LIVIVO - Search results -

Search results

Result 1 - 10 of total 20

Search options

Article ; Online: CDK activity at the centrosome regulates the cell cycle.

Roberts, Emma L / Greenwood, Jessica / Kapadia, Nitin / Auchynnikava, Tania / Basu, Souradeep / Nurse, Paul

2024 Volume 43, Issue 4, Page(s) 114066

Abstract: In human cells and yeast, an intact "hydrophobic patch" substrate docking site is needed for mitotic cyclin centrosomal localization. A hydrophobic patch mutant (HPM) of the fission yeast mitotic cyclin Cdc13 cannot enter mitosis, but whether this is due ...

Abstract	In human cells and yeast, an intact "hydrophobic patch" substrate docking site is needed for mitotic cyclin centrosomal localization. A hydrophobic patch mutant (HPM) of the fission yeast mitotic cyclin Cdc13 cannot enter mitosis, but whether this is due to defective centrosomal localization or defective cyclin-substrate docking more widely is unknown. Here, we show that artificially restoring Cdc13-HPM centrosomal localization promotes mitotic entry and increases CDK (cyclin-dependent kinase) substrate phosphorylation at the centrosome and in the cytoplasm. We also show that the S-phase B-cyclin hydrophobic patch is required for centrosomal localization but not for S phase. We propose that the hydrophobic patch is essential for mitosis due to its requirement for the local concentration of cyclin-CDK with CDK substrates and regulators at the centrosome. Our findings emphasize the central importance of the centrosome as a hub coordinating cell-cycle control and explain why the cyclin hydrophobic patch is essential for mitosis.
MeSH term(s)	Centrosome/metabolism ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/metabolism ; Cyclin-Dependent Kinases/metabolism ; Cell Cycle ; Mitosis ; Phosphorylation ; Cell Cycle Proteins/metabolism ; Cell Cycle Proteins/genetics ; Hydrophobic and Hydrophilic Interactions ; Humans ; Cyclin B
Chemical Substances	Schizosaccharomyces pombe Proteins ; Cyclin-Dependent Kinases (EC 2.7.11.22) ; Cdc13 protein, S pombe ; Cell Cycle Proteins ; Cyclin B
Language	English
Publishing date	2024-04-04
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649101-1
ISSN	2211-1247 ; 2211-1247
ISSN (online)	2211-1247
ISSN	2211-1247
DOI	10.1016/j.celrep.2024.114066
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Core control principles of the eukaryotic cell cycle.

Basu, Souradeep / Greenwood, Jessica / Jones, Andrew W / Nurse, Paul

Nature

2022 Volume 607, Issue 7918, Page(s) 381–386

Abstract: Cyclin-dependent kinases (CDKs) lie at the heart of eukaryotic cell cycle control, with different cyclin-CDK complexes initiating DNA replication (S-CDKs) and mitosis (M-CDKs) ...

Abstract	Cyclin-dependent kinases (CDKs) lie at the heart of eukaryotic cell cycle control, with different cyclin-CDK complexes initiating DNA replication (S-CDKs) and mitosis (M-CDKs)
MeSH term(s)	Cell Cycle ; Centrosome ; Cyclin-Dependent Kinases/metabolism ; Cyclins/metabolism ; Eukaryotic Cells/cytology ; Eukaryotic Cells/enzymology ; Eukaryotic Cells/metabolism ; Mitosis ; Models, Biological ; Phosphoproteins/metabolism ; Phosphorylation ; Protein Phosphatase 1 ; Proteomics ; S Phase ; Schizosaccharomyces/cytology ; Schizosaccharomyces/enzymology ; Schizosaccharomyces/metabolism ; Substrate Specificity
Chemical Substances	Cyclins ; Phosphoproteins ; Cyclin-Dependent Kinases (EC 2.7.11.22) ; Protein Phosphatase 1 (EC 3.1.3.16)
Language	English
Publishing date	2022-06-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	120714-3
ISSN	1476-4687 ; 0028-0836
ISSN (online)	1476-4687
ISSN	0028-0836
DOI	10.1038/s41586-022-04798-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 26: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 9: Show issues
Zs.MO 244: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A CDK activity buffer ensures mitotic completion.

Basu, Souradeep / Patterson, James O / Zeisner, Theresa U / Nurse, Paul

Journal of cell science

2022 Volume 135, Issue 12

Abstract: The eukaryotic cell cycle is driven by the activity of cyclin-dependent kinases (CDKs). CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. However, it is not known whether the entire range of CDK ... ...

