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Article ; Online: Mesoderm Induction during Early Amphibian Development: (mesoderm induction/growth factor/bFGF, activin/gene expression/organizer).

Asashima, Makoto

2023 Volume 36, Issue 4, Page(s) 343–355

Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.1994.00343.x
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Article ; Online: Spemann-Mangold organizer and mesoderm induction.

Asashima, Makoto / Satou-Kobayashi, Yumeko

Cells & development

2024 , Page(s) 203903

Abstract: The discovery of the Spemann-Mangold organizer strongly influenced subsequent research on embryonic induction, with research aiming to elucidate the molecular characteristics of organizer activity being currently underway. Herein, we review the history ... ...

Abstract	The discovery of the Spemann-Mangold organizer strongly influenced subsequent research on embryonic induction, with research aiming to elucidate the molecular characteristics of organizer activity being currently underway. Herein, we review the history of research on embryonic induction, and describe how the mechanisms of induction phenomena and developmental processes have been investigated. Classical experiments investigating the differentiation capacity and inductive activity of various embryonic regions were conducted by many researchers, and important theories of region-specific induction and the concept for chain of induction were proposed. The transition from experimental embryology to developmental biology has enabled us to understand the mechanisms of embryonic induction at the molecular level. Consequently, many inducing substances and molecules such as transcriptional factors and peptide growth factors involved in the organizer formation were identified. One of peptide growth factors, activin, acts as a mesoderm- and endoderm-inducing substance. Activin induces several tissues and organs from the undifferentiated cell mass of amphibian embryos in a concentration-dependent manner. We review the extent to which we can control in vitro organogenesis from undifferentiated cells, and discuss the application to stem cell-based regenerative medicine based on insights gained from animal experiments, such as in amphibians.
Language	English
Publishing date	2024-02-01
Publishing country	Netherlands
Document type	Journal Article
ISSN	2667-2901
ISSN (online)	2667-2901
DOI	10.1016/j.cdev.2024.203903
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Correction: The Influence of Human Connections and Collaboration on Research Grant Success at Various Career Stages: Regression Analysis.

Hashiguchi, Akiko / Asashima, Makoto / Takahashi, Satoru

JMIR formative research

2024 Volume 8, Page(s) e58733

Abstract: This corrects the article DOI: 10.2196/49905.]. ...

Abstract	[This corrects the article DOI: 10.2196/49905.].
Language	English
Publishing date	2024-04-05
Publishing country	Canada
Document type	Published Erratum
ISSN	2561-326X
ISSN (online)	2561-326X
DOI	10.2196/58733
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Article ; Online: Sequential gene expression during pronephric tubule formation in vitro in Xenopus ectoderm.

Uochi, Takaaki / Asashima, Makoto

Development, growth & differentiation

2023 Volume 38, Issue 6, Page(s) 625–634

Abstract: The kidney has been used as a model organ to analyze organogenesis. In in vitro experiments using Xenopus blastula ectoderm, the development of pronephric tubules (the prototype of the kidney) may be induced by treatment with activin A and retinoic acid ( ...

Abstract	The kidney has been used as a model organ to analyze organogenesis. In in vitro experiments using Xenopus blastula ectoderm, the development of pronephric tubules (the prototype of the kidney) may be induced by treatment with activin A and retinoic acid (RA). The present study examined whether pronephric tubules induced in ectodermal explants exhibited similar characteristics to those of normal embryos at the molecular level. The experimental conditions required for high frequency induction (100%) of pronephric tubule formation from presumptive ectoderm without the development of muscle and notochord were determined. The developmental expression of the pronephros marker genes Xlim-1 and Xlcaax-1 was examined in induced pronephric tubules. After treatment with 10 ng/mL activin A and 10
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1046/j.1440-169X.1996.t01-5-00006.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 194: Show issues

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Article ; Online: CHANGES IN THE ELECTROPHORETIC MOBILITY OF PRESUMPTIVE ECTODERM CELLS TREATED WITH MESODERMAL INDUCING SUBSTANCES.

Togashi, Shin / Asashima, Makoto

Development, growth & differentiation

2023 Volume 22, Issue 5, Page(s) 797–803

Abstract: Using gastrula ectoderm of Cynops pyrrhogaster, the electrophoretic mobility (EPM) of cells was measured. Before EPM measurement, the cells were treated with either LiCl or swimbladder tissue, which are known to possess endo- and mesodermal inducing ... ...

Abstract	Using gastrula ectoderm of Cynops pyrrhogaster, the electrophoretic mobility (EPM) of cells was measured. Before EPM measurement, the cells were treated with either LiCl or swimbladder tissue, which are known to possess endo- and mesodermal inducing activity. The EPM of the treated cells increased after 48 hr. A significant difference was observed between the control and experimental series. In addition to the measurements of EPM, histological sections of the explants were examined. Before obvious tissue differentiation occurred in the explants, definitive changes in the EPM values were recorded at 48 and 72 hr after treatment.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.1980.00797.x
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Zs.A 194: Show issues

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Article ; Online: Mesoderm and Neural Inductions on Newt Ectoderm by Activin A: (mesoderm induction/newt embryo/activin/EDF).

