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Article ; Online: Gut bacteria promote proliferation in benign S/RG/C2 colorectal tumour cells, and promote proliferation, migration and invasion in malignant HCT116 cells.

Robson, J L / Thorn, R M S / Williams, A C / Collard, T J / Qualtrough, D

2023 Volume 13, Issue 1, Page(s) 17291

Abstract: Colorectal cancer (CRC) is a significant global health burden with a rising incidence worldwide. Distinct bacterial populations are associated with CRC development and progression, and it is thought that the relationship between CRC and associated gut ... ...

Abstract	Colorectal cancer (CRC) is a significant global health burden with a rising incidence worldwide. Distinct bacterial populations are associated with CRC development and progression, and it is thought that the relationship between CRC and associated gut bacteria changes during the progression from normal epithelium to benign adenoma and eventually malignant carcinoma and metastasis. This study compared the interaction of CRC-associated species Enterotoxigenic Bacteroides fragilis, Enterococcus faecalis and Fusobacterium nucleatum and one probiotic species, Escherichia coli Nissle 1917 with a colorectal adenoma (S/RG/C2) and a colorectal adenocarcinoma (HCT116) derived cell line. Gentamicin protection assays showed that all species displayed higher attachment to benign tumour monolayers when compared to malignant monolayers. However, invasion of 3/4 species was higher in the HCT116 cells than in the adenoma cells. All species were found to persist within tumour cell monolayers for a minimum of 48 h under standard aerobic cell culture conditions, with persistence significantly higher in HCT116 cells. Downstream assays were performed to analyse the behaviour of S/RG/C2 and HCT116 cells post-infection and revealed that all species increased the tumour cell yield of both cell lines. The migratory and invasive potential of HCT116 cells was increased after infection with F. nucleatum; however, no species significantly altered these characteristics in S/RG/C2 cells. These results add to the growing evidence for the involvement of microorganisms in CRC progression and suggest that these interactions may be dependent on tumour cell-specific characteristics.
MeSH term(s)	Humans ; HCT116 Cells ; Colorectal Neoplasms/pathology ; Bacteria ; Cell Proliferation ; Adenoma/pathology
Language	English
Publishing date	2023-10-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-44130-6
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Re Re : laparoscopic direct feeding jejunostomy.

Collard, M K / Genser, L / Vaillant, J C

Journal of visceral surgery

2020 Volume 157, Issue 2, Page(s) 167–168

MeSH term(s)	Enteral Nutrition ; Humans ; Jejunostomy ; Laparoscopy
Language	English
Publishing date	2020-01-17
Publishing country	France
Document type	Letter ; Comment
ISSN	1878-7886
ISSN (online)	1878-7886
DOI	10.1016/j.jviscsurg.2020.01.004
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Article ; Online: Percutaneous-Endoscopic Rendezvous Procedure in a Pediatric Patient With Biliary Obstruction and Altered Anatomy.

Mauer, Tian L / Trocchia, Carolena / Collard, Michael / Chandler, Nicole M / Poulos, Nicholas / Wilsey, Michael

Journal of pediatric gastroenterology and nutrition

2023 Volume 77, Issue 4, Page(s) e67

MeSH term(s)	Humans ; Child ; Cholestasis/etiology ; Cholestasis/surgery ; Cholangiopancreatography, Endoscopic Retrograde/methods ; Drainage/methods
Language	English
Publishing date	2023-05-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	603201-1
ISSN	1536-4801 ; 0277-2116
ISSN (online)	1536-4801
ISSN	0277-2116
DOI	10.1097/MPG.0000000000003842
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Zs.A 1728: Show issues

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Article ; Online: Direct laparoscopic feeding jejunostomy.

Collard, M K / Genser, L / Vaillant, J C

Journal of visceral surgery

2019 Volume 156, Issue 5, Page(s) 433–439

MeSH term(s)	Enteral Nutrition/methods ; Humans ; Jejunostomy/methods ; Laparoscopy/methods
Language	English
Publishing date	2019-10-04
Publishing country	France
Document type	Journal Article
ISSN	1878-7886
ISSN (online)	1878-7886
DOI	10.1016/j.jviscsurg.2019.07.005
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Article: Syndrome néphrotique congénital sévère avec mutation NPH S1.

