LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 3 of total 3

Search options

  1. Article: In vitro antibiotic susceptibility of Bacteroides fragilis strains isolated from excised appendix of patients with phlegmonous or gangrenous appendicitis.

    Wójcik-Stojek, B / Bulanda, M / Martirosian, G / Heczko, P / Meisel-Mikołajczyk, F

    Acta microbiologica Polonica

    2000  Volume 49, Issue 2, Page(s) 171–175

    Abstract: ... of anaerobes were isolated. The antibiotic susceptibility of 30 isolated B. fragilis strains was tested using E ...

    Abstract Out of 34 studied after-appendectomy tissues of adult and child patients 86 different strains of anaerobes were isolated. The antibiotic susceptibility of 30 isolated B. fragilis strains was tested using E tests. All studied strains were sensitive to imipenem, clindamycin and penicillin/tazobactam. Sensitivity to penicillin and cefoxitin was variable among these strains. One strain resistant to metronidazole (MIC--256 mg/L) and 3 strains with increased MIC to metronidazole were detected. Most of isolated strains were beta-lactamase producers.
    MeSH term(s) Adult ; Appendicitis/microbiology ; Appendicitis/surgery ; Appendix/microbiology ; Bacteroides fragilis/drug effects ; Bacteroides fragilis/isolation & purification ; Child ; Drug Resistance, Microbial ; Humans ; Metronidazole/pharmacology ; Microbial Sensitivity Tests
    Chemical Substances Metronidazole (140QMO216E)
    Language English
    Publishing date 2000
    Publishing country Poland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 219906-3
    ISSN 0137-1320 ; 0001-6195 ; 0567-7807
    ISSN 0137-1320 ; 0001-6195 ; 0567-7807
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 962: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Acute appendicitis: the role of enterotoxigenic strains of Bacteroides fragilis and Clostridium difficile.

    Martirosian, G / Bulanda, M / Wójcik-Stojek, B / Obuch-Woszczatyński, P / Rouyan, G / Heczko, P / Meisel-Mikołajczyk, F

    Medical science monitor : international medical journal of experimental and clinical research

    2001  Volume 7, Issue 3, Page(s) 382–386

    Abstract: ... enterotoxin-producing B. fragilis strains and toxigenic C. difficile strains and the pathogenesis of acute ... the B. fragilis group was most frequently isolated: 34 B. fragilis strains were cultured from 21 post ... appendectomy tissues. Two enterotoxin-producing B. fragilis strains were found. Enterotoxin titers (1:10 and 1 ...

    Abstract Background: The aim of this study was to investigate whether there is a relationship between enterotoxin-producing B. fragilis strains and toxigenic C. difficile strains and the pathogenesis of acute appendicitis.
    Material and methods: Post-appendectomy tissues from 34 patients with histopathologically confirmed phlegmonous or gangrenous appendicitis were studied.
    Results: Among 86 anaerobes isolated, the B. fragilis group was most frequently isolated: 34 B. fragilis strains were cultured from 21 post-appendectomy tissues. Two enterotoxin-producing B. fragilis strains were found. Enterotoxin titers (1:10 and 1:160, respectively) were measured on HT29/C cells. The presence of the enterotoxin gene was confirmed by PCR in DNA extracted from both strains. Among 21 DNA samples isolated from those post-appendectomy tissues from which B. fragilis strains were cultured, the presence of the enterotoxin gene was confirmed in only one case (the corresponding B. fragilis strain enterotoxin titer was 1:160). A unique toxigenic C. difficile strain was also cultured from the tissue of an adult patient with gangrenous non-perforated appendicitis. The presence of toxin A and toxin B genes was confirmed by PCR in DNA extracted from the C. difficile strain, but these genes were not found in the DNA extracted from the corresponding tissue.
    Conclusion: The presence of enterotoxigenic B. fragilis and toxigenic C. difficile strains was shown in post-appendectomy tissue from patients with phlegmonous and gangrenous appendicitis, and the B. fragilis enterotoxin gene was detected directly in the corresponding tissue. Further investigations (including immunologic aspects) require to confirm the role of these toxins in pathogenesis of acute appendicitis.
    MeSH term(s) Adult ; Appendicitis/microbiology ; Bacteroides fragilis/pathogenicity ; Child ; Clostridium difficile/pathogenicity ; Enzyme-Linked Immunosorbent Assay ; Humans ; Intestinal Perforation/microbiology ; Polymerase Chain Reaction ; Rupture, Spontaneous/microbiology
    Language English
    Publishing date 2001-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1439041-3
    ISSN 1643-3750 ; 1234-1010
    ISSN (online) 1643-3750
    ISSN 1234-1010
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4924: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (eastern Poland).

    Cisak, Ewa / Wójcik-Fatla, Angelina / Stojek, Nimfa / Chmielewska-Badora, Jolanta / Zwoliński, Jacek / Buczek, Alicja / Dutkiewicz, Jacek

    Annals of agricultural and environmental medicine : AAEM

    2006  Volume 13, Issue 2, Page(s) 301–306

    Abstract: ... lato (B.b.s.l.) by polymerase chain reaction (PCR). Another group of 438 I. ricinus ticks collected ... from 4 districts were examined for the presence of B.b.s.l. by culture on BSKH liquid medium confirmed ... by nested-PCR in 113 ticks lysates showing presence of B.b.s.l. (in PCR or in culture and PCR). 5.4 ...

    Abstract The objective of the study was to determine the prevalence rate of 3 Borrelia burgdorferi genospecies in Ixodes ricinus ticks collected from wooded areas of the Lublin region (eastern Poland). A group of 1,813 I. ricinus ticks from 6 districts were examined for the presence of Borrelia burgdorferi sensu lato (B.b.s.l.) by polymerase chain reaction (PCR). Another group of 438 I. ricinus ticks collected from 4 districts were examined for the presence of B.b.s.l. by culture on BSKH liquid medium confirmed by PCR, and for the presence of Borrelia spp. by dark field microscopy (DFM). Borrelia burgdorferi genospecies (Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii) were determined by nested-PCR in 113 ticks lysates showing presence of B.b.s.l. (in PCR or in culture and PCR). 5.4 % of I. ricinus ticks examined by PCR showed the presence of B.b.s.l. DNA. The infection rate was highest in females (12.1 %), lower in males (6.0 %) and the lowest in nymphs (1.7 %) (p < 0.001). The minimum infection rate of I. ricinus ticks with B.b.s.l. determined by culture was 3.4 %, whereas the minimum infection rate of ticks with motile spirochetes morphologically resembling Borrelia spp., determined by DFM, amounted to 11.2 %. The presence of all 3 Borrelia burgdorferi genospecies under investigation was found in ticks collected from 5 out of 6 examined districts. In 81.4 % of infected ticks only single infection with 1 genospecies was observed, while coinfections with 2 or 3 genospecies were detected respectively in 16.8 % and 1.8 % of infected ticks. Borrelia burgdorferi sensu stricto was the dominant genospecies in all examined tick stages and districts, both in single infections and in coinfections, and found in a total of 62.8 % of I. ricinus ticks infected with B.b.s.l. Borrelia afzelii and Borrelia garinii were less frequent and observed in respectively 39.8 % and 17.8 % of infected ticks.
    MeSH term(s) Animals ; Arachnid Vectors/microbiology ; Arachnid Vectors/parasitology ; Borrelia burgdorferi Group/classification ; Borrelia burgdorferi Group/genetics ; Borrelia burgdorferi Group/isolation & purification ; DNA, Bacterial/genetics ; Environmental Monitoring/methods ; Epidemiological Monitoring ; Female ; Ixodes/microbiology ; Ixodes/parasitology ; Larva/microbiology ; Lyme Disease/epidemiology ; Lyme Disease/microbiology ; Male ; Nymph/microbiology ; Poland/epidemiology ; Polymerase Chain Reaction ; Prevalence ; Species Specificity
    Chemical Substances DNA, Bacterial
    Language English
    Publishing date 2006
    Publishing country Poland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1215115-4
    ISSN 1898-2263 ; 1232-1966
    ISSN (online) 1898-2263
    ISSN 1232-1966
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4242: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top