Abstract	The eukaryotic cell cycle is driven by the activity of cyclin-dependent kinases (CDKs). CDK activity rises over 50-fold during the cell cycle, from a low level in G1 to a high level in mitosis. However, it is not known whether the entire range of CDK activity is necessary for cell cycle progression, or whether cells can tolerate a reduction in CDK activity level. Here, in fission yeast, we show that sublethal CDK inhibition lengthens the time cells spend in mitosis but does not cause misordering of mitotic events. Maximum attainable CDK activity exceeds the amount necessary for mitosis, and thus forms a CDK activity buffer between sufficient and maximal possible CDK activities. This CDK activity buffer is needed for mitotic completion when CDK activity is compromised, and CDK inhibition only becomes lethal to cells when this buffer is exhausted. Finally, we explore what factors influence this CDK activity buffer, and find that it is influenced by CDK-counteracting phosphatases. Therefore, maximum attainable CDK activity is not necessary for mitosis but provides robustness to CDK activity reduction to ensure mitotic completion.
MeSH term(s)	Cell Cycle Proteins/metabolism ; Cyclin-Dependent Kinases/genetics ; Cyclin-Dependent Kinases/metabolism ; Mitosis ; Phosphorylation ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/genetics ; Schizosaccharomyces pombe Proteins/metabolism
Chemical Substances	Cell Cycle Proteins ; Schizosaccharomyces pombe Proteins ; Cyclin-Dependent Kinases (EC 2.7.11.22)
Language	English
Publishing date	2022-06-21
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2993-2
ISSN	1477-9137 ; 0021-9533
ISSN (online)	1477-9137
ISSN	0021-9533
DOI	10.1242/jcs.259626
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1200: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 14: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Imide arylation with aryl(TMP)iodonium tosylates.

Basu, Souradeep / Sandtorv, Alexander H / Stuart, David R

Beilstein journal of organic chemistry

2018 Volume 14, Page(s) 1034–1038

Abstract: Herein, we describe the synthesis ... ...

Abstract	Herein, we describe the synthesis of
Language	English
Publishing date	2018-05-11
Publishing country	Germany
Document type	Journal Article
ZDB-ID	2192461-2
ISSN	1860-5397
ISSN	1860-5397
DOI	10.3762/bjoc.14.90
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: CDK control pathways integrate cell size and ploidy information to control cell division

James Oliver Patterson / Souradeep Basu / Paul Rees / Paul Nurse

eLife, Vol

2021 Volume 10

Abstract: Maintenance of cell size homeostasis is a property that is conserved throughout eukaryotes. Cell size homeostasis is brought about by the co-ordination of cell division with cell growth and requires restriction of smaller cells from undergoing mitosis ... ...

Abstract	Maintenance of cell size homeostasis is a property that is conserved throughout eukaryotes. Cell size homeostasis is brought about by the co-ordination of cell division with cell growth and requires restriction of smaller cells from undergoing mitosis and cell division, whilst allowing larger cells to do so. Cyclin-CDK is the fundamental driver of mitosis and therefore ultimately ensures size homeostasis. Here we dissect determinants of CDK activity in vivo to investigate how cell size information is processed by the cell cycle network in fission yeast. We develop a high-throughput single-cell assay system of CDK activity in vivo and show that inhibitory tyrosine phosphorylation of CDK encodes cell size information, with the phosphatase PP2A aiding to set a size threshold for division. CDK inhibitory phosphorylation works synergistically with PP2A to prevent mitosis in smaller cells. Finally, we find that diploid cells of equivalent size to haploid cells exhibit lower CDK activity in response to equal cyclin-CDK enzyme concentrations, suggesting that CDK activity is reduced by increased DNA levels. Therefore, scaling of cyclin-CDK levels with cell size, CDK inhibitory phosphorylation, PP2A, and DNA-dependent inhibition of CDK activity, all inform the cell cycle network of cell size, thus contributing to cell size homeostasis.
Keywords	cell cycle ; synthetic biology ; cell size control ; CDK ; cyclin ; PP2A ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	eLife Sciences Publications Ltd
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: CDK control pathways integrate cell size and ploidy information to control cell division.

Patterson, James Oliver / Basu, Souradeep / Rees, Paul / Nurse, Paul

eLife

2021 Volume 10

Abstract	Maintenance of cell size homeostasis is a property that is conserved throughout eukaryotes. Cell size homeostasis is brought about by the co-ordination of cell division with cell growth and requires restriction of smaller cells from undergoing mitosis and cell division, whilst allowing larger cells to do so. Cyclin-CDK is the fundamental driver of mitosis and therefore ultimately ensures size homeostasis. Here we dissect determinants of CDK activity in vivo to investigate how cell size information is processed by the cell cycle network in fission yeast. We develop a high-throughput single-cell assay system of CDK activity in vivo and show that inhibitory tyrosine phosphorylation of CDK encodes cell size information, with the phosphatase PP2A aiding to set a size threshold for division. CDK inhibitory phosphorylation works synergistically with PP2A to prevent mitosis in smaller cells. Finally, we find that diploid cells of equivalent size to haploid cells exhibit lower CDK activity in response to equal cyclin-CDK enzyme concentrations, suggesting that CDK activity is reduced by increased DNA levels. Therefore, scaling of cyclin-CDK levels with cell size, CDK inhibitory phosphorylation, PP2A, and DNA-dependent inhibition of CDK activity, all inform the cell cycle network of cell size, thus contributing to cell size homeostasis.
MeSH term(s)	Cell Cycle ; Cell Division ; Cell Size ; Cyclin-Dependent Kinases/genetics ; Cyclin-Dependent Kinases/metabolism ; Cyclins/genetics ; Cyclins/metabolism ; Homeostasis ; Mitosis ; Phosphorylation ; Ploidies ; Protein Phosphatase 2/metabolism ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/genetics ; Schizosaccharomyces pombe Proteins/metabolism
Chemical Substances	Cyclins ; Schizosaccharomyces pombe Proteins ; Cyclin-Dependent Kinases (EC 2.7.11.22) ; Protein Phosphatase 2 (EC 3.1.3.16)
Language	English
Publishing date	2021-06-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2687154-3
ISSN	2050-084X ; 2050-084X
ISSN (online)	2050-084X
ISSN	2050-084X
DOI	10.7554/eLife.64592
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Imide arylation with aryl(TMP)iodonium tosylates

Souradeep Basu / Alexander H. Sandtorv / David R. Stuart

Beilstein Journal of Organic Chemistry, Vol 14, Iss 1, Pp 1034-

2018 Volume 1038

Abstract: Herein, we describe the synthesis of N-aryl phthalimides by metal-free coupling of potassium phthalimide with unsymmetrical aryl(TMP)iodonium tosylate salts. The aryl transfer from the iodonium moiety occurs under electronic control with the electron- ... ...

Abstract	Herein, we describe the synthesis of N-aryl phthalimides by metal-free coupling of potassium phthalimide with unsymmetrical aryl(TMP)iodonium tosylate salts. The aryl transfer from the iodonium moiety occurs under electronic control with the electron-rich trimethoxyphenyl group acting as a competent dummy ligand. The yields of N-aryl phthalimides are moderate to high and the coupling reaction is compatible with electron-deficient and sterically encumbered aryl groups.
Keywords	arylation ; C–N coupling ; diaryliodonium ; hypercoordinate iodine ; metal-free ; Science ; Q ; Organic chemistry ; QD241-441
Language	English
Publishing date	2018-05-01T00:00:00Z
Publisher	Beilstein-Institut
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Upper respiratory tract microbiome profiles in SARS-CoV-2 Delta and Omicron infected patients exhibit variant specific patterns and robust prediction of disease groups.

Nath, Shankha / Sarkar, Mousumi / Maddheshiya, Ankita / De, Debjit / Paul, Shouvik / Dey, Souradeep / Pal, Kuhu / Roy, Suman Kr / Ghosh, Ayan / Sengupta, Sharmila / Paine, Suman Kalyan / Biswas, Nidhan K / Basu, Analabha / Mukherjee, Souvik

Microbiology spectrum

2023 Volume 11, Issue 6, Page(s) e0236823

Abstract: Importance: The role of the upper respiratory tract (URT) microbiome in predicting lung health has been documented in several studies. The dysbiosis in COVID patients has been associated with disease outcomes by modulating the host immune system. ... ...

Abstract	Importance: The role of the upper respiratory tract (URT) microbiome in predicting lung health has been documented in several studies. The dysbiosis in COVID patients has been associated with disease outcomes by modulating the host immune system. However, although it has been known that different SARS-CoV-2 variants manifest distinct transmissibility and mortality rates in human populations, their effect on the composition and diversity of the URT microbiome has not been studied to date. Unlike the older variant (Delta), the newer variant (Omicron) have become more transmissible with lesser mortality and the symptoms have also changed significantly. Hence, in the present study, we have investigated the change in the URT microbiome associated with Delta and Omicron variants and identified variant-specific signatures that will be useful in the assessment of lung health and can be utilized for nasal probiotic therapy in the future.
MeSH term(s)	Humans ; SARS-CoV-2/genetics ; COVID-19 ; Microbiota/genetics ; Nose
Language	English
Publishing date	2023-10-31
Publishing country	United States
Document type	Journal Article
ZDB-ID	2807133-5
ISSN	2165-0497 ; 2165-0497
ISSN (online)	2165-0497
ISSN	2165-0497
DOI	10.1128/spectrum.02368-23
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: The Hydrophobic Patch Directs Cyclin B to Centrosomes to Promote Global CDK Phosphorylation at Mitosis.

Basu, Souradeep / Roberts, Emma L / Jones, Andrew W / Swaffer, Matthew P / Snijders, Ambrosius P / Nurse, Paul

Current biology : CB

2020 Volume 30, Issue 5, Page(s) 883–892.e4

Abstract: The cyclin-dependent kinases (CDKs) are the major cell-cycle regulators that phosphorylate hundreds of substrates, controlling the onset of S phase and M phase [1-3]. However, the patterns of substrate phosphorylation increase are not uniform, as ... ...

Abstract	The cyclin-dependent kinases (CDKs) are the major cell-cycle regulators that phosphorylate hundreds of substrates, controlling the onset of S phase and M phase [1-3]. However, the patterns of substrate phosphorylation increase are not uniform, as different substrates become phosphorylated at different times as cells proceed through the cell cycle [4, 5]. In fission yeast, the correct ordering of CDK substrate phosphorylation can be established by the activity of a single mitotic cyclin-CDK complex [6, 7]. Here, we investigate the substrate-docking region, the hydrophobic patch, on the fission yeast mitotic cyclin Cdc13 as a potential mechanism to correctly order CDK substrate phosphorylation. We show that the hydrophobic patch targets Cdc13 to the yeast centrosome equivalent, the spindle pole body (SPB), and disruption of this motif prevents both centrosomal localization of Cdc13 and the onset of mitosis but does not prevent S phase. CDK phosphorylation in mitosis is compromised for approximately half of all mitotic CDK substrates, with substrates affected generally being those that require the highest levels of CDK activity to become phosphorylated and those that are located at the SPB. Our experiments suggest that the hydrophobic patch of mitotic cyclins contributes to CDK substrate selection by directing the localization of Cdc13-CDK to centrosomes and that this localization of CDK contributes to the CDK substrate phosphorylation necessary to ensure proper entry into mitosis. Finally, we show that mutation of the hydrophobic patch prevents cyclin B1 localization to centrosomes in human cells, suggesting that this mechanism of cyclin-CDK spatial regulation may be conserved across eukaryotes.
MeSH term(s)	Cell Line ; Centrosome/metabolism ; Cyclin B1/metabolism ; Cyclin-Dependent Kinases/metabolism ; Humans ; Hydrophobic and Hydrophilic Interactions ; Phosphorylation ; Schizosaccharomyces/metabolism ; Schizosaccharomyces pombe Proteins/metabolism
Chemical Substances	CCNB1 protein, human ; Cyclin B1 ; Schizosaccharomyces pombe Proteins ; Cyclin-Dependent Kinases (EC 2.7.11.22)
Language	English
Publishing date	2020-02-20
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1071731-6
ISSN	1879-0445 ; 0960-9822
ISSN (online)	1879-0445
ISSN	0960-9822
DOI	10.1016/j.cub.2019.12.053
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 3208: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Synthesis of Crescent Shaped Heterocycle-fused Aromatics via Garratt-Braverman Cyclization and Their DNA-binding Studies

Ghosh, Debaki / Souradeep Basu / Monisha Singha / Joyee Das / Prabuddha Bhattacharya / Amit Basak

Tetrahedron letters. 2017,

2017

Abstract: Three crescent shaped heterocycle-fused phenanthrene based systems 1-3 have been synthesized starting from benzene (or substituted benzene) 1,2-bis-propargyl alcohols. Bis-alkylation with propargylic bromides provided the key intermediate, the bis- ... ...

Abstract	Three crescent shaped heterocycle-fused phenanthrene based systems 1-3 have been synthesized starting from benzene (or substituted benzene) 1,2-bis-propargyl alcohols. Bis-alkylation with propargylic bromides provided the key intermediate, the bis-propargyl bis-ethers. In spite of the possibility of many competing reactions, the latter underwent facile double Garratt-Braverman Cyclization to provide compounds 1-3 in near quantitative yield, in a striking reaction involving the formation of four C-C bonds in a single step. Compounds 1-3 showed binding interaction with DNA, predominantly, via groove binding along with partial intercalation (combilexins). Molecular docking study supported the propsed binding modes.
Keywords	DNA ; alcohols ; benzene ; bromides ; chemical bonding ; chemical reactions ; chemical structure ; molecular models ; phenanthrene
Language	English
Size	p. .
Publishing place	Elsevier Ltd
Document type	Article
Note	Pre-press version
ZDB-ID	204287-3
ISSN	1873-3581 ; 0040-4039
ISSN (online)	1873-3581
ISSN	0040-4039
DOI	10.1016/j.tetlet.2017.04.003
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 2837: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito