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Article ; Online: Detection of Cistanches Herba (Rou Cong Rong) Medicinal Products Using Species-Specific Nucleotide Signatures

Xiao-yue Wang / Rong Xu / Jun Chen / Jing-yuan Song / Steven-G Newmaster / Jian-ping Han / Zheng Zhang / Shi-lin Chen

Frontiers in Plant Science, Vol

2018 Volume 9

Abstract: Cistanches Herba is a medicinal plant that has tonification properties and is commonly used in Asia. Owing to the imbalance between supply and demand, adulterants are frequently added for profit. However, there is no regulatory oversight because quality ... ...

Abstract	Cistanches Herba is a medicinal plant that has tonification properties and is commonly used in Asia. Owing to the imbalance between supply and demand, adulterants are frequently added for profit. However, there is no regulatory oversight because quality control tools are not sufficient for identifying heavily processed products. Thus, a novel molecular tool based on nucleotide signatures and species-specific primers was developed. The ITS2 regions from 251 Cistanches Herba and adulterant samples were sequenced. On the basis of SNP sites, four nucleotide signatures within 30~37 bp and six species-specific primers were developed, and they were validated by artificial experimental mixtures consisting of six different species and different ratios. This method was also applied to detect 66 Cistanches Herba products on the market, including extracts and Chinese patent medicines. The results demonstrated the utility of nucleotide signatures in identifying adulterants in mixtures. The market study revealed 36.4% adulteration: 19.7% involved adulteration with Cynomorium songaricum or Cistanche sinensis, and 16.7% involved substitution with Cy. songaricum, Ci. sinensis, or Boschniakia rossica. The results also revealed that Cy. songaricum was the most common adulterant in the market. Thus, we recommend the use of species-specific nucleotide signatures for regulating adulteration and verifying the quality assurance of medicinal product supply chains, especially for processed products whose DNA is degraded.
Keywords	Cistanches Herba ; Chinese patent medicine ; nucleotide signature ; degraded DNA ; medicine quality control ; Plant culture ; SB1-1110
Subject code	590
Language	English
Publishing date	2018-11-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Answer to the Letter to the Editor of A. Gardner concerning "Comparison of combined anterior-posterior approach versus posterior-only approach in treating adolescent idiopathic scoliosis: a meta-analysis" by Chen Z, Rong L (2016) Eur Spine J;25(2):363-371.

Chen, Zihao / Rong, Limin

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society

2016 Volume 25, Issue 11, Page(s) 3766–3767

Language	English
Publishing date	2016-11
Publishing country	Germany
Document type	Letter
ZDB-ID	1115375-1
ISSN	1432-0932 ; 0940-6719
ISSN (online)	1432-0932
ISSN	0940-6719
DOI	10.1007/s00586-016-4734-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 3437: Show issues

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Article ; Online: Clinical efficacy of Rong-Yang-Jyh-Gan-Tang on patients with chronic hepatitis C: A double-blinded randomized placebo-controlled crossover study.

Chen, Fang-Pey / Chang, Ching-Mao / Wu, Ta-Peng / Yang, Jen-Lin / Kung, Yen-Ying / Huang, Yi-Hsiang / Su, Chien-Wei / Lan, Keng-Hsin / Chiang, Shu-Chiung / Hwang, Shinn-Jang

Journal of ethnopharmacology

2017 Volume 196, Page(s) 1–8

Abstract: ... such as serum viral load to evaluate treatment response.: Aim of the study: We evaluated the efficacy of Rong ...

