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Article ; Online: Skewed distribution of spines is independent of presynaptic transmitter release and synaptic plasticity, and emerges early during adult neurogenesis.

Rößler, Nina / Jungenitz, Tassilo / Sigler, Albrecht / Bird, Alexander / Mittag, Martin / Rhee, Jeong Seop / Deller, Thomas / Cuntz, Hermann / Brose, Nils / Schwarzacher, Stephan W / Jedlicka, Peter

Open biology

2023 Volume 13, Issue 8, Page(s) 230063

Abstract: Dendritic spines are crucial for excitatory synaptic transmission as the size of a spine head correlates with the strength of its synapse. The distribution of spine head sizes follows a lognormal-like distribution with more small spines than large ones. ... ...

Abstract	Dendritic spines are crucial for excitatory synaptic transmission as the size of a spine head correlates with the strength of its synapse. The distribution of spine head sizes follows a lognormal-like distribution with more small spines than large ones. We analysed the impact of synaptic activity and plasticity on the spine size distribution in adult-born hippocampal granule cells from rats with induced homo- and heterosynaptic long-term plasticity
MeSH term(s)	Mice ; Rats ; Animals ; Neuronal Plasticity/physiology ; Neurons/physiology ; Pyramidal Cells/metabolism ; Dendritic Spines/metabolism ; Synaptic Transmission/physiology ; Synapses/physiology ; Neurogenesis
Language	English
Publishing date	2023-08-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2630944-0
ISSN	2046-2441 ; 2046-2441
ISSN (online)	2046-2441
ISSN	2046-2441
DOI	10.1098/rsob.230063
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Thesis: Untersuchung der synaptischen Neurotransmitterfreisetzung mit kombiniert elektrophysiologischen und bildgebenden Verfahren

Sigler, Albrecht

2005

Author's details	vorgelegt von Albrecht Sigler
Language	German
Size	VI, 120 S, Ill., graph. Darst
Document type	Book ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Göttingen, 2005
Note	Auch als elektronisches Dokument vorh. ; Zsfassung in engl. Sprache
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: In vivo 2-photon imaging of fine structure in the rodent brain: before, during, and after stroke.

Sigler, Albrecht / Murphy, Timothy H

Stroke

2010 Volume 41, Issue 10 Suppl, Page(s) S117–23

Abstract: The recent application of 2-photon microscopy to biological specimens has allowed investigators to examine individual synapses within live animals. The gain in resolution over conventional in vivo imaging techniques has been several orders of magnitude. ... ...

Abstract	The recent application of 2-photon microscopy to biological specimens has allowed investigators to examine individual synapses within live animals. The gain in resolution over conventional in vivo imaging techniques has been several orders of magnitude. We outline steps for the preparation and maintenance of animals for 2-photon microscopy of fine brain structure. We discuss the in vivo resolution of the method and the ability to image blood flow and synaptic structure in vivo. Applications of in vivo 2-photon microscopy include the study of synapse turnover in adult animals under normal conditions and during pathology such as stroke. In the case of stroke, 2-photon imaging has revealed marked swelling of dendrites and loss of spines within minutes of ischemic onset. Surprisingly, restoration of blood flow during reperfusion was associated with a return of relatively normal structure. Over longer time scales, 2-photon imaging revealed elevated rates of synaptogenesis within peri-infarct tissues recovering from stroke. These results provide an example of how high-resolution in vivo microscopy can be used to provide insight into both the acute pathology and recovery from stroke damage.
MeSH term(s)	Animals ; Brain/pathology ; Brain/physiopathology ; Brain Ischemia/pathology ; Brain Ischemia/physiopathology ; Dendritic Spines/pathology ; Mice ; Microscopy, Fluorescence, Multiphoton/methods ; Neurons/pathology ; Rats ; Stroke/pathology ; Stroke/physiopathology ; Synapses/pathology
Language	English
Publishing date	2010-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80381-9
ISSN	1524-4628 ; 0039-2499 ; 0749-7954
ISSN (online)	1524-4628
ISSN	0039-2499 ; 0749-7954
DOI	10.1161/STROKEAHA.110.594648
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Book ; Online ; Thesis: Untersuchung der synaptischen Neurotransmitterfreisetzung mit kombiniert elektrophysiologischen und bildgebenden Verfahren

Sigler, Albrecht

3260

2005

Abstract: Neuronale Netzwerke im Gehirn bestehen aus exzitatorischen und inhibitorischen Nervenzellen. Die exzitatorischen und inhibitorischen Neuronen spielen offensichtlich unterschiedliche Rollen im funktionalen Netzwerk, denn die von exzitatorischen und ... ...

