Article ; Online: Identification of SER-PRO-CYS Peptide in Blood Serum of Multiple Sclerosis Patients.
2016 Volume 23, Issue 9, Page(s) 808–811
Abstract: Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the ... ...
| Abstract | Monitoring of multiple sclerosis (MS) requires additional molecular markers. Recently, we used original TCA-precipitation/extraction approach in combination with MALDI TOF/TOF mass-spectrometry and identified earlier unknown 48 kDa form of the unconventional myosin IC isoform b (Myo1C) in blood serum of the MS patients. Further examination of TCA-extracted fraction of blood serum of these patients by means of thin-layer chromatography and HPLC gel-filtration allowed detecting 300-500 Da peptides. MALDI TOF/TOF massspectrometry of these peptides showed that they contain Ser-Pro-Cys amino acid sequence. We discussed potential mechanisms of a release of these peptides that were earlier unknown in blood serum of the MS patients. |
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| MeSH term(s) | Adult ; Amino Acid Sequence ; Biomarkers/blood ; Cysteine/metabolism ; Female ; Humans ; Male ; Middle Aged ; Multiple Sclerosis/metabolism ; Peptides/blood ; Peptides/chemistry ; Peptides/isolation & purification ; Proline/metabolism ; Serine/metabolism ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tandem Mass Spectrometry ; Young Adult |
| Chemical Substances | Biomarkers ; Peptides ; Serine (452VLY9402) ; Proline (9DLQ4CIU6V) ; Cysteine (K848JZ4886) |
| Language | English |
| Publishing date | 2016-06-16 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 1280776-x |
| ISSN | 1875-5305 ; 0929-8665 |
| ISSN (online) | 1875-5305 |
| ISSN | 0929-8665 |
| DOI | 10.2174/0929866523666160622215628 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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