Article ; Online: Glutamate Spillover Dynamically Strengthens Gabaergic Synaptic Inhibition of the Hypothalamic Paraventricular Nucleus.
The Journal of neuroscience : the official journal of the Society for Neuroscience
2024 Volume 44, Issue 7
Abstract: The hypothalamic paraventricular nucleus (PVN) is strongly inhibited by γ-aminobutyric acid (GABA) from the surrounding peri-nuclear zone (PNZ). Because glutamate mediates fast excitatory transmission and is substrate for GABA synthesis, we tested its ... ...
Abstract | The hypothalamic paraventricular nucleus (PVN) is strongly inhibited by γ-aminobutyric acid (GABA) from the surrounding peri-nuclear zone (PNZ). Because glutamate mediates fast excitatory transmission and is substrate for GABA synthesis, we tested its capacity to dynamically strengthen GABA inhibition. In PVN slices from male mice, bath glutamate applied during ionotropic glutamate receptor blockade increased PNZ-evoked inhibitory postsynaptic currents (eIPSCs) without affecting GABA-A receptor agonist currents or single-channel conductance, implicating a presynaptic mechanism(s). Consistent with this interpretation, bath glutamate failed to strengthen IPSCs during pharmacological saturation of GABA-A receptors. Presynaptic analyses revealed that glutamate did not affect paired-pulse ratio, peak eIPSC variability, GABA vesicle recycling speed, or readily releasable pool (RRP) size. Notably, glutamate-GABA strengthening (GGS) was unaffected by metabotropic glutamate receptor blockade and graded external Ca |
---|---|
MeSH term(s) | Male ; Mice ; Animals ; Paraventricular Hypothalamic Nucleus/metabolism ; Glutamic Acid/metabolism ; Neurons/physiology ; gamma-Aminobutyric Acid/metabolism ; Neuroglia/metabolism ; Synaptic Transmission/physiology |
Chemical Substances | Glutamic Acid (3KX376GY7L) ; gamma-Aminobutyric Acid (56-12-2) |
Language | English |
Publishing date | 2024-02-14 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 604637-x |
ISSN | 1529-2401 ; 0270-6474 |
ISSN (online) | 1529-2401 |
ISSN | 0270-6474 |
DOI | 10.1523/JNEUROSCI.1851-22.2023 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.A 1668: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.