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  1. Article ; Online: Intestinal cell diversity and treatment responses in a parasitic nematode at single cell resolution.

    Tyagi, Rahul / Rosa, Bruce A / Swain, Amanda / Artyomov, Maxim N / Jasmer, Douglas P / Mitreva, Makedonka

    BMC genomics

    2024  Volume 25, Issue 1, Page(s) 341

    Abstract: Background: Parasitic nematodes, significant pathogens for humans, animals, and plants, depend on diverse organ systems for intra-host survival. Understanding the cellular diversity and molecular variations underlying these functions holds promise for ... ...

    Abstract Background: Parasitic nematodes, significant pathogens for humans, animals, and plants, depend on diverse organ systems for intra-host survival. Understanding the cellular diversity and molecular variations underlying these functions holds promise for developing novel therapeutics, with specific emphasis on the neuromuscular system's functional diversity. The nematode intestine, crucial for anthelmintic therapies, exhibits diverse cellular phenotypes, and unraveling this diversity at the single-cell level is essential for advancing knowledge in anthelmintic research across various organ systems.
    Results: Here, using novel single-cell transcriptomics datasets, we delineate cellular diversity within the intestine of adult female Ascaris suum, a parasitic nematode species that infects animals and people. Gene transcripts expressed in individual nuclei of untreated intestinal cells resolved three phenotypic clusters, while lower stringency resolved additional subclusters and more potential diversity. Clusters 1 and 3 phenotypes displayed variable congruence with scRNA phenotypes of C. elegans intestinal cells, whereas the A. suum cluster 2 phenotype was markedly unique. Distinct functional pathway enrichment characterized each A. suum intestinal cell cluster. Cluster 2 was distinctly enriched for Clade III-associated genes, suggesting it evolved within clade III nematodes. Clusters also demonstrated differential transcriptional responsiveness to nematode intestinal toxic treatments, with Cluster 2 displaying the least responses to short-term intra-pseudocoelomic nematode intestinal toxin treatments.
    Conclusions: This investigation presents advances in knowledge related to biological differences among major cell populations of adult A. suum intestinal cells. For the first time, diverse nematode intestinal cell populations were characterized, and associated biological markers of these cells were identified to support tracking of constituent cells under experimental conditions. These advances will promote better understanding of this and other parasitic nematodes of global importance, and will help to guide future anthelmintic treatments.
    MeSH term(s) Humans ; Animals ; Caenorhabditis elegans ; Intestines ; Nematoda/genetics ; Gene Expression Profiling ; Anthelmintics/pharmacology ; Anthelmintics/therapeutic use
    Chemical Substances Anthelmintics
    Language English
    Publishing date 2024-04-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2041499-7
    ISSN 1471-2164 ; 1471-2164
    ISSN (online) 1471-2164
    ISSN 1471-2164
    DOI 10.1186/s12864-024-10203-7
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  2. Article: Cell Death and Transcriptional Responses Induced in Larvae of the Nematode

    Jasmer, Douglas P / Rosa, Bruce A / Mitreva, Makedonka

    Pharmaceuticals (Basel, Switzerland)

    2021  Volume 14, Issue 7

    Abstract: Establishing methods to investigate treatments that induce cell death in parasitic nematodes will promote experimental approaches to elucidate mechanisms and to identify prospective anthelmintics capable of inducing this outcome. Here, we extended recent ...

    Abstract Establishing methods to investigate treatments that induce cell death in parasitic nematodes will promote experimental approaches to elucidate mechanisms and to identify prospective anthelmintics capable of inducing this outcome. Here, we extended recent progress on a method to monitor cell death and to identify small molecule inhibitors in
    Language English
    Publishing date 2021-06-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2193542-7
    ISSN 1424-8247
    ISSN 1424-8247
    DOI 10.3390/ph14070598
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  3. Article: Zucchini Plants Alter Gene Expression and Emission of (

    Vitiello, Alessia / Molisso, Donata / Digilio, Maria Cristina / Giorgini, Massimo / Corrado, Giandomenico / Bruce, Toby J A / D'Agostino, Nunzio / Rao, Rosa

    Frontiers in plant science

    2021  Volume 11, Page(s) 592603

    Abstract: Zucchini ( ...

    Abstract Zucchini (
    Language English
    Publishing date 2021-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2020.592603
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  4. Article ; Online: Cell Death and Transcriptional Responses Induced in Larvae of the Nematode Haemonchus contortus by Toxins/Toxicants with Broad Phylogenetic Efficacy

    Douglas P. Jasmer / Bruce A. Rosa / Makedonka Mitreva

    Pharmaceuticals, Vol 14, Iss 598, p

    2021  Volume 598

    Abstract: Establishing methods to investigate treatments that induce cell death in parasitic nematodes will promote experimental approaches to elucidate mechanisms and to identify prospective anthelmintics capable of inducing this outcome. Here, we extended recent ...

    Abstract Establishing methods to investigate treatments that induce cell death in parasitic nematodes will promote experimental approaches to elucidate mechanisms and to identify prospective anthelmintics capable of inducing this outcome. Here, we extended recent progress on a method to monitor cell death and to identify small molecule inhibitors in Ascaris suum to Haemonchus contortus , a phylogenetically distant parasitic nematode of significance for both human and agricultural animal health. We utilized a diverse group of small molecule inhibitors referred to as nematode intestinal toxins/toxicants (NITs) coupled with motility, cytological and cell death assays to resolve gross effects on motility and individual cells and organ systems of two H. contortus larval stages in culture. Early transcriptional response evaluation identified NIT-responsive genes and pathways. The scope of death among cells in larvae varied among NITs but shared patterns with A. suum , despite the approach having some limitations due to characteristics of H. contortus larvae. Gene response patterns varied among NITs tested and provided information on the cell targets and pathways affected. Experimental NIT assays provide tools capable of inducing cell death in larval stages of parasitic nematodes, and can resolve many individual cells and organ systems in which cell death can be induced.
    Keywords nematode ; intestine ; anthelmintics ; microscopy ; pathology ; RNA-seq ; Medicine ; R ; Pharmacy and materia medica ; RS1-441
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Parent Perception of School Meals in the San Joaquin Valley during COVID-19: A Photovoice Project.

    Sohlberg, Tatum M / Higuchi, Emma C / Ordonez, Valeria M / Escobar, Gabriela V / De La Rosa, Ashley / Islas, Genoveva / Castro, Cecilia / Hecht, Kenneth / Hecht, Christina E / Bruce, Janine S / Patel, Anisha I

    Nutrients

    2023  Volume 15, Issue 5

    Abstract: School-based nutrition programs are crucial to reducing food insecurity. The COVID-19 pandemic adversely impacted students' school meal participation. This study seeks to understand parent views of school meals during COVID-19 to inform efforts to ... ...

    Abstract School-based nutrition programs are crucial to reducing food insecurity. The COVID-19 pandemic adversely impacted students' school meal participation. This study seeks to understand parent views of school meals during COVID-19 to inform efforts to improve participation in school meal programs. Photovoice methodology was used to explore parental perception of school meals in San Joaquin Valley, California, a region of predominately Latino farmworker communities. Parents in seven school districts photographed school meals for a one-week period during the pandemic and then participated in focus group discussions and small group interviews. Focus group discussions and small group interviews were transcribed, and data were analyzed using a team-based, theme-analysis approach. Three primary domains emerged: benefits of school meal distribution, meal quality and appeal, and perceived healthfulness. Parents perceived school meals as beneficial to addressing food insecurity. However, they noted that meals were unappealing, high in added sugar, and unhealthy, which led to discarded meals and decreased participation in the school meal program. The transition to grab-and-go style meals was an effective strategy for providing food to families during pandemic school closures, and school meals remain an important resource for families experiencing food insecurity. However, negative parental perceptions of the appeal and nutritional content of school meals may have decreased school meal participation and increased food waste that could persist beyond the pandemic.
    MeSH term(s) Humans ; Pandemics ; COVID-19 ; Refuse Disposal ; Meals ; Parents ; Food Services ; Perception
    Language English
    Publishing date 2023-02-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu15051087
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  6. Article ; Online: Horizontal gene transfer provides insights into the deep evolutionary history and biology of

    Zarlenga, Dante / Thompson, Peter / Mitreva, Makedonka / Rosa, Bruce A / Hoberg, Eric

    Food and waterborne parasitology

    2022  Volume 27, Page(s) e00155

    Abstract: Evolution involves temporal changes in the characteristics of a species that are subsequently propagated or rejected through natural selection. In the case of parasites, host switching also plays a prominent role in the evolutionary process. These ... ...

