LIVIVO - Search results -

Search results

Result 1 - 10 of total 27

Search options

Article ; Online: Recently activated CD4 T cells in tuberculosis express OX40 as a target for host-directed immunotherapy.

Gress, Abigail R / Ronayne, Christine E / Thiede, Joshua M / Meyerholz, David K / Okurut, Samuel / Stumpf, Julia / Mathes, Tailor V / Ssebambulidde, Kenneth / Meya, David B / Cresswell, Fiona V / Boulware, David R / Bold, Tyler D

Nature communications

2023 Volume 14, Issue 1, Page(s) 8423

Abstract: After Mycobacterium tuberculosis (Mtb) infection, many effector T cells traffic to the lungs, but few become activated. Here we use an antigen receptor reporter mouse (Nur77-GFP) to identify recently activated CD4 T cells in the lungs. These Nur77- ... ...

Abstract	After Mycobacterium tuberculosis (Mtb) infection, many effector T cells traffic to the lungs, but few become activated. Here we use an antigen receptor reporter mouse (Nur77-GFP) to identify recently activated CD4 T cells in the lungs. These Nur77-GFP
MeSH term(s)	Humans ; Mice ; Animals ; CD4-Positive T-Lymphocytes ; Receptors, OX40/agonists ; CD8-Positive T-Lymphocytes ; Immunotherapy ; Tuberculosis/therapy
Chemical Substances	Receptors, OX40
Language	English
Publishing date	2023-12-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-023-44152-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Cephem-Pyrazinoic Acid Conjugates: Circumventing Resistance in Mycobacterium tuberculosis.

Cole, Malcolm S / Howe, Michael D / Buonomo, Joseph A / Sharma, Sachin / Lamont, Elise A / Brody, Scott I / Mishra, Neeraj K / Minato, Yusuke / Thiede, Joshua M / Baughn, Anthony D / Aldrich, Courtney C

Chemistry (Weinheim an der Bergstrasse, Germany)

2022 Volume 28, Issue 51, Page(s) e202200995

Abstract: ... therapeutics. β-lactam antibiotics have traditionally been ineffective against M. tuberculosis (Mtb ... drug. These conjugates are selectively active against M. tuberculosis and related mycobacteria, and ...

Abstract	Tuberculosis (TB) is a leading source of infectious disease mortality globally. Antibiotic-resistant strains comprise an estimated 10 % of new TB cases and present an urgent need for novel therapeutics. β-lactam antibiotics have traditionally been ineffective against M. tuberculosis (Mtb), the causative agent of TB, due to the organism's inherent expression of β-lactamases that destroy the electrophilic β-lactam warhead. We have developed novel β-lactam conjugates, which exploit this inherent β-lactamase activity to achieve selective release of pyrazinoic acid (POA), the active form of a first-line TB drug. These conjugates are selectively active against M. tuberculosis and related mycobacteria, and activity is retained or even potentiated in multiple resistant strains and models. Preliminary mechanistic investigations suggest that both the POA "warhead" as well as the β-lactam "promoiety" contribute to the observed activity, demonstrating a codrug strategy with important implications for future TB therapy.
MeSH term(s)	Antitubercular Agents/pharmacology ; Antitubercular Agents/therapeutic use ; Humans ; Microbial Sensitivity Tests ; Mycobacterium tuberculosis ; Pyrazinamide/analogs & derivatives ; Pyrazinamide/pharmacology ; Tuberculosis/drug therapy ; Tuberculosis/microbiology ; beta-Lactams/pharmacology
Chemical Substances	Antitubercular Agents ; beta-Lactams ; Pyrazinamide (2KNI5N06TI) ; pyrazinoic acid (2WB23298SP)
Language	English
Publishing date	2022-07-27
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1478547-X
ISSN	1521-3765 ; 0947-6539
ISSN (online)	1521-3765
ISSN	0947-6539
DOI	10.1002/chem.202200995
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

Article ; Online: Pyrazinamide Susceptibility Is Driven by Activation of the SigE-Dependent Cell Envelope Stress Response in Mycobacterium tuberculosis.

Thiede, Joshua M / Dillon, Nicholas A / Howe, Michael D / Aflakpui, Ranee / Modlin, Samuel J / Hoffner, Sven E / Valafar, Faramarz / Minato, Yusuke / Baughn, Anthony D

mBio

2022 Volume 13, Issue 1, Page(s) e0043921

Abstract: Pyrazinamide (PZA) plays a crucial role in first-line tuberculosis drug therapy. Unlike other antimicrobial agents, PZA is active against Mycobacterium tuberculosis only at low pH. The basis for this conditional drug susceptibility remains undefined. In ... ...

