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  1. Book: Site-Specific recombinases

    Eroshenko, Nikolai

    methods and protocols

    (Methods in molecular biology ; 1642 ; Springer protocols)

    2017  

    Author's details edited by Nikolai Eroshenko
    Series title Methods in molecular biology ; 1642
    Springer protocols
    Collection
    Keywords CRISPR ; protein design ; recombinase delivery ; recombinase specificity alteration ; synthetic biology
    Subject code 610
    Language English
    Size xi, 342 Seiten, Illustrationen, 25.4 cm x 17.8 cm
    Publisher Humana Press
    Publishing place New York, NY
    Publishing country United States
    Document type Book
    HBZ-ID HT019375842
    ISBN 978-1-4939-7167-1 ; 1-4939-7167-0 ; 9781493971695 ; 1493971697
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    Zs A 7042 -1642- verfügbar in Königswinter Königswinter

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  2. Book ; Online: Site-specific recombinases

    Eroshenko, Nikolai

    methods and protocols

    2017  

    Keywords Text ; ddc:610
    Language English
    Publisher Humana Press
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Book: Site-specific recombinases

    Eroshenko, Nikolai

    methods and protocols

    (Methods in molecular biology ; 1642 ; Springer protocols)

    2017  

    Abstract: This book aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in ... ...

    Author's details edited by Nikolai Eroshenko
    Series title Methods in molecular biology ; 1642
    Springer protocols
    Abstract This book aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.
    Keywords Genetic recombination ; Recombinant DNA ; genetic recombination ; genetics ; site-directed mutagenesis ; Genetic recombination. ; Recombinant DNA.
    Language English
    Dates of publication 2017-2017
    Size xi, 342 pages :, illustrations (some color) ;, 27 cm.
    Document type Book
    ISBN 1493971670 ; 9781493971671 ; 9781493971695 ; 1493971697
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Retrospective analysis of adaptive properties of barley varieties bred by FRC «Nemchinovka»

    Lyubov M. Eroshenko / Maksim M. Romakhin / Anatoly N. Eroshenko / Nikolai A. Eroshenko / Ivan A. Dedushev / Victoria V. Romakhina / Mikhail A. Boldyrev

    Agrarnaâ nauka Evro-Severo-Vostoka. 2021 Aug., v. 22, no. 4

    2021  

    Abstract: The article presents the results of six-year studies (2015-2020) of 19 varieties of spring barley belonging to different periods of variety changing bred by FRC “Nemchinovka". The research was aimed at comprehensive assessment of yield, adaptive features ...

    Abstract The article presents the results of six-year studies (2015-2020) of 19 varieties of spring barley belonging to different periods of variety changing bred by FRC “Nemchinovka". The research was aimed at comprehensive assessment of yield, adaptive features and other agronomic characters and properties of spring barley for determining the main tendencies of changing their properties during many years of breeding work in the conditions of the Central Non-Chernozem Zone. Due to high potential productivity, which was demonstrated in optimal conditions for barley and the ability of the crop to resist yield reduction during bad weather conditions, the high-intense barley varieties zoned during last 20 years, proved to be the best according to average yield indicators. Maximum average yield in these groups was 8.17 t/h, minimum one was 3.05 t/h that was higher than average yield among varieties in previous periods of breeding work by 19.3 % and 10.5 %, respectively. The retrospective analysis determined the direction of breeding shifts in adaptive properties and productivity elements associated with an increase in the economic and biological value of barley varieties in the process of selective breeding improvement. Judging by the average plasticity indices (V = 35.4 %; bi = 1.13; σ = 2.12) the varieties of the latest stages of breeding work were 18.8; 36.1 and 37.7 % more responsive to improving environmental conditions as compared to the varieties of the last century. The relative average value of the adaptability coefficient exceeding unity (KA = 1.02-1.19) indicated a stronger reaction of modern barley varieties to resist the action of factors that reduce their potential productivity. With yield growth and increase in adaptability, the architectonics of barley plants was changing and resistance to lodging and helminthosporiosis raised. There has been established a tendency to increase the most important element of the crop structure "the number of productive ears per 1 m2" and to decrease the indicator "weight of 1000 grains". The information obtained on the basis of a comprehensive assessment of varieties by economic and biological indicators makes it possible to determine the ways of selective breeding improvement of the crop.
    Keywords plasticity ; retrospective studies ; spring barley ; weather
    Language English
    Dates of publication 2021-08
    Size p. 485-494.
    Publishing place Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
    Document type Article
    ISSN 2500-1396
    DOI 10.30766/2072-9081.2021.22.4.485-494
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Implications of antibody-dependent enhancement of infection for SARS-CoV-2 countermeasures.

