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  1. Article: Nano-flow cytometry unveils mitochondrial permeability transition process and multi-pathway cell death induction for cancer therapy.

    Su, Liyun / Xu, Jingyi / Lu, Cheng / Gao, Kaimin / Hu, Yunyun / Xue, Chengfeng / Yan, Xiaomei

    Cell death discovery

    2024  Volume 10, Issue 1, Page(s) 176

    Abstract: Mitochondrial permeability transition (mPT)-mediated mitochondrial dysfunction plays a pivotal role in various human diseases. However, the intricate details of its mechanisms and the sequence of events remain elusive, primarily due to the interference ... ...

    Abstract Mitochondrial permeability transition (mPT)-mediated mitochondrial dysfunction plays a pivotal role in various human diseases. However, the intricate details of its mechanisms and the sequence of events remain elusive, primarily due to the interference caused by Bax/Bak-induced mitochondrial outer membrane permeabilization (MOMP). To address these, we have developed a methodology that utilizes nano-flow cytometry (nFCM) to quantitatively analyze the opening of mitochondrial permeability transition pore (mPTP), dissipation of mitochondrial membrane potential (
    Language English
    Publishing date 2024-04-15
    Publishing country United States
    Document type Journal Article
    ISSN 2058-7716
    ISSN 2058-7716
    DOI 10.1038/s41420-024-01947-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Moderate Hyperglycemia-Preventive Effect and Mechanism of Action of

    Lu, Kaimin / He, Yufei / Wu, Chuanfang / Bao, Jinku

    Nutrients

    2022  Volume 14, Issue 21

    Abstract: Periplaneta ... ...

    Abstract Periplaneta americana
    MeSH term(s) Mice ; Animals ; Streptozocin ; Diabetes Mellitus, Experimental/metabolism ; Periplaneta/metabolism ; Blood Glucose/metabolism ; Phosphatidylinositol 3-Kinases ; Hyperglycemia/prevention & control ; Oligosaccharides
    Chemical Substances Streptozocin (5W494URQ81) ; Blood Glucose ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Oligosaccharides
    Language English
    Publishing date 2022-11-02
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518386-2
    ISSN 2072-6643 ; 2072-6643
    ISSN (online) 2072-6643
    ISSN 2072-6643
    DOI 10.3390/nu14214620
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Mitochondrial Esterase Activity Measured at the Single Organelle Level by Nano-flow Cytometry.

    Su, Liyun / Gao, Kaimin / Tian, Ye / Xiao, Xu / Lu, Cheng / Xu, Jingyi / Yan, Xiaomei

    Analytical chemistry

    2024  Volume 96, Issue 2, Page(s) 810–820

    Abstract: Monitoring mitochondrial esterase activity is crucial not only for investigating mitochondrial metabolism but also for assessing the effectiveness of mitochondrial-targeting prodrugs. However, accurately detecting esterase activity within mitochondria ... ...

    Abstract Monitoring mitochondrial esterase activity is crucial not only for investigating mitochondrial metabolism but also for assessing the effectiveness of mitochondrial-targeting prodrugs. However, accurately detecting esterase activity within mitochondria poses challenges due to its ubiquitous presence in cells and the uncontrolled localization of fluorogenic probes. To overcome this hurdle and reveal variations among different mitochondria, we isolated mitochondria and preserved their activity and functionality in a buffered environment. Subsequently, we utilized a laboratory-built nano-flow cytometer in conjunction with an esterase-responsive calcein-AM fluorescent probe to measure the esterase activity of individual mitochondria. This approach enabled us to investigate the influence of temperature, pH, metal ions, and various compounds on the mitochondrial esterase activity without any interference from other cellular constituents. Interestingly, we observed a decline in the mitochondrial esterase activity following the administration of mitochondrial respiratory chain inhibitors. Furthermore, we found that mitochondrial esterase activity was notably higher in the presence of a high concentration of ATP compared to that of ADP and AMP. Additionally, we noticed a correlation between elevated levels of complex IV and increased mitochondrial esterase activity. These findings suggest a functional connection between the mitochondrial respiratory chain and mitochondrial esterase activity. Moreover, we detected an upsurge in mitochondrial esterase activity during the early stages of apoptosis, while cellular esterase activity decreased. This highlights the significance of analyzing enzyme activity within specific organelle subregions. In summary, the integration of a nano-flow cytometer and fluorescent dyes introduces a novel method for quantifying mitochondrial enzyme activity with the potential to uncover the alterations and unique functions of other mitochondrial enzymes.
    MeSH term(s) Mitochondria/metabolism ; Fluorescent Dyes/chemistry ; Apoptosis ; Mitochondrial Membranes ; Esterases/metabolism
    Chemical Substances Fluorescent Dyes ; Esterases (EC 3.1.-)
    Language English
    Publishing date 2024-01-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c04321
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Molecular modeling, expression and wound-repair activity in vivo of platelet-derived growth factor from Periplaneta americana

    Lu, Kaimin / Wu, Chuanfang / Bao, Jinku

    Process biochemistry. 2021 Nov., v. 110

    2021  

    Abstract: Platelet-derived growth factors (PDGFs) promote the repair of the wounded tissue by stimulating macrophages to produce and secrete growth factors. Active constituents extracted from Periplaneta americana were primary for treatment of wounds, pressure ... ...

    Abstract Platelet-derived growth factors (PDGFs) promote the repair of the wounded tissue by stimulating macrophages to produce and secrete growth factors. Active constituents extracted from Periplaneta americana were primary for treatment of wounds, pressure sores, and burns. In this study, PDGF from Periplaneta americana (PAPDGF) was identified, and the sequence analysis, physical properties, 3D structure of PAPDGF and the interfaces of PAPDGF-PDGFRβ were predicted by bioinformatics. In addition, to explore the relationship between the key amino acid residues and activity of PAPDGF, recombinant mutant of PAPDGF (rmPAPDGF, D164H) was constructed. The sequence of PAPDGF and mutant of PAPDGF (D164H) were expressed and purified by Ni-affinity chromatography. The wound-repair activity of recombinant PAPDGF (rPAPDGF) and rmPAPDGF were tested in vivo. The results showed that rPAPDGF and rmPAPDGF were successfully obtained and exhibited a single band on SDS-PAGE at 49.87 kDa. The rPAPDGF showed potent repair activity by stimulating the expression of collagen and PDGFRβ, whereas the rmPAPDGF exhibited low repair activity in vivo, which indicated that the residue D164 in PAPDGF was the key amino acid to maintain activity. Taken together, PAPDGF may be served as a promising candidate agent for the treatment of wound in the future.
    Keywords Periplaneta americana ; amino acids ; bioinformatics ; chromatography ; collagen ; macrophages ; mutants ; platelet-derived growth factor ; polyacrylamide gel electrophoresis ; sequence analysis
    Language English
    Dates of publication 2021-11
    Size p. 151-162.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 1359-5113
    DOI 10.1016/j.procbio.2021.08.009
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: A cationic iridium(III) complex containing a thiosemicarbazide unit: Synthesis and application for turn-on chemiluminescent detection of Hg

    Tang, Huaijun / Wang, Yuhong / Chen, Zeyu / Yang, Kaixin / Qin, Jing / Li, Xianghua / Li, Haoju / Gao, Long / Lu, Shiyou / Wang, Kaimin

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

    2022  Volume 279, Page(s) 121396

    Abstract: A novel cationic iridium(III) complex [(ppy) ...

    Abstract A novel cationic iridium(III) complex [(ppy)
    MeSH term(s) Cations ; Iridium ; Mercury ; Semicarbazides
    Chemical Substances Cations ; Semicarbazides ; Iridium (44448S9773) ; thiosemicarbazide (6056O8W6ET) ; Mercury (FXS1BY2PGL)
    Language English
    Publishing date 2022-05-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 210413-1
    ISSN 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
    ISSN (online) 1873-3557
    ISSN 0370-8322 ; 0584-8539 ; 1386-1425
    DOI 10.1016/j.saa.2022.121396
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 51/98: Show issues
    Z 1611 a: Show issues
    924/340: Show issues

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  6. Article ; Online: Correction: Engineering Cu

    Du, Kaimin / Zhao, Shuang / Feng, Jing / Gao, Xuan / Liu, Kai / Wang, Xiaozhen / Zhang, Manli / Li, Yao / Lu, Yu / Zhang, Hongjie

    Journal of materials chemistry. B

    2021  Volume 9, Issue 19, Page(s) 4065–4067

    Abstract: Correction for 'Engineering ... ...

    Abstract Correction for 'Engineering Cu
    Language English
    Publishing date 2021-05-19
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/d1tb90070f
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  7. Article ; Online: Periplaneta americana

    Lu, Kaimin / Zhou, Jing / Deng, Jie / Li, Yangjun / Wu, Chuanfang / Bao, Jinku

    Molecules (Basel, Switzerland)

    2021  Volume 26, Issue 6

    Abstract: The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects. ...

    Abstract The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects.
    MeSH term(s) Acute Disease ; Animals ; Anti-Inflammatory Agents/chemistry ; Anti-Inflammatory Agents/pharmacology ; Colitis/drug therapy ; Colitis/immunology ; Colitis/microbiology ; Disease Models, Animal ; Gastrointestinal Microbiome/drug effects ; Immunomodulation/drug effects ; Male ; Mice ; Oligosaccharides/chemistry ; Oligosaccharides/pharmacology ; Periplaneta/chemistry
    Chemical Substances Anti-Inflammatory Agents ; Oligosaccharides
    Language English
    Publishing date 2021-03-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26061718
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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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  8. Article ; Online: Extravillous trophoblast cell-derived exosomes induce vascular smooth muscle cell apoptosis via a mechanism associated with miR-143-3p.

    Liu, Hanbo / Chen, Miaojuan / Ning, Fen / Ye, Yixin / Lu, Qinsheng / Lu, Shenjiao / Duan, Yaoyun / Gan, Xiaowen / Zhao, Mingguang / Guo, Kaimin / Lash, Gendie E

    Molecular human reproduction

    2023  Volume 29, Issue 8

    Abstract: The remodeling of uterine spiral arteries is a complex process requiring the dynamic action of various cell types. During early pregnancy, extravillous trophoblast (EVT) cells differentiate and invade the vascular wall, replacing the vascular smooth ... ...

    Abstract The remodeling of uterine spiral arteries is a complex process requiring the dynamic action of various cell types. During early pregnancy, extravillous trophoblast (EVT) cells differentiate and invade the vascular wall, replacing the vascular smooth muscle cells (VSMCs). Several in vitro studies have shown that EVT cells play an important role in promoting VSMC apoptosis, however, the mechanism underlying this process is not fully understood. In this study, we demonstrated that EVT-conditioned media and EVT-derived exosomes could induce VSMC apoptosis. Through data mining and experimental verification, it was demonstrated that the EVT exosome miR-143-3p induced VSMC apoptosis in both VSMCs and a chorionic plate artery (CPA) model. Furthermore, FAS ligand was also expressed on the EVT exosomes and may play a co-ordinated role in apoptosis induction. These data clearly demonstrated that VSMC apoptosis is mediated by EVT-derived exosomes and their cargo of miR-143-3p as well as their cell surface presentation of FASL. This finding increases our understanding of the molecular mechanisms underlying the regulation of VSMC apoptosis during spiral artery remodeling.
    MeSH term(s) Pregnancy ; Female ; Humans ; Trophoblasts/metabolism ; Muscle, Smooth, Vascular/metabolism ; Exosomes/genetics ; Uterine Artery/metabolism ; Apoptosis/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Myocytes, Smooth Muscle/metabolism
    Chemical Substances MicroRNAs ; MIRN143 microRNA, human
    Language English
    Publishing date 2023-06-27
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1324348-2
    ISSN 1460-2407 ; 1360-9947
    ISSN (online) 1460-2407
    ISSN 1360-9947
    DOI 10.1093/molehr/gaad026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4482: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Periplaneta americana Oligosaccharides Exert Anti-Inflammatory Activity through Immunoregulation and Modulation of Gut Microbiota in Acute Colitis Mice Model

    Kaimin Lu / Jing Zhou / Jie Deng / Yangjun Li / Chuanfang Wu / Jinku Bao

    Molecules, Vol 26, Iss 1718, p

    2021  Volume 1718

    Abstract: The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects. Periplaneta americana ...

    Abstract The incidence and prevalence of inflammatory bowel disorders (IBD) are increasing around the world due to bacterial infection, abnormal immune response, etc. The conventional medicines for IBD treatment possess serious side effects. Periplaneta americana ( P. americana ), a traditional Chinese medicine, has been used to treat arthritis, fever, aches, inflammation, and other diseases. This study aimed to evaluate the anti-inflammatory effects of oligosaccharides from P. Americana (OPA) and its possible mechanisms in vivo. OPA were purified and biochemical characterization was analyzed by HPGPC, HPLC, FT-IR, and GC–MS. Acute colitis mice model was established, the acute toxicity and anti-inflammatory activity were tested in vivo. The results showed OPA with molecular mass of 1.0 kDa were composed of 83% glucose, 6% galactose, 11% xylose, and the backbone was (1→4)-Glcp. OPA had potent antioxidant activities in vitro and significantly alleviated the clinical symptoms of colitis, relieved colon damage without toxic side effects in vivo. OPA exhibited anti-inflammatory activity by regulating Th1/Th2, reducing oxidative stress, preserving intestinal barrier integrity, and inhibiting TLR4/MAPK/NF-κB pathway. Moreover, OPA protected gut by increasing microbial diversity and beneficial bacteria, and reducing pathogenic bacteria in feces. OPA might be the candidate of complementary and alternative medicines of IBD with low-cost and high safety.
    Keywords inflammatory bowel disorder ; Periplaneta americana ; oligosaccharide ; gut microbiota ; immune ; oxidative stress ; Organic chemistry ; QD241-441
    Subject code 610
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Near-Infrared-Light-Responsive Copper Oxide Nanoparticles as Efficient Theranostic Nanoagents for Photothermal Tumor Ablation.

    Gao, Xuan / Zhang, Peng / Du, Kaimin / Zhang, Manli / Wen, Ding / Lu, Yu / Feng, Jing / Zhang, Hongjie

    ACS applied bio materials

    2021  Volume 4, Issue 6, Page(s) 5266–5275

    Abstract: A theranostic nanoagent exhibits great promise to improve diagnostic accuracy and therapy efficacy. Herein, a kind of theranostic nanoagent based on poly(vinylpyrrolidone) (PVP)-protected ultrasmall ... ...

    Abstract A theranostic nanoagent exhibits great promise to improve diagnostic accuracy and therapy efficacy. Herein, a kind of theranostic nanoagent based on poly(vinylpyrrolidone) (PVP)-protected ultrasmall Cu
    MeSH term(s) Copper ; Humans ; Nanoparticles/therapeutic use ; Neoplasms/diagnostic imaging ; Oxides ; Theranostic Nanomedicine/methods
    Chemical Substances Oxides ; Copper (789U1901C5)
    Language English
    Publishing date 2021-06-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2576-6422
    ISSN (online) 2576-6422
    DOI 10.1021/acsabm.1c00410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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