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Article ; Online: Moonlighting Arabidopsis molybdate transporter 2 family and GSH-complex formation facilitate molybdenum homeostasis.

Weber, Jan-Niklas / Minner-Meinen, Rieke / Behnecke, Maria / Biedendieck, Rebekka / Hänsch, Veit G / Hercher, Thomas W / Hertweck, Christian / van den Hout, Lena / Knüppel, Lars / Sivov, Simon / Schulze, Jutta / Mendel, Ralf-R / Hänsch, Robert / Kaufholdt, David

Communications biology

2023 Volume 6, Issue 1, Page(s) 801

Abstract: Molybdenum (Mo) as essential micronutrient for plants, acts as active component of molybdenum cofactor (Moco). Core metabolic processes like nitrate assimilation or abscisic-acid biosynthesis rely on Moco-dependent enzymes. Although a family of molybdate ...

Abstract	Molybdenum (Mo) as essential micronutrient for plants, acts as active component of molybdenum cofactor (Moco). Core metabolic processes like nitrate assimilation or abscisic-acid biosynthesis rely on Moco-dependent enzymes. Although a family of molybdate transport proteins (MOT1) is known to date in Arabidopsis, molybdate homeostasis remained unclear. Here we report a second family of molybdate transporters (MOT2) playing key roles in molybdate distribution and usage. KO phenotype-analyses, cellular and organ-specific localization, and connection to Moco-biosynthesis enzymes via protein-protein interaction suggest involvement in cellular import of molybdate in leaves and reproductive organs. Furthermore, we detected a glutathione-molybdate complex, which reveals how vacuolar storage is maintained. A putative Golgi S-adenosyl-methionine transport function was reported recently for the MOT2-family. Here, we propose a moonlighting function, since clear evidence of molybdate transport was found in a yeast-system. Our characterization of the MOT2-family and the detection of a glutathione-molybdate complex unveil the plant-wide way of molybdate.
MeSH term(s)	Arabidopsis/genetics ; Arabidopsis/metabolism ; Molybdenum/metabolism ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Pteridines ; Homeostasis
Chemical Substances	molybdate (14259-85-9) ; Molybdenum (81AH48963U) ; Membrane Transport Proteins ; Pteridines
Language	English
Publishing date	2023-08-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-3642
ISSN (online)	2399-3642
DOI	10.1038/s42003-023-05161-x
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Article ; Online: Citizen science shows that small agricultural streams in Germany are in a poor ecological status.

von Gönner, Julia / Gröning, Jonas / Grescho, Volker / Neuer, Lilian / Gottfried, Benjamin / Hänsch, Veit G / Molsberger-Lange, Eva / Wilharm, Elke / Liess, Matthias / Bonn, Aletta

The Science of the total environment

2024 Volume 922, Page(s) 171183

Abstract: Agricultural pesticides, nutrients, and habitat degradation are major causes of insect declines in lowland streams. To effectively conserve and restore stream habitats, standardized stream monitoring data and societal support for freshwater protection ... ...

Abstract	Agricultural pesticides, nutrients, and habitat degradation are major causes of insect declines in lowland streams. To effectively conserve and restore stream habitats, standardized stream monitoring data and societal support for freshwater protection are needed. Here, we sampled 137 small stream monitoring sites across Germany, 83 % of which were located in agricultural catchments, with >900 citizen scientists in 96 monitoring groups. Sampling was carried out according to Water Framework Directive standards as part of the citizen science freshwater monitoring program FLOW in spring and summer 2021, 2022 and 2023. The biological indicator SPEAR
MeSH term(s)	Animals ; Invertebrates ; Ecosystem ; Rivers ; Citizen Science ; Environmental Monitoring/methods ; Water Pollutants, Chemical/analysis ; Pesticides/analysis ; Germany ; Water
Chemical Substances	Water Pollutants, Chemical ; Pesticides ; Water (059QF0KO0R)
Language	English
Publishing date	2024-02-24
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.171183
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Zs.A 949: Show issues

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Article ; Online: Pathophysiologie der implantatassoziierten Infektion : Vom Biofilm zur Osteolyse und septischen Lockerung.

