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Article ; Online: Mechanical Stimulation of Anti-Inflammatory and Antioxidant Hydrogels for Rapid Re-Epithelialization.

Yang, Wei / Zhong, Wenwen / Yan, Shengtao / Wang, Shuting / Xuan, Chengkai / Zheng, Ke / Qiu, Jianguang / Shi, Xuetao

Advanced materials (Deerfield Beach, Fla.)

2024 , Page(s) e2312740

Abstract: The epithelium, an essential barrier to protect organisms against infection, exists in many organs. However, rapid re-epithelialization to restore tissue integrity and function in an adverse environment is challenging. In this work, a long-term anti- ... ...

Abstract	The epithelium, an essential barrier to protect organisms against infection, exists in many organs. However, rapid re-epithelialization to restore tissue integrity and function in an adverse environment is challenging. In this work, a long-term anti-inflammatory and antioxidant hydrogel with mechanical stimulation for rapid re-epithelialization, mainly composed of the small molecule thioctic acid, biocompatible glycine, and γ-Fe
Language	English
Publishing date	2024-01-25
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1474949-X
ISSN	1521-4095 ; 0935-9648
ISSN (online)	1521-4095
ISSN	0935-9648
DOI	10.1002/adma.202312740
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Article ; Online: Poly-L-lactic acid/gelatin electrospun membrane-loaded bone marrow-derived mesenchymal stem cells attenuate erectile dysfunction caused by cavernous nerve injury.

Hu, Daoyuan / Liu, Chang / Ge, Yunlong / Ye, Lei / Guo, Qiang / Xi, Yuhang / Zhu, Wenliang / Wang, Dejuan / Xu, Tao / Qiu, Jianguang

International journal of biological macromolecules

2024 Volume 265, Issue Pt 2, Page(s) 131099

Abstract: Radical prostatectomy (RP) can cause neurogenic erectile dysfunction (ED), which negatively affects the quality of life of patients with prostate cancer. Currently, there is a dearth of effective therapeutic strategies. Although stem cell therapy is ... ...

Abstract	Radical prostatectomy (RP) can cause neurogenic erectile dysfunction (ED), which negatively affects the quality of life of patients with prostate cancer. Currently, there is a dearth of effective therapeutic strategies. Although stem cell therapy is promising, direct cell transplantation to injured cavernous nerves is constrained by poor cell colonization. In this study, poly-L-lactic acid (PLLA)/gelatin electrospun membranes (PGEM) were fabricated to load bone marrow-derived mesenchymal stem cells (BM-MSCs) as a patch to be placed on injured nerves to alleviate ED. This study aimed to establish a promising and innovative approach to mitigate neurogenic ED post-RP and lay the foundation for modifying surgical procedures. Electrospinning and molecular biotechnology were performed in vitro and in vivo, respectively. It was observed that PGEM enhanced the performance of BM-MSCs and Schwann cells due to their excellent mechanical properties and biocompatibility. The transplanted PGEM and loaded BM-MSCs synergistically improved bilateral cavernous nerve injury-related ED and the corresponding histopathological changes. Nevertheless, transplantation of BM-MSCs alone has been verified to be ineffective. Overall, PGEM can serve as an ideal carrier to supply a more suitable survival environment for BM-MSCs and Schwann cells, thereby promoting the recovery of injured cavernous nerves and erectile function.
MeSH term(s)	Male ; Rats ; Animals ; Humans ; Erectile Dysfunction/etiology ; Erectile Dysfunction/therapy ; Gelatin/metabolism ; Penis/innervation ; Penis/pathology ; Bone Marrow/pathology ; Quality of Life ; Rats, Sprague-Dawley ; Disease Models, Animal ; Mesenchymal Stem Cells/metabolism ; Polyesters
Chemical Substances	poly(lactide) (459TN2L5F5) ; Gelatin (9000-70-8) ; Polyesters
Language	English
Publishing date	2024-03-22
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.131099
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Article: Analysis of a Long Non-coding RNA associated Signature to Predict Survival in Patients with Bladder Cancer.

