Article ; Online: Assessment of the anti-rheumatoid arthritis activity of Gastrodia elata (tian-ma) and Radix aconitic lateralis preparata (fu-zi) via network pharmacology and untargeted metabolomics analyses.
International journal of rheumatic diseases
2021 Volume 24, Issue 3, Page(s) 380–390
Abstract: ... with our network pharmacological predictions.: Conclusions: The anti-RA properties of Tian-Ma and Fu-Zi are ... Aim: Gastrodia elata and Radix aconiti lateralis preparrata are respectively named as Tian-Ma and ... Fu-Zi (TF) in Chinese. We explored the active components against rheumatoid arthritis (RA ...
| Abstract | Aim: Gastrodia elata and Radix aconiti lateralis preparrata are respectively named as Tian-Ma and Fu-Zi (TF) in Chinese. We explored the active components against rheumatoid arthritis (RA) from an extensively used couplet of Chinese herbs, Gastrodia elata and Radix aconiti lateralis preparata (TF) via untargeted metabolomics and network pharmacological approaches. Methods: Water extracts of TF were mixed at ratios 1:1, 3:2 and 2:3 (w/w). Ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) was then utilized as metabolomics screening. Human Metabolome (http://www.hmdb.ca/) and Lipidmaps (http://www.lipidmaps.org/) databases were used to annotate detected compounds. Further identification of vital genes and important pathways associated with the anti-RA properties of the TF preparations was done via network pharmacology, and verified by real-time quantitative polymerase chain reaction (RT-qPCR). Results: Four key compounds involved in unsaturated fatty acid biosynthesis and isoflavonoid biosynthesis were identified through metabolomics analyses. Three key components of TF associated with anti-RA activity were linoleic acid, daidzein, and daidzin. Results of RT-qPCR revealed that all 3 tested TF couplets (1:1, 3:2, and 2:3) markedly suppressed the transcription of PTGS2. These results were consistent with our network pharmacological predictions. Conclusions: The anti-RA properties of Tian-Ma and Fu-Zi are associated with the inhibition of arachidonic acid metabolism pathway. |
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| MeSH term(s) | Aconitum ; Animals ; Arachidonic Acid/antagonists & inhibitors ; Arachidonic Acid/metabolism ; Arthritis, Rheumatoid/drug therapy ; Arthritis, Rheumatoid/genetics ; Arthritis, Rheumatoid/metabolism ; Chromatography, Liquid ; Cyclooxygenase 1/biosynthesis ; Cyclooxygenase 1/genetics ; Cyclooxygenase 2/biosynthesis ; Cyclooxygenase 2/genetics ; DNA/genetics ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Gastrodia ; Gene Expression Regulation/drug effects ; Humans ; Male ; Membrane Proteins/biosynthesis ; Membrane Proteins/genetics ; Metabolomics/methods ; Plant Extracts/pharmacology ; Rats ; Rats, Sprague-Dawley ; Tandem Mass Spectrometry |
| Chemical Substances | Drugs, Chinese Herbal ; Membrane Proteins ; Plant Extracts ; Arachidonic Acid (27YG812J1I) ; DNA (9007-49-2) ; Cyclooxygenase 1 (EC 1.14.99.1) ; Cyclooxygenase 2 (EC 1.14.99.1) ; Ptgs1 protein, rat (EC 1.14.99.1) ; Ptgs2 protein, rat (EC 1.14.99.1) |
| Language | English |
| Publishing date | 2021-02-01 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2426924-4 |
| ISSN | 1756-185X ; 1756-1841 |
| ISSN (online) | 1756-185X |
| ISSN | 1756-1841 |
| DOI | 10.1111/1756-185X.14063 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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