Wagner, C / Hänsch, G M

Der Orthopade

2015 Volume 44, Issue 12, Page(s) 967–973

Abstract: Biofilm formation is the key factor in the pathogenesis of implant-associated infections. The most common pathogens isolated are Staphylococcus species, opportunists belonging to the physiological flora of the skin. Biofilm formation starts with the ... ...

Title translation	Pathophysiology of implant-associated infections: From biofilm to osteolysis and septic loosening.
Abstract	Biofilm formation is the key factor in the pathogenesis of implant-associated infections. The most common pathogens isolated are Staphylococcus species, opportunists belonging to the physiological flora of the skin. Biofilm formation starts with the adhesion of bacteria and colonisation preferentially occurs on the surfaces of the foreign body material. As an interactive symbiotic "city of microbes," biofilm formation represents an efficient survival strategy for bacteria. In clinically apparent infections the biofilm induces a local host response with infiltration of phagocytic immune cells. The proinflammatory microenvironment results in a stimulation of osteoclastogenesis, with local osteolysis, and finally septic loosening of the implant. According to the biofilm theory, retaining the implant in primary revision surgery is only recommended in early-stage infections with a stable implant; in late-stage infections, or when loosening occurs, the implant should be removed. Results of previous anti-biofilm therapies have not been satisfactory; therefore, current research is focused on prevention strategies, especially the modification of implant surfaces. Basic knowledge of the underlying pathophysiology is a prerequisite for the development of innovative interdisciplinary therapy and prevention strategies; in this context, essential aspects of biofilm formation, its consequences, and its relevance to diagnosis and therapy are described and discussed.
MeSH term(s)	Bacterial Infections/etiology ; Bacterial Infections/microbiology ; Bacterial Infections/physiopathology ; Biofilms/growth & development ; Humans ; Osteolysis/complications ; Osteolysis/microbiology ; Osteolysis/physiopathology ; Prosthesis Failure ; Prosthesis-Related Infections/etiology ; Prosthesis-Related Infections/microbiology ; Prosthesis-Related Infections/physiopathology
Language	German
Publishing date	2015-12
Publishing country	Germany
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	121792-6
ISSN	1433-0431 ; 0085-4530
ISSN (online)	1433-0431
ISSN	0085-4530
DOI	10.1007/s00132-015-3183-z
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Zs.A 822: Show issues

Location:
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bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Chimeric antigen receptor T cell-based targeting of CD317 as a novel immunotherapeutic strategy against glioblastoma.

Hänsch, Lena / Peipp, Matthias / Mastall, Maximilian / Villars, Danielle / Myburgh, Renier / Silginer, Manuela / Weiss, Tobias / Gramatzki, Dorothee / Vasella, Flavio / Manz, Markus G / Weller, Michael / Roth, Patrick

Neuro-oncology

2023 Volume 25, Issue 11, Page(s) 2001–2014

Abstract: Background: Chimeric antigen receptor (CAR) T cell therapy has proven to be successful against hematological malignancies. However, exploiting CAR T cells to treat solid tumors is more challenging for various reasons including the lack of suitable ... ...