Moriya, Naomi / Asashima, Makoto

Development, growth & differentiation

2023 Volume 34, Issue 5, Page(s) 589–594

Abstract: Mesoderm-inducing activity of human recombinant activin A was examined on presumptive ectoderm of the Japanese newt, Cynops pyrrhogaster, by using the animal cap assay, Activin A induced neural tissues and mesodermal tissues such as brain, neural tube, ... ...

Abstract	Mesoderm-inducing activity of human recombinant activin A was examined on presumptive ectoderm of the Japanese newt, Cynops pyrrhogaster, by using the animal cap assay, Activin A induced neural tissues and mesodermal tissues such as brain, neural tube, notochord, muscle, mesenchyme, coelomic epithelium and blood-like cells after 14 days cultivation. These tissues were induced by activin A at concentrations ranging from 0.5- 100 ng/ml. Dose-dependent inducing activity of activity A on newt ectoderm was slightly different from that on other animals, including Xenopus. Wide range of concentration of activin A (0.5- 100 ng/ml) could induce the neural tube, notochord, mesenchyme and coelomic epithelium on the newt ectoderm. Though the percentage of induced explants (two out of 23 explants, 8.7%) was low, the pulsating heart was induced. This paper showed first that activin could induce the mesodermal and neural tissues in newt presumptive ectoderm. Since activin homologues were present In Xenopus and chick embryos, it is likely that activin may be one of the natural inducers in a wide range of species.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.1992.00589.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 194: Show issues

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Article ; Online: Structural Changes of Yolk Platelets and Related Organelles during Development of the Newt Embryo: (amphibia/early development/electron microscopy/yolk platelet/cytoplasmic organelle).

Komazaki, Shinji / Asashima, Makoto

Development, growth & differentiation

2023 Volume 29, Issue 4, Page(s) 323–331

Abstract: Structural changes in yolk platelets and related organelles in the cytoplasm of the presumptive ectodermal region up to the stage of gastrulation were studied by light and electron microscopies using full-grown oocytes, mature eggs descending the oviduct ...

Abstract	Structural changes in yolk platelets and related organelles in the cytoplasm of the presumptive ectodermal region up to the stage of gastrulation were studied by light and electron microscopies using full-grown oocytes, mature eggs descending the oviduct and embryos of the newt, Cynops pyrrhogaster. Yolk platelets with a superficial layer are first observed in mature eggs descending the oviduct. During the cleavage and early morula stages, the superficial layer increases in thickness and the main bodies become more slender. The superficial layer decreases in thickness in the blastula stage, and many yolk platelets lose this layer in the gastrula stage. The amount of rough-surfaced endoplasmic reticulum (r-ER) increases rapidly in the morula stage, while Golgi complexes gradually increase in number between the cleavage and gastrula stages. In the cleavage and early morula stages, most of the r-ER is closely adherent to yolk platelets and is associated with several mitochondria. Two types of free vesicles, large (0.5-4.0 μm diameter) and small (0.15-0.3 μm diameter), were seen in abundance from the early morula stage to the early gastrula stages. Changes in the structure of yolk platelets are discussed in relation to changes in other cytoplsmic organelles.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.1987.00323.x
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Zs.A 194: Show issues

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Article ; Online: In Vitro Control of the Embryonic Form of Xenopus laevis by Activin A: Time and Dose-Dependent Inducing Properties of Activin-Treated Ectoderm: (activin/ectoderm/organizer/Xenopus laevis/neural induction).

Ariizumi, Takashi / Asashima, Makoto

Development, growth & differentiation

2023 Volume 36, Issue 5, Page(s) 499–507

Abstract: The inducing properties of activin-treated ectoderm of Xenopus laevis were examined by the preculture and sandwich culture methods. Presumptive ectodermal sheets of the late blastula were treated with 10-100 ng/ml of activin A and precultured for 0-7 hr ... ...

Abstract	The inducing properties of activin-treated ectoderm of Xenopus laevis were examined by the preculture and sandwich culture methods. Presumptive ectodermal sheets of the late blastula were treated with 10-100 ng/ml of activin A and precultured for 0-7 hr in Steinberg's solution. They were then sandwiched between two sheets of ectoderm from other late blastulae. Ectoderm precultured for a short term induced trunk-tail structures, whereas that precultured for a long term induced head structures in addition to trunk-tail structures. These time-dependent changes in inducing properties occurred more rapidly when the concentration of activin A was higher. These results suggest that the activin-treated ectoderm functioned as a "head organizer" or "trunk-tail organizer" depending upon the concentration of activin A and the duration of preculture. To trace the cell lineage of the sandwich explants, activin-treated ectoderm labeled with fluorescein-dextran-amine (FDA) was used in this study. The explants sandwiching the long term-precultured ectoderm formed head structures equipped with non-labeled neural tissues (brain and eye) as well as FDA-labeled mesodermal tissues. These results suggest that the activin-treated ectoderm mainly differentiates into mesodermal tissues and induces neural tissues as the organizer does in normal development.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1111/j.1440-169X.1994.00499.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 194: Show issues

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Article ; Online: The Influence of Human Connections and Collaboration on Research Grant Success at Various Career Stages: Regression Analysis.