Abstract	Pyrazinamide (PZA) plays a crucial role in first-line tuberculosis drug therapy. Unlike other antimicrobial agents, PZA is active against Mycobacterium tuberculosis only at low pH. The basis for this conditional drug susceptibility remains undefined. In this study, we utilized a genome-wide approach to interrogate potentiation of PZA action. We found that mutations in numerous genes involved in central metabolism as well as cell envelope maintenance and stress response are associated with PZA resistance. Further, we demonstrate that constitutive activation of the cell envelope stress response can drive PZA susceptibility independent of environmental pH. Consequently, exposure to peptidoglycan synthesis inhibitors, such as beta-lactams and d-cycloserine, potentiate PZA action through triggering this response. These findings illuminate a regulatory mechanism for conditional PZA susceptibility and reveal new avenues for enhancing potency of this important drug through targeting activation of the cell envelope stress response.
MeSH term(s)	Humans ; Pyrazinamide/therapeutic use ; Mycobacterium tuberculosis/genetics ; Amidohydrolases/metabolism ; Antitubercular Agents/pharmacology ; Tuberculosis/microbiology ; Mutation ; Microbial Sensitivity Tests
Chemical Substances	Pyrazinamide (2KNI5N06TI) ; Amidohydrolases (EC 3.5.-) ; Antitubercular Agents
Language	English
Publishing date	2022-02-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.00439-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.

Minato, Yusuke / Gohl, Daryl M / Thiede, Joshua M / Chacón, Jeremy M / Harcombe, William R / Maruyama, Fumito / Baughn, Anthony D

mSystems

2019 Volume 4, Issue 4

Abstract: A better understanding of essential cellular functions in pathogenic bacteria is important for the development of more effective antimicrobial agents. We performed a comprehensive identification of essential genes ... ...

Abstract	A better understanding of essential cellular functions in pathogenic bacteria is important for the development of more effective antimicrobial agents. We performed a comprehensive identification of essential genes in
Language	English
Publishing date	2019-06-25
Publishing country	United States
Document type	Journal Article
ISSN	2379-5077
ISSN	2379-5077
DOI	10.1128/mSystems.00070-19
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Immune Profiling to Determine Early Disease Trajectories Associated With Coronavirus Disease 2019 Mortality Rate: A Substudy from the ACTT-1 Trial.

Thiede, Joshua M / Gress, Abigail R / Libby, Samuel D / Ronayne, Christine E / Matchett, William E / Noren, Brooke / Billings, Joanne L / Menachery, Vineet D / Langlois, Ryan A / Kline, Susan / Bold, Tyler D

The Journal of infectious diseases

2021 Volume 223, Issue 8, Page(s) 1339–1344

Abstract: Coronavirus disease 2019 (COVID-19) outcomes are linked to host immune responses and may be affected by antiviral therapy. We investigated antibody and cytokine responses in ACTT-1 study participants enrolled at our center. We studied serum specimens ... ...

Abstract	Coronavirus disease 2019 (COVID-19) outcomes are linked to host immune responses and may be affected by antiviral therapy. We investigated antibody and cytokine responses in ACTT-1 study participants enrolled at our center. We studied serum specimens from 19 hospitalized adults with COVID-19 randomized to treatment with remdesivir or placebo. We assessed severe acute respiratory syndrome coronavirus 2 antibody responses and identified cytokine signatures, using hierarchical clustering. We identified no clear immunologic trends attributable to remdesivir treatment. Seven participants were initially seronegative at study enrollment, and all 4 deaths occurred in this group with more recent symptom onset. We identified 3 dominant cytokine signatures, demonstrating different disease trajectories.
MeSH term(s)	Adenosine Monophosphate/analogs & derivatives ; Adenosine Monophosphate/immunology ; Adenosine Monophosphate/therapeutic use ; Adult ; Alanine/analogs & derivatives ; Alanine/immunology ; Alanine/therapeutic use ; Antibodies, Viral/immunology ; Antiviral Agents/immunology ; Antiviral Agents/therapeutic use ; COVID-19/drug therapy ; COVID-19/immunology ; COVID-19/mortality ; COVID-19/virology ; Cytokines/immunology ; Female ; Humans ; Immunity/drug effects ; Immunity/immunology ; Male ; SARS-CoV-2/drug effects ; SARS-CoV-2/immunology
Chemical Substances	Antibodies, Viral ; Antiviral Agents ; Cytokines ; remdesivir (3QKI37EEHE) ; Adenosine Monophosphate (415SHH325A) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2021-01-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	3019-3
ISSN	1537-6613 ; 0022-1899
ISSN (online)	1537-6613
ISSN	0022-1899
DOI	10.1093/infdis/jiab035
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uh II Zs.50: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG)
Zs.MO 445: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with non-severe disease.

