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Article ; Online: Identification of SARS-CoV-2 in a Proficiency Testing Program.

Edson, Daniel C / Casey, Danielle L / Harmer, Susan E / Downes, Frances P

2020 Volume 154, Issue 4, Page(s) 475–478

Abstract: Objectives: At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention-developed reverse transcription polymerase chain reaction ... ...

Abstract	Objectives: At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention-developed reverse transcription polymerase chain reaction assay. The urgent and massive demand for testing prompted swift development of assays to detect SARS-CoV-2. The objective of this study was to assess the accuracy of these newly developed tests. Methods: The American Proficiency Institute sent 2 test samples to 346 clinical laboratories in order to assess the accuracy of SARS-CoV-2 assays. The positive sample, containing 5,175 viral copies/mL, was fully extractable with SARS-CoV-2 viral capsid protein and RNA. The negative sample, with 3,951 viral copies/mL, contained recombinant virus particles with sequences for targeting human RNAase P gene sequences. Results: Of the laboratories submitting results, 97.4% (302/310) correctly detected the virus when present and 98.3% (296/301) correctly indicated when the virus was not present. Among incorrect results reported in this proficiency challenge, 76.9% (10/13) were likely related to clerical error. This accounts for 1.6% (10/611) of all reported results. Conclusions: Overall performance in this SARS-CoV-2 RNA detection challenge was excellent, providing confidence in the results of these new molecular tests and assurance for the clinical and public health decisions based on these test results.
MeSH term(s)	Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Services/standards ; Clinical Laboratory Techniques/methods ; Clinical Laboratory Techniques/standards ; Coronavirus Infections/diagnosis ; Humans ; Laboratory Proficiency Testing ; Pandemics ; Pneumonia, Viral/diagnosis ; RNA, Viral/analysis ; RNA, Viral/isolation & purification ; SARS-CoV-2 ; United States
Chemical Substances	RNA, Viral
Keywords	covid19
Language	English
Publishing date	2020-08-10
Publishing country	England
Document type	Journal Article
ZDB-ID	2944-0
ISSN	1943-7722 ; 0002-9173
ISSN (online)	1943-7722
ISSN	0002-9173
DOI	10.1093/ajcp/aqaa128
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Article ; Online: Global proteome of the saprophytic strain Leptospira biflexa and comparative analysis with pathogenic strain Leptospira interrogans uncover new pathogenesis mechanisms.

Nascimento Filho, Edson G / Vieira, Mônica L / Dias, Meriellen / Mendes, Maria A / Sanchez, Fabio B / Setubal, João C / Heinemann, Marcos B / Souza, Gisele O / Pimenta, Daniel C / Nascimento, Ana L T O

Journal of proteomics

2024 Volume 297, Page(s) 105125

Abstract: Leptospira is a genus of bacteria that includes free-living saprophytic species found in water or soil, and pathogenic species, which are the etiologic agents of leptospirosis. Besides all the efforts, there are only a few proteins described as virulence ...

Abstract	Leptospira is a genus of bacteria that includes free-living saprophytic species found in water or soil, and pathogenic species, which are the etiologic agents of leptospirosis. Besides all the efforts, there are only a few proteins described as virulence factors in the pathogenic strain L. interrogans. This work aims to perform L. biflexa serovar Patoc1 strain Paris global proteome and to compare with the proteome database of pathogenic L. interrogans serovar Copenhageni strain Fiocruz L1-130. We identified a total of 2327 expressed proteins of L. biflexa by mass spectrometry. Using the Get Homologues software with the global proteome of L. biflexa and L. interrogans, we found orthologous proteins classified into conserved, low conserved, and specific proteins. Comparative bioinformatic analyses were performed to understand the biological functions of the proteins, subcellular localization, the presence of signal peptide, structural domains, and motifs using public softwares. These results lead to the selection of 182 low conserved within the saprophyte, and 176 specific proteins of L. interrogans. It is anticipated that these findings will indicate further studies to uncover virulence factors in the pathogenic strain. This work presents for the first time the global proteome of saprophytic strain L. biflexa serovar Patoc, strain Patoc1. SIGNIFICANCE: The comparative analysis established an array of specific proteins in pathogenic strain that will narrow down the identification of immune protective proteins that will help fight leptospirosis.
MeSH term(s)	Humans ; Leptospira interrogans ; Proteome/metabolism ; Leptospira ; Leptospirosis ; Virulence Factors/metabolism
Chemical Substances	Proteome ; Virulence Factors
Language	English
Publishing date	2024-02-14
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2024.105125
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Article ; Online: Changes in Patient Characteristics and Practice Outcomes of a Tele-Urgent Care Clinic Pre- and Post-COVID-19 Telehealth Policy Expansions.