Abstract	Background: Chimeric antigen receptor (CAR) T cell therapy has proven to be successful against hematological malignancies. However, exploiting CAR T cells to treat solid tumors is more challenging for various reasons including the lack of suitable target antigens. Here, we identify the transmembrane protein CD317 as a novel target antigen for CAR T cell therapy against glioblastoma, one of the most aggressive solid tumors. Methods: CD317-targeting CAR T cells were generated by lentivirally transducing human T cells from healthy donors. The anti-glioma activity of CD317-CAR T cells toward various glioma cells was assessed in vitro in cell lysis assays. Subsequently, we determined the efficacy of CD317-CAR T cells to control tumor growth in vivo in clinically relevant mouse glioma models. Results: We generated CD317-specific CAR T cells and demonstrate strong anti-tumor activity against several glioma cell lines as well as primary patient-derived cells with varying CD317 expression levels in vitro. A CRISPR/Cas9-mediated knockout of CD317 protected glioma cells from CAR T cell lysis, demonstrating the target specificity of the approach. Silencing of CD317 expression in T cells by RNA interference reduced fratricide of engineered T cells and further improved their effector function. Using orthotopic glioma mouse models, we demonstrate the antigen-specific anti-tumor activity of CD317-CAR T cells, which resulted in prolonged survival and cure of a fraction of CAR T cell-treated animals. Conclusions: These data reveal a promising role of CD317-CAR T cell therapy against glioblastoma, which warrants further evaluation to translate this immunotherapeutic strategy into clinical neuro-oncology.
MeSH term(s)	Mice ; Animals ; Humans ; Receptors, Chimeric Antigen/genetics ; Glioblastoma/pathology ; T-Lymphocytes ; Cell Line, Tumor ; Immunotherapy, Adoptive/methods ; Glioma/pathology ; Xenograft Model Antitumor Assays
Chemical Substances	Receptors, Chimeric Antigen
Language	English
Publishing date	2023-05-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2028601-6
ISSN	1523-5866 ; 1522-8517
ISSN (online)	1523-5866
ISSN	1522-8517
DOI	10.1093/neuonc/noad108
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Zs.A 5307: Show issues

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Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

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Article ; Online: Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the human host cell.

Erenburg, Isabelle N / Hänsch, Sebastian / Chacko, Feby M / Hamacher, Anna / Wintgens, Sebastian / Stuhldreier, Fabian / Poschmann, Gereon / Spitz, Olivia / Stühler, Kai / Wesselborg, Sebastian / Hegemann, Johannes H / Smits, Sander H J / Weidtkamp-Peters, Stefanie / Schmitt, Lutz

Scientific reports

2022 Volume 12, Issue 1, Page(s) 17825

Abstract: Many proteins of the Repeats in Toxins (RTX) protein family are toxins of Gram-negative pathogens including hemolysin A (HlyA) of uropathogenic E. coli. RTX proteins are secreted via Type I secretion systems (T1SS) and adopt their native conformation in ... ...

Abstract	Many proteins of the Repeats in Toxins (RTX) protein family are toxins of Gram-negative pathogens including hemolysin A (HlyA) of uropathogenic E. coli. RTX proteins are secreted via Type I secretion systems (T1SS) and adopt their native conformation in the Ca
MeSH term(s)	Humans ; Acyltransferases ; Bacterial Proteins/metabolism ; Escherichia coli/metabolism ; Escherichia coli Proteins/metabolism ; Hemolysin Proteins/metabolism ; Moraxella bovis/metabolism ; Type I Secretion Systems
Chemical Substances	Acyltransferases (EC 2.3.-) ; Bacterial Proteins ; Escherichia coli Proteins ; Hemolysin Proteins ; Type I Secretion Systems
Language	English
Publishing date	2022-10-24
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-22480-x
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Article ; Online: Efficient Gene Delivery of Tailored Amphiphilic Polypeptides by Polyplex Surfing.

Klemm, Paul / Solomun, Jana I / Rodewald, Marko / Kuchenbrod, Maren T / Hänsch, Veit G / Richter, Friederike / Popp, Jürgen / Hertweck, Christian / Hoeppener, Stephanie / Bonduelle, Colin / Lecommandoux, Sébastien / Traeger, Anja / Schubert, Stephanie

Biomacromolecules

2022 Volume 23, Issue 11, Page(s) 4718–4733

Abstract: Within this study, an amphiphilic and potentially biodegradable polypeptide library based on poly[(4-aminobutyl)-l-glutamine- ...

Abstract	Within this study, an amphiphilic and potentially biodegradable polypeptide library based on poly[(4-aminobutyl)-l-glutamine-
MeSH term(s)	Glutamine ; Gene Transfer Techniques ; Genetic Therapy ; Transfection ; Cations ; Peptides
Chemical Substances	Glutamine (0RH81L854J) ; Cations ; Peptides
Language	English
Publishing date	2022-10-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1526-4602
ISSN (online)	1526-4602
DOI	10.1021/acs.biomac.2c00919
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Article ; Online: Enzyme-Primed Native Chemical Ligation Produces Autoinducing Cyclopeptides in Clostridia.

