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Article ; Online: Serum-free spontaneously immortalized bovine oviduct epithelial cell conditioned medium promotes the early development of bovine in vitro fertilized embryos.

Miyashita, Norikazu / Akagi, Satoshi / Somfai, Tamas / Hirao, Yuji

The Journal of reproduction and development

2024 Volume 70, Issue 1, Page(s) 42–48

Abstract: Embryonic transfer of bovine blastocysts produced using in vitro fertilization (IVF) is widely used, although the challenge of compromised conception rates remains. Using bovine oviduct epithelial cells (BOEC) to improve embryo culture conditions has ... ...

Abstract	Embryonic transfer of bovine blastocysts produced using in vitro fertilization (IVF) is widely used, although the challenge of compromised conception rates remains. Using bovine oviduct epithelial cells (BOEC) to improve embryo culture conditions has attracted attention, particularly since the recent discovery of extracellular vesicles from BOEC. The selection of embryos for transfer has also been the subject of various studies, and a set of evaluation criteria to predict pregnancy success has been suggested, in which the embryos are judged by their kinetics and morphology at the early stages. In the present study, we established a spontaneously immortalized BOEC line (SI-BOEC) and examined the effects of conditioned medium on IVF embryos, focusing on the results of the recommended criteria. A modified KSOM (mKSOM) was used to prepare conditioned media. Presumptive zygotes were cultured in mKSOM (control), SI-BOEC-conditioned medium, mKSOM supplemented with sediment (pellet) collected after the ultracentrifugation of the conditioned medium (mKSOM/sediment), and the supernatant. A significantly higher percentage of embryos satisfied the recommended criteria when grown in the conditioned medium than in the mKSOM. A higher proportion of embryos developed into blastocysts after achieving the four criteria. A similar tendency was observed when grown in mKSOM/sediment compared to mKSOM; however, this was not observed in the supernatant. Vesicles with a size similar to that of exosomes were observed in the sediment. In conclusion, the culture medium conditioned by SI-BOEC promoted the production of bovine blastocysts that satisfied the four evaluation criteria recommended for embryo selection.
MeSH term(s)	Pregnancy ; Female ; Humans ; Cattle ; Animals ; Culture Media, Conditioned/pharmacology ; Culture Media, Conditioned/metabolism ; Fallopian Tubes ; Oviducts/metabolism ; Embryo, Mammalian ; Epithelial Cells ; Blastocyst ; Fertilization in Vitro/veterinary
Chemical Substances	Culture Media, Conditioned
Language	English
Publishing date	2024-01-19
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2039060-9
ISSN	1348-4400 ; 0916-8818
ISSN (online)	1348-4400
ISSN	0916-8818
DOI	10.1262/jrd.2023-031
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Transit Amplifying Progenitors in the Cerebellum: Similarities to and Differences from Transit Amplifying Cells in Other Brain Regions and between Species.

Miyashita, Satoshi / Hoshino, Mikio

Cells

2022 Volume 11, Issue 4

Abstract: Transit amplification of neural progenitors/precursors is widely used in the development of the central nervous system and for tissue homeostasis. In most cases, stem cells, which are relatively less proliferative, first differentiate into transit ... ...

Abstract	Transit amplification of neural progenitors/precursors is widely used in the development of the central nervous system and for tissue homeostasis. In most cases, stem cells, which are relatively less proliferative, first differentiate into transit amplifying cells, which are more proliferative, losing their stemness. Subsequently, transit amplifying cells undergo a limited number of mitoses and differentiation to expand the progeny of differentiated cells. This step-by-step proliferation is considered an efficient system for increasing the number of differentiated cells while maintaining the stem cells. Recently, we reported that cerebellar granule cell progenitors also undergo transit amplification in mice. In this review, we summarize our and others' recent findings and the prospective contribution of transit amplification to neural development and evolution, as well as the molecular mechanisms regulating transit amplification.
MeSH term(s)	Animals ; Brain ; Cell Differentiation/physiology ; Cerebellum ; Mice ; Neurogenesis/physiology ; Prospective Studies
Language	English
Publishing date	2022-02-18
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2661518-6
ISSN	2073-4409 ; 2073-4409
ISSN (online)	2073-4409
ISSN	2073-4409
DOI	10.3390/cells11040726
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Article ; Online: Transit Amplifying Progenitors in the Cerebellum

Satoshi Miyashita / Mikio Hoshino

Cells, Vol 11, Iss 726, p

Similarities to and Differences from Transit Amplifying Cells in Other Brain Regions and between Species