Abstract	Ethnopharmacological relevance: Traditional Chinese medicine (TCM) has been widely used by the Chinese population for treatment of chronic hepatitis. However, the efficacy of TCM for patients with chronic hepatitis has not been confirmed, mostly due to the lack of available scientific parameters such as serum viral load to evaluate treatment response. Aim of the study: We evaluated the efficacy of Rong-Yang-Jyh-Gan-Tang (RYJGT, composed of Long-Dan-Xie-Gan-Tang, Jia-Wei-Xia-Yao-San, Dan-Shen, and Hou-Po) on patients with chronic hepatitis C. Materials and methods: Thirty-six patients with chronic hepatitis C who had no response to or had contraindications to interferon-ribavirin therapy were randomly allocated to receive RYJGT 15g/day or placebo for 12 weeks. After a 2-week washout period, patients were crossed over to receive placebo or RYJGT for another 12 weeks. Evaluation parameters included liver biochemistries, serum HCVRNA, side effects of RYJGT/placebo, and TCM symptoms. Results: Of the patients who had 12-week RYJGT treatment, 51.7% had decreased serum HCVRNA levels, whereas only 25.8% patients had decreased levels in the placebo group (p=0.036). TCM patterns of "Damp-Heat" and "Liver Qi Depression" had significantly improved after RYJGT treatment in comparison with the placebo. Logistic analyses showed that RYJGT treatment, and pre-treatment values of TCM symptoms of "Damp-Heat" and "Liver Qi Depression", were statistically significant factors in predicting the decrease in serum HCVRNA. Conclusion: Chronic hepatitis C patients who received a 12-week RYJGT treatment had significantly higher HCVRNA decrease ratio, and improved TCM symptoms of "Damp-Heat" and "Liver Qi Depression", than those who received the placebo. Our results require further larger scale clinical trials.
MeSH term(s)	Aged ; Aged, 80 and over ; Alanine Transaminase/blood ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Aspartate Aminotransferases/blood ; Cross-Over Studies ; Double-Blind Method ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Female ; Hepacivirus/genetics ; Hepatitis C, Chronic/blood ; Hepatitis C, Chronic/drug therapy ; Hepatitis C, Chronic/virology ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; RNA, Viral/blood ; Treatment Outcome
Chemical Substances	Antiviral Agents ; Drugs, Chinese Herbal ; RNA, Viral ; rong-yang-jyh-gan-tang ; Aspartate Aminotransferases (EC 2.6.1.1) ; Alanine Transaminase (EC 2.6.1.2)
Language	English
Publishing date	2017-01-20
Publishing country	Ireland
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2016.12.013
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Zs.A 1494: Show issues

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Article ; Online: Use of traditional Chinese medicine (Ren Shen Yang Rong Tang) against microinflammation in hemodialysis patients: An open-label trial.

Hsiao, Po-Jen / Lin, Kao-Shin / Chiu, Chih-Chien / Chen, Hsueh-Wen / Huang, Jing-Shu / Kao, Sen-Yeong / Lin, Yuh-Feng / Chen, Jin-Shuen

Complementary therapies in medicine

2015 Volume 23, Issue 3, Page(s) 363–371

Abstract: ... of Ren Shen Yang Rong Tang (R-S-Y-R-T) in hemodialysis (HD) patients.: Methods: The experimental group ...

Abstract	Background: Complementary and alternative medicine such as traditional Chinese medicine (TCM) is now frequently used combined with Western medicine for treatment in chronic kidney disease (CKD). Objective: We designed an open-label trial to investigate the safety and potential therapeutic effects of Ren Shen Yang Rong Tang (R-S-Y-R-T) in hemodialysis (HD) patients. Methods: The experimental group was treated with additional R-S-Y-R-T combined with routine western medicine, while the control group was treated only with routine western medicine. The duration of study was 6 months. Primary outcomes were to evaluate the changes in serum hematocrit and albumin levels. Secondary outcomes including blood inflammatory markers (c-reactive protein [CRP], interleukin-6 [IL-6], and tumor necrosis factor-α [TNF-α]) were checked. Finally we also followed up the change of quality of life (QOL) in our subjects. Results: Sixty nine respondents were enrolled in this trial. Finally a total of 59 patients (27 R-S-Y-R-T group, 32 control group) completed the 6-month follow-up. Primary outcomes showed no significant statistical change of hematocrit in either 2 group (P>0.05). But the R-S-Y-R-T group had a statistical increase in serum albumin (P<0.05). Secondary outcomes were that both TNF-α (P=0.003) and IL-6 (P=0.001) showed evident decrease in the R-S-Y-R-T group. CRP was identified without statistical difference in both groups (P=0.226). The R-S-Y-R-T group also had a significant improvement in QOL (P<0.05). Conclusions: Our study suggests that R-S-Y-R-T could decrease chronic inflammation and increase the life quality in HD patients. Further larger clinical trial of long-term treatment with R-S-Y-R-T is necessary for evaluating treatment use.
MeSH term(s)	Aged ; Blood Pressure ; C-Reactive Protein/analysis ; Drugs, Chinese Herbal/adverse effects ; Drugs, Chinese Herbal/therapeutic use ; Female ; Humans ; Inflammation/epidemiology ; Inflammation/therapy ; Interleukin-6/blood ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Prospective Studies ; Quality of Life ; Renal Dialysis/adverse effects ; Tumor Necrosis Factor-alpha/blood
Chemical Substances	Drugs, Chinese Herbal ; Interleukin-6 ; Tumor Necrosis Factor-alpha ; ren-shen-yang-rong-tang ; C-Reactive Protein (9007-41-4)
Language	English
Publishing date	2015-06
Publishing country	Scotland
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	1155895-7
ISSN	1873-6963 ; 0965-2299
ISSN (online)	1873-6963
ISSN	0965-2299
DOI	10.1016/j.ctim.2015.03.002
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Zs.A 3726: Show issues

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Article: Clinical efficacy of Rong-Yang-Jyh-Gan-Tang on patients with chronic hepatitis C: A double-blinded randomized placebo-controlled crossover study

Chen, Fang-Pey / Chien-Wei Su / Ching-Mao Chang / Jen-Lin Yang / Keng-Hsin Lan / Shinn-Jang Hwang / Shu-Chiung Chiang / Ta-Peng Wu / Yen-Ying Kung / Yi-Hsiang Huang

Journal of ethnopharmacology. 2017 Jan. 20, v. 196

2017

Abstract: ... response.We evaluated the efficacy of Rong-Yang-Jyh-Gan-Tang (RYJGT, composed of Long-Dan-Xie-Gan-Tang, Jia ...

Abstract	Traditional Chinese medicine (TCM) has been widely used by the Chinese population for treatment of chronic hepatitis. However, the efficacy of TCM for patients with chronic hepatitis has not been confirmed, mostly due to the lack of available scientific parameters such as serum viral load to evaluate treatment response.We evaluated the efficacy of Rong-Yang-Jyh-Gan-Tang (RYJGT, composed of Long-Dan-Xie-Gan-Tang, Jia-Wei-Xia-Yao-San, Dan-Shen, and Hou-Po) on patients with chronic hepatitis C.Thirty-six patients with chronic hepatitis C who had no response to or had contraindications to interferon-ribavirin therapy were randomly allocated to receive RYJGT 15g/day or placebo for 12 weeks. After a 2-week washout period, patients were crossed over to receive placebo or RYJGT for another 12 weeks. Evaluation parameters included liver biochemistries, serum HCVRNA, side effects of RYJGT/placebo, and TCM symptoms.Of the patients who had 12-week RYJGT treatment, 51.7% had decreased serum HCVRNA levels, whereas only 25.8% patients had decreased levels in the placebo group (p=0.036). TCM patterns of “Damp-Heat” and “Liver Qi Depression” had significantly improved after RYJGT treatment in comparison with the placebo. Logistic analyses showed that RYJGT treatment, and pre-treatment values of TCM symptoms of “Damp-Heat” and “Liver Qi Depression”, were statistically significant factors in predicting the decrease in serum HCVRNA.Chronic hepatitis C patients who received a 12-week RYJGT treatment had significantly higher HCVRNA decrease ratio, and improved TCM symptoms of “Damp-Heat” and “Liver Qi Depression”, than those who received the placebo. Our results require further larger scale clinical trials.
Keywords	adverse effects ; blood serum ; chronic hepatitis C ; clinical trials ; cross-over studies ; liver ; logit analysis ; Oriental traditional medicine ; patients ; placebos ; prediction ; therapeutics ; viral load
Language	English
Dates of publication	2017-0120
Size	p. 1-8.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2016.12.013
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The Quantitative Analysis of Land Use Structure Characteristics of County in Mountainous Areas in Sichuan Province of China-A Case Study of Rong County

