cytomegalovirus (CMV)-specific cell-mediated immunity (CMV-CMI) among high-risk kidney transplant (KT) recipients remains uncertain. We assessed CMV-CMI by intracellular cytokine staining (ICS) by flow cytometry and a commercial interferon (IFN)-γ release assay (QuantiFERON®-CMV [QTF-CMV]) at posttransplant months 3, 4, and 5 in 53 CMV-seropositive KT recipients that had received induction therapy with antithymocyte globulin (ATG) and a 3-month course of valganciclovir prophylaxis. The discriminative capacity (areas under receiver operating characteristics curve [auROCs]) and diagnostic accuracy to predict immune protection against CMV infection from the discontinuation of prophylaxis to month 12 were compared between both methods. There was significant although moderate correlations between CMV-specific IFN-γ-producing CD8
MeSH term(s)	Humans ; Cytomegalovirus ; Kidney Transplantation/adverse effects ; Cytokines ; CD8-Positive T-Lymphocytes ; Cytomegalovirus Infections/diagnosis ; Cytomegalovirus Infections/prevention & control ; Immunity, Cellular ; Transplant Recipients ; Enzyme-Linked Immunosorbent Assay
Chemical Substances	Cytokines
Language	English
Publishing date	2023-04-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28733
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Article: Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA.

Tuells, José / Parra-Grande, Mónica / Santos-Calle, Francisco J / Montagud, Ana C / Egoavil, Cecilia M / García-Rivera, Celia / Caballero, Pablo / Gabaldón-Bravo, Eva M / Rodríguez-Diaz, Juan Carlos / Hurtado-Sánchez, José Antonio

Vaccines

2022 Volume 10, Issue 4

Abstract: ... the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral ...

Abstract	The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid diagnostic tests during population-based evaluations offers an opportunity to identify individuals with serologic evidence of natural infection or who have undergone vaccination. We carried out a cross-sectional study to assess the presence of neutralizing antibodies against SARS-CoV-2 among medical providers at an intensive care unit of a large referral hospital in Alicante, Spain. In addition, we tested for the presence of neutralizing antibodies compared to serum of uninfected individuals from a Biobank. We were also interested in evaluating the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral neutralization test (sVNT). This rapid test demonstrated a specificity of 1.000 95% CI (0.91-1.00) and the sensitivity of 0.987 95% CI (0.93-1.00). The negative predictive value was 95%. After six months, this rapid test demonstrated that those immunized with two doses of BioNTech/Pfizer vaccine, maintained optimal levels of neutralizing antibodies. We concluded that all Health Care Workers develop NAbs and the use of this rapid immunochromatographic test represents a potential tool to be used in population-based studies to detect serological antibody responses to vaccination. Vaccination policies could benefit from this tool to assess additional doses of vaccine or boosters among high-risk populations.
Language	English
Publishing date	2022-03-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10040510
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Article ; Online: Monitoring of CMV-specific cell-mediated immunity with a commercial ELISA-based interferon-γ release assay in kidney transplant recipients treated with antithymocyte globulin.

Fernández-Ruiz, Mario / Rodríguez-Goncer, Isabel / Parra, Patricia / Ruiz-Merlo, Tamara / Corbella, Laura / López-Medrano, Francisco / Polanco, Natalia / González, Esther / San Juan, Rafael / Folgueira, María Dolores / Andrés, Amado / Aguado, Jose María

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

2020 Volume 20, Issue 8, Page(s) 2070–2080

Abstract: ... ELISA-based interferon (IFN)-γ release assay (QTF-CMV) from posttransplant months 2-5 (362 points ...

