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Article: Novel Host Response-Based Diagnostics to Differentiate the Etiology of Fever in Patients Presenting to the Emergency Department.

Atallah, Johnny / Ghebremichael, Musie / Timmer, Kyle D / Warren, Hailey M / Mallinger, Ella / Wallace, Ellen / Strouts, Fiona R / Persing, David H / Mansour, Michael K

Diagnostics (Basel, Switzerland)

2023 Volume 13, Issue 5

Abstract: Fever is a common presentation to urgent-care services and is linked to multiple disease processes. To rapidly determine the etiology of fever, improved diagnostic modalities are necessary. This prospective study of 100 hospitalized febrile patients ... ...

Abstract	Fever is a common presentation to urgent-care services and is linked to multiple disease processes. To rapidly determine the etiology of fever, improved diagnostic modalities are necessary. This prospective study of 100 hospitalized febrile patients included both positive (FP) and negative (FN) subjects in terms of infection status and 22 healthy controls (HC). We evaluated the performance of a novel PCR-based assay measuring five host mRNA transcripts directly from whole blood to differentiate infectious versus non-infectious febrile syndromes as compared to traditional pathogen-based microbiology results. The FP and FN groups observed a robust network structure with a significant correlation between the five genes. There were statistically significant associations between positive infection status and four of the five genes: IRF-9 (OR = 1.750, 95% CI = 1.16-2.638), ITGAM (OR = 1.533, 95% CI = 1.047-2.244), PSTPIP2 (OR = 2.191, 95% CI = 1.293-3.711), and RUNX1 (OR = 1.974, 95% CI = 1.069-3.646). We developed a classifier model to classify study participants based on these five genes and other variables of interest to assess the discriminatory power of the genes. The classifier model correctly classified more than 80% of the participants into their respective groups, i.e., FP or FN. The GeneXpert prototype holds promise for guiding rapid clinical decision-making, reducing healthcare costs, and improving outcomes in undifferentiated febrile patients presenting for urgent evaluation.
Language	English
Publishing date	2023-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics13050953
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Real-time Screening of Specimen Pools for Coronavirus Disease 2019 (COVID-19) Infection at Sanya Airport, Hainan Island, China.

Li, Huiling / Sun, Kai / Persing, David H / Tang, Yi-Wei / Shen, Dingxia

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

2020 Volume 73, Issue 2, Page(s) 318–320

Abstract: A 10:1 pooled test strategy on-site at an airport of China was pursued, resulting in increased test throughput, limited use of reagents, and increased testing efficiency without loss of sensitivity. This testing approach has the potential to reduce the ... ...

Abstract	A 10:1 pooled test strategy on-site at an airport of China was pursued, resulting in increased test throughput, limited use of reagents, and increased testing efficiency without loss of sensitivity. This testing approach has the potential to reduce the need for contact tracing when the results are delivered first time.
MeSH term(s)	Airports ; COVID-19 ; China/epidemiology ; Contact Tracing ; Humans ; Mass Screening ; SARS-CoV-2
Keywords	covid19
Language	English
Publishing date	2020-09-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1099781-7
ISSN	1537-6591 ; 1058-4838
ISSN (online)	1537-6591
ISSN	1058-4838
DOI	10.1093/cid/ciaa1074
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Article ; Online: Novel Host Response-Based Diagnostics to Differentiate the Etiology of Fever in Patients Presenting to the Emergency Department

Johnny Atallah / Musie Ghebremichael / Kyle D. Timmer / Hailey M. Warren / Ella Mallinger / Ellen Wallace / Fiona R. Strouts / David H. Persing / Michael K. Mansour

