LIVIVO - Search results -

Search results

Result 1 - 10 of total 5095

Search options

Article ; Online: Spectrum of K

Kang, Seok Kyu / Vanoye, Carlos G / Misra, Sunita N / Echevarria, Dennis M / Calhoun, Jeffrey D / O'Connor, John B / Fabre, Katarina L / McKnight, Dianalee / Demmer, Laurie / Goldenberg, Paula / Grote, Lauren E / Thiffault, Isabelle / Saunders, Carol / Strauss, Kevin A / Torkamani, Ali / van der Smagt, Jasper / van Gassen, Koen / Carson, Robert P / Diaz, Jullianne /

Leon, Eyby / Jacher, Joseph E / Hannibal, Mark C / Litwin, Jessica / Friedman, Neil R / Schreiber, Allison / Lynch, Bryan / Poduri, Annapurna / Marsh, Eric D / Goldberg, Ethan M / Millichap, John J / George, Alfred L / Kearney, Jennifer A

Annals of neurology

2019 Volume 86, Issue 6, Page(s) 899–912

Abstract: Objective: Pathogenic variants in KCNB1, encoding the voltage-gated potassium channel K: Methods ... we investigated the biophysical properties and cell-surface expression of variant K: Results: Pathogenic ... dependence of activation and/or inactivation, as homotetramers or when coexpressed with wild-type K ...

Abstract	Objective: Pathogenic variants in KCNB1, encoding the voltage-gated potassium channel K Methods: We evaluated a series of 17 KCNB1 variants associated with DEE or other neurodevelopmental disorders (NDDs) to rapidly ascertain channel dysfunction using high-throughput functional assays. Specifically, we investigated the biophysical properties and cell-surface expression of variant K Results: Pathogenic variants exhibited diverse functional defects, including altered current density and shifts in the voltage dependence of activation and/or inactivation, as homotetramers or when coexpressed with wild-type K Interpretation: Our study establishes a platform for rapid screening of K
MeSH term(s)	Amino Acid Sequence ; Animals ; CHO Cells ; Cricetinae ; Cricetulus ; Genetic Variation/genetics ; High-Throughput Screening Assays/methods ; Humans ; Neurodevelopmental Disorders/diagnosis ; Neurodevelopmental Disorders/genetics ; Protein Structure, Secondary ; Shab Potassium Channels/chemistry ; Shab Potassium Channels/genetics
Chemical Substances	KCNB1 protein, human ; Shab Potassium Channels
Language	English
Publishing date	2019-10-24
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	80362-5
ISSN	1531-8249 ; 0364-5134
ISSN (online)	1531-8249
ISSN	0364-5134
DOI	10.1002/ana.25607
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1370: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 478: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Comparison of Traumatic Intracranial Hemorrhage Expansion and Outcomes Among Patients on Direct Oral Anticoagulants Versus Vitamin k Antagonists.

Shin, Samuel S / Marsh, Elisabeth B / Ali, Hasan / Nyquist, Paul A / Hanley, Daniel F / Ziai, Wendy C

Neurocritical care

2020 Volume 32, Issue 2, Page(s) 407–418

Abstract: ... with vitamin k antagonists (VKA: warfarin) and DOACs (apixaban, rivaroxaban, dabigatran).: Methods ...

Abstract	Background: With increasing use of direct oral anticoagulants (DOACs) and availability of new reversal agents, the risk of traumatic intracranial hemorrhage (tICH) requires better understanding. We compared hemorrhage expansion rates, mortality, and morbidity following tICH in patients treated with vitamin k antagonists (VKA: warfarin) and DOACs (apixaban, rivaroxaban, dabigatran). Methods: Retrospective chart review of patients from 2010 to 2017 was performed to identify patients with imaging diagnosis of acute traumatic intraparenchymal, subdural, subarachnoid, and epidural hemorrhage with preadmission use of DOACs or VKAs. We identified 39 patients on DOACs and 97 patients on VKAs. Demographic information, comorbidities, hemorrhage size, and expansion over time, as well as discharge disposition and Glasgow Outcome Scale (GOS) were collected. Primary outcome was development of new or enlargement of tICH within the first 48 h of initial CT imaging. Results: Of 136 patients with mean (SD) age 78.7 (13.2) years, most common tICH subtype was subdural hematoma (N = 102/136; 75%), and most common mechanism was a fall (N = 130/136; 95.6%). Majority of patients in the DOAC group did not receive reversal agents (66.7%). Hemorrhage expansion or new hemorrhage occurred in 11.1% in DOAC group vs. 14.6% in VKA group (p = 0.77) at a median of 8 and 11 h from initial ED admission, respectively (p = 0.82). Patients in the DOAC group compared to VKA group had higher median discharge GOS (4 vs. 3 respectively, p = 0.03), higher percentage of patients with good outcome (GOS 4-5, 66.7% vs. 40.2% respectively, p = 0.005), and higher rate of discharge to home or rehabilitation (p = 0.04). Conclusions: We report anticoagulation-associated tICH outcomes predominantly due to fall-related subdural hematomas. Patients on DOACs had lower tICH expansion rates although not statistically significantly different from VKA-treated patients. DOAC-treated patients had favorable outcomes versus VKA group following tICH despite low use of reversal strategies. DOAC use may be a safer alternative to VKA in patients at risk of traumatic brain hemorrhage.
MeSH term(s)	Accidental Falls ; Aged ; Aged, 80 and over ; Anticoagulants/adverse effects ; Antifibrinolytic Agents/therapeutic use ; Antithrombins/adverse effects ; Blood Coagulation Factors/therapeutic use ; Coagulants/therapeutic use ; Dabigatran/adverse effects ; Disease Progression ; Factor Xa Inhibitors/adverse effects ; Female ; Glasgow Outcome Scale ; Humans ; Intracranial Hemorrhage, Traumatic/chemically induced ; Intracranial Hemorrhage, Traumatic/physiopathology ; Intracranial Hemorrhage, Traumatic/therapy ; Length of Stay ; Male ; Middle Aged ; Mortality ; Neurosurgical Procedures ; Plasma ; Platelet Transfusion ; Pyrazoles/adverse effects ; Pyridines/adverse effects ; Pyridones/adverse effects ; Retrospective Studies ; Rivaroxaban/adverse effects ; Thiazoles/adverse effects ; Vitamin K/therapeutic use ; Warfarin/adverse effects
Chemical Substances	Anticoagulants ; Antifibrinolytic Agents ; Antithrombins ; Blood Coagulation Factors ; Coagulants ; Factor Xa Inhibitors ; Pyrazoles ; Pyridines ; Pyridones ; Thiazoles ; Vitamin K (12001-79-5) ; prothrombin complex concentrates (37224-63-8) ; apixaban (3Z9Y7UWC1J) ; Warfarin (5Q7ZVV76EI) ; Rivaroxaban (9NDF7JZ4M3) ; anti-inhibitor coagulant complex (CS849DUN3M) ; Dabigatran (I0VM4M70GC) ; edoxaban (NDU3J18APO)
Language	English
Publishing date	2020-02-07
Publishing country	United States
Document type	Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural
ZDB-ID	2381896-7
ISSN	1556-0961 ; 1541-6933
ISSN (online)	1556-0961
ISSN	1541-6933
DOI	10.1007/s12028-019-00898-y
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 6538: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Lipid Librations at the Interface with the Na,K-ATPase.

Guzzi, Rita / Bartucci, Rosa / Esmann, Mikael / Marsh, Derek

Biophysical journal

2015 Volume 108, Issue 12, Page(s) 2825–2832

Abstract: ... librational motion of spin-labeled lipid chains in membranous Na,K-ATPase is investigated by spin-echo ... spin-labeled fatty acids that display selectivity of interaction with the Na,K-ATPase. Echo-detected electron ...

Abstract	Transitions between conformational substates of membrane proteins can be driven by torsional librations in the protein that may be coupled to librational fluctuations of the lipid chains. Here, librational motion of spin-labeled lipid chains in membranous Na,K-ATPase is investigated by spin-echo electron paramagnetic resonance. Lipids at the protein interface are targeted by using negatively charged spin-labeled fatty acids that display selectivity of interaction with the Na,K-ATPase. Echo-detected electron paramagnetic resonance spectra from native membranes are corrected for the contribution from the bilayer regions of the membrane by using spectra from dispersions of the extracted membrane lipids. Lipid librations at the protein interface have a flat profile with chain position, whereas librational fluctuations of the bilayer lipids increase pronouncedly from C-9 onward, then flatten off toward the terminal methyl end of the chains. This difference is accounted for by increased torsional amplitude at the chain ends in bilayers, while the amplitude remains restricted throughout the chain at the protein interface with a limited lengthening in correlation time. The temperature dependence of chain librations at the protein interface strongly resembles that of the spin-labeled protein side chains, suggesting solvent-mediated transitions in the protein are driven by fluctuations in the lipid environment.
MeSH term(s)	Fatty Acids/chemistry ; Lipid Bilayers/chemistry ; Sodium-Potassium-Exchanging ATPase/chemistry ; Torsion, Mechanical
Chemical Substances	Fatty Acids ; Lipid Bilayers ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)
Language	English
Publishing date	2015-06-16
Publishing country	United States
Document type	Journal Article
ZDB-ID	218078-9
ISSN	1542-0086 ; 0006-3495
ISSN (online)	1542-0086
ISSN	0006-3495
DOI	10.1016/j.bpj.2015.05.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

In stock of ZB MED Cologne/Königswinter

Zs.A 235: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 2: Show issues

Order via subito

Details ▾

Article ; Online: A G₄·K⁺ hydrogel stabilized by an anion.

Peters, Gretchen Marie / Skala, Luke P / Plank, Taylor N / Hyman, Brooke J / Manjunatha Reddy, G N / Marsh, Andrew / Brown, Steven P / Davis, Jeffery T

Journal of the American Chemical Society

2014 Volume 136, Issue 36, Page(s) 12596–12599

Abstract: ... as it links two molecules of 1, which facilitates cation-templated assembly of G4·K(+) quartets. The guanosine ... relevant concentrations of K(+). Furthermore, non-covalent interactions, such as electrostatics, π-stacking ...

Abstract	Supramolecular hydrogels derived from natural products have promising applications in diagnostics, drug delivery, and tissue engineering. We studied the formation of a long-lived hydrogel made by mixing guanosine (G, 1) with 0.5 equiv of KB(OH)4. This ratio of borate anion to ligand is crucial for gelation as it links two molecules of 1, which facilitates cation-templated assembly of G4·K(+) quartets. The guanosine-borate (GB) hydrogel, which was characterized by cryogenic transmission electron microscopy and circular dichroism and (11)B magic-angle-spinning NMR spectroscopy, is stable in water that contains physiologically relevant concentrations of K(+). Furthermore, non-covalent interactions, such as electrostatics, π-stacking, and hydrogen bonding, enable the incorporation of a cationic dye and nucleosides into the GB hydrogel.
MeSH term(s)	Anions/chemistry ; Borates/chemistry ; Guanosine/chemistry ; Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry ; Magnetic Resonance Spectroscopy ; Molecular Structure ; Potassium/chemistry
Chemical Substances	Anions ; Borates ; Guanosine (12133JR80S) ; Hydrogel, Polyethylene Glycol Dimethacrylate (25852-47-5) ; Potassium (RWP5GA015D)
Language	English
Publishing date	2014-09-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021/ja507506c
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 4' 55/32: Show issues
Z 7.3: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Psychometric Validation of the Parental Bonding Instrument in a U.K. Population-Based Sample: Role of Gender and Association With Mental Health in Mid-Late Life.

Xu, Man K / Morin, Alexandre J S / Marsh, Herbert W / Richards, Marcus / Jones, Peter B

Assessment

2016 Volume 25, Issue 6, Page(s) 716–728

Abstract: The factorial structure of the Parental Bonding Instrument (PBI) has been frequently studied in diverse samples but no study has examined its psychometric properties from large, population-based samples. In particular, important questions have not been ... ...

Abstract	The factorial structure of the Parental Bonding Instrument (PBI) has been frequently studied in diverse samples but no study has examined its psychometric properties from large, population-based samples. In particular, important questions have not been addressed such as the measurement invariance properties across parental and offspring gender. We evaluated the PBI based on responses from a large, representative population-based sample, using an exploratory structural equation modeling method appropriate for categorical data. Analysis revealed a three-factor structure representing "care," "overprotection," and "autonomy" parenting styles. In terms of psychometric measurement validity, our results supported the complete invariance of the PBI ratings across sons and daughters for their mothers and fathers. The PBI ratings were also robust in relation to personality and mental health status. In terms of predictive value, paternal care showed a protective effect on mental health at age 43 in sons. The PBI is a sound instrument for capturing perceived parenting styles, and is predictive of mental health in middle adulthood.
MeSH term(s)	Adult ; Female ; Humans ; Male ; Mental Health ; Object Attachment ; Parent-Child Relations ; Parenting/psychology ; Psychometrics ; Surveys and Questionnaires ; United Kingdom
Language	English
Publishing date	2016-08-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
ZDB-ID	1362144-0
ISSN	1552-3489 ; 1073-1911
ISSN (online)	1552-3489
ISSN	1073-1911
DOI	10.1177/1073191116660813
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 4650: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Water penetration profile at the protein-lipid interface in Na,K-ATPase membranes.