2022 Volume 726

Abstract	Transit amplification of neural progenitors/precursors is widely used in the development of the central nervous system and for tissue homeostasis. In most cases, stem cells, which are relatively less proliferative, first differentiate into transit amplifying cells, which are more proliferative, losing their stemness. Subsequently, transit amplifying cells undergo a limited number of mitoses and differentiation to expand the progeny of differentiated cells. This step-by-step proliferation is considered an efficient system for increasing the number of differentiated cells while maintaining the stem cells. Recently, we reported that cerebellar granule cell progenitors also undergo transit amplification in mice. In this review, we summarize our and others’ recent findings and the prospective contribution of transit amplification to neural development and evolution, as well as the molecular mechanisms regulating transit amplification.
Keywords	neural development ; cerebellar granule cell prognitors ; transit amplification ; ATOH1 ; NEUROD1 ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [The role of advance care planning and palliative care in new york city during COVID-19 pandemic].

Fujisaki, Tomohiro / Miyashita, Satoshi

Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics

2021 Volume 58, Issue 1, Page(s) 41–46

Abstract: New York City faced an explosive spread of Coronavirus disease 2019 (COVID-19), causing the city's death toll to spike owing to the high virulence of COVID-19. The health care system was on the verge of collapse. Advance care planning (ACP), informed ... ...

Abstract	New York City faced an explosive spread of Coronavirus disease 2019 (COVID-19), causing the city's death toll to spike owing to the high virulence of COVID-19. The health care system was on the verge of collapse. Advance care planning (ACP), informed assent, and palliative care played significant roles in supporting patient self-determination and dignity, facilitating decision making, and promoting better care. The importance of these strategies was revisited. Learning from the COVID-19 pandemic in New York City, it is anticipated that several approaches such as ACP and palliative intervention may attract more attention and become increasingly essential to the healthcare system in Japan.
MeSH term(s)	Advance Care Planning ; COVID-19 ; Humans ; Japan ; New York City/epidemiology ; Palliative Care ; Pandemics ; SARS-CoV-2
Language	Japanese
Publishing date	2021-02-24
Publishing country	Japan
Document type	Journal Article
ZDB-ID	604107-3
ISSN	0300-9173
ISSN	0300-9173
DOI	10.3143/geriatrics.58.41
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Article ; Online: Glycogen synthase kinase 3β: the nexus of chemoresistance, invasive capacity, and cancer stemness in pancreatic cancer.

Uehara, Masahiro / Domoto, Takahiro / Takenaka, Satoshi / Takeuchi, Osamu / Shimasaki, Takeo / Miyashita, Tomoharu / Minamoto, Toshinari

Cancer drug resistance (Alhambra, Calif.)

2024 Volume 7, Page(s) 4

Abstract: The treatment of pancreatic cancer remains a significant clinical challenge due to the limited number of patients eligible for curative (R0) surgery, failures in the clinical development of targeted and immune therapies, and the pervasive acquisition of ... ...

Abstract	The treatment of pancreatic cancer remains a significant clinical challenge due to the limited number of patients eligible for curative (R0) surgery, failures in the clinical development of targeted and immune therapies, and the pervasive acquisition of chemotherapeutic resistance. Refractory pancreatic cancer is typified by high invasiveness and resistance to therapy, with both attributes related to tumor cell stemness. These malignant characteristics mutually enhance each other, leading to rapid cancer progression. Over the past two decades, numerous studies have produced evidence of the pivotal role of glycogen synthase kinase (GSK)3β in the progression of over 25 different cancer types, including pancreatic cancer. In this review, we synthesize the current knowledge on the pathological roles of aberrant GSK3β in supporting tumor cell proliferation and invasion, as well as its contribution to gemcitabine resistance in pancreatic cancer. Importantly, we discuss the central role of GSK3β as a molecular hub that mechanistically connects chemoresistance, tumor cell invasion, and stemness in pancreatic cancer. We also discuss the involvement of GSK3β in the formation of desmoplastic tumor stroma and in promoting anti-cancer immune evasion, both of which constitute major obstacles to successful cancer treatment. Overall, GSK3β has characteristics of a promising therapeutic target to overcome chemoresistance in pancreatic cancer.
Language	English
Publishing date	2024-01-31
Publishing country	United States
Document type	Journal Article ; Review
ISSN	2578-532X
ISSN (online)	2578-532X
DOI	10.20517/cdr.2023.84
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Article ; Online: Deciphering the role of adjuvant therapy in melanoma and its actual benefits.