Abstract	Monitoring for cytomegalovirus (CMV)-specific cell-mediated immunity (CMV-CMI) may be useful for individualizing valganciclovir (VGCV) prophylaxis after kidney transplantation (KT). We performed a commercial ELISA-based interferon (IFN)-γ release assay (QTF-CMV) from posttransplant months 2-5 (362 points) in 120 CMV-seropositive KT recipients that received antithymocyte globulin as induction therapy and VGCV prophylaxis (median of 92 days). Forty-seven patients (39.3%) had CMV infection after discontinuation of prophylaxis. The QTF-CMV assay was reactive, nonreactive, and indeterminate in 264 (72.9%), 90 (24.9%), and 8 points (2.2%). The QTF-CMV assay at prophylaxis discontinuation exhibited suboptimal accuracy for predicting protective CMV-CMI (sensitivity: 77.4%; specificity: 34.3%; positive predictive value [PPV]: 64.1%; negative predictive value [NPV]: 50.0%), with no differences in 1-year CMV infection rates between patients with negative (nonreactive or indeterminate) or reactive results (45.8% vs 36.1%; P = .244). Specificity and PPV to predict protective CMV-CMI improved by elevating the IFN-γ cutoff value to 1.13 IU/mL (65.7% and 71.4%) and 7.0 IU/mL (85.7% and 76.2%), although NPVs decreased. The QTF-CMV assay as per manufacturer's interpretative criteria performed poorly to predict protection from CMV infection following discontinuation of VGCV prophylaxis among ATG-treated CMV-seropositive KT recipients. This performance is slightly improved by modifying the IFN-γ positivity threshold.
MeSH term(s)	Antilymphocyte Serum/therapeutic use ; Antiviral Agents/therapeutic use ; Cytomegalovirus ; Cytomegalovirus Infections/diagnosis ; Cytomegalovirus Infections/drug therapy ; Cytomegalovirus Infections/prevention & control ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunity, Cellular ; Interferon-gamma Release Tests ; Kidney Transplantation/adverse effects ; Transplant Recipients
Chemical Substances	Antilymphocyte Serum ; Antiviral Agents
Language	English
Publishing date	2020-02-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2060594-8
ISSN	1600-6143 ; 1600-6135
ISSN (online)	1600-6143
ISSN	1600-6135
DOI	10.1111/ajt.15793
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Article ; Online: ELISA for detection of variant rabbit haemorrhagic disease virus RHDV2 antigen in liver extracts.

Dalton, K P / Podadera, A / Granda, V / Nicieza, I / Del Llano, D / González, R / de Los Toyos, J R / García Ocaña, M / Vázquez, F / Martín Alonso, J M / Prieto, J M / Parra, F / Casais, R

Journal of virological methods

2018 Volume 251, Page(s) 38–42

Abstract: ... a specific sandwich enzyme-linked immunosorbent assay (ELISA) for detection of RHDV2 antigens in rabbit liver ... an anti-RHDV2 goat polyclonal antibody (Pab), was developed. This ELISA was able to successfully detect ... samples analyzed. The ELISA afforded good repeatability and had high analytical sensitivity as it was able ...

Abstract	The emergence and rapid spread of variant of the rabbit hemorrhagic disease virus (RHDV2) require new diagnostic tools to ensure that efficient control measures are adopted. In the present study, a specific sandwich enzyme-linked immunosorbent assay (ELISA) for detection of RHDV2 antigens in rabbit liver homogenates, based on the use of an RHDV2-specific monoclonal antibody (Mab) 2D9 for antigen capture and an anti-RHDV2 goat polyclonal antibody (Pab), was developed. This ELISA was able to successfully detect RHDV2 and RHDV2 recombinant virions with high sensitivity (100%) and specificity (97.22%). No cross-reactions were detected with RHDV G1 viruses while low cross-reactivity was detected with one of the RHDVa samples analyzed. The ELISA afforded good repeatability and had high analytical sensitivity as it was able to detect a dilution 1:163,640 (6.10ng/mL) of purified RHDV-N11 VLPs, which contained approximately 3.4×10
Keywords	covid19
Language	English
Publishing date	2018-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2017.09.019
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Article ; Online: Detection of Neutralizing Antibodies against SARS-CoV-2 Post-Vaccination in Health Care Workers of a Large Tertiary Hospital in Spain by Using a Rapid Test LFIC and sVNT-ELISA