Diagnostics, Vol 13, Iss 953, p

2023 Volume 953

Abstract	Fever is a common presentation to urgent-care services and is linked to multiple disease processes. To rapidly determine the etiology of fever, improved diagnostic modalities are necessary. This prospective study of 100 hospitalized febrile patients included both positive (FP) and negative (FN) subjects in terms of infection status and 22 healthy controls (HC). We evaluated the performance of a novel PCR-based assay measuring five host mRNA transcripts directly from whole blood to differentiate infectious versus non-infectious febrile syndromes as compared to traditional pathogen-based microbiology results. The FP and FN groups observed a robust network structure with a significant correlation between the five genes. There were statistically significant associations between positive infection status and four of the five genes: IRF-9 (OR = 1.750, 95% CI = 1.16–2.638), ITGAM (OR = 1.533, 95% CI = 1.047–2.244), PSTPIP2 (OR = 2.191, 95% CI = 1.293–3.711), and RUNX1 (OR = 1.974, 95% CI = 1.069–3.646). We developed a classifier model to classify study participants based on these five genes and other variables of interest to assess the discriminatory power of the genes. The classifier model correctly classified more than 80% of the participants into their respective groups, i.e., FP or FN. The GeneXpert prototype holds promise for guiding rapid clinical decision-making, reducing healthcare costs, and improving outcomes in undifferentiated febrile patients presenting for urgent evaluation.
Keywords	host-response ; diagnostics ; gene signatures ; fever ; febrile syndromes ; Medicine (General) ; R5-920
Subject code	610
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Predictive Factors of Outcomes in Helmet Therapy for Deformational Plagiocephaly and Brachycephaly.

Hauc, Sacha C / Long, Aaron S / Rivera, Jean Carlo / Ihnat, Jacqueline / Littlefield, Timothy R / Shah, Hemali P / Pondugula, Nishita / Junn, Adam H / Almeida, Mariana N / Alper, David / Persing, John / Alperovich, Michael

The Journal of craniofacial surgery

2022 Volume 34, Issue 1, Page(s) 231–234

Abstract: Deformational plagiocephaly and brachycephaly, or abnormal flattening of the infant skull due to external forces, are often managed with orthotic helmet therapy. Although helmet therapy is widely used, the factors that predict poor outcomes are not well ... ...

Abstract	Deformational plagiocephaly and brachycephaly, or abnormal flattening of the infant skull due to external forces, are often managed with orthotic helmet therapy. Although helmet therapy is widely used, the factors that predict poor outcomes are not well characterized. In this study of over 140,000 patients who received helmet therapy, older age and greater severity at presentation, and noncompliance with treatment were each independently associated with worse craniometric and provider-reported outcomes. Each additional point of cranial vault asymmetry index or cephalic index at a presentation is associated with an increased likelihood of residual brachycephaly at completion [odds ratio (OR): 1.067; 95% Cl: 1.058-1.075; P <0.0001 and OR: 2.043; 95% CI: 2.021-2.065; P <0.0001, respectively], whereas each additional point of cranial vault asymmetry index at a presentation associated with increased likelihood of residual asymmetry at completion (OR: 2.148; 95% Cl: 2.118-2.179; P <0.0001). Patients were more likely to have residual brachycephaly or asymmetry with increasing age at treatment initiation (OR: 1.562; 95% CI: 1.524-1.600; P <0.0001 and OR: 1.673; 95% Cl: 1.634-1.713; P <0.0001, respectively, for each additional month of age at initiation). These results highlight a need for prompt referral for helmeting, especially in cases with severe features or when patients present late to care. Potentially modifiable factors are age at helmeting and compliance with treatment protocols, and consideration of these factors may be important for achieving success in some cases.
MeSH term(s)	Infant ; Humans ; Plagiocephaly, Nonsynostotic/therapy ; Treatment Outcome ; Head Protective Devices ; Skull ; Craniosynostoses/therapy ; Orthotic Devices ; Plagiocephaly
Language	English
Publishing date	2022-10-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	1159501-2
ISSN	1536-3732 ; 1049-2275
ISSN (online)	1536-3732
ISSN	1049-2275
DOI	10.1097/SCS.0000000000009048
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Zs.A 3761: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 175: Show issues

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Article ; Online: The SARS-CoV-2 Outbreak: Diagnosis, Infection Prevention, and Public Perception.

Wang, Ping / Anderson, Neil / Pan, Yang / Poon, Leo / Charlton, Carmen / Zelyas, Nathan / Persing, David / Rhoads, Daniel / Babcock, Hilary

Clinical chemistry

2020

Keywords	covid19
Language	English
Publishing date	2020-03-10
Publishing country	England
Document type	Journal Article
ZDB-ID	80102-1
ISSN	1530-8561 ; 0009-9147
ISSN (online)	1530-8561
ISSN	0009-9147
DOI	10.1093/clinchem/hvaa080
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Article ; Online: Activating human epidermal growth factor receptor 2 (HER2) gene mutation in bone metastases from breast cancer.