Zhong, Wenwen / Qu, Hu / Yao, Bing / Wang, Dejuan / Qiu, Jianguang

Cureus

2022 Volume 14, Issue 5, Page(s) e24818

Abstract: Background: The study aimed to find a potential long non-coding RNA (lncRNA) model related to survival in bladder cancer by analyzing data in The Cancer Genome Atlas (TCGA).: Methods: We downloaded the gene expression data from the TCGA and analyzed ... ...

Abstract	Background: The study aimed to find a potential long non-coding RNA (lncRNA) model related to survival in bladder cancer by analyzing data in The Cancer Genome Atlas (TCGA). Methods: We downloaded the gene expression data from the TCGA and analyzed the differentially expressed lncRNAs (DELs) between tumor and normal tissues. Patients were divided into training and testing groups, and a prognostic risk score model with lncRNAs was constructed by using data in the training group using multivariate Cox and lasso regression analysis. We divided patients into high-and low-risk groups according to the median value in the lncRNA signature model. Survival and receiver operating characteristic (ROC) curves were visualized in both groups. Further, we validated the model in the testing group. Results: We screened 169 DELs for bladder cancer. The univariate Cox regression analysis showed that 13 lncRNAs were associated with prognosis with a Conclusions: The study illustrated a significant lncRNA signature and indicated the risk score Cox model could be an important biomarker to predict the prognosis of bladder cancer.
Language	English
Publishing date	2022-05-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.24818
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Article ; Online: Laparoscopic assisted trans-scrotal orchiopexy versus traditional orchiopexy for inguinal cryptorchidism: a retrospective study based on 154 patients.

Guo, Qiang / Zhang, Yifei / Lai, Huajian / Zhong, WenWen / Qiu, Jianguang / Wang, Dejuan

BMC urology

2023 Volume 23, Issue 1, Page(s) 84

Abstract: Background: The purpose of this study was to investigate the clinical effect of laparoscopic assisted trans-scrotal orchiopexy versus traditional orchiopexy for inguinal cryptorchidism.: Methods: A retrospective analysis of cryptorchidism patients ... ...

Abstract	Background: The purpose of this study was to investigate the clinical effect of laparoscopic assisted trans-scrotal orchiopexy versus traditional orchiopexy for inguinal cryptorchidism. Methods: A retrospective analysis of cryptorchidism patients who were admitted to our hospital from July 2018 to July 2021. The patients were divided into the laparoscopic assisted trans-scrotal surgery group (n = 76) and the traditional surgery group (n = 78) according to the surgical method. Results: All patients were successfully operated. There was no significant difference in operation time between the laparoscopic assisted trans-scrotal group and the traditional group (P>0.05). Although there was no significant difference in the postoperative hospital stay between the two groups, the time of postoperative hospital stay of the laparoscopic assisted trans-scrotal surgery group was lower than that in the traditional surgery group (P = 0.062). Additionally, there was no significant difference in discharge rate on the first day after surgery between the two groups, but the discharge rate on the first day after surgery was more than 90% in both groups. In terms of postoperative complications, there were no cases of testicular retraction, testicular atrophy, inguinal hernia, or hydrocele that occurred in both groups. There was no significant difference in the incidence of scrotal hematoma between the two groups(P>0.05). Although there was no significant difference in the incidence of poor wound healing between the two groups(P>0.05), the incidence in the laparoscopic assisted trans-scrotal surgery group was lower than that in the traditional surgery group (2.6% vs. 6.4%). Conclusion: Laparoscopic assisted trans-scrotal surgery is as safe and effective method as traditional surgery for patients with inguinal cryptorchidism, and could also provide a good appearance.
MeSH term(s)	Male ; Humans ; Infant ; Cryptorchidism/surgery ; Orchiopexy/methods ; Retrospective Studies ; Scrotum/surgery ; Laparoscopy ; Treatment Outcome
Language	English
Publishing date	2023-05-06
Publishing country	England
Document type	Journal Article
ZDB-ID	2059857-9
ISSN	1471-2490 ; 1471-2490
ISSN (online)	1471-2490
ISSN	1471-2490
DOI	10.1186/s12894-023-01244-3
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Article ; Online: Hydrolytic Metabolism of Withangulatin A Mediated by Serum Albumin Instead of Common Esterases in Plasma.

Zhuang, Yu / Wang, Yuxiao / Li, Ning / Meng, Haitao / Li, Zhiyu / Luo, Jianguang / Qiu, Zhixia

European journal of drug metabolism and pharmacokinetics

2023 Volume 48, Issue 4, Page(s) 363–376

Abstract: Background and objectives: The oral bioavailability of withangulatin A (WA) is low and may undergo first-pass metabolism because of the presence of two esters bonds. This study aimed to identify the hydrolysis behavior and mechanism of WA, thus ... ...

Abstract	Background and objectives: The oral bioavailability of withangulatin A (WA) is low and may undergo first-pass metabolism because of the presence of two esters bonds. This study aimed to identify the hydrolysis behavior and mechanism of WA, thus enriching its structure-pharmacokinetic relationship. Methods: The in vivo pharmacokinetic studies of WA in rats were first investigated, followed by in vitro assays including metabolic stability, phenotyping identification and metabolic kinetics assays. After screening out the responsible enzymes with higher catalytic capacity, molecular docking study was performed to demonstrate the interaction mode between WA and metabolic enzymes. Then, metabolites in human serum albumin (HSA) were identified by LC-TOF-MS/MS. Results: In rats, the oral bioavailability of WA was only 2.83%. In vitro, WA was hydrolyzed in both rat and human plasma and could not be inhibited by selective esterase inhibitors. Physiologic concentration of HSA not recombinant human carboxylesterases (rhCES) could significantly hydrolyze WA, and it had a similar hydrolytic capacity with human plasma to WA. Furthermore, WA could stably bind to HSA by forming hydrogen bonds with Lys199 and Arg410, accompanied by the metabolic reaction of the lactone ring opening. Conclusion: The study showed that WA underwent obvious hydrolysis in rat and human plasma, which implied a strong first-pass effect. Serum albumin rather than common esterases primarily contributed to the hydrolytic metabolism of WA in plasma.
MeSH term(s)	Rats ; Humans ; Animals ; Esterases/metabolism ; Hydrolysis ; Serum Albumin/metabolism ; Molecular Docking Simulation ; Tandem Mass Spectrometry ; Serum Albumin, Human
Chemical Substances	Esterases (EC 3.1.-) ; Serum Albumin ; withangulatin A (120824-03-5) ; Serum Albumin, Human (ZIF514RVZR)
Language	English
Publishing date	2023-06-21
Publishing country	France
Document type	Journal Article
ZDB-ID	196729-0
ISSN	2107-0180 ; 0398-7639 ; 0378-7966
ISSN (online)	2107-0180
ISSN	0398-7639 ; 0378-7966
DOI	10.1007/s13318-023-00834-8
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Article ; Online: Diversified responses of vegetation carbon uptake to urbanization

Xueliang Zhang / Dai Qiu / Yichun Xie / Jianguang Tu / Hai Lan / Xiaolei Li / Zongyao Sha

Frontiers in Ecology and Evolution, Vol

a national-scale analysis

2023 Volume 11

Abstract: IntroductionUrbanization converts vegetated lands into impervious surfaces and often degrades vegetation carbon sequestration in urban ecosystems. At the same time, the impact on urban vegetation growth from urban expansion could be spatially diverse ... ...

Abstract	IntroductionUrbanization converts vegetated lands into impervious surfaces and often degrades vegetation carbon sequestration in urban ecosystems. At the same time, the impact on urban vegetation growth from urban expansion could be spatially diverse given different natural environments and urban management practices.MethodsHere we applied time-series remotely sensed images and analyzed the urban growth for all the prefecture-level cities across China during 2001–2019, and compared the impact of urbanization on vegetation carbon uptake proxied by MODIS (MOD17A2H) net primary productivity (NPP) on Google Earth Engine platform.ResultsThe result indicated that at the national scale, the carbon uptake flux in urban areas was only 19% compared to that in the nonurban vegetated counterparts. The total urban area expanded by 22% and the vegetation carbon uptake in the newly urbanized zones was averagely reduced by 16% during the period, but with high spatio-temporal heterogeneity among cities and with exceptions demonstrating even improved NPP, highlighting diversified responses of vegetation carbon sequestration to urban sprawl. The changes of vegetation carbon sequestration in response to urbanization were found to be spatially clustered.DiscussionWe conclude that urban land management strategies unique to cities may attribute to the diversified responses of vegetation carbon capture to urbanization.
Keywords	urbanization ; vegetation productivity ; remote sensing ; carbon sink ; urban planning ; urban ecosystems ; Evolution ; QH359-425 ; Ecology ; QH540-549.5
Subject code	710
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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Article ; Online: Circ_0000033 up-regulates NUAK2 by sequestering miR-378a-3p to promote breast tumorigenesis.

Dai, Yijun / Shi, Wenjian / Qiu, Yanru / Xu, Tianwen / Lin, Jianguang / Su, Yunxia

Environmental and molecular mutagenesis

2023 Volume 64, Issue 6, Page(s) 359–370

Abstract: Circular RNAs (circRNAs), including circ_0000033, were shown to be abnormally expressed in breast cancer (BC) and play an important regulatory function in the development of this cancer. This study aimed to investigate the action and mechanism of ... ...

Abstract	Circular RNAs (circRNAs), including circ_0000033, were shown to be abnormally expressed in breast cancer (BC) and play an important regulatory function in the development of this cancer. This study aimed to investigate the action and mechanism of circ_0000033 in BC carcinogenesis. Specifically, levels of genes and proteins were analyzed using quantitative real-time PCR (qRT-PCR) and western blotting. Circ_0000033 was highly expressed in BC tissues and cells. Properties of cells with modified expression of circ_0000033 were characterized using an in vitro colony formation assay, EdU assay, flow cytometry, caspase-3 activity analysis, transwell assay, and tube formation assay, respectively. Functionally, knockdown of circ_0000033 suppressed BC cell proliferation, migration, invasion, angiogenesis, and induced apoptosis and cell cycle arrest in vitro. An in vivo experiment was conducted using a murine xenograft model and showed circ_0000033 silencing also impeded the growth of BC in nude mice. The binding between miR-378a-3p and circ_0000033 or NUAK2 (NUAK Family Kinase 2) was validated using a dual-luciferase reporter assay. Circ_0000033 sequestered miR-378a-3p and resulted in NUAK2 release, indicating a circ_0000033/miR-378a-3p/NUAK2 regulatory network operates in BC cells. Circ_0000033 down-regulation in BC cells was accompanied by decreased NUAK2 and increased miR-378a-3p expression. Moreover, the anticancer effects mediated by circ_0000033 knockdown were abolished by miR-378a-3p inhibition or NUAK2 overexpression in BC cells. Overall, circ_0000033 up-regulates NUAK2 through sequestration miR-378a-3p, which promoted breast tumorigenesis, suggesting circ_0000033 is a promising therapeutic target for BC treatment.
MeSH term(s)	Humans ; Animals ; Mice ; Female ; Mice, Nude ; Cell Transformation, Neoplastic ; Carcinogenesis/genetics ; Breast Neoplasms/genetics ; MicroRNAs/genetics ; Cell Proliferation/genetics ; Cell Line, Tumor ; Protein Serine-Threonine Kinases
Chemical Substances	MicroRNAs ; NUAK2 protein, human (EC 2.7.11.1) ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2023-07-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639145-x
ISSN	1098-2280 ; 0893-6692
ISSN (online)	1098-2280
ISSN	0893-6692
DOI	10.1002/em.22558
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Article ; Online: The miR-199a-5p/PD-L1 axis regulates cell proliferation, migration and invasion in follicular thyroid carcinoma.

Lin, Jianguang / Qiu, Yanru / Zheng, Xueqin / Dai, Yijun / Xu, Tianwen

BMC cancer

2022 Volume 22, Issue 1, Page(s) 756

Abstract: Background: Follicular thyroid carcinoma (FTC) is the second most common cancer of the thyroid and easily develops into distant metastasis. PD-L1 is known to be associated with the carcinogenesis and progression of thyroid carcinoma. Our study aimed to ... ...

Abstract	Background: Follicular thyroid carcinoma (FTC) is the second most common cancer of the thyroid and easily develops into distant metastasis. PD-L1 is known to be associated with the carcinogenesis and progression of thyroid carcinoma. Our study aimed to investigate the biological functions of PD-L1 and to identify miRNAs that were responsible for modulating the activity of PD-L1. Methods: A total of 72 patients with FTC at The Second Affiliated Hospital of Fujian Medical University were enrolled in this retrospective study. Immunohistochemical (IHC) assay was used to measure PD-L1 expression in FTC. The association between PD-L1 expression and clinicopathologic characteristics was evaluated. Bioinformatics analysis, RT-qPCR and western blotting were used to examine the relationships between miR-199a-5p, PD-L1 and Claudin-1. Cell proliferation, migration and invasion were evaluated by using CCK8 and Transwell migration and invasion assays. Target prediction and luciferase reporter assays were performed to verify the binding between miR-199a-5p and PD-L1. Rescue assay was performed to confirm whether PD-L1 downregulation abolished the inhibitory effect of miR-199a-5p. Results: Among 72 pairs of tumor and normal specimens, the proportion of PD-L1 positive samples was higher in FTC tissues than in normal tissues. The results of ESTIMATE and CIBERSORT illustrated that there was a positive correlation between PD-L1 expression and immune infiltration, especially regulatory T cells and M1 macrophages. Prediction of immunotherapy revealed that patients with high PD-L1 expression might benefit from immune checkpoint inhibitors. Transwell migration and invasion assays showed that PD-L1 downregulation in FTC cells could significantly inhibit cell migration and invasion. The bioinformatics analysis and luciferase activity results indicated that PD-L1 was a potential target of miR-199a-5p. Knockdown of PD-L1 reversed the miR-199a-5p inhibitor mediated promotion effect. In addition, we found that PD-L1 expression was positively correlated with Claudin-1 expression and that miR-199a-5p affected the progression of FTC cells through the negative regulation of PD-L1 and Claudin-1. Conclusions: Our study revealed that PD-L1 expression was elevated in FTC and was closely associated with tumor aggressiveness and progression. MiR-199a-5p has a functional role in the progression and metastasis of FTC by regulating PD-L1 and Claudin-1 expression.
MeSH term(s)	Adenocarcinoma, Follicular/genetics ; B7-H1 Antigen/genetics ; B7-H1 Antigen/metabolism ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics ; Claudin-1/metabolism ; Gene Expression Regulation, Neoplastic ; Humans ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Retrospective Studies ; Thyroid Neoplasms/pathology
Chemical Substances	B7-H1 Antigen ; Claudin-1 ; MicroRNAs
Language	English
Publishing date	2022-07-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2041352-X
ISSN	1471-2407 ; 1471-2407
ISSN (online)	1471-2407
ISSN	1471-2407
DOI	10.1186/s12885-022-09838-0
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Article ; Online: Retrospective analysis of clinical characteristics of and treatment strategies for patients with long-acting anticoagulant rodenticide poisoning.

Guodong, Lin / Jieyi, Wu / Xiaobo, Peng / Xiaoxia, Lu / Zhongying, Liu / Zhiguo, Pan / Zewu, Qiu / Jianguang, Dong

Basic & clinical pharmacology & toxicology

2022 Volume 131, Issue 1, Page(s) 74–82

Abstract: Objective: Long-acting anticoagulant rodenticide (LAAR) poisoning remains a serious public health problem. The aim of this study was to explore the clinical characteristics of different LAARs and a method of making a decision on the VK1 treatment course ...

Abstract	Objective: Long-acting anticoagulant rodenticide (LAAR) poisoning remains a serious public health problem. The aim of this study was to explore the clinical characteristics of different LAARs and a method of making a decision on the VK1 treatment course and the time to stop VK1 treatment safely. Method: This retrospective study compared the clinical characteristics of two LAARs poisoning patients and used multivariate regression method to explore the relationship between blood LAAR concentration and vitamin K1 dose/treatment time. Result: A total of 115 patients with LAAR poisoning were included in this study after screening. Of these, 50 patients attempted to commit suicide. The median LAAR concentration of the patients at admission was 409 (157-1174) ng/mL, and the VK1 treatment duration was 14 (8-34) days. The total VK1 treatment time in patients with LAAR poisoning was positively correlated with admission LAAR concentration. During the maintenance treatment period, the VK1 dosage was positively correlated with blood LAAR concentration. Conclusion: Low dose of VK1 during the maintenance period is indicative of the blood LAAR concentration being relatively low. This provides a basis for judging the LAAR content in the body during the maintenance treatment period.
MeSH term(s)	Anticoagulants ; Humans ; Poisoning/diagnosis ; Poisoning/drug therapy ; Retrospective Studies ; Rodenticides ; Vitamin K 1
Chemical Substances	Anticoagulants ; Rodenticides ; Vitamin K 1 (84-80-0)
Language	English
Publishing date	2022-05-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2134679-3
ISSN	1742-7843 ; 1742-7835
ISSN (online)	1742-7843
ISSN	1742-7835
DOI	10.1111/bcpt.13734
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Article ; Online: Loperamide induces protective autophagy and apoptosis through the ROS/JNK signaling pathway in bladder cancer.

Wu, Jianjian / Guo, Qiang / Li, Juntao / Yuan, Hao / Xiao, Chutian / Qiu, Jianguang / Wu, Qiong / Wang, Dejuan

Biochemical pharmacology

2023 Volume 218, Page(s) 115870

Abstract: Bladder cancer is one of the most common carcinomas in the human urinary system worldwide. Loperamide, known as an antidiarrheal drug, exerts anti-tumor activities against various cancers. However, the effect of loperamide on bladder cancer cells remains ...

Abstract	Bladder cancer is one of the most common carcinomas in the human urinary system worldwide. Loperamide, known as an antidiarrheal drug, exerts anti-tumor activities against various cancers. However, the effect of loperamide on bladder cancer cells remains unclear. Our study aimed to investigate the effect of loperamide on bladder cancer and explore the underlying mechanisms. We found that loperamide suppressed the proliferation of 5637 and T24 cells in a dose-dependent manner. Loperamide treatment showed both pro-apoptotic and pro-autophagic effects on bladder cancer cells. Moreover, it was revealed that loperamide induced reactive oxygen species (ROS) accumulation, leading to the activation of c-Jun N-terminal kinase (JNK) signaling pathway. Notably, ROS scavenger N-acetyl-L-cysteine (NAC) and JNK inhibitor SP600125 effectively attenuated the induction of autophagy and apoptosis triggered by loperamide. Finally, blocking autophagy with CQ could significantly enhance the anti-cancer effect of loperamide both in vitro and in vivo. Overall, these findings demonstrated that loperamide induced autophagy and apoptosis through the ROS-mediated JNK pathway in bladder cancer cells. Our results suggest that the strategy of combining loperamide with autophagy inhibitor CQ may provide a therapeutic option for the treatment of bladder cancer.
MeSH term(s)	Humans ; MAP Kinase Signaling System ; Loperamide/pharmacology ; Reactive Oxygen Species/metabolism ; Cell Line, Tumor ; Apoptosis ; Urinary Bladder Neoplasms/drug therapy ; Autophagy ; JNK Mitogen-Activated Protein Kinases/metabolism
Chemical Substances	Loperamide (6X9OC3H4II) ; Reactive Oxygen Species ; JNK Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2023-10-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208787-x
ISSN	1873-2968 ; 0006-2952
ISSN (online)	1873-2968
ISSN	0006-2952
DOI	10.1016/j.bcp.2023.115870
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