José Tuells / Mónica Parra-Grande / Francisco J. Santos-Calle / Ana C. Montagud / Cecilia M. Egoavil / Celia García-Rivera / Pablo Caballero / Eva M. Gabaldón-Bravo / Juan Carlos Rodríguez-Diaz / José Antonio Hurtado-Sánchez

Vaccines, Vol 10, Iss 510, p

2022 Volume 510

Abstract: ... the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral ...

Abstract	The presence of neutralizing antibodies (NAbs) against SARS-CoV-2 represent a surrogate marker of immunologic protection in populations at high risk of infection such as healthcare workers caring for hospitalized patients with COVID-19. As recommended by CDC and the European CDC, the use of rapid diagnostic tests during population-based evaluations offers an opportunity to identify individuals with serologic evidence of natural infection or who have undergone vaccination. We carried out a cross-sectional study to assess the presence of neutralizing antibodies against SARS-CoV-2 among medical providers at an intensive care unit of a large referral hospital in Alicante, Spain. In addition, we tested for the presence of neutralizing antibodies compared to serum of uninfected individuals from a Biobank. We were also interested in evaluating the use of a rapid lateral flow immunochromatography (LFIC) test against a surrogate ELISA viral neutralization test (sVNT). This rapid test demonstrated a specificity of 1.000 95% CI (0.91–1.00) and the sensitivity of 0.987 95% CI (0.93–1.00). The negative predictive value was 95%. After six months, this rapid test demonstrated that those immunized with two doses of BioNTech/Pfizer vaccine, maintained optimal levels of neutralizing antibodies. We concluded that all Health Care Workers develop NAbs and the use of this rapid immunochromatographic test represents a potential tool to be used in population-based studies to detect serological antibody responses to vaccination. Vaccination policies could benefit from this tool to assess additional doses of vaccine or boosters among high-risk populations.
Keywords	neutralizing antibodies ; COVID-19 ; SARS-CoV-2 ; serological test ; immunoassay ; lateral flow assay ; Medicine ; R
Subject code	360
Language	English
Publishing date	2022-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: EVALUACIÓN SEROLÓGICA Y CLÍNICO PATOLÓGICA A TRAVÉS DE LAS PRUEBAS DE IGDA y ELISA EN CARNEROS INOCULADOS EXPERIMENTALMENTE CON Brucella ovis.

Víctor Manuel Sánchez Parra / Edgardo Soriano Vargas / Claudia Giovanna Peñuelas Rivas / Ruy Ortiz Rodríguez / Fernando Alberto Paolicchi / Valente Velázquez Ordoñez / Martín Talavera Rojas / Jorge Acosta Dibarrat

Revista Científica, Vol 28, Iss

2019 Volume 3

Abstract: ... de Inmunodifusión en Gel de Agar (IGDA) y ELISA. El semen se procesó por la técnica de reacción en cadena ... de la polimerasa PCR. El análisis estadístico se realizó mediante modelos categóricos y mediciones repetidas. ELISA ...

