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  1. Article ; Online: Author Correction: Designing multiepitope-based vaccine against Eimeria from immune mapped protein 1 (IMP-1) antigen using immunoinformatic approach.

    Madlala, Thabile / Adeleke, Victoria T / Fatoba, Abiodun J / Okpeku, Moses / Adeniyi, Adebayo A / Adeleke, Matthew A

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 15369

    Language English
    Publishing date 2022-09-13
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-20081-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Immunoinformatics prediction of overlapping CD8

    Fatoba, Abiodun J / Maharaj, Leah / Adeleke, Victoria T / Okpeku, Moses / Adeniyi, Adebayo A / Adeleke, Matthew A

    Vaccine

    2021  Volume 39, Issue 7, Page(s) 1111–1121

    Abstract: At the beginning of the year 2020, the world was struck with a global pandemic virus referred to as SARS-CoV-2 (COVID-19) which has left hundreds of thousands of people dead. To control this virus, vaccine design becomes imperative. In this study, ... ...

    Abstract At the beginning of the year 2020, the world was struck with a global pandemic virus referred to as SARS-CoV-2 (COVID-19) which has left hundreds of thousands of people dead. To control this virus, vaccine design becomes imperative. In this study, potential epitopes-based vaccine candidates were explored. Six hundred (600) genomes of SARS-CoV-2 were retrieved from the viPR database to generate CD8
    MeSH term(s) CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; COVID-19/prevention & control ; COVID-19 Vaccines/immunology ; Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/immunology ; Humans ; Interferon-gamma ; Interleukin-4 ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/immunology ; Viral Matrix Proteins/immunology
    Chemical Substances COVID-19 Vaccines ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Spike Glycoprotein, Coronavirus ; Viral Matrix Proteins ; membrane protein, SARS-CoV-2 ; spike protein, SARS-CoV-2 ; Interleukin-4 (207137-56-2) ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2021-01-18
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605674-x
    ISSN 1873-2518 ; 0264-410X
    ISSN (online) 1873-2518
    ISSN 0264-410X
    DOI 10.1016/j.vaccine.2021.01.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 458: Show issues

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  3. Article ; Online: Designing multiepitope-based vaccine against Eimeria from immune mapped protein 1 (IMP-1) antigen using immunoinformatic approach.

    Madlala, Thabile / Adeleke, Victoria T / Fatoba, Abiodun J / Okpeku, Moses / Adeniyi, Adebayo A / Adeleke, Matthew A

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 18295

    Abstract: Drug resistance against coccidiosis has posed a significant threat to chicken welfare and productivity worldwide, putting daunting pressure on the poultry industry to reduce the use of chemoprophylactic drugs and live vaccines in poultry to treat ... ...

    Abstract Drug resistance against coccidiosis has posed a significant threat to chicken welfare and productivity worldwide, putting daunting pressure on the poultry industry to reduce the use of chemoprophylactic drugs and live vaccines in poultry to treat intestinal diseases. Chicken coccidiosis, caused by an apicomplexan parasite of Eimeria spp., is a significant challenge worldwide. Due to the experience of economic loss in production and prevention of the disease, development of cost-effective vaccines or drugs that can stimulate defence against multiple Eimeria species is imperative to control coccidiosis. This study explored Eimeria immune mapped protein-1 (IMP-1) to develop a multiepitope-based vaccine against coccidiosis by identifying antigenic T-cell and B-cell epitope candidates through immunoinformatic techniques. This resulted in the design of 7 CD8
    MeSH term(s) Animals ; Antigens, Protozoan/genetics ; Antigens, Protozoan/immunology ; Chickens/immunology ; Chickens/parasitology ; Coccidiosis/immunology ; Coccidiosis/prevention & control ; Coccidiosis/veterinary ; Conserved Sequence/genetics ; Eimeria/genetics ; Eimeria/immunology ; Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/immunology ; Poultry Diseases/immunology ; Poultry Diseases/parasitology ; Poultry Diseases/prevention & control ; Protozoan Proteins/genetics ; Protozoan Proteins/immunology ; Protozoan Vaccines/immunology
    Chemical Substances Antigens, Protozoan ; Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Protozoan Proteins ; Protozoan Vaccines
    Language English
    Publishing date 2021-09-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-97880-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Immunoinformatics approach for multi-epitope vaccine design against P. falciparum malaria.