Christgen, Matthias / Bartels, Stephan / Luft, Angelina / Persing, Sascha / Henkel, Daniel / Lehmann, Ulrich / Kreipe, Hans

Virchows Archiv : an international journal of pathology

2018 Volume 473, Issue 5, Page(s) 577–582

Abstract: In addition to amplification, point mutations of the human epidermal growth factor receptor 2 (HER2) gene (ERBB2) have been shown to activate the corresponding signaling pathway in breast cancer. The prevalence of ERBB2/HER2 mutation in bone metastasis ... ...

Abstract	In addition to amplification, point mutations of the human epidermal growth factor receptor 2 (HER2) gene (ERBB2) have been shown to activate the corresponding signaling pathway in breast cancer. The prevalence of ERBB2/HER2 mutation in bone metastasis of breast cancer and the associated phenotype are not known. In this study, bone metastases from breast cancer patients (n = 231) were analyzed for ERBB2/HER2 mutation. In 7 patients (3%; median age 70 years, range 50-83 years), gain-of-function mutations of ERBB2/HER2 were detected. The most frequent mutation was p.L755S (71%). In 29% of mutated cases, p.V777L was found. Lobular breast cancer was present in 71% of mutated cases (n = 5) and in 49% of all samples (n = 231; p = 0.275). Mutation frequency was 4.4% in the lobular subgroup and 17.4% in the pleomorphic subtype of lobular cancer (n = 23), respectively. All but one mutated lobular cancers were of the pleomorphic subtype (p = 0.006). Mutated cancers belonged either to the luminal (n = 4) or to the triple-negative types (n = 3). With regard to protein expression and gene amplification, HER2 was negative in all mutated cases. Among the 14% of metastatic luminal cancers with estrogen receptor gene (ESR1) mutation, conveying resistance against aromatase inhibitors, no concomitant ERBB2/HER2 mutation occurred. We conclude that activating HER2 mutation is present in about 3% of bone metastases from breast cancers, with significantly higher rates in the pleomorphic subtype of lobular cancer. Since mutated cases appear to be HER2-negative by conventional testing, the opportunity for specific anti-HER2 therapy may be missed.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Bone Neoplasms/genetics ; Bone Neoplasms/secondary ; Breast Neoplasms/genetics ; Breast Neoplasms/pathology ; Carcinoma, Lobular/genetics ; Carcinoma, Lobular/secondary ; Female ; Gain of Function Mutation ; Gene Expression Regulation, Neoplastic ; Humans ; Middle Aged ; Point Mutation ; Receptor, ErbB-2/genetics ; Retrospective Studies
Chemical Substances	ERBB2 protein, human (EC 2.7.10.1) ; Receptor, ErbB-2 (EC 2.7.10.1)
Language	English
Publishing date	2018-08-09
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1184867-4
ISSN	1432-2307 ; 0945-6317
ISSN (online)	1432-2307
ISSN	0945-6317
DOI	10.1007/s00428-018-2414-1
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Article: Real-time Screening of Specimen Pools for Coronavirus Disease 2019 (COVID-19) Infection at Sanya Airport, Hainan Island, China

Li, Huiling / Sun, Kai / Persing, David H / Tang, Yi-Wei / Shen, Dingxia

Clin. infect. dis

Abstract	A 10:1 pooled test strategy on-site at an airport of China was pursued, resulting in increased test throughput, limited use of reagents, and increased testing efficiency without loss of sensitivity. This testing approach has the potential to reduce the need for contact tracing when the results are delivered first time.
Keywords	covid19
Publisher	WHO
Document type	Article
Note	WHO #Covidence: #805320
Database	COVID19

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Article ; Online: Real-time Screening of Specimen Pools for Coronavirus Disease 2019 (COVID-19) Infection at Sanya Airport, Hainan Island, China

Li, Huiling / Sun, Kai / Persing, David H / Tang, Yi-Wei / Shen, Dingxia

Clinical Infectious Diseases ; ISSN 1058-4838 1537-6591

2020

Abstract: Abstract A 10:1 pooled test strategy on-site at an airport of China was pursued, resulting in increased test throughput, limited use of reagents, and increased testing efficiency without loss of sensitivity. This testing approach has the potential to ... ...