Fukushima, Satoshi / Miyashita, Azusa / Kimura, Toshihiro / Kuriyama, Haruka / Mizuhashi, Satoru / Ichigozaki, Yuki / Masuguchi, Shinichi

The Journal of dermatology

2024 Volume 51, Issue 3, Page(s) 335–342

Abstract: Numerous clinical trials have demonstrated a significant improvement in recurrence-free survival among melanoma patients receiving high-dose interferon-α, immune checkpoint inhibitors (pembrolizumab, nivolumab), and BRAF/MEK inhibitors (dabrafenib- ... ...

Abstract	Numerous clinical trials have demonstrated a significant improvement in recurrence-free survival among melanoma patients receiving high-dose interferon-α, immune checkpoint inhibitors (pembrolizumab, nivolumab), and BRAF/MEK inhibitors (dabrafenib-trametinib). This study aimed to investigate whether these findings hold true in real-world conditions for patients with stage III and IV melanoma. In particular, the study explores the efficacy and side effects of adjuvant therapies, focusing on anti-PD-1 antibodies and BRAF/MEK inhibitors. While clinical trials have shown comparable efficacy, differences in side-effect profiles, especially the persistence of immune-related adverse events with anti-PD-1 antibodies, highlight the need for careful consideration in adjuvant settings. In the absence of established biomarkers for guiding adjuvant therapy decisions, it becomes imperative to transparently communicate the advantages and disadvantages of drug administration to patients. The study also delved into the impact of melanoma subtype and BRAF mutation status on the effectiveness of adjuvant therapy, emphasizing the need for further investigation.
MeSH term(s)	Humans ; Melanoma/drug therapy ; Melanoma/genetics ; Skin Neoplasms/drug therapy ; Skin Neoplasms/genetics ; Proto-Oncogene Proteins B-raf/genetics ; Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Imidazoles/adverse effects ; Mitogen-Activated Protein Kinase Kinases
Chemical Substances	Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; Imidazoles ; Mitogen-Activated Protein Kinase Kinases (EC 2.7.12.2)
Language	English
Publishing date	2024-01-11
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	800103-0
ISSN	1346-8138 ; 0385-2407
ISSN (online)	1346-8138
ISSN	0385-2407
DOI	10.1111/1346-8138.17093
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Article ; Online: Draft Genome Sequence of the Cyanobacterium

Pierpont, Christopher L / Ohkubo, Satoshi / Miyashita, Hideaki / Miller, Scott R

Microbiology resource announcements

2022 Volume 11, Issue 5, Page(s) e0025122

Abstract: We report the 3.5-Mb draft genome sequence of the ... ...

Abstract	We report the 3.5-Mb draft genome sequence of the cyanobacterium
Language	English
Publishing date	2022-04-19
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/mra.00251-22
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Article ; Online: Preventive effects of skin protectants on the development of medical device-related pressure wounds in endoscopic sinonasal surgery.

Miyashita, Keisuke / Tochigi, Kousuke / Omura, Kazuhiro / Aoki, Satoshi / Tanaka, Yasuhiro

Auris, nasus, larynx

2023 Volume 50, Issue 6, Page(s) 880–886

Abstract: Objectives: Medical device-related pressure ulcer (MDRPU) is a skin or subcutaneous tissue injury caused by medical devices. Skin protectants have been used to prevent MDRPU in other fields. In endoscopic sinonasal surgery (ESNS), rigid endoscopes and ... ...