Khairat, Saif / Yao, Yuxiao / Coleman, Cameron / McDaniel, Philip / Edson, Barbara / Shea, Christopher M

Perspectives in health information management

2022 Volume 19, Issue Spring, Page(s) 1k

Abstract: Introduction: The coronavirus 2019 pandemic (COVID-19) has resulted in major changes in lifestyle practices and healthcare delivery. The goal of this study was to examine changes in practice and service outcomes in a telehealth program before and after ... ...

Abstract	Introduction: The coronavirus 2019 pandemic (COVID-19) has resulted in major changes in lifestyle practices and healthcare delivery. The goal of this study was to examine changes in practice and service outcomes in a telehealth program before and after the federal and private telehealth policy expansion during the COVID-19 pandemic. These findings are particularly useful to understand what may be needed to overcome telehealth challenges in future disasters. Methods: We conducted a cross-sectional analysis of virtual visits through a statewide telehealth center embedded in a large academic healthcare system. Primary outcomes of this study were changes in telehealth visits pre- and post-policy expansions among at-risk populations. Results: A total of 2,132 telehealth visits were conducted: 1,530 (71.8 percent) patients were female, 1,561 (73.2 percent) were between the ages 18-50, 1,576 (74 percent) were uninsured, and 1,225 (57.5 percent) were from rural regions. The average number of telehealth visits per day increased from 14 to 33 visits post-expansion. A significant change in patient characteristics was found among senior, uninsured, and rural patients after the telehealth expansion.There was an 11 percent decrease in telehealth visits from very high vulnerability regions post-expansion compared to pre-expansion. There was a 15 percent decrease in visits resulting in prescription post-expansion (p-value<0.01). Conclusions: COVID-19 policy expansions expanded telehealth utilization among at-risk populations such as senior, uninsured, and rural patients while decompressing hospitals and emergency rooms and maintaining positive patient experiences. Further regulations are needed around virtual visits unintended consequences, software certification, and guidelines for workforce training.
MeSH term(s)	Adolescent ; Ambulatory Care Facilities ; COVID-19/prevention & control ; Cross-Sectional Studies ; Female ; Humans ; Male ; Pandemics/prevention & control ; Policy ; Telemedicine
Language	English
Publishing date	2022-03-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2422433-9
ISSN	1559-4122 ; 1559-4122
ISSN (online)	1559-4122
ISSN	1559-4122
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Article ; Online: Segmented solenoid RF coils for MRI of ex vivo brain samples at ultra-high field preclinical and clinical scanners

Daniel Papoti / Diego Szczupak / Luiz G.C. Santos / Khallil T. Chaim / Maria C.G. Otaduy / David J. Schaeffer / Edson L.G. Vidoto / Alberto Tannús / Afonso C. Silva

Journal of Magnetic Resonance Open, Vol 16, Iss , Pp 100103- (2023)

2023

Abstract: Magnetic resonance imaging (MRI) is a well-known and widespread imaging modality for neuroscience studies and the clinical diagnoses of neurological disorders, mainly due to its capability to visualize brain microstructures and quantify various ... ...