Abstract	Abstract A 10:1 pooled test strategy on-site at an airport of China was pursued, resulting in increased test throughput, limited use of reagents, and increased testing efficiency without loss of sensitivity. This testing approach has the potential to reduce the need for contact tracing when the results are delivered first time.
Keywords	Microbiology (medical) ; Infectious Diseases ; covid19
Language	English
Publisher	Oxford University Press (OUP)
Publishing country	uk
Document type	Article ; Online
DOI	10.1093/cid/ciaa1074
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Whither the chips may fall?

Persing, D H

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

1999 Volume 4, Issue 4, Page(s) 389

MeSH term(s)	Diagnostic Techniques and Procedures ; Gene Expression Profiling ; Humans ; Oligonucleotide Array Sequence Analysis ; Periodicals as Topic ; Polymerase Chain Reaction
Language	English
Publishing date	1999-12
Publishing country	United States
Document type	Letter
ZDB-ID	1405760-8
ISSN	1084-8592
ISSN	1084-8592
DOI	10.1016/s1084-8592(99)80015-0
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Article ; Online: The Xpert MTB/RIF Ultra assay detects Mycobacterium tuberculosis complex DNA in white rhinoceros (Ceratotherium simum) and African elephants (Loxodonta africana).

Goosen, Wynand J / Kerr, Tanya J / Kleynhans, Léanie / Warren, Robin M / van Helden, Paul D / Persing, David H / Parsons, Sven D C / Buss, Peter / Miller, Michele A

Scientific reports

2020 Volume 10, Issue 1, Page(s) 14482

Abstract: The study describes the novel use of the Xpert MTB/RIF Ultra assay for detection of Mycobacterium tuberculosis complex (MTBC) DNA in samples from white rhinoceros (Ceratotherium simum) and African elephants (Loxodonta africana). Culture negative ... ...

Abstract	The study describes the novel use of the Xpert MTB/RIF Ultra assay for detection of Mycobacterium tuberculosis complex (MTBC) DNA in samples from white rhinoceros (Ceratotherium simum) and African elephants (Loxodonta africana). Culture negative respiratory sample matrices were spiked to determine if the Ultra could detect MTBC DNA in rhinoceros and elephant samples. Rhinoceros bronchial alveolar lavage fluid (BALF) was found to have an inhibitory effect on the Ultra. In this study, the limit of detection (LOD) of M. tuberculosis H37Rv in all spiked animal samples were 2 CFU/ml compared to 15.6 CFU/ml for humans, while the LOD for M. bovis SB0121 was 30 CFU/ml compared to 143.4 CFU/ml for M. bovis BCG in humans. Screening was performed on stored tissue and respiratory samples from known MTBC-infected animals and MTBC DNA was detected in 92% of samples collected from six rhinoceros and two elephants. Conversely, 83% of culture-negative tissue and respiratory samples from uninfected animals tested negative on the Ultra. In conclusion, the Ultra assay appears to be a sensitive and rapid diagnostic test for the detection of MTBC DNA from tissue and respiratory samples collected from African elephants and rhinoceros. Furthermore, the Ultra assay could provide a new tool for the detection of MTBC in various sample types from other wildlife species.
MeSH term(s)	Animals ; Antibiotics, Antitubercular/pharmacology ; Biological Assay ; Bronchoalveolar Lavage Fluid ; DNA, Bacterial/isolation & purification ; Diagnostic Tests, Routine/veterinary ; Elephants/microbiology ; Humans ; Limit of Detection ; Mycobacterium bovis ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Perissodactyla/microbiology ; Reproducibility of Results ; Rifampin/pharmacology ; Sensitivity and Specificity ; Sputum/microbiology
Chemical Substances	Antibiotics, Antitubercular ; DNA, Bacterial ; Rifampin (VJT6J7R4TR)
Language	English
Publishing date	2020-09-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-71568-9
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