Abstract	El objetivo de este estudio fue caracterizar los hallazgos patológicos y su relación con los resultados clínicos y serológicos en carneros infectados experimentalmente (IE) con Brucella ovis. Se utilizaron 18 carneros de 1 a 4 años y libres de B. ovis; que se distribuyeron en tres grupos: Grupo 1 (n= 6): inoculación en mucosas (B. ovis); Grupo 2 (n= 6), inoculación vía intravenosa y Grupo 3 (n= 6) Control. Se llevó a cabo el seguimiento de un carnero -1 grupo durante 189 días (d) post-IE. Se obtuvieron muestras de 2 mL de suero sanguíneo y semen entre los días 0 y 189 post-IE para realizar pruebas serológicas de Inmunodifusión en Gel de Agar (IGDA) y ELISA. El semen se procesó por la técnica de reacción en cadena de la polimerasa PCR. El análisis estadístico se realizó mediante modelos categóricos y mediciones repetidas. ELISA mostró mayor sensibilidad (P<0,05) para la detección de carneros seropositivos (CS) a partir del d 3 post-IE, a los 21 d post-IE, alcanzó su máxima detección de CS (100%) en ambos grupos IE (P<0,05). En el d 56 post-IE, la tasa de CS comenzó a descender (P<0,05). En 83% de los CS no -1 se logró el aislamiento de B. ovis por cultivo bacteriano ni PCR. Se presentaron lesiones induradas: 50% en testículo derecho y 33,3% en testículo izquierdo, en carneros con IE vía endovenosa. En este grupo, el 16,6% de CS presentaron adherencias y granulomas en cabeza, cuerpo y cola del epidídimo derecho. La utilización de la prueba de IGDA, la cual es la establecida por la Norma Oficial Mexicana (NOM-041-ZOO-1995) imposibilitaría la detección temprana de animales seropositivos lo que restaría eficacia a los programas de control de la enfermedad.
Keywords	Epididimitis ovina ; Brucella ovis ; IGDA ; ELISA ; Cattle ; SF191-275 ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2019-11-01T00:00:00Z
Publisher	Universidad del Zulia
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Feather corticosterone evaluated by ELISA in broilers: a potential tool to evaluate broiler welfare.

Carbajal, A / Tallo-Parra, O / Sabes-Alsina, M / Mular, I / Lopez-Bejar, M

Poultry science

2014 Volume 93, Issue 11, Page(s) 2884–2886

Abstract: ... CORT in broiler feathers by ELISA, which had never been done before, and an assay validation test was ... adrenal activity. Results indicate that ELISA is a valid tool to detect feather CORT levels in broilers. ...

Abstract	The measure of corticosterone (CORT) in feathers has been recently recognized as a valid and easily obtainable measure of chronic glucocorticoids secretion in avian species. This measure provides meaningful interpretations of how individuals respond to environmental perturbations. The growing interest of the public toward animal-food production welfare shows the need for improving and expanding objective tools to evaluate this issue. The present study evaluates whether it is possible to detect CORT in broiler feathers, and thus, assess if it would be a useful measure to study broiler welfare. Twenty-two broilers were randomly selected from an intensive farm. Four to 6 dorsal feathers were collected from each bird, and sex, weight, and morphological aspects of feather status were recorded. We tested the feasibility for detecting CORT in broiler feathers by ELISA, which had never been done before, and an assay validation test was performed. No significant relationships were found between feather CORT concentrations and physiological variables such as sex, weight, and fault bars in broilers. To our knowledge, this is the first study that uses broiler feathers as a matrix that provides a retrospective record of their hypothalamic-pituitary-adrenal activity. Results indicate that ELISA is a valid tool to detect feather CORT levels in broilers.
MeSH term(s)	Animal Welfare ; Animals ; Chickens/metabolism ; Corticosterone/blood ; Corticosterone/metabolism ; Enzyme-Linked Immunosorbent Assay/veterinary ; Feathers/chemistry ; Female ; Hypothalamo-Hypophyseal System/physiology ; Male ; Pituitary-Adrenal System/physiology ; Stress, Physiological
Chemical Substances	Corticosterone (W980KJ009P)
Language	English
Publishing date	2014-09-05
Publishing country	England
Document type	Journal Article ; Validation Study
ZDB-ID	242586-5
ISSN	1525-3171 ; 0032-5791
ISSN (online)	1525-3171
ISSN	0032-5791
DOI	10.3382/ps.2014-04092
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Article: Validation of a novel ELISA for measurement of electronegative low-density lipoprotein.