Quiros, A / Lefèbvre, C / Collard, L / Rigo, V / Lombet, J

Revue medicale de Liege

2020 Volume 75, Issue 7-8, Page(s) 544–547

Abstract: The congenital nephrotic syndrome is a rare and severe pathology, and its management represents a real challenge for pediatric nephrologists. We report the case of a congenital nephrotic syndrome secondary to a homozygous mutation of the NPHS1. The young ...

Title translation	A case of severe congenital nephrotic syndrome secondary to NPHS1 mutation.
Abstract	The congenital nephrotic syndrome is a rare and severe pathology, and its management represents a real challenge for pediatric nephrologists. We report the case of a congenital nephrotic syndrome secondary to a homozygous mutation of the NPHS1. The young patient has a severe clinical course, and benefits of a management by anti-proteinuric treatment and a unilateral nephrectomy. This clinical case illustrates the difficulties of the management of a severe congenital nephrotic syndrome. To date, it is difficult to identify these patients beforehand because there is a poor correlation between the genotype and the phenotype of the NPHS1 mutation. There are two managements described in the literature: an early bilateral nephrectomy at 7 kg of weight with a renal transplant around 10 kg, versus a conservative management via an anti-proteinuric treatment and/or an unilateral nephrectomy. Current evidence is based on retrospective studies and the choice of a conservative approach versus early bilateral nephrectomy should take into account the severity of protein loss and its complications.
MeSH term(s)	Child ; Humans ; Infant ; Membrane Proteins/genetics ; Mutation ; Nephrotic Syndrome ; Phenotype ; Retrospective Studies
Chemical Substances	Membrane Proteins
Language	French
Publishing date	2020-08-11
Publishing country	Belgium
Document type	Case Reports
ZDB-ID	414001-1
ISSN	0370-629X ; 0035-3663
ISSN	0370-629X ; 0035-3663
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Zs.A 597: Show issues

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Article ; Online: Patient experience and physiological response to two commercially available daily disposable myopia control contact lenses.

Ghorbani-Mojarrad, Neema / Cargill, Catherine / Collard, Sophie / Terry, Louise

Contact lens & anterior eye : the journal of the British Contact Lens Association

2021 Volume 45, Issue 2, Page(s) 101426

Abstract: Background: A range of myopia management (MM) contact lenses are becoming available to practitioners. These lenses are designed to slow myopia progression and axial elongation. This study explored the initial experience of participants wearing daily ... ...

Abstract	Background: A range of myopia management (MM) contact lenses are becoming available to practitioners. These lenses are designed to slow myopia progression and axial elongation. This study explored the initial experience of participants wearing daily disposable MM contact lenses to investigate established factors previously associated with successful lens wear. Methods: This was a prospective, double-masked, crossover study. Twenty participants aged 18-30 years old were assigned to wear two daily disposable MM lenses in a randomised order. Visual acuity, contrast sensitivity, and amplitude/lag of accommodation were assessed at baseline, post-insertion, and after 2 and 6 h of lens wear. Self-reported lens comfort and vision quality were recorded at the same timepoints, and at 10 h post-insertion. Pairwise comparisons were performed between the two lenses at each timepoint, as well as assessing changes throughout wear. The relationship of the measured parameters to overall lens satisfaction was also assessed. Results: There were no significant differences between the two MM lenses at any timepoint for any of the participant-reported parameters, including overall satisfaction. A small difference in visual acuity was noted at 6 h post-insertion, although this is unlikely to be clinically significant. Comfort decreased throughout the day, most notably at 10 h post-insertion. A moderate positive correlation was observed between participant-reported visual quality and overall satisfaction. A similar pattern was seen for comfort and overall satisfaction. Self-reported vision quality and measured visual acuity were poorly correlated, highlighting the benefit of subjectively assessing the quality of vision with these lenses. Conclusions: The participants demonstrated comparable measures across a range of measures between the two MM lenses. Notably, half of the participants demonstrated a clear lens preference, although the preferred lens varied between individuals. Candidates for MM may benefit from trialling more than one MM lens design, to maximise initial wearing satisfaction.
MeSH term(s)	Adolescent ; Adult ; Contact Lenses, Hydrophilic ; Cross-Over Studies ; Disposable Equipment ; Humans ; Myopia/diagnosis ; Myopia/therapy ; Patient Outcome Assessment ; Patient Satisfaction ; Prospective Studies ; Young Adult
Language	English
Publishing date	2021-02-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2004847-6
ISSN	1476-5411 ; 1367-0484
ISSN (online)	1476-5411
ISSN	1367-0484
DOI	10.1016/j.clae.2021.02.012
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Article ; Online: Autonomic regulation in different hypertensive phenotypes - the HELIUS study.