Abstract	Objectives: Medical device-related pressure ulcer (MDRPU) is a skin or subcutaneous tissue injury caused by medical devices. Skin protectants have been used to prevent MDRPU in other fields. In endoscopic sinonasal surgery (ESNS), rigid endoscopes and forceps can cause MDRPU; however, detailed investigations have not been conducted. This study aimed to investigate the frequency of MDRPU in ESNS and the preventive effects of skin protectants METHODS: Thirty-nine patients who received ESNS and consented to study participation were randomly assigned to the "protective agent" (n = 18) or "control" (n = 21) group. MDRPU presence around the nostril was evaluated for up to 7 days post-surgically based on physical findings and subjective symptoms. The occurrence ratio and severity of MDRPU were statistically compared between the groups to evaluate the efficacy of skin protective agents. Results: Stage 1 MDRPU, according to the National Pressure Ulcer Advisory Panel classification, was seen in 20.5% (8/39) of the patients, and no patient had more high-grade ulceration. On postoperative days 2 and 3, skin erythema was predominantly observed on the nasal floor, with a comparatively lower incidence in the protective agent group. Significant pain reduction was observed in the nostril's floor on postoperative days 2 and 3 in the protective agent group. Conclusions: MDRPU occurred with a relatively high frequency around the nostrils after ESNS. Protective agent use in the external nostrils was effective especially in reducing post-operative pain on the nasal floor, where tissue damage can easily occur due to device-related friction.
MeSH term(s)	Humans ; Pressure Ulcer/prevention & control ; Pressure Ulcer/epidemiology ; Pressure Ulcer/etiology ; Endoscopy/adverse effects ; Nasal Cavity ; Endoscopes/adverse effects ; Surgical Instruments/adverse effects
Language	English
Publishing date	2023-03-09
Publishing country	Netherlands
Document type	Randomized Controlled Trial ; Journal Article
ZDB-ID	604552-2
ISSN	1879-1476 ; 0385-8146
ISSN (online)	1879-1476
ISSN	0385-8146
DOI	10.1016/j.anl.2023.02.005
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Zs.A 1675: Show issues

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Article ; Online: A rare case of echo enhancing agent anaphylaxis complicated by acute coronary syndrome.

Steinhauer, Rachael / Miyashita, Satoshi / Chweich, Haval / Ortoleva, Jamel / Downey, Brian C

Echocardiography (Mount Kisco, N.Y.)

2023 Volume 40, Issue 7, Page(s) 743–746

Abstract: Enhancing agents in echocardiography are used frequently to improve endocardial border visualization and assessment of structural heart disease. We present a unique case of anaphylactic shock with acute coronary syndrome following administration of ... ...

Abstract	Enhancing agents in echocardiography are used frequently to improve endocardial border visualization and assessment of structural heart disease. We present a unique case of anaphylactic shock with acute coronary syndrome following administration of sulfur hexafluoride echo enhancing agent. This case emphasizes the importance of recognizing anaphylaxis to enhancing agents, as well as recognizing the potential relationship between anaphylaxis and acute coronary syndrome with in-stent thrombosis.
MeSH term(s)	Humans ; Anaphylaxis/chemically induced ; Anaphylaxis/complications ; Anaphylaxis/diagnosis ; Acute Coronary Syndrome/complications ; Acute Coronary Syndrome/diagnostic imaging ; Contrast Media/adverse effects ; Sulfur Hexafluoride ; Echocardiography
Chemical Substances	Contrast Media ; Sulfur Hexafluoride (WS7LR3I1D6)
Language	English
Publishing date	2023-05-19
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	843645-9
ISSN	1540-8175 ; 0742-2822
ISSN (online)	1540-8175
ISSN	0742-2822
DOI	10.1111/echo.15596
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Zs.A 2170: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: Metastasis to hypopharynx from epidermotropic metastatic malignant melanoma.

Mizuhashi, Satoru / Miyashita, Azusa / Kuriyama, Haruka / Kimura, Toshihiro / Kanemaru, Hisashi / Miyamaru, Satoru / Saeki, Sho / Fukushima, Satoshi

Drug discoveries & therapeutics

2024

Abstract: Previous reports proposed the concept and criteria of epidermotropic metastatic malignant melanoma (EMMM): (a) dermal involvement equal to or broader than the epidermal involvement, (b) atypical melanocytes within the dermis, (c) thinning of the ... ...

Abstract	Previous reports proposed the concept and criteria of epidermotropic metastatic malignant melanoma (EMMM): (a) dermal involvement equal to or broader than the epidermal involvement, (b) atypical melanocytes within the dermis, (c) thinning of the epidermis, (d) widening of the papillary dermis with an epithelial collarette, and (e) vascular invasion of atypical melanocytes. However, it remains unclear whether EMMM also involves the mucosal epithelium. In this case, the patient was diagnosed with EMMM based on the histopathological findings of the patient's multiple skin lesions and clinical course. The patient also developed metastasis to the hypopharynx. Although histopathological findings of the lesion suggested the possibility of melanoma in situ, as the lesion included atypical melanocytes in the mucosal epithelium, the clinical course supported the diagnosis of hypopharyngeal metastasis from EMMM. This case suggests that EMMM may have epitheliotropic features not only in the skin but also in the mucosa.
Language	English
Publishing date	2024-04-17
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2568828-5
ISSN	1881-784X ; 1881-784X
ISSN (online)	1881-784X
ISSN	1881-784X
DOI	10.5582/ddt.2024.01017
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