    Eroshenko, Nikolai / Gill, Taylor / Keaveney, Marianna K / Church, George M / Trevejo, Jose M / Rajaniemi, Hannu

    Nature biotechnology

    2020  Volume 38, Issue 7, Page(s) 789–791

    MeSH term(s) Animals ; Antibody-Dependent Enhancement ; Betacoronavirus/physiology ; COVID-19 ; COVID-19 Vaccines ; Coronavirus Infections/immunology ; Coronavirus Infections/prevention & control ; Coronavirus Infections/virology ; Disease Models, Animal ; Humans ; Pandemics ; Pneumonia, Viral/immunology ; Pneumonia, Viral/virology ; Primates ; SARS Virus/physiology ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/metabolism ; Viral Vaccines/immunology ; Virus Diseases/immunology ; Virus Diseases/virology
    Chemical Substances COVID-19 Vaccines ; Spike Glycoprotein, Coronavirus ; Viral Vaccines
    Keywords covid19
    Language English
    Publishing date 2020-05-20
    Publishing country United States
    Document type Letter
    ZDB-ID 1311932-1
    ISSN 1546-1696 ; 1087-0156
    ISSN (online) 1546-1696
    ISSN 1087-0156
    DOI 10.1038/s41587-020-0577-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2167: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 137: Show issues
    Zs.MG 43: Show issues

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Retrospective analysis of adaptive properties of barley varieties bred by FRC «Nemchinovka»

    Lyubov M. Eroshenko / Maksim M. Romakhin / Anatoly N. Eroshenko / Nikolai A. Eroshenko / Ivan A. Dedushev / Victoria V. Romakhina / Mikhail A. Boldyrev

    Аграрная наука Евро-Северо-Востока, Vol 22, Iss 4, Pp 485-

    2021  Volume 494

    Abstract: The article presents the results of six-year studies (2015-2020) of 19 varieties of spring barley belonging to different periods of variety changing bred by FRC “Nemchinovka". The research was aimed at comprehensive assessment of yield, adaptive features ...

    Abstract The article presents the results of six-year studies (2015-2020) of 19 varieties of spring barley belonging to different periods of variety changing bred by FRC “Nemchinovka". The research was aimed at comprehensive assessment of yield, adaptive features and other agronomic characters and properties of spring barley for determining the main tendencies of changing their properties during many years of breeding work in the conditions of the Central Non-Chernozem Zone. Due to high potential productivity, which was demonstrated in optimal conditions for barley and the ability of the crop to resist yield reduction during bad weather conditions, the high-intense barley varieties zoned during last 20 years, proved to be the best according to average yield indicators. Maximum average yield in these groups was 8.17 t/h, minimum one was 3.05 t/h that was higher than average yield among varieties in previous periods of breeding work by 19.3 % and 10.5 %, respectively. The retrospective analysis determined the direction of breeding shifts in adaptive properties and productivity elements associated with an increase in the economic and biological value of barley varieties in the process of selective breeding improvement. Judging by the average plasticity indices (V = 35.4 %; bi = 1.13; σ = 2.12) the varieties of the latest stages of breeding work were 18.8; 36.1 and 37.7 % more responsive to improving environmental conditions as compared to the varieties of the last century. The relative average value of the adaptability coefficient exceeding unity (KA = 1.02-1.19) indicated a stronger reaction of modern barley varieties to resist the action of factors that reduce their potential productivity. With yield growth and increase in adaptability, the architectonics of barley plants was changing and resistance to lodging and helminthosporiosis raised. There has been established a tendency to increase the most important element of the crop structure "the number of productive ears per 1 m2" and to decrease the indicator "weight of ...
    Keywords spring barley ; variety ; yield ; adaptability ; resistance ; variety changing ; Agriculture ; S
    Subject code 630
    Language Russian
    Publishing date 2021-08-01T00:00:00Z
    Publisher Federal Agricultural Research Center of the North-East named N.V. Rudnitsky
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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  7. Article: Implications of antibody-dependent enhancement of infection for SARS-CoV-2 countermeasures