Bartucci, Rosa / Guzzi, Rita / Esmann, Mikael / Marsh, Derek

Biophysical journal

2014 Volume 107, Issue 6, Page(s) 1375–1382

Abstract: The affinity of ionized fatty acids for the Na,K-ATPase is used to determine the transmembrane ... for stearic acid, n-SASL, spin-labeled systematically at the C-n atoms throughout the chain. In both native Na,K ... in the lipid bilayers, a much broader transition region in the range n = 6 to 10 is found with Na,K-ATPase membranes ...

Abstract	The affinity of ionized fatty acids for the Na,K-ATPase is used to determine the transmembrane profile of water penetration at the protein-lipid interface. The standardized intensity of the electron spin echo envelope modulation (ESEEM) from (2)H-hyperfine interaction with D2O is determined for stearic acid, n-SASL, spin-labeled systematically at the C-n atoms throughout the chain. In both native Na,K-ATPase membranes from shark salt gland and bilayers of the extracted membrane lipids, the D2O-ESEEM intensities of fully charged n-SASL decrease progressively with position down the fatty acid chain toward the terminal methyl group. Whereas the D2O intensities decrease sharply at the n = 9 position in the lipid bilayers, a much broader transition region in the range n = 6 to 10 is found with Na,K-ATPase membranes. Correction for the bilayer population in the membranes yields the intrinsic D2O-intensity profile at the protein-lipid interface. For positions at either end of the chains, the D2O concentrations at the protein interface are greater than in the lipid bilayer, and the positional profile is much broader. This reveals the higher polarity, and consequently higher intramembrane water concentration, at the protein-lipid interface. In particular, there is a significant water concentration adjacent to the protein at the membrane midplane, unlike the situation in the bilayer regions of this cholesterol-rich membrane. Experiments with protonated fatty acid and phosphatidylcholine spin labels, both of which have a considerably lower affinity for the Na,K-ATPase, confirm these results.
MeSH term(s)	Animals ; Cell Membrane/metabolism ; Cell Membrane Permeability ; Fatty Acids/metabolism ; Membrane Lipids/metabolism ; Models, Molecular ; Phosphatidylcholines/metabolism ; Protein Conformation ; Protons ; Sodium-Potassium-Exchanging ATPase/chemistry ; Sodium-Potassium-Exchanging ATPase/metabolism ; Spin Labels ; Water/metabolism
Chemical Substances	Fatty Acids ; Membrane Lipids ; Phosphatidylcholines ; Protons ; Spin Labels ; Water (059QF0KO0R) ; Sodium-Potassium-Exchanging ATPase (EC 7.2.2.13)
Language	English
Publishing date	2014-10-14
Publishing country	United States
Document type	Journal Article
ZDB-ID	218078-9
ISSN	1542-0086 ; 0006-3495
ISSN (online)	1542-0086
ISSN	0006-3495
DOI	10.1016/j.bpj.2014.07.057
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 235: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 2: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A G4Â·K+ Hydrogel Stabilized by an Anion

Peters, Gretchen Marie / Brown Steven P / Davis Jeffery T / Hyman Brooke J / Manjunatha Reddy G. N / Marsh Andrew / Plank Taylor N / Skala Luke P

Journal of the American Chemical Society. 2014 Sept. 10, v. 136, no. 36

2014

Abstract: Supramolecular hydrogels derived from natural products have promising applications in diagnostics, drug delivery, and tissue engineering. We studied the formation of a long-lived hydrogel made by mixing guanosine (G, 1) with 0.5 equiv of KB(OH)â. This ... ...

Abstract	Supramolecular hydrogels derived from natural products have promising applications in diagnostics, drug delivery, and tissue engineering. We studied the formation of a long-lived hydrogel made by mixing guanosine (G, 1) with 0.5 equiv of KB(OH)â. This ratio of borate anion to ligand is crucial for gelation as it links two molecules of 1, which facilitates cation-templated assembly of GâÂ·Kâº quartets. The guanosineâborate (GB) hydrogel, which was characterized by cryogenic transmission electron microscopy and circular dichroism and Â¹Â¹B magic-angle-spinning NMR spectroscopy, is stable in water that contains physiologically relevant concentrations of Kâº. Furthermore, non-covalent interactions, such as electrostatics, Ï-stacking, and hydrogen bonding, enable the incorporation of a cationic dye and nucleosides into the GB hydrogel.
Keywords	borates ; circular dichroism spectroscopy ; diagnostic techniques ; drugs ; gelation ; guanosine ; hydrogels ; hydrogen bonding ; ligands ; mixing ; nuclear magnetic resonance spectroscopy ; potassium ; tissue engineering ; transmission electron microscopy
Language	English
Dates of publication	2014-0910
Size	p. 12596-12599.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	3155-0
ISSN	1520-5126 ; 0002-7863
ISSN (online)	1520-5126
ISSN	0002-7863
DOI	10.1021%2Fja507506c
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 4' 55/32: Show issues
Z 7.3: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article: Indanedione spin labelling of Na,K-ATPase.

Esmann, Mikael / Sár, Cecilia P / Hideg, Kálmán / Marsh, Derek

Biochimica et biophysica acta

2002 Volume 1567, Issue 1-2, Page(s) 34–40

Abstract: ... of membranous Na,K-ATPase from Squalus acanthias. With a conjugated diene spacer, the majority of spin labels ... Electrostatic repulsion contributes to the lateral interactions between Na,K-ATPase molecules. ...

Abstract	The indanedione series of vinyl ketone spin-labelling reagents has been extended in two ways: by increasing the length of the rigid spacer between the reactive centre and the nitroxide ring, or by introducing an electrophilic substituent (that could also hinder its rotation) at the bridge head position of the nitroxide ring. Three reagents of this new series have been used to spin label the Class II thiol groups of membranous Na,K-ATPase from Squalus acanthias. With a conjugated diene spacer, the majority of spin labels are strongly held but a minor population is relatively mobile at 37 degrees C. With a conjugated triene spacer, the nitroxide is still strongly held but a portion of the label is non-covalently bound. The 4-bromo-pyrroline derivative (with short vinyl spacer) is tightly held at the attachment site, and the conventional electron paramagnetic resonance (EPR) spectra distinguish between the two enantiomeric structures which differ in their mobility at 37 degrees C. Saturation transfer EPR (ST-EPR) spectra of this label at 4 degrees C have been used to determine the dependence of the protein rotational mobility on ionic strength. Electrostatic repulsion contributes to the lateral interactions between Na,K-ATPase molecules.
MeSH term(s)	Animals ; Dogfish ; Electron Spin Resonance Spectroscopy ; Indans/chemistry ; Sodium-Potassium-Exchanging ATPase/chemistry ; Spin Labels
Chemical Substances	1,2-indanedione ; Indans ; Spin Labels ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)
Language	English
Publishing date	2002-12-09
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/s0005-2736(02)00546-1
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.247: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Stabilization of Na,K-ATPase by ionic interactions.

Fodor, Elfrieda / Fedosova, Natalya U / Ferencz, Csilla / Marsh, Derek / Pali, Tibor / Esmann, Mikael

Biochimica et biophysica acta

2008 Volume 1778, Issue 4, Page(s) 835–843

Abstract: The effect of ions on the thermostability and unfolding of Na,K-ATPase from shark salt gland was ... studied and compared with that of Na,K-ATPase from pig kidney by using differential scanning calorimetry ... of the two Na,K-ATPases correlate with the respective physiological temperatures, and may be attributed ...

Abstract	The effect of ions on the thermostability and unfolding of Na,K-ATPase from shark salt gland was studied and compared with that of Na,K-ATPase from pig kidney by using differential scanning calorimetry (DSC) and activity assays. In 1 mM histidine at pH 7, the shark enzyme inactivates rapidly at 20 degrees C, as does the kidney enzyme at 42 degrees C (but not at 20 degrees C). Increasing ionic strength by addition of 20 mM histidine, or of 1 mM NaCl or KCl, protects both enzymes against this rapid inactivation. As detected by DSC, the shark enzyme undergoes thermal unfolding at lower temperature (Tm approximately 45 degrees C) than does the kidney enzyme (Tm approximately 55 degrees C). Both calorimetric endotherms indicate multi-step unfolding, probably associated with different cooperative domains. Whereas the overall heat of unfolding is similar for the kidney enzyme in either 1 mM or 20 mM histidine, components with high mid-point temperatures are lost from the unfolding transition of the shark enzyme in 1 mM histidine, relative to that in 20 mM histidine. This is attributed to partial unfolding of the enzyme due to a high hydrostatic pressure during centrifugation of DSC samples at low ionic strength, which correlates with inactivation measurements. Addition of 10 mM NaCl to shark enzyme in 1 mM histidine protects against inactivation during centrifugation of the DSC sample, but incubation for 1 h at 20 degrees C prior to addition of NaCl results in loss of components with lower mid-point temperatures within the unfolding transition. Cations at millimolar concentration therefore afford at least two distinct modes of stabilization, likely affecting separate cooperative domains. The different thermal stabilities and denaturation temperatures of the two Na,K-ATPases correlate with the respective physiological temperatures, and may be attributed to the different lipid environments.
MeSH term(s)	Amino Acid Sequence ; Animals ; Calorimetry, Differential Scanning ; Enzyme Activation/drug effects ; Enzyme Stability/drug effects ; Hot Temperature ; Kidney/enzymology ; Membranes/drug effects ; Membranes/enzymology ; Molecular Sequence Data ; Osmolar Concentration ; Salts/pharmacology ; Sharks ; Sodium-Potassium-Exchanging ATPase/chemistry ; Sodium-Potassium-Exchanging ATPase/metabolism ; Swine
Chemical Substances	Salts ; Sodium-Potassium-Exchanging ATPase (EC 3.6.3.9)
Language	English
Publishing date	2008-04
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbamem.2007.12.006
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.247: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Genomic Epidemiology of an Endoscope-Associated Outbreak of Klebsiella pneumoniae Carbapenemase (KPC)-Producing K. pneumoniae.

Marsh, Jane W / Krauland, Mary G / Nelson, Jemma S / Schlackman, Jessica L / Brooks, Anthony M / Pasculle, A William / Shutt, Kathleen A / Doi, Yohei / Querry, Ashley M / Muto, Carlene A / Harrison, Lee H

PloS one

2015 Volume 10, Issue 12, Page(s) e0144310

Abstract: Increased incidence of infections due to Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) was noted among patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) at a single hospital. An epidemiologic ... ...

Abstract	Increased incidence of infections due to Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) was noted among patients undergoing endoscopic retrograde cholangiopancreatography (ERCP) at a single hospital. An epidemiologic investigation identified KPC-Kp and non-KPC-producing, extended-spectrum β-lactamase (ESBL)-producing Kp in cultures from 2 endoscopes. Genotyping was performed on patient and endoscope isolates to characterize the microbial genomics of the outbreak. Genetic similarity of 51 Kp isolates from 37 patients and 3 endoscopes was assessed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Five patient and 2 endoscope isolates underwent whole genome sequencing (WGS). Two KPC-encoding plasmids were characterized by single molecule, real-time sequencing. Plasmid diversity was assessed by endonuclease digestion. Genomic and epidemiologic data were used in conjunction to investigate the outbreak source. Two clusters of Kp patient isolates were genetically related to endoscope isolates by PFGE. A subset of patient isolates were collected post-ERCP, suggesting ERCP endoscopes as a possible source. A phylogeny of 7 Kp genomes from patient and endoscope isolates supported ERCP as a potential source of transmission. Differences in gene content defined 5 ST258 subclades and identified 2 of the subclades as outbreak-associated. A novel KPC-encoding plasmid, pKp28 helped define and track one endoscope-associated ST258 subclade. WGS demonstrated high genetic relatedness of patient and ERCP endoscope isolates suggesting ERCP-associated transmission of ST258 KPC-Kp. Gene and plasmid content discriminated the outbreak from endemic ST258 populations and assisted with the molecular epidemiologic investigation of an extended KPC-Kp outbreak.
MeSH term(s)	Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics ; Cholangiopancreatography, Endoscopic Retrograde/adverse effects ; Disease Outbreaks ; Female ; Genome, Bacterial ; Humans ; Klebsiella Infections/enzymology ; Klebsiella Infections/epidemiology ; Klebsiella Infections/etiology ; Klebsiella Infections/genetics ; Klebsiella pneumoniae/genetics ; Klebsiella pneumoniae/isolation & purification ; Male ; Phylogeny ; Plasmids/genetics ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
Chemical Substances	Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0144310
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Full text

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- Full text online
- Order with fees

To top

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito

Inter-library loan at ZB MED