Gniadek, Thomas J / Thiede, Joshua M / Matchett, William E / Gress, Abigail R / Pape, Kathryn A / Jenkins, Marc K / Menachery, Vineet D / Langlois, Ryan A / Bold, Tyler D

bioRxiv : the preprint server for biology

2020

Abstract: We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We ... ...

Abstract	We determined the antigen binding activity of convalescent plasma units from 47 individuals with a history of non-severe COVID-19 using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. We compared these results with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. This revealed positive correlations of varying strength (Spearman r = 0.37-0.52) between binding and neutralization. Donors age 48-75 had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75-82%) for those with the highest levels of neutralization.
Keywords	covid19
Language	English
Publishing date	2020-08-10
Publishing country	United States
Document type	Preprint
DOI	10.1101/2020.08.07.242271
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium.

Fiege, Jessica K / Thiede, Joshua M / Nanda, Hezkiel Arya / Matchett, William E / Moore, Patrick J / Montanari, Noe Rico / Thielen, Beth K / Daniel, Jerry / Stanley, Emma / Hunter, Ryan C / Menachery, Vineet D / Shen, Steven S / Bold, Tyler D / Langlois, Ryan A

PLoS pathogens

2021 Volume 17, Issue 1, Page(s) e1009292

Abstract: The human airway epithelium is the initial site of SARS-CoV-2 infection. We used flow cytometry and single cell RNA-sequencing to understand how the heterogeneity of this diverse cell population contributes to elements of viral tropism and pathogenesis, ... ...

Abstract	The human airway epithelium is the initial site of SARS-CoV-2 infection. We used flow cytometry and single cell RNA-sequencing to understand how the heterogeneity of this diverse cell population contributes to elements of viral tropism and pathogenesis, antiviral immunity, and treatment response to remdesivir. We found that, while a variety of epithelial cell types are susceptible to infection, ciliated cells are the predominant cell target of SARS-CoV-2. The host protease TMPRSS2 was required for infection of these cells. Importantly, remdesivir treatment effectively inhibited viral replication across cell types, and blunted hyperinflammatory responses. Induction of interferon responses within infected cells was rare and there was significant heterogeneity in the antiviral gene signatures, varying with the burden of infection in each cell. We also found that heavily infected secretory cells expressed abundant IL-6, a potential mediator of COVID-19 pathogenesis.
MeSH term(s)	Adenosine Monophosphate/analogs & derivatives ; Adenosine Monophosphate/pharmacology ; Alanine/analogs & derivatives ; Alanine/pharmacology ; Antiviral Agents/pharmacology ; COVID-19/genetics ; COVID-19/immunology ; COVID-19/virology ; Epithelium/immunology ; Epithelium/virology ; Humans ; Interferons/genetics ; Interferons/immunology ; Interleukin-6/genetics ; Interleukin-6/immunology ; Lung/immunology ; Lung/virology ; SARS-CoV-2/drug effects ; SARS-CoV-2/physiology ; Viral Tropism/drug effects ; Virus Replication/drug effects ; COVID-19 Drug Treatment
Chemical Substances	Antiviral Agents ; Interleukin-6 ; remdesivir (3QKI37EEHE) ; Adenosine Monophosphate (415SHH325A) ; Interferons (9008-11-1) ; Alanine (OF5P57N2ZX)
Language	English
Publishing date	2021-01-28
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2205412-1
ISSN	1553-7374 ; 1553-7374
ISSN (online)	1553-7374
ISSN	1553-7374
DOI	10.1371/journal.ppat.1009292
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: SARS-CoV-2 neutralization and serology testing of COVID-19 convalescent plasma from donors with nonsevere disease.

Gniadek, Thomas J / Thiede, Joshua M / Matchett, William E / Gress, Abigail R / Pape, Kathryn A / Fiege, Jessica K / Jenkins, Marc K / Menachery, Vineet D / Langlois, Ryan A / Bold, Tyler D

Transfusion

2020 Volume 61, Issue 1, Page(s) 17–23

Abstract: Background: The transfer of passive immunity with convalescent plasma is a promising strategy for treatment and prevention of COVID-19, but donors with a history of nonsevere disease are serologically heterogenous. The relationship between SARS-Cov-2 ... ...