Molloy, Evelyn M / Dell, Maria / Hänsch, Veit G / Dunbar, Kyle L / Feldmann, Romy / Oberheide, Ansgar / Seyfarth, Lydia / Kumpfmüller, Jana / Horch, Therese / Arndt, Hans-Dieter / Hertweck, Christian

Angewandte Chemie (International ed. in English)

2021 Volume 60, Issue 19, Page(s) 10670–10679

Abstract: Clostridia coordinate many important processes such as toxin production, infection, and survival by density-dependent communication (quorum sensing) using autoinducing peptides (AIPs). Although clostridial AIPs have been proposed to be (thio)lactone- ... ...

Abstract	Clostridia coordinate many important processes such as toxin production, infection, and survival by density-dependent communication (quorum sensing) using autoinducing peptides (AIPs). Although clostridial AIPs have been proposed to be (thio)lactone-containing peptides, their true structures remain elusive. Here, we report the genome-guided discovery of an AIP that controls endospore formation in Ruminiclostridium cellulolyticum. Through a combination of chemical synthesis and chemical complementation assays with a mutant strain, we reveal that the genuine chemical mediator is a homodetic cyclopeptide (cAIP). Kinetic analyses indicate that the mature cAIP is produced via a cryptic thiolactone intermediate that undergoes a rapid S→N acyl shift, in a manner similar to intramolecular native chemical ligation (NCL). Finally, by implementing a chemical probe in a targeted screen, we show that this novel enzyme-primed, intramolecular NCL is a widespread feature of clostridial AIP biosynthesis.
MeSH term(s)	Clostridium/chemistry ; Kinetics ; Peptide Hydrolases/chemistry ; Peptide Hydrolases/metabolism ; Peptides, Cyclic/biosynthesis ; Peptides, Cyclic/chemistry
Chemical Substances	Peptides, Cyclic ; Peptide Hydrolases (EC 3.4.-)
Language	English
Publishing date	2021-03-26
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2011836-3
ISSN	1521-3773 ; 1433-7851
ISSN (online)	1521-3773
ISSN	1433-7851
DOI	10.1002/anie.202016378
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Article ; Online: Prophylaxis of implant-related infections by local release of vancomycin from a hydrogel in rabbits.

Boot, W / Vogely, H C / Jiao, C / Nikkels, P G / Pouran, B / van Rijen, M H / Ekkelenkamp, M B / Hänsch, G M / Dhert, W J / Gawlitta, D

European cells & materials

2020 Volume 39, Page(s) 108–120

Abstract: Local prophylaxis with antibiotic-loaded bone cement is a successful method to prevent post-operative infections in patients receiving orthopaedic implants. No comparable method is available for uncemented implants. Therefore, a hydrogel consisting of ... ...

Abstract	Local prophylaxis with antibiotic-loaded bone cement is a successful method to prevent post-operative infections in patients receiving orthopaedic implants. No comparable method is available for uncemented implants. Therefore, a hydrogel consisting of hyaluronic and polylactic acids was evaluated in a rabbit model for delivery of antimicrobial agents to prevent post-operative infections. In a pilot study, the suitability of the in vivo model was assessed by testing the hydrogel as carrier material for antimicrobial agents.In the main study, the antimicrobial-agent-loaded hydrogel was evaluated for infection prophylaxis. Rabbits received a titanium rod intramedullary in the tibia after contamination with Staphylococcus aureus. The rods were coated with unloaded hydrogel (Gel), hydrogel loaded with 2 % (Van2) or 5 % vancomycin (Van5), bioactive glass (BAG) or N-acetyl-L-cysteine (NAC). To analyse the infection severity after 28 d, histopathological, bacteriological, micro-computed tomographic and haematological analyses were performed. In the pilot study, the Van5 group had less infection (0/6 infected) as compared to the Gel group (5/5, p = 0.000) and the in vivo model was deemed suitable. In the main study, in the Van2 and Van5 groups, the number of infected animals was lower [1/6 (p = 0.006) and 2/6 (p = 0.044) infected, respectively]. In contrast, BAG and NAC groups showed no infection reduction (5/6 both groups, p = 0.997). The hydrogel can be used as a local carrier of vancomycin for prophylaxis of implant-related infections.The present study showed promising results for local delivery of antibacterial agents by hydrogel to prevent implant-related infections.
MeSH term(s)	Animals ; Bone and Bones/pathology ; Drug Liberation ; Female ; Hydrogels/chemistry ; Pilot Projects ; Prosthesis-Related Infections/blood ; Prosthesis-Related Infections/drug therapy ; Prosthesis-Related Infections/microbiology ; Prosthesis-Related Infections/prevention & control ; Rabbits ; Titanium ; Vancomycin/therapeutic use ; X-Ray Microtomography
Chemical Substances	Hydrogels ; Vancomycin (6Q205EH1VU) ; Titanium (D1JT611TNE)
Language	English
Publishing date	2020-02-19
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2046669-9
ISSN	1473-2262 ; 1473-2262
ISSN (online)	1473-2262
ISSN	1473-2262
DOI	10.22203/eCM.v039a07
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Article ; Online: Dual-comb coherent Raman spectroscopy with lasers of 1-GHz pulse repetition frequency.

Mohler, Kathrin J / Bohn, Bernhard J / Yan, Ming / Mélen, Gwénaëlle / Hänsch, Theodor W / Picqué, Nathalie

Optics letters

2017 Volume 42, Issue 2, Page(s) 318–321

Abstract: We extend the technique of multiplex coherent Raman spectroscopy with two femtosecond mode-locked lasers to oscillators of a pulse repetition frequency of 1 GHz. We demonstrate a spectra of liquids, which span 1100 cm ... -1 ... of Raman shifts. At a ... ...

Abstract	We extend the technique of multiplex coherent Raman spectroscopy with two femtosecond mode-locked lasers to oscillators of a pulse repetition frequency of 1 GHz. We demonstrate a spectra of liquids, which span 1100 cm<sup>-1</sup> of Raman shifts. At a resolution of 6 cm<sup>-1</sup>, their measurement time may be as short as 5 μs for a refresh rate of 2 kHz. The waiting period between acquisitions is improved 10-fold compared to previous experiments with two lasers of 100-MHz repetition frequencies.
Language	English
Publishing date	2017-01-15
Publishing country	United States
Document type	Journal Article
ISSN	1539-4794
ISSN (online)	1539-4794
DOI	10.1364/OL.42.000318
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Article ; Online: Mid-infrared dual-comb spectroscopy with electro-optic modulators.

Yan, Ming / Luo, Pei-Ling / Iwakuni, Kana / Millot, Guy / Hänsch, Theodor W / Picqué, Nathalie

Light, science & applications

2017 Volume 6, Issue 10, Page(s) e17076

Abstract: Absorption spectroscopy of fundamental ro-vibrational transitions in the mid-infrared region provides a powerful tool for studying the structure and dynamics of molecules in the gas phase and for sensitive and quantitative gas sensing. Laser frequency ... ...

Abstract	Absorption spectroscopy of fundamental ro-vibrational transitions in the mid-infrared region provides a powerful tool for studying the structure and dynamics of molecules in the gas phase and for sensitive and quantitative gas sensing. Laser frequency combs permit novel approaches to perform broadband molecular spectroscopy. Multiplex dual-comb spectroscopy without moving parts can achieve particularly high speed, sensitivity and resolution. However, achieving Doppler-limited resolution in the mid-infrared still requires overcoming instrumental challenges. Here we demonstrate a new approach based on difference-frequency generation of frequency-agile near-infrared frequency combs that are produced using electro-optic modulators. The combs have a remarkably flat intensity distribution, and their positions and line spacings can be freely selected by simply dialing a knob. Using the proposed technique, we record, in the 3-μm region, Doppler-limited absorption spectra with resolved comb lines within milliseconds, and precise molecular line parameters are retrieved. Our technique holds promise for fast and sensitive time-resolved studies of, for example, trace gases.
Language	English
Publishing date	2017-10-20
Publishing country	England
Document type	Journal Article
ZDB-ID	2662628-7
ISSN	2047-7538 ; 2047-7538
ISSN (online)	2047-7538
ISSN	2047-7538
DOI	10.1038/lsa.2017.76
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