    Maharaj, Leah / Adeleke, Victoria T / Fatoba, Abiodun J / Adeniyi, Adebayo A / Tshilwane, Selaelo I / Adeleke, Matthew A / Maharaj, Rajendra / Okpeku, Moses

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases

    2021  Volume 92, Page(s) 104875

    Abstract: Plasmodium falciparum (P. falciparum) is a leading causative agent of malaria, an infectious disease that can be fatal. Unfortunately, control measures are becoming less effective over time. A vaccine is needed to effectively control malaria and lead ... ...

    Abstract Plasmodium falciparum (P. falciparum) is a leading causative agent of malaria, an infectious disease that can be fatal. Unfortunately, control measures are becoming less effective over time. A vaccine is needed to effectively control malaria and lead towards the total elimination of the disease. There have been multiple attempts to develop a vaccine, but to date, none have been certified as appropriate for wide-scale use. In this study, an immunoinformatics method is presented to design a multi-epitope vaccine construct predicted to be effective against P. falciparum malaria. This was done through the prediction of 12 CD4+ T-cell, 10 CD8+ T-cell epitopes and, 1 B-cell epitope which were assessed for predicted high antigenicity, immunogenicity, and non-allergenicity through in silico methods. The Human Leukocyte Antigen (HLA) population coverage showed that the alleles associated with the epitopes accounted for 78.48% of the global population. The CD4+ and CD8+ T-cell epitopes were docked to HLA-DRB1*07:01 and HLA-A*32:01 successfully. Therefore, the epitopes were deemed to be suitable as components of a multi-epitope vaccine construct. Adjuvant RS09 was added to the construct to generate a stronger immune response, as confirmed by an immune system simulation. Finally, the structural stability of the predicted multi-epitope vaccine was assessed using molecular dynamics simulations. The results show a promising vaccine design that should be further synthesised and assessed for its efficacy in an experimental laboratory setting.
    MeSH term(s) Computational Biology ; Epitopes, B-Lymphocyte/immunology ; Epitopes, T-Lymphocyte/immunology ; Humans ; Malaria Vaccines/chemistry ; Malaria, Falciparum/prevention & control ; Plasmodium falciparum/immunology
    Chemical Substances Epitopes, B-Lymphocyte ; Epitopes, T-Lymphocyte ; Malaria Vaccines
    Language English
    Publishing date 2021-04-24
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2037068-4
    ISSN 1567-7257 ; 1567-1348
    ISSN (online) 1567-7257
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2021.104875
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5645: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Designing multiepitope-based vaccine against Eimeria from immune mapped protein 1 (IMP-1) antigen using immunoinformatic approach

    Thabile Madlala / Victoria T. Adeleke / Abiodun J. Fatoba / Moses Okpeku / Adebayo A. Adeniyi / Matthew A. Adeleke

    Scientific Reports, Vol 11, Iss 1, Pp 1-

    2021  Volume 17

    Abstract: Abstract Drug resistance against coccidiosis has posed a significant threat to chicken welfare and productivity worldwide, putting daunting pressure on the poultry industry to reduce the use of chemoprophylactic drugs and live vaccines in poultry to ... ...