Collard, D / Vriend, E M C / Galenkamp, H / Moll van Charante, E P / Vogt, L / Westerhof, B E / van den Born, B J H

Blood pressure

2023 Volume 32, Issue 1, Page(s) 2270070

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Male ; Humans ; Female ; Aged ; Adolescent ; Young Adult ; Adult ; Middle Aged ; Hypertension ; Blood Pressure/physiology ; Heart
Language	English
Publishing date	2023-10-20
Publishing country	England
Document type	Journal Article
ZDB-ID	1170048-8
ISSN	1651-1999 ; 1651-2480 ; 0803-7051 ; 0803-8023
ISSN (online)	1651-1999 ; 1651-2480
ISSN	0803-7051 ; 0803-8023
DOI	10.1080/08037051.2023.2270070
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Zs.A 3843: Show issues

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Article ; Online: Extracting patient-level data from the electronic health record: Expanding opportunities for health system research.

Farrand, Erica / Collard, Harold R / Guarnieri, Michael / Minowada, George / Block, Lawrence / Lee, Mei / Iribarren, Carlos

PloS one

2023 Volume 18, Issue 3, Page(s) e0280342

Abstract: Background: Epidemiological studies of interstitial lung disease (ILD) are limited by small numbers and tertiary care bias. Investigators have leveraged the widespread use of electronic health records (EHRs) to overcome these limitations, but struggle ... ...

Abstract	Background: Epidemiological studies of interstitial lung disease (ILD) are limited by small numbers and tertiary care bias. Investigators have leveraged the widespread use of electronic health records (EHRs) to overcome these limitations, but struggle to extract patient-level, longitudinal clinical data needed to address many important research questions. We hypothesized that we could automate longitudinal ILD cohort development using the EHR of a large, community-based healthcare system. Study design and methods: We applied a previously validated algorithm to the EHR of a community-based healthcare system to identify ILD cases between 2012-2020. We then extracted disease-specific characteristics and outcomes using fully automated data-extraction algorithms and natural language processing of selected free-text. Results: We identified a community cohort of 5,399 ILD patients (prevalence = 118 per 100,000). Pulmonary function tests (71%) and serologies (54%) were commonly used in the diagnostic evaluation, whereas lung biopsy was rare (5%). IPF was the most common ILD diagnosis (n = 972, 18%). Prednisone was the most commonly prescribed medication (911, 17%). Nintedanib and pirfenidone were rarely prescribed (n = 305, 5%). ILD patients were high-utilizers of inpatient (40%/year hospitalized) and outpatient care (80%/year with pulmonary visit), with sustained utilization throughout the post-diagnosis study period. Discussion: We demonstrated the feasibility of robustly characterizing a variety of patient-level utilization and health services outcomes in a community-based EHR cohort. This represents a substantial methodological improvement by alleviating traditional constraints on the accuracy and clinical resolution of such ILD cohorts; we believe this approach will make community-based ILD research more efficient, effective, and scalable.
MeSH term(s)	Humans ; Electronic Health Records ; Lung Diseases, Interstitial/diagnosis ; Lung ; Algorithms
Language	English
Publishing date	2023-03-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0280342
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Article ; Online: Rectovaginal Fistula: What is the Role of Martius Flap and Gracilis Muscle Interposition in the Therapeutic Strategy?

Pastier, Clément / Loriau, Jérôme / Denost, Quentin / O'Connell, Lauren / Challine, Alexandre / Collard, Maxime / Debove, Clotilde / Chafai, Najim / Parc, Yann / Lefevre, Jérémie H

Diseases of the colon and rectum

2024

Abstract: Background: While numerous treatments exist for management of rectovaginal fistula, none has demonstrated its superiority. The role of diverting stoma remains controversial. Few series include Martius flap in the armamentarium.: Objective: Determine ... ...