Zhou, Wei / Wang, Chen / Song, Xing / Deng, Liang-ji

2011

Abstract: ... 2010 in Rong County, a mountainous region of Sichuan Province, by using quantitative geographical model ... we conduct quantitative analysis of the status quo of land use in Rong County in terms of land use ... characteristic of diversification of land use in Rong County, have low degree of centralization of land use and ...

Abstract	Based on me data concerning detailed survey on land in the year 2009 and land change in the year 2010 in Rong County, a mountainous region of Sichuan Province, by using quantitative geographical model, we conduct quantitative analysis of the status quo of land use in Rong County in terms of land use diversification, land use centralization, land use combination type, land use geographic significance and comprehensive use degree of land use. m e results show some characteristics as follows. Firstly, land use in study area displays prominent characteristic of diversification: there is a high degree of completeness land use type; there is a great similarity among towns. Secondly. there is limited combination number of land use type; mostly the combination number of the towns is farmland. Thirdly, the town with prominent characteristic of diversification of land use in Rong County, have low degree of centralization of land use and relatively big combination number of land use type, and vice versa. Fourthly, the type and quantity of agricultural land resources with geographical significance are relatively complete with nothing missing; it abounds in untapped land, but the overall index values of land use are smaller than 300, with low overall use degree of land.
Keywords	Land use ; Quantity structure ; Quantitative analysis ; Rong County ; Mountainous area of Sichuan Province ; China ; Agribusiness
Language	English
Publishing country	us
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book: Zhong yi mei rong da quan

Chen, Guiting

1989

Title translation	Compendium of cosmetics in Chinese traditional medicine.
Author's details	Chen Guiting ... [deng]
MeSH term(s)	Cosmetics ; Drugs, Chinese Herbal
Language	Chinese
Size	2 ,2, 583 p.
Edition	Di 1 ban.
Publisher	Zhongguo yi yao ke ji chu ban she
Publishing place	Beijing
Document type	Book
ISBN	9787506700382 ; 7506700387
Database	Catalogue of the US National Library of Medicine (NLM)

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Book: Fu rong niao (jing si niao) de si yang yu fan zhi

Chen, Heng / Zhao, Defu

2000

Title variant	Furongniao (jingsiniao) de siyang yu fan zhi
Author's details	Chen Heng, Zhao Defu bian zhu
Keywords	Canaries.
Language	Chinese
Size	2, 3 p., 4 p. of plates :, ill. (some col.) ;, 19 cm.
Edition	Di 1 ban.
Publisher	Jin dun chu ban she
Publishing place	Beijing
Document type	Book
ISBN	7508210433 ; 9787508210438
Database	NAL-Catalogue (AGRICOLA)

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Book ; Online: (Table 1) Radiolarian accumulation rates and geochemistry of ODP Site 184-1143, supplementary data to: Zhang, Lili; Chen, Muhong; Xiang, Rong; Zhang, Lanlan; Lu, Jun (2009): Productivity and continental denudation history from the South China Sea since the late Miocene. Marine Micropaleontology, 72(1-2), 76-85

Zhang, Lili / Chen, Muhong / Lu, Jun / Xiang, Rong / Zhang, Lanlan

2009

Abstract: Late Miocene-Recent micropaleontological and geochemical records from Ocean Drilling Program (ODP) Site 1143 in the southern South China Sea (SCS) indicate that increase and decrease in abundance of siliceous plankton may be controlled mainly by the ... ...

