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  1. Book ; Thesis: Die Theologie des Leibes von Papst Johannes Paul II

    Rimmel, Thomas Maria

    philosophische und theologische Grundlagen

    (Moraltheologische Studien ; N.F., 8)

    2014  

    Author's details Thomas Maria Rimmel
    Series title Moraltheologische Studien ; N.F., 8
    Keywords Leib ; Theologische Anthropologie
    Language German
    Size 531 S., 24 cm, 950 g
    Publisher EOS
    Publishing place St. Ottilien
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Augsburg, 2013
    ISBN 9783830676539 ; 3830676530
    Database Former special subject collection: coastal and deep sea fishing

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  2. Article: An optimized protocol for adenosine triphosphate quantification in T lymphocytes of lymphopenic patients

    Girardot, Thibaut / Christelle Rouget / Estellie Idealisoa / Fabienne Venet / Fanny Poujol / Guillaume Monneret / Julie Mouillaux / Julien Textoris / Thomas Rimmelé

    Journal of Immunological Methods. 2016,

    2016  

    Abstract: In several clinical contexts, the measurement of ATP concentration in T lymphocytes has been proposed as a biomarker of immune status, predictive of secondary infections. However, the use of such biomarker in lymphopenic patients requires some ... ...

    Abstract In several clinical contexts, the measurement of ATP concentration in T lymphocytes has been proposed as a biomarker of immune status, predictive of secondary infections. However, the use of such biomarker in lymphopenic patients requires some adaptations in the ATP dosage protocol. We used blood from healthy volunteers to determine the optimal experimental settings. We investigated technical aspects such as the type of anticoagulant for blood sampling, the effect of freeze and thaw cycles, the reagent and sample mixing sequence, and the optimal dilution buffer. We also shortened the incubation time to 8h, and even showed that a 30min incubation may be sufficient. To evaluate the ATP rise upon lymphocyte activation, the optimal dose of stimulant was defined to be 4μg/mL of phytohaemagglutinin. Lastly, we determined that the number of T cells needed for this measurement was as low as 50,000, which is compatible with the existing lymphopenia in clinical settings. This optimized protocol appears ready to be assessed in lymphopenic patients to further investigate the interconnection between T lymphocyte metabolism and impaired phenotype and functions.
    Keywords adenosine triphosphate ; anticoagulants ; biomarkers ; blood sampling ; lymphocyte proliferation ; metabolism ; mixing ; patients ; phenotype ; phytohemagglutinin ; secondary infection ; T-lymphocytes
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2016.10.001
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 795: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Flow cytometric evaluation of lymphocyte transformation test based on 5-ethynyl-2′deoxyuridine incorporation as a clinical alternative to tritiated thymidine uptake measurement

    Poujol, Fanny / Alain Lepape / Alexandre Pachot / Arnaud Friggeri / Christophe Malcus / Fabienne Venet / Françoise Poitevin-Later / Guillaume Monneret / Julien Textoris / Thomas Rimmelé

    Journal of Immunological Methods. 2014 Dec. 15, v. 415

    2014  

    Abstract: In clinical laboratories, the evaluation of lymphocyte proliferative response (lymphocyte transformation test—LTT) is routinely performed by the measurement of [3H]-thymidine uptake after stimulation. In this study we evaluated the performances of a ... ...

    Abstract In clinical laboratories, the evaluation of lymphocyte proliferative response (lymphocyte transformation test—LTT) is routinely performed by the measurement of [3H]-thymidine uptake after stimulation. In this study we evaluated the performances of a recently developed non-radioactive test based on the detection by flow cytometry of 5-ethynyl-2′deoxyuridine (EdU) incorporation for the measurement of LTT in routine lab conditions. After definition of optimal protocol parameters, EdU incorporation test showed good repeatability and reproducibility. Moreover, this assay was flexible enough to fit important clinical laboratory constraints (delayed stimulation, low number of cells and delayed analysis after staining). Importantly, correlations between results obtained with EdU and [3H]-thymidine incorporation assays were excellent both in healthy volunteers and pediatric and septic patients. In particular, the two techniques identified patients presenting with altered LTT. Upon confirmation in a larger cohort of patients, EdU incorporation assay may be a relevant non-radioactive candidate for LLT in clinic.
    Keywords flow cytometry ; lymphocyte proliferation ; patients ; staining ; thymidine ; tritium
    Language English
    Dates of publication 2014-1215
    Size p. 71-79.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2014.10.006
    Database NAL-Catalogue (AGRICOLA)

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 795: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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