Abstract	Magnetic resonance imaging (MRI) is a well-known and widespread imaging modality for neuroscience studies and the clinical diagnoses of neurological disorders, mainly due to its capability to visualize brain microstructures and quantify various metabolites. Additionally, its noninvasive nature makes possible the correlation of high-resolution MRI from ex vivo brain samples with histology, supporting the study of neurodegenerative disorders such as Alzheimer's or Parkinson's disease. However, the quality and resolution of ex vivo MRI highly depend on the availability of specialized radiofrequency coils with maximized filling factors for the different sizes and shapes of the samples to be studied. For instance, small, dedicated radiofrequency (RF) coils are not always commercially available in ultrahigh field whole-body MRI scanners. Even for ultrahigh field preclinical scanners, specific RF coils for ex vivo MRI are expensive and not always available. Here, we describe the design and construction of two RF coils based on the solenoid geometry for ex vivo MRI of human brain tissues in a 7T whole-body scanner and for ex vivo MRI of marmoset brain samples in a 9.4T preclinical scanner. We designed the 7T solenoid RF coil to maximize the filling factor of human brain samples conditioned on cassettes for histology, while the 9.4T solenoid was constructed to accommodate marmoset brain samples conditioned in 50 ml centrifuge tubes. Both solenoid designs operate in transceiver mode. The measured B1+ maps show a high level of homogeneity in the imaging volume of interest, with a high signal-to-noise ratio over the imaging volume. High-resolution (80 µm in plane, 500 µm slice thickness) images of human brain samples were acquired with the 7T solenoid, while marmoset brain samples were acquired with an isotropic resolution of 60 µm using the 9.4T solenoid coil.
Keywords	RF coils ; Magnetic resonance imaging ; Ex vivo MRI ; Solenoid coils ; Medical physics. Medical radiology. Nuclear medicine ; R895-920 ; Physics ; QC1-999
Subject code	610
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Integrative assessment of cerebral blood regulation in COPD patients.

Corrêa, Daniel I / de-Lima-Oliveira, Marcelo / Nogueira, Ricardo C / Carvalho-Pinto, Regina M / Bor-Seng-Shu, Edson / Panerai, Ronney B / Carvalho, Celso R F / Salinet, Angela Sm

Respiratory physiology & neurobiology

2023 Volume 319, Page(s) 104166

Abstract: Cerebrovascular responses were compared between COPD and non-COPD participants. The association between COPD severity and cognitive function was also investigated. Cerebral blood velocity in the middle cerebral artery, blood pressure, and end-tidal ... ...

Abstract	Cerebrovascular responses were compared between COPD and non-COPD participants. The association between COPD severity and cognitive function was also investigated. Cerebral blood velocity in the middle cerebral artery, blood pressure, and end-tidal CO
MeSH term(s)	Humans ; Carbon Dioxide ; Cerebrovascular Circulation/physiology ; Neurovascular Coupling/physiology ; Middle Cerebral Artery ; Pulmonary Disease, Chronic Obstructive ; Blood Flow Velocity/physiology
Chemical Substances	Carbon Dioxide (142M471B3J)
Language	English
Publishing date	2023-09-26
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2077867-3
ISSN	1878-1519 ; 1569-9048
ISSN (online)	1878-1519
ISSN	1569-9048
DOI	10.1016/j.resp.2023.104166
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Article: Identification of SARS-CoV-2 in a Proficiency Testing Program

Edson, Daniel C / Casey, Danielle L / Harmer, Susan E / Downes, Frances P

Am J Clin Pathol

Abstract: OBJECTIVES: At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention-developed reverse transcription polymerase chain reaction ... ...