    Abstract Abstract Drug resistance against coccidiosis has posed a significant threat to chicken welfare and productivity worldwide, putting daunting pressure on the poultry industry to reduce the use of chemoprophylactic drugs and live vaccines in poultry to treat intestinal diseases. Chicken coccidiosis, caused by an apicomplexan parasite of Eimeria spp., is a significant challenge worldwide. Due to the experience of economic loss in production and prevention of the disease, development of cost-effective vaccines or drugs that can stimulate defence against multiple Eimeria species is imperative to control coccidiosis. This study explored Eimeria immune mapped protein-1 (IMP-1) to develop a multiepitope-based vaccine against coccidiosis by identifying antigenic T-cell and B-cell epitope candidates through immunoinformatic techniques. This resulted in the design of 7 CD8+, 21 CD4+ T-cell epitopes and 6 B-cell epitopes, connected using AAY, GPGPG and KK linkers to form a vaccine construct. A Cholera Toxin B (CTB) adjuvant was attached to the N-terminal of the multiepitope construct to improve the immunogenicity of the vaccine. The designed vaccine was assessed for immunogenicity (8.59968), allergenicity and physiochemical parameters, which revealed the construct molecular weight of 73.25 kDa, theoretical pI of 8.23 and instability index of 33.40. Molecular docking simulation of vaccine with TLR-5 with binding affinity of − 151.893 kcal/mol revealed good structural interaction and stability of protein structure of vaccine construct. The designed vaccine predicts the induction of immunity and boosted host's immune system through production of antibodies and cytokines, vital in hindering surface entry of parasites into host. This is a very important step in vaccine development though further experimental study is still required to validate these results.
    Keywords Medicine ; R ; Science ; Q
    Subject code 570
    Language English
    Publishing date 2021-09-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  6. Article ; Online: Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa.

    Fatoba, Abiodun J / Zishiri, Oliver T / Blake, Damer P / Peters, Sunday O / Lebepe, Jeffrey / Mukaratirwa, Samson / Adeleke, Matthew A

    The Onderstepoort journal of veterinary research

    2020  Volume 87, Issue 1, Page(s) e1–e10

    Abstract: This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were ... ...

    Abstract This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were collected from 12 randomly selected healthy broiler chicken farms and 40 free-range chickens from 10 different locations. Faecal samples were screened for the presence of Eimeria oocysts using a standard flotation method. The species of Eimeria isolates were confirmed by amplification of the internal transcribed spacer 1 (ITS-1) partial region and sequences analysis. Among broiler and free-ranging chickens, 19 out of 41 pens (46.3%) and 25 out of 42 faecal samples (59.5%) were positive for Eimeria infection. Molecular detection revealed the following species: Eimeria maxima, Eimeria tenella, Eimeria acervulina, Eimeria brunetti and Eimeria mitis in all the samples screened. Similarly, polymerase chain reaction assays specific for three cryptic Eimeria operational taxonomic units were negative for all the samples. Phylogenetic analysis of the ITS-1 sequences supported species identity with the greatest variation detected for E. mitis. This study provides information on the range and identity of Eimeria species, and their genetic relatedness, circulating in commercially reared broilers and free-ranging chickens from different locations in KwaZulu-Natal province.
    MeSH term(s) Animals ; Chickens ; Coccidiosis/epidemiology ; Coccidiosis/parasitology ; Coccidiosis/veterinary ; Eimeria/classification ; Eimeria/genetics ; Eimeria/isolation & purification ; Eimeria/physiology ; Feces/parasitology ; Genetic Variation ; Oocysts/isolation & purification ; Phylogeny ; Poultry Diseases/epidemiology ; Poultry Diseases/parasitology ; Prevalence ; South Africa/epidemiology
    Language English
    Publishing date 2020-09-17
    Publishing country South Africa
    Document type Journal Article
    ZDB-ID 417084-2
    ISSN 2219-0635 ; 0030-2465
    ISSN (online) 2219-0635
    ISSN 0030-2465
    DOI 10.4102/ojvr.v87i1.1837
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 893: Show issues Location:
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  7. Article ; Online: Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa

    Abiodun J. Fatoba / Oliver T. Zishiri / Damer P. Blake / Sunday O. Peters / Jeffrey Lebepe / Samson Mukaratirwa / Matthew A. Adeleke

    Onderstepoort Journal of Veterinary Research, Vol 87, Iss 1, Pp e1-e

    2020  Volume 10

    Abstract: This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were ... ...