Abstract	Late Miocene-Recent micropaleontological and geochemical records from Ocean Drilling Program (ODP) Site 1143 in the southern South China Sea (SCS) indicate that increase and decrease in abundance of siliceous plankton may be controlled mainly by the input of nutrients derived from land and provided by upwelling. A high export production event - a "biogenic bloom" event - occurred in the southern SCS between 12 and 6 Ma. During this period, high ratios of smectite/(illite + chlorite), smectite/quartz and Al/K indicate a high weathering intensity of the Asian continent, possibly due to the intensification of the East Asian Summer Monsoon (EASM), which may have increased the net flux of nutrients to the ocean, both directly through terrestrial input and indirectly through upwelling activity. A drop in Ba/Ti, Al/Ti and Ca/Ti values around 6 Ma may indicate a lowering of productivity, possibly due to the large consumption of sea surface nutrients by the "biogenic bloom". Alternatively, it may indicate a shift in terrigenous input source area. At about 5.4 Ma, a decrease in weathering intensity, as indicated by a sudden decrease in the values of smectite/(illite + chlorite), smectite/quartz and Al/K, might have led to a sudden decrease of terrestrial nutrient input to the SCS. We suggest that the biogenic bloom ended when nutrients in surface waters were exhausted, because of a decrease in supply as well as a decrease in upwelling intensity due to weakening of the EASM. As a result, radiolarians were absent in the studied area between ~6 and 3.2 Ma. At ~3.2 Ma, radiolarians began to recover, possibly because the start of Northern Hemispheric glaciation and the rapid uplift of the Tibet Plateau led to intensification of the East Asian monsoon. After the Mid-Pleistocene Climate Transition at 0.9 Ma, the abundance and mass accumulation rates of radiolarians increased, probably as a result of increased upwelling activity driven by the increasing intensity of the summer monsoon.
Language	English
Dates of publication	2009-9999
Size	Online-Ressource
Publisher	PANGAEA - Data Publisher for Earth & Environmental Science
Publishing place	Bremen/Bremerhaven
Document type	Book ; Online
Note	This dataset is supplement to doi:10.1016/j.marmicro.2009.03.006
DOI	10.1594/PANGAEA.774606
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Book ; Online: Mechanical Properties of Advanced Multifunctional Coatings

Xu, Rong-Guang / Chen, Zhitong / Chen, Peijian / Peng, Guangjian / Chen, Zhitong

2023

Keywords	Technology: general issues ; History of engineering & technology ; Materials science ; ceramic matrix composite ; high temperature strength ; SiC fiber ; fiber-matrix interface coatings ; residual stress ; diamond-like carbon (DLC) ; plasma emission intensity ; magnetron sputtering ; tribological performance ; hot casting metals ; explosive compression bonding ; dovetail groove ; metallographic analysis ; mechanics analysis ; complex network theory ; sandstone ; network topology ; seepage ; distribution of degree ; structure of engineering coatings ; pore network model ; geometric distribution ; micropitting ; gear surface fatigue ; dedendum ; addendum ; SiO2 thin film ; nanoindentation ; surface morphology ; load-penetration curve ; hardness ; lubrication ; nanotribology ; phase transition ; adsorption ; Monte Carlo simulation ; molecular dynamics ; thermal barrier coatings ; thermal oxidation stress ; Cr3+ photoluminescence piezospectroscopy ; data processing method ; fitting function ; Lorentzian functions ; spectromechanics ; anti-reflection ; optical ; interference ; scratch ; damage ; CFD simulation ; ultraviolet nanoimprint lithography ; filling behavior ; moth-eye nanostructure ; thermal barrier coating ; terahertz time-domain spectroscopy (THz-TDS) ; stress optic coefficient ; barycenter method ; nanolaminates ; atomic layer deposition ; nanoengineering ; n/a
Language	English
Size	1 electronic resource (196 pages)
Publisher	MDPI - Multidisciplinary Digital Publishing Institute
Publishing place	Basel
Document type	Book ; Online
Note	English
HBZ-ID	HT030378395
ISBN	9783036566719 ; 3036566716
Database	ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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