    Eroshenko, Nikolai / Gill, Taylor / Keaveney, Marianna K / Church, George M / Trevejo, Jose M / Rajaniemi, Hannu

    Nat. biotechnol

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #32504046
    Database COVID19

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  8. Article ; Online: Mutants of Cre recombinase with improved accuracy.

    Eroshenko, Nikolai / Church, George M

    Nature communications

    2013  Volume 4, Page(s) 2509

    Abstract: Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery ... ...

    Abstract Despite rapid advances in genome engineering technologies, inserting genes into precise locations in the human genome remains an outstanding problem. It has been suggested that site-specific recombinases can be adapted towards use as transgene delivery vectors. The specificity of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding domains. Unfortunately, both wild-type and altered variants often have detectable activities at off-target sites. Here we use bacterial selections to identify mutations in the dimerization surface of Cre recombinase (R32V, R32M and 303GVSdup) that improve the accuracy of recombination. The mutants are functional in bacteria, in human cells and in vitro (except for 303GVSdup, which we did not purify), and have improved selectivity against both model off-target sites and the entire E. coli genome. We propose that destabilizing binding cooperativity may be a general strategy for improving the accuracy of dimeric DNA-binding proteins.
    MeSH term(s) Base Sequence ; Cell Line ; DNA/chemistry ; DNA/metabolism ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Transfer Techniques ; Humans ; Integrases/genetics ; Integrases/metabolism ; Kinetics ; Models, Molecular ; Molecular Sequence Data ; Mutation ; Protein Multimerization ; Recombination, Genetic ; Transgenes ; Viral Proteins/genetics ; Viral Proteins/metabolism
    Chemical Substances DNA-Binding Proteins ; Viral Proteins ; DNA (9007-49-2) ; Cre recombinase (EC 2.7.7.-) ; Integrases (EC 2.7.7.-)
    Language English
    Publishing date 2013-09-05
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/ncomms3509
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Implications of antibody-dependent enhancement of infection for SARS-CoV-2 countermeasures

    Eroshenko, Nikolai / Gill, Taylor / Keaveney, Marianna K. / Church, George M. / Trevejo, Jose M. / Rajaniemi, Hannu

    Nature Biotechnology

    2020  Volume 38, Issue 7, Page(s) 789–791

    Keywords Biotechnology ; Molecular Medicine ; Applied Microbiology and Biotechnology ; Bioengineering ; Biomedical Engineering ; covid19
    Language English
    Publisher Springer Science and Business Media LLC
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1311932-1
    ISSN 1546-1696 ; 1087-0156
    ISSN (online) 1546-1696
    ISSN 1087-0156
    DOI 10.1038/s41587-020-0577-1
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2167: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 137: Show issues
    Zs.MG 43: Show issues

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  10. Article ; Online: Gene Assembly from Chip-Synthesized Oligonucleotides.

    Eroshenko, Nikolai / Kosuri, Sriram / Marblestone, Adam H / Conway, Nicholas / Church, George M

    Current protocols in chemical biology

    2014  Volume 2012

    Abstract: ... De ... ...

    Abstract De novo
    Language English
    Publishing date 2014-06-06
    Publishing country United States
    Document type Journal Article
    ISSN 2160-4762
    ISSN (online) 2160-4762
    DOI 10.1002/9780470559277.ch110190
    Database MEDical Literature Analysis and Retrieval System OnLINE

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