Santo Faulin, Tanize do Espírito / de Sena, Karine Cavalcanti Maurício / Rodrigues Telles, Andréia Elisa / de Mattos Grosso, Daniela / Bernardi Faulin, Edson José / Parra Abdalla, Dulcineia Saes

Clinical chemistry and laboratory medicine

2008 Volume 46, Issue 12, Page(s) 1769–1775

Abstract: ... Methods: We developed and validated a sandwich ELISA for LDL(-) in human plasma using two ... sample dilution. ELISA validation showed intra- and inter-assay precision and recovery ... This ELISA represents a very practical tool for measuring LDL(-) in human blood for widespread research and ...

Abstract	Background: Oxidative modification of low-density lipoprotein (LDL) plays a key role in the pathogenesis of atherosclerosis. LDL(-) is present in blood plasma of healthy subjects and at higher concentrations in diseases with high cardiovascular risk, such as familial hypercholesterolemia or diabetes. Methods: We developed and validated a sandwich ELISA for LDL(-) in human plasma using two monoclonal antibodies against LDL(-) that do not bind to native LDL, extensively copper-oxidized LDL or malondialdehyde-modified LDL. The characteristics of assay performance, such as limits of detection and quantification, accuracy, inter- and intra-assay precision were evaluated. The linearity, interferences and stability tests were also performed. Results: The calibration range of the assay is 0.625-20.0 mU/L at 1:2000 sample dilution. ELISA validation showed intra- and inter-assay precision and recovery within the required limits for immunoassays. The limits of detection and quantification were 0.423 mU/L and 0.517 mU/L LDL(-), respectively. The intra- and inter-assay coefficient of variation ranged from 9.5% to 11.5% and from 11.3% to 18.9%, respectively. Recovery of LDL(-) ranged from 92.8% to 105.1%. Conclusions: This ELISA represents a very practical tool for measuring LDL(-) in human blood for widespread research and clinical sample use.
MeSH term(s)	Chromatography, Ion Exchange ; Electrochemistry ; Enzyme-Linked Immunosorbent Assay/methods ; Enzyme-Linked Immunosorbent Assay/standards ; Humans ; Lipoproteins, LDL/blood ; Reproducibility of Results
Chemical Substances	Lipoproteins, LDL
Language	English
Publishing date	2008
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
ZDB-ID	1418007-8
ISSN	1437-4331 ; 1434-6621 ; 1437-8523
ISSN (online)	1437-4331
ISSN	1434-6621 ; 1437-8523
DOI	10.1515/CCLM.2008.333
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Article: ELISA for detection of variant rabbit haemorrhagic disease virus RHDV2 antigen in liver extracts

Dalton, K.P / A. Podadera / V. Granda / I. Nicieza / D. del Llano / R. GonzÃ¡lez / J.R. de los Toyos / M. GarcÃa OcaÃ±a / F. VÃ¡zquez / J.M. Martin Alonso / J.M. Prieto / F. Parra / R. Casais

Journal of virological methods. 2017,

2017

Abstract	The emergence and rapid spread of variant of the rabbit hemorrhagic disease virus (RHDV2) require new diagnostic tools to ensure that efficient control measures are adopted.In the present study, a specific sandwich enzyme-linked immunosorbent assay (ELISA) for detection of RHDV2 antigens in rabbit liver homogenates, based on the use of an RHDV2-specific monoclonal antibody (Mab) 2D9 for antigen capture and an anti-RHDV2 goat polyclonal antibody (Pab), was developed. This ELISA was able to successfully detect RHDV2 and RHDV2 recombinant virions with high sensitivity (100%) and specificity (97.22%). No cross-reactions were detected with RHDV G1 viruses while low cross-reactivity was detected with one of the RHDVa samples analyzed. The ELISA afforded good repeatability and had high analytical sensitivity as it was able to detect a dilution 1:163,640 (6.10ng/mL) of purified RHDV-N11 VLPs, which contained approximately 3.4Ã108molecules/mL particles.The reliable discrimination between closely related viruses is crucial to understand the epidemiology and the interaction of co-existing pathogens. In the work described here we design and validate an ELISA for laboratory based, specific, sensitive and reliable detection of RHDV2/RHDV2. This ELISA is a valuable, specific virological tool for monitoring virus circulation, which will permit a better control of this disease.
Keywords	Rabbit hemorrhagic disease virus ; antigens ; control methods ; cross reaction ; detection limit ; diagnostic techniques ; enzyme-linked immunosorbent assay ; epidemiology ; goats ; liver ; monitoring ; monoclonal antibodies ; pathogens ; polyclonal antibodies ; rabbits ; virion ; viruses ; covid19
Language	English
Size	p. .
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2017.09.019
Database	NAL-Catalogue (AGRICOLA)

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Article: Autoantibody profiles in canine ANA-positive sera investigated by immunoblot and ELISA.

Welin Henriksson, E / Hansson, H / Karlsson-Parra, A / Pettersson, I

Veterinary immunology and immunopathology

1998 Volume 61, Issue 2-4, Page(s) 157–170

Abstract: ... recombinant U1-70K ELISA. We compared these results with a previously shown concordance between ...

Abstract	Canine systemic lupus erythematosus (SLE) has a similar disease expression as human SLE, but the serological characterisation of the canine disease is as yet incomplete. In the present study, we examined the specificity of antinuclear antibodies (ANA) in indirect immunofluorescence (IIF) positive canine sera. Sixty-four canine IIF ANA positive sera were characterised using HeLa cell nuclear extract immunoblots and recombinant U1-70K ELISA. We compared these results with a previously shown concordance between indirect immunofluorescence and immunodiffusion in canine SLE serological diagnosis. One canine serum reacting with Sm proteins was observed, and five canine sera presented anti-RNP autoantibodies against the antigens 70K, A, C, and/or B/B'. The autoantigen most frequently recognised was a 43 kDa nuclear protein, previously described as hnRNP G. This prominent canine autoantigen was missing in the commercially available extract designed for immunodiffusion testing of human sera. Other prominent canine autoantigens were found not to be identical with the principal human ones, thus making present human test systems deficient for the use in canine systemic connective disease diagnosis. The development of antigenic extract designed for canine autoimmune autoantigens is necessary in order to make immunodiffusion a useful method in canine diagnosis. The anti-RNP positive canine sera were examined in more detail and we found that the human major antigenic region of the most prominent RNP antigen, the U1-70K protein, also is targeted by canine autoantibodies. Thus, the response against the RNP antigen seems to be conserved between man and dog.
MeSH term(s)	Animals ; Antibodies, Antinuclear/blood ; Autoantigens ; Dog Diseases/immunology ; Dogs ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique, Indirect ; Heterogeneous-Nuclear Ribonucleoproteins ; Humans ; Immunoblotting ; Immunodiffusion ; Lupus Erythematosus, Systemic/immunology ; Lupus Erythematosus, Systemic/veterinary ; Ribonucleoprotein, U1 Small Nuclear/immunology ; Ribonucleoproteins/immunology ; Ribonucleoproteins, Small Nuclear ; Species Specificity ; snRNP Core Proteins
Chemical Substances	Antibodies, Antinuclear ; Autoantigens ; Heterogeneous-Nuclear Ribonucleoproteins ; Ribonucleoprotein, U1 Small Nuclear ; Ribonucleoproteins ; Ribonucleoproteins, Small Nuclear ; SNRNP70 protein, human ; snRNP Core Proteins
Language	English
Publishing date	1998-06-03
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	754160-0
ISSN	1873-2534 ; 0165-2427
ISSN (online)	1873-2534
ISSN	0165-2427
DOI	10.1016/s0165-2427(97)00142-6
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