Abstract	Background: While numerous treatments exist for management of rectovaginal fistula, none has demonstrated its superiority. The role of diverting stoma remains controversial. Few series include Martius flap in the armamentarium. Objective: Determine the role of gracilis muscle interposition and Martius flap in the surgical management of rectovaginal fistula. Design: Retrospective cohort study of a pooled prospectively maintained database from 3 centers. Settings/patients: All consecutive eligible patients with rectovaginal fistula undergoing Martius flap and gracilis muscle interposition were included from 2001 to 2022. Main outcomes: Success was defined by absence of stoma and rectovaginal fistula. Results: Sixty-two patients were included with 55 Martius flap and 24 gracilis muscle interposition performed after failures of 164 initial procedures. Total length of stay was longer for gracilis muscle interposition by 2 days (p = 0.01) without a significant difference in severe morbidity (20% vs. 12%, p = 0.53). 27% of Martius flap were performed without stoma, without impact on overall morbidity (p = 0.763). Per-patient immediate success rates were not significantly different between groups (35% vs. 31%, p = 1.0). Success of gracilis muscle interposition after failure of Martius flap was not significantly different from an initial gracilis muscle interposition (p = 1.0). The immediate success rate rose to 49.4% (49% vs. 50%, p = 1.0) after simple perineal procedures. After a median follow-up of 23 months, there was no significant difference detected in success rate between the two procedures (69% vs. 69%, p = 1.0). Smoking was the only negative predictive factor (p = 0.02). Limitations: By its retrospective nature, this study is limited in its comparison. Conclusion: This novel comparison between Martius flap and gracilis muscle interposition suggests that Martius flap presents several advantages, including shorter length of stay, similar morbidity, and success. Proximal diversion via a stoma for Martius flap does not appear mandatory. Gracilis muscle interposition could be reserved as a salvage procedure after Martius flap failure. See Video Abstract.
Language	English
Publishing date	2024-04-23
Publishing country	United States
Document type	Journal Article
ZDB-ID	212581-x
ISSN	1530-0358 ; 0012-3706
ISSN (online)	1530-0358
ISSN	0012-3706
DOI	10.1097/DCR.0000000000003148
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Uk I Zs.237: Show issues

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Article ; Online: Use of a large-bore 088 intracranial access support catheter for delivery of large intracranial devices: case series with the TracStar LDP in 125 cases.

Campos, Jessica K / Zarrin, David A / Meyer, Benjamen M / Khan, Muhammad Waqas / Laghari, Fahad J / Collard de Beaufort, Jonathan C / Amin, Gizal / Beaty, Narlin B / Bender, Matthew T / Suzuki, Shuichi / Colby, Geoffrey P / Lin, Li-Mei / Coon, Alexander L

Journal of neurointerventional surgery

2024

Abstract: Background: The delivery of neuroendovascular devices requires a robust proximal access platform. This demand has previously been met with a 6Fr long sheath (8Fr guide) that is placed in the proximal internal carotid artery (ICA) or vertebral artery ... ...

Abstract	Background: The delivery of neuroendovascular devices requires a robust proximal access platform. This demand has previously been met with a 6Fr long sheath (8Fr guide) that is placed in the proximal internal carotid artery (ICA) or vertebral artery segments. We share our experience with the first 0.088 inch 8Fr guide catheter designed for direct intracranial access. Methods: We retrospectively reviewed a prospectively maintained IRB-approved institutional database of the senior authors to identify all cases where the TracStar Large Distal Platform (LDP) was positioned within the intracranial vasculature, defined as within or distal to the petrous ICA, vertebral artery (V3) segments, or transverse sinus. Technical success was defined as safe placement of the TracStar LDP within or distal to the described distal vessel segments with subsequent complication-free device implantation. Results: Over the 41-month study period from January 2020 to June 2023, 125 consecutive cases were identified in whom the TracStar LDP was navigated into the intracranial vasculature for triaxial delivery of large devices, 0.027 inch microcatheter and greater, for aneurysm treatment (n=108, 86%), intracranial angioplasty/stenting (n=15, 12%), and venous sinus stenting (n=2, 1.6%). All cases used a direct select catheter technique for initial guide placement (no exchange). Posterior circulation treatments occurred in 14.4% (n=18) of cases. Technical success was achieved in 100% of cases. No vessel dissections occurred in any cases. Conclusion: The TracStar LDP is an 0.088 inch 8Fr guide catheter that can establish direct intracranial access with an acceptable safety profile. This can be achieved in a wide range of neurointerventional cases with a high rate of technical success.
Language	English
Publishing date	2024-02-28
Publishing country	England
Document type	Journal Article
ZDB-ID	2514982-9
ISSN	1759-8486 ; 1759-8478
ISSN (online)	1759-8486
ISSN	1759-8478
DOI	10.1136/jnis-2023-021054
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