Hashiguchi, Akiko / Asashima, Makoto / Takahashi, Satoru

JMIR formative research

2024 Volume 8, Page(s) e49905

Abstract: Background: Documenting the grant acquisition characteristics of a highly selective group of researchers could provide insights into the research and faculty development of talented individuals, and the insights gained to foster such researchers will ... ...

Abstract	Background: Documenting the grant acquisition characteristics of a highly selective group of researchers could provide insights into the research and faculty development of talented individuals, and the insights gained to foster such researchers will help university management strengthen their research capacity. Objective: This study examines the role of human connections in the success of biomedical researchers in Japanese universities. Methods: This study used grant data from the Grants-in-Aid for Scientific Research (GIA) program, the largest competitive research funding program in Japan, to collect information on projects and their implementation systems obtained throughout the participants' careers. Grant success was measured by the number and amounts of the awards obtained while participants occupied the role of principal investigator. Human connections were quantified by the number of projects in which the participants took part as members and were classified by their relationship with the project leader. Data were matched with information on career history, publication performance, and experience of the participants with government-funded programs apart from GIA and were analyzed using univariate and multivariate regression analyses. Results: Early-career interpersonal relationships, as measured using the h-index value of the researchers who provided the participants with their initial experience as project members, had a positive effect on grant success. The experience of contributing to prestigious research programs led by top researchers dramatically increased the cumulative amount of GIA awards received by the participants over time. Univariate logistic regression analyses revealed that more interactions with upper-level researchers resulted in fewer acquisitions of large programs (odds ratio [OR] 0.67, 95% CI 0.50-0.89). Collaboration with peers increased the success rate of ≥2 research grants in large programs in situations in which both the participant and project leader were professors (OR 1.16, 95% CI 1.06-1.26). Tracking the process of research development, we found that collaboration during the periods of 10 to 14 years and 15 to 19 years after completing a doctorate degree determined the size of the project that the participant would obtain-interactions with peer researchers and subordinates during the 10- to 14-year postdegree period had positive effects on ≥2 large-program acquisitions (OR 1.51, 95% CI 1.09-2.09 and OR 1.31, 95% CI 1.10-1.57, respectively), whereas interactions with subordinates during the 15- to 19-year postdegree period also had positive effects (OR 1.25, 95% CI 1.25-1.07). Furthermore, relationships that remained narrowly focused resulted in limited grant success for small programs. Conclusions: Human networking is important for improving an individual's ability to obtain external funding. The results emphasize the importance of having a high-h-indexed collaborator to obtain quality information early in one's career; working with diverse, nonsupervisory personnel at the midcareer stage; and engaging in synergistic collaborations upon establishing a research area in which one can take more initiatives.
Language	English
Publishing date	2024-02-28
Publishing country	Canada
Document type	Journal Article
ISSN	2561-326X
ISSN (online)	2561-326X
DOI	10.2196/49905
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Onset of competence to respond to activin A in isolated eight-cell stage Xenopus animal blastomeres.

Kinoshita, Kei / Bessho, Tomoko / Asashima, Makoto

Development, growth & differentiation

2023 Volume 37, Issue 3, Page(s) 303–309

Abstract: Single animal hemisphere blastomeres isolated from the eight-cell stage Xenopus embryos differentiate into mesoderm when treated with activin A, whereas when cultured without activin they form atypical epidermis. The mesoderm tissue induced by activin is ...

Abstract	Single animal hemisphere blastomeres isolated from the eight-cell stage Xenopus embryos differentiate into mesoderm when treated with activin A, whereas when cultured without activin they form atypical epidermis. The mesoderm tissue induced by activin is different between dorsal and ventral blastomeres. In the present study, the duration and timing of activin treatment was varied, in order to identify the critical stage when animal blastomeres acquire competence to respond to activin A. It was shown that the critical time was 45 min after blastomere isolation, which corresponds approximately to NF stage 6 (32-cell stage) of normal development. The competence gradually increased during the morula stages.
Language	English
Publishing date	2023-06-06
Publishing country	Japan
Document type	Journal Article
ZDB-ID	280433-5
ISSN	1440-169X ; 0012-1592
ISSN (online)	1440-169X
ISSN	0012-1592
DOI	10.1046/j.1440-169X.1995.t01-2-00008.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

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