Abstract	Background: The transfer of passive immunity with convalescent plasma is a promising strategy for treatment and prevention of COVID-19, but donors with a history of nonsevere disease are serologically heterogenous. The relationship between SARS-Cov-2 antigen-binding activity and neutralization activity in this population of donors has not been defined. Study design and methods: Convalescent plasma units from 47 individuals with a history of nonsevere COVID-19 were assessed for antigen-binding activity of using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS-CoV-2 targets. These results were compared with functional neutralization activity using a fluorescent reporter strain of SARS-CoV-2 in a microwell assay. Results: Positive correlations of varying strength (Spearman r = 0.37-0.52) between antigen binding and viral neutralization were identified. Donors age 48 to 75 years had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75%-82%) for those with the highest levels of neutralization. Conclusion: The strength of the relationship between antigen-binding activity and neutralization varies depending on the clinical assay used. Units in the highest tertile of binding activity for each assay are predominantly comprised of those with the greatest neutralization activity.
MeSH term(s)	Antibodies, Neutralizing/immunology ; Antibodies, Viral/immunology ; COVID-19/immunology ; COVID-19/therapy ; COVID-19 Serological Testing ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunization, Passive ; Immunoglobulin G/immunology ; SARS-CoV-2/immunology ; SARS-CoV-2/pathogenicity ; Serologic Tests
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; Immunoglobulin G
Keywords	covid19
Language	English
Publishing date	2020-10-02
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	208417-x
ISSN	1537-2995 ; 0041-1132
ISSN (online)	1537-2995
ISSN	0041-1132
DOI	10.1111/trf.16101
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 284: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Nucleocapsid vaccine elicits spike-independent SARS-CoV-2 protective immunity.

bioRxiv : the preprint server for biology

2021

Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. Neutralizing antibodies target the receptor binding domain of the spike (S) protein, a focus of successful vaccine efforts. Concerns have arisen that S- ...

Abstract	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the COVID-19 pandemic. Neutralizing antibodies target the receptor binding domain of the spike (S) protein, a focus of successful vaccine efforts. Concerns have arisen that S-specific vaccine immunity may fail to neutralize emerging variants. We show that vaccination with HAd5 expressing the nucleocapsid (N) protein can establish protective immunity, defined by reduced weight loss and viral load, in both Syrian hamsters and k18-hACE2 mice. Challenge of vaccinated mice was associated with rapid N-specific T cell recall responses in the respiratory mucosa. This study supports the rationale for including additional viral antigens, even if they are not a target of neutralizing antibodies, to broaden epitope coverage and immune effector mechanisms.
Language	English
Publishing date	2021-04-27
Publishing country	United States
Document type	Preprint
DOI	10.1101/2021.04.26.441518
Database	MEDical Literature Analysis and Retrieval System OnLINE

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Single cell resolution of SARS-CoV-2 tropism, antiviral responses, and susceptibility to therapies in primary human airway epithelium.

Jessica K Fiege / Joshua M Thiede / Hezkiel Arya Nanda / William E Matchett / Patrick J Moore / Noe Rico Montanari / Beth K Thielen / Jerry Daniel / Emma Stanley / Ryan C Hunter / Vineet D Menachery / Steven S Shen / Tyler D Bold / Ryan A Langlois

PLoS Pathogens, Vol 17, Iss 1, p e

2021 Volume 1009292

Abstract	The human airway epithelium is the initial site of SARS-CoV-2 infection. We used flow cytometry and single cell RNA-sequencing to understand how the heterogeneity of this diverse cell population contributes to elements of viral tropism and pathogenesis, antiviral immunity, and treatment response to remdesivir. We found that, while a variety of epithelial cell types are susceptible to infection, ciliated cells are the predominant cell target of SARS-CoV-2. The host protease TMPRSS2 was required for infection of these cells. Importantly, remdesivir treatment effectively inhibited viral replication across cell types, and blunted hyperinflammatory responses. Induction of interferon responses within infected cells was rare and there was significant heterogeneity in the antiviral gene signatures, varying with the burden of infection in each cell. We also found that heavily infected secretory cells expressed abundant IL-6, a potential mediator of COVID-19 pathogenesis.
Keywords	Immunologic diseases. Allergy ; RC581-607 ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED