Article ; Online: TRPV1 enhances cholecystokinin signaling in primary vagal afferent neurons and mediates the central effects on spontaneous glutamate release in the NTS.
American journal of physiology. Cell physiology
2023 Volume 326, Issue 1, Page(s) C112–C124
Abstract: The gut peptide cholecystokinin (CCK) is released during feeding and promotes satiation by increasing excitation of vagal afferent neurons that innervate the upper gastrointestinal tract. Vagal afferent neurons express CCK1 receptors (CCK1Rs) in the ... ...
| Abstract | The gut peptide cholecystokinin (CCK) is released during feeding and promotes satiation by increasing excitation of vagal afferent neurons that innervate the upper gastrointestinal tract. Vagal afferent neurons express CCK1 receptors (CCK1Rs) in the periphery and at central terminals in the nucleus of the solitary tract (NTS). While the effects of CCK have been studied for decades, CCK receptor signaling and coupling to membrane ion channels are not entirely understood. Previous findings have implicated L-type voltage-gated calcium channels as well as transient receptor potential (TRP) channels in mediating the effects of CCK, but the lack of selective pharmacology has made determining the contributions of these putative mediators difficult. The nonselective ion channel transient receptor potential vanilloid subtype 1 (TRPV1) is expressed throughout vagal afferent neurons and controls many forms of signaling, including spontaneous glutamate release onto NTS neurons. Here we tested the hypothesis that CCK1Rs couple directly to TRPV1 to mediate vagal signaling using fluorescent calcium imaging and brainstem electrophysiology. We found that CCK signaling at high concentrations (low-affinity binding) was potentiated in TRPV1-containing afferents and that TRPV1 itself mediated the enhanced CCK1R signaling. While competitive antagonism of TRPV1 failed to alter CCK1R signaling, TRPV1 pore blockade or genetic deletion (TRPV1 KO) significantly reduced the CCK response in cultured vagal afferents and eliminated its ability to increase spontaneous glutamate release in the NTS. Together, these results establish that TRPV1 mediates the low-affinity effects of CCK on vagal afferent activation and control of synaptic transmission in the brainstem. |
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| MeSH term(s) | Glutamic Acid/metabolism ; Solitary Nucleus ; Neurons, Afferent/metabolism ; Vagus Nerve ; Cholecystokinin/pharmacology ; Transient Receptor Potential Channels/metabolism |
| Chemical Substances | Glutamic Acid (3KX376GY7L) ; Cholecystokinin (9011-97-6) ; Transient Receptor Potential Channels |
| Language | English |
| Publishing date | 2023-12-04 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 392098-7 |
| ISSN | 1522-1563 ; 0363-6143 |
| ISSN (online) | 1522-1563 |
| ISSN | 0363-6143 |
| DOI | 10.1152/ajpcell.00409.2023 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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