Abstract	OBJECTIVES: At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention-developed reverse transcription polymerase chain reaction assay. The urgent and massive demand for testing prompted swift development of assays to detect SARS-CoV-2. The objective of this study was to assess the accuracy of these newly developed tests. METHODS: The American Proficiency Institute sent 2 test samples to 346 clinical laboratories in order to assess the accuracy of SARS-CoV-2 assays. The positive sample, containing 5,175 viral copies/mL, was fully extractable with SARS-CoV-2 viral capsid protein and RNA. The negative sample, with 3,951 viral copies/mL, contained recombinant virus particles with sequences for targeting human RNAase P gene sequences. RESULTS: Of the laboratories submitting results, 97.4% (302/310) correctly detected the virus when present and 98.3% (296/301) correctly indicated when the virus was not present. Among incorrect results reported in this proficiency challenge, 76.9% (10/13) were likely related to clerical error. This accounts for 1.6% (10/611) of all reported results. CONCLUSIONS: Overall performance in this SARS-CoV-2 RNA detection challenge was excellent, providing confidence in the results of these new molecular tests and assurance for the clinical and public health decisions based on these test results.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #705105
Database	COVID19

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Article ; Online: Identification of SARS-CoV-2 in a Proficiency Testing Program

Edson, Daniel C / Casey, Danielle L / Harmer, Susan E / Downes, Frances P

American Journal of Clinical Pathology

2020 Volume 154, Issue 4, Page(s) 475–478

Abstract: Abstract Objectives At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention–developed reverse transcription polymerase chain ... ...

Abstract	Abstract Objectives At the onset of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in the United States, testing was limited to the Centers for Disease Control and Prevention–developed reverse transcription polymerase chain reaction assay. The urgent and massive demand for testing prompted swift development of assays to detect SARS-CoV-2. The objective of this study was to assess the accuracy of these newly developed tests. Methods The American Proficiency Institute sent 2 test samples to 346 clinical laboratories in order to assess the accuracy of SARS-CoV-2 assays. The positive sample, containing 5,175 viral copies/mL, was fully extractable with SARS-CoV-2 viral capsid protein and RNA. The negative sample, with 3,951 viral copies/mL, contained recombinant virus particles with sequences for targeting human RNAase P gene sequences. Results Of the laboratories submitting results, 97.4% (302/310) correctly detected the virus when present and 98.3% (296/301) correctly indicated when the virus was not present. Among incorrect results reported in this proficiency challenge, 76.9% (10/13) were likely related to clerical error. This accounts for 1.6% (10/611) of all reported results. Conclusions Overall performance in this SARS-CoV-2 RNA detection challenge was excellent, providing confidence in the results of these new molecular tests and assurance for the clinical and public health decisions based on these test results.
Keywords	General Medicine ; covid19
Language	English
Publisher	Oxford University Press (OUP)
Publishing country	uk
Document type	Article ; Online
ZDB-ID	2944-0
ISSN	1943-7722 ; 0002-9173
ISSN (online)	1943-7722
ISSN	0002-9173
DOI	10.1093/ajcp/aqaa128
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Proficiency testing in the lab as an investigative tool.

Edson, Daniel C / Stombler, Robin E

MLO: medical laboratory observer

2011 Volume 43, Issue 8, Page(s) 32

MeSH term(s)	Laboratories/standards ; Laboratory Proficiency Testing/methods ; Problem Solving ; Quality Assurance, Health Care ; United States
Language	English
Publishing date	2011-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	603205-9
ISSN	0580-7247
ISSN	0580-7247
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Article ; Online: Characterization of Cuban and Brazilian natural zeolites by photoacoustic spectroscopy and electron paramagnetic resonance

ARLEM LARA-BERNAL / MARCELO G. DA SILVA / LEONARDO MOTA / ERNESTO MARÍN / THALLIS C. CORDEIRO / EDSON C. DA SILVA / DANIEL A. VIANA / HELION VARGAS / JOSÉ CARLOS POLIDORO / MARISA BEZERRA DE MELLO-MONTE

Anais da Academia Brasileira de Ciências, Vol 94, Iss

2022 Volume 1

Abstract: ... 1/813 cm − 1 and C- BRA/C- CUB = 3655 cm − 1/2496 cm − 1) parameters were assessed as well ...