    Abstract This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were collected from 12 randomly selected healthy broiler chicken farms and 40 free-range chickens from 10 different locations. Faecal samples were screened for the presence of Eimeria oocysts using a standard flotation method. The species of Eimeria isolates were confirmed by amplification of the internal transcribed spacer 1 (ITS-1) partial region and sequences analysis. Among broiler and free-ranging chickens, 19 out of 41 pens (46.3%) and 25 out of 42 faecal samples (59.5%) were positive for Eimeria infection. Molecular detection revealed the following species: Eimeria maxima, Eimeria tenella, Eimeria acervulina, Eimeria brunetti and Eimeria mitis in all the samples screened. Similarly, polymerase chain reaction assays specific for three cryptic Eimeria operational taxonomic units were negative for all the samples. Phylogenetic analysis of the ITS-1 sequences supported species identity with the greatest variation detected for E. mitis. This study provides information on the range and identity of Eimeria species, and their genetic relatedness, circulating in commercially reared broilers and free-ranging chickens from different locations in KwaZulu-Natal province.
    Keywords chickens ; coccidiosis ; eimeria ; genetic diversity ; molecular diagnosis ; prevalence ; Veterinary medicine ; SF600-1100
    Subject code 590
    Language English
    Publishing date 2020-09-01T00:00:00Z
    Publisher AOSIS
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  8. Article: Immunoinformatics approach for multi-epitope vaccine design against P. falciparum malaria

    Maharaj, Leah / Adeleke, Victoria T / Fatoba, Abiodun J / Adeniyi, Adebayo A / Tshilwane, Selaelo I / Adeleke, Matthew A / Maharaj, Rajendra / Okpeku, Moses

    Infection, genetics, and evolution. 2021 Aug., v. 92

    2021  

    Abstract: Plasmodium falciparum (P. falciparum) is a leading causative agent of malaria, an infectious disease that can be fatal. Unfortunately, control measures are becoming less effective over time. A vaccine is needed to effectively control malaria and lead ... ...

    Abstract Plasmodium falciparum (P. falciparum) is a leading causative agent of malaria, an infectious disease that can be fatal. Unfortunately, control measures are becoming less effective over time. A vaccine is needed to effectively control malaria and lead towards the total elimination of the disease. There have been multiple attempts to develop a vaccine, but to date, none have been certified as appropriate for wide-scale use. In this study, an immunoinformatics method is presented to design a multi-epitope vaccine construct predicted to be effective against P. falciparum malaria. This was done through the prediction of 12 CD4+ T-cell, 10 CD8+ T-cell epitopes and, 1 B-cell epitope which were assessed for predicted high antigenicity, immunogenicity, and non-allergenicity through in silico methods. The Human Leukocyte Antigen (HLA) population coverage showed that the alleles associated with the epitopes accounted for 78.48% of the global population. The CD4+ and CD8+ T-cell epitopes were docked to HLA-DRB1*07:01 and HLA-A*32:01 successfully. Therefore, the epitopes were deemed to be suitable as components of a multi-epitope vaccine construct. Adjuvant RS09 was added to the construct to generate a stronger immune response, as confirmed by an immune system simulation. Finally, the structural stability of the predicted multi-epitope vaccine was assessed using molecular dynamics simulations. The results show a promising vaccine design that should be further synthesised and assessed for its efficacy in an experimental laboratory setting.
    Keywords B-lymphocytes ; CD4-positive T-lymphocytes ; CD8-positive T-lymphocytes ; HLA antigens ; Plasmodium falciparum ; adjuvants ; computer simulation ; epitopes ; etiological agents ; evolution ; falciparum malaria ; genetics ; immune response ; immunogenicity ; immunoinformatics ; infection ; infectious diseases ; molecular dynamics ; prediction ; vaccine development ; vaccines
    Language English
    Dates of publication 2021-08
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 2037068-4
    ISSN 1567-1348
    ISSN 1567-1348
    DOI 10.1016/j.meegid.2021.104875
    Database NAL-Catalogue (AGRICOLA)

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