    Abstract Evolution involves temporal changes in the characteristics of a species that are subsequently propagated or rejected through natural selection. In the case of parasites, host switching also plays a prominent role in the evolutionary process. These changes are rooted in genetic variation and gene flow where genes may be deleted, mutated (sequence), duplicated, rearranged and/or translocated and then transmitted through vertical gene transfer. However, the introduction of new genes is not driven only by Mendelian inheritance and mutation but also by the introduction of DNA from outside a lineage in the form of horizontal gene transfer between donor and recipient organisms. Once introduced and integrated into the biology of the recipient, vertical inheritance then perpetuates the newly acquired genetic factor, where further functionality may involve co-option of what has become a pre-existing physiological capacity. Upon sequencing the
    Language English
    Publishing date 2022-04-18
    Publishing country Netherlands
    Document type Journal Article
    ISSN 2405-6766
    ISSN (online) 2405-6766
    DOI 10.1016/j.fawpar.2022.e00155
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  7. Article ; Online: NF-κB signaling deficiency in CD11c-expressing phagocytes mediates early inflammatory responses and enhances Mycobacterium tuberculosis control.

    Chauhan, Kuldeep S / Dunlap, Micah D / Akter, Sadia / Gupta, Ananya / Ahmed, Mushtaq / Rosa, Bruce A / Dela Peña, Noreen B / Mitreva, Makedonka / Khader, Shabaana A

    The Journal of infectious diseases

    2024  

    Abstract: Early innate immune responses play an important role in determining the protective outcome of Mycobacterium tuberculosis (Mtb) infection. Nuclear factor kappa B (NF-κB) signaling in immune cells regulates the expression of key downstream effector ... ...

    Abstract Early innate immune responses play an important role in determining the protective outcome of Mycobacterium tuberculosis (Mtb) infection. Nuclear factor kappa B (NF-κB) signaling in immune cells regulates the expression of key downstream effector molecules that mount early anti-mycobacterial responses. Using conditional knockout mice, we studied the effect of abrogation of NF-κB signaling in different myeloid cell types and its impact on Mtb infection. Our results show that absence of IKK2-mediated signaling in all myeloid cells resulted in increased susceptibility to Mtb infection. In contrast, absence of IKK2-mediated signaling specifically in CD11c+ myeloid cells induced early pro-inflammatory cytokine responses, enhanced the recruitment of myeloid cells and mediated early resistance to Mtb. Abrogation of IKK2 in MRP8-expressing neutrophils did not impact either disease pathology or Mtb control. Thus, we describe an early immunoregulatory role for NF-κB signaling in CD11c-expressing phagocytes, and a later protective role for NF-κB in LysM-expressing cells during Mtb infection.
    Language English
    Publishing date 2024-02-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiae060
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    In stock of ZB MED Cologne/Königswinter

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  8. Article ; Online: Carboxylates and alcohols production in an autotrophic hydrogen-based membrane biofilm reactor.

    Calvo, Diana C / Ontiveros-Valencia, Aura / Krajmalnik-Brown, Rosa / Torres, Cesar I / Rittmann, Bruce E

    Biotechnology and bioengineering

    2021  Volume 118, Issue 6, Page(s) 2338–2347

    Abstract: Microbiological conversion of ... ...

    Abstract Microbiological conversion of CO
    MeSH term(s) Alcohols/metabolism ; Autotrophic Processes ; Bacteria/metabolism ; Bacteria, Anaerobic/metabolism ; Biofilms ; Bioreactors ; Carbon/metabolism ; Carboxylic Acids/metabolism ; Hydrogen ; Industrial Microbiology ; Membranes ; Microbiota
    Chemical Substances Alcohols ; Carboxylic Acids ; Carbon (7440-44-0) ; Hydrogen (7YNJ3PO35Z)
    Language English
    Publishing date 2021-03-30
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.27745
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    In stock of ZB MED Cologne/Königswinter

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  9. Article ; Online: Gavage approach to oxygen supplementation with oxygen therapeutic Ox66™ in a hypoventilation rodent model of respiratory distress.

    Nugent, William H / Carr, Danuel A / MacBryde, Rosa / Bruce, Erica D / Song, Bjorn K

    Artificial cells, nanomedicine, and biotechnology

    2021  Volume 49, Issue 1, Page(s) 709–716

    Abstract: Acute respiratory distress syndrome (ARDS) features pulmonary dysfunction capable of causing life-threatening hypoxaemia. Ventilation and hyperoxic therapies force oxygen through dysfunctional alveoli but risk exacerbating damage. Ox66™ is an ingestible, ...

    Abstract Acute respiratory distress syndrome (ARDS) features pulmonary dysfunction capable of causing life-threatening hypoxaemia. Ventilation and hyperoxic therapies force oxygen through dysfunctional alveoli but risk exacerbating damage. Ox66™ is an ingestible, solid-state oxygen product designed for oxygen supplementation. Eighteen anaesthetized, ventilated rats were subjected to a 40% reduction in tidal volume to produce a hypoventilatory simulation of the hypoxia in ARDS (HV-ARDS). After 60 min, animals were randomized to receive either normal saline (Saline; volume control) or Ox66
    MeSH term(s) Animals ; Disease Models, Animal ; Humans ; Hypoventilation/metabolism ; Hypoventilation/physiopathology ; Hypoventilation/therapy ; Lung/metabolism ; Lung/physiopathology ; Male ; Oxygen/pharmacology ; Oxygen Inhalation Therapy ; Rats ; Rats, Sprague-Dawley ; Respiratory Distress Syndrome/metabolism ; Respiratory Distress Syndrome/physiopathology ; Respiratory Distress Syndrome/therapy
    Chemical Substances Oxygen (S88TT14065)
    Language English
    Publishing date 2021-12-10
    Publishing country England
    Document type Journal Article ; Video-Audio Media
    ZDB-ID 2723095-8
    ISSN 2169-141X ; 2169-1401
    ISSN (online) 2169-141X
    ISSN 2169-1401
    DOI 10.1080/21691401.2021.2013251
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    In stock of ZB MED Cologne/Königswinter

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  10. Article ; Online: Insulin protects acinar cells during pancreatitis by preserving glycolytic ATP supply to calcium pumps.

    Bruce, Jason I E / Sánchez-Alvarez, Rosa / Sans, Maria Dolors / Sugden, Sarah A / Qi, Nathan / James, Andrew D / Williams, John A

    Nature communications

    2021  Volume 12, Issue 1, Page(s) 4386

    Abstract: Acute pancreatitis (AP) is serious inflammatory disease of the pancreas. Accumulating evidence links diabetes with severity of AP, suggesting that endogenous insulin may be protective. We investigated this putative protective effect of insulin during ... ...

    Abstract Acute pancreatitis (AP) is serious inflammatory disease of the pancreas. Accumulating evidence links diabetes with severity of AP, suggesting that endogenous insulin may be protective. We investigated this putative protective effect of insulin during cellular and in vivo models of AP in diabetic mice (Ins2
    MeSH term(s) Acinar Cells/drug effects ; Acinar Cells/metabolism ; Acute Disease ; Adenosine Triphosphate/metabolism ; Animals ; Calcium/metabolism ; Calcium-Transporting ATPases/metabolism ; Ceruletide ; Diabetes Mellitus, Experimental/metabolism ; Fatty Acids, Monounsaturated ; Glycolysis/drug effects ; Insulin/metabolism ; Insulin/pharmacology ; Male ; Mice ; Mice, Knockout ; Pancreas/metabolism ; Pancreatitis/drug therapy ; Pancreatitis/metabolism ; Pancreatitis/pathology ; Protective Agents/pharmacology
    Chemical Substances Fatty Acids, Monounsaturated ; Ins2 protein, mouse ; Insulin ; Protective Agents ; palmitoleic acid (209B6YPZ4I) ; Ceruletide (888Y08971B) ; Adenosine Triphosphate (8L70Q75FXE) ; Calcium-Transporting ATPases (EC 7.2.2.10) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-07-19
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-021-24506-w
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