Abstract	Abstract This report describes the photoacoustic and electron paramagnetic resonance investigations of Brazilian and Cuban zeolites. Photoacoustic optical absorption measurements indicate the presence of iron (Fe 3 +) ions with their respective transition bands for both zeolites. Two species of manganese (Mn 2 + and Mn 3 +) were identified in the Cuban sample and the electronic transitions assigned. Iron and manganese ions were confirmed through nonradiative relaxation ( τ) and characteristic diffusion ( τ β) times evaluation, whose values were found to be τ BRA = 5.40 ms, τ CUB = 4.60 ms, τ β BRA = 387 μs and τ β CUB = 305 μs. Crystal field (Dq- BRA/Dq- CUB = 1048 cm − 1/945 cm − 1) plus Racah (B- BRA/B- CUB = 457 cm − 1/813 cm − 1 and C- BRA/C- CUB = 3655 cm − 1/2496 cm − 1) parameters were assessed as well. Paramagnetic resonance corroborated Fe 3 + ions present in the Brazilian zeolite occupying sites showing axial and/or rhombic symmetry distortions. For the Cuban sample, results reveal the characteristic hyperfine sextet lines of Mn 2 + overlapping the Fe 3 + line. Values of Landé factor and isotropic hyperfine splitting constant were found to be 2.0 and 9.7 mT, respectively. This tells us that the Mn 2 + lies in octahedral symmetry probably replacing calcium ions and point towards an ionic bonding character of the Mn 2 + and its surroundings.
Keywords	Characteristic diffusion time ; natural zeolites ; nonradiative relaxation time ; phase resolved method ; Racah parameters ; Science ; Q
Subject code	541
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Academia Brasileira de Ciências
Document type	Article ; Online
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Article ; Online: Provision of a protein-rich supplement for grazing suckling female beef calves to improve productive performance and metabolic response

Deilen S Moreno / Román M Ortega / David C Marquez / Thiago R Moreira / Edson J dos Santos / Daniel M de Almeida / Mário F Paulino / Luciana N Rennó / Edenio Detmann

Animal Bioscience, Vol 35, Iss 8, Pp 1174-

2022 Volume 1183

Abstract: Objective This study was conducted to evaluate the effects of the provision of a protein-rich supplement on productive performance, and metabolic profile on grazing suckling female beef calves in tropical conditions during 150 d of experimentation. ... ...

Abstract	Objective This study was conducted to evaluate the effects of the provision of a protein-rich supplement on productive performance, and metabolic profile on grazing suckling female beef calves in tropical conditions during 150 d of experimentation. Methods Fifty-six Nellore suckling female calves, and their respective dams were distributed in a completely randomised design and made to undergo two treatments as follows: UNS (without supplementation), and SUP (supplementation with 5 g/kg body weight [BW] of a protein supplement). Throughout the experiment, animal performance and metabolic profile were evaluated. Also, ureagenesis and gluconeogenesis were assessed for gene expression. Results SUP female calves showed a higher voluntary intake (p≤0.03) of the diet components evaluated, digestibility of organic matter (p≤0.02) and microbial nitrogen production (MICN; p≤0.02) compared to UNS female calves. In its turn, serum urea nitrogen (p≤0.01) and insulin-like growth factor-1 (p≤0.03) levels and ureagenesis (p≤0.04) increased in SUP female calves compared to UNS female calves. Blood glucose and triglyceride levels were not affected by supplementation. The average daily gain (ADG) from SUP female calves was higher (p≤0.02) compared with UNS female calves. However, supplementation did not affect the body measures of the animals. Conclusion In summary, provision of a protein-rich supplement improves the intake and nutrients digestibility, ADG and final BW and increases metabolic indicators of the protein status in grazing suckling female beef calves in tropical conditions.
Keywords	biopsy ; cattle ; gene expression ; gluconeogenesis ; ruminant feeding ; Zoology ; QL1-991
Subject code	630
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	Asian-Australasian Association of Animal Production Societies
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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