Title variant	Investigation of the synaptic neurotransmitter release with combined electrophysiological and imaging techniques
Author's details	vorgelegt von Albrecht Sigler
Abstract	Neuronale Netzwerke im Gehirn bestehen aus exzitatorischen und inhibitorischen Nervenzellen. Die exzitatorischen und inhibitorischen Neuronen spielen offensichtlich unterschiedliche Rollen im funktionalen Netzwerk, denn die von exzitatorischen und inhibitorischen Synapsen freigesetzten Neurotransmitter haben unter nativen Bedingungen auf die empfangenen Neuronen einen entgegengesetzten Effekt. Es ist sehr naheliegend, daß Synapsen, die so unterschiedlich wirkende Neurotransmitter freisetzen, sich auch morphologisch und in der Funktionsweise des zugrundeliegenden Freisetzungsprozesses unterscheiden. Ziel der vorliegenden Arbeit war es, experimentell zu überprüfen, ob exzitatorische und inhibitorische Synapsen tatsächlich unterschiedliche funktionale Eigenschaften haben. Dazu wurden Neuronen aus primärer autaptischer Zellkultur von Gewebe aus dem Hippocampus beziehungsweise Striatum der Maus gewonnen ...
Language	German
Size	Online-Ressource, Ill., graph. Darst
Publisher	Niedersächsische Staats- und Universitätsbibliothek
Publishing place	Göttingen
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Univ., Diss.--Göttingen, 2005
Database	Former special subject collection: coastal and deep sea fishing

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Book ; Online ; Thesis: Untersuchung der synaptischen Neurotransmitterfreisetzung mit kombiniert elektrophysiologischen und bildgebenden Verfahren

Sigler, Albrecht [Verfasser]

2006

Author's details	vorgelegt von Albrecht Sigler
Keywords	Biowissenschaften, Biologie ; Life Science, Biology
Subject code	sg570
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Formation and Maintenance of Functional Spines in the Absence of Presynaptic Glutamate Release.

Sigler, Albrecht / Oh, Won Chan / Imig, Cordelia / Altas, Bekir / Kawabe, Hiroshi / Cooper, Benjamin H / Kwon, Hyung-Bae / Rhee, Jeong-Seop / Brose, Nils

Neuron

2017 Volume 94, Issue 2, Page(s) 304–311.e4

Abstract: Dendritic spines are the major transmitter reception compartments of glutamatergic synapses in most principal neurons of the mammalian brain and play a key role in the function of nerve cell circuits. The formation of functional spine synapses is thought ...

Abstract	Dendritic spines are the major transmitter reception compartments of glutamatergic synapses in most principal neurons of the mammalian brain and play a key role in the function of nerve cell circuits. The formation of functional spine synapses is thought to be critically dependent on presynaptic glutamatergic signaling. By analyzing CA1 pyramidal neurons in mutant hippocampal slice cultures that are essentially devoid of presynaptic transmitter release, we demonstrate that the formation and maintenance of dendrites and functional spines are independent of synaptic glutamate release.
MeSH term(s)	Animals ; Calcium/metabolism ; Dendrites/metabolism ; Dendritic Spines/metabolism ; Glutamic Acid/metabolism ; Hippocampus/metabolism ; Mice ; Signal Transduction/physiology ; Synapses/metabolism ; Synapses/physiology
Chemical Substances	Glutamic Acid (3KX376GY7L) ; Calcium (SY7Q814VUP)
Language	English
Publishing date	2017-04-19
Publishing country	United States
Document type	Journal Article
ZDB-ID	808167-0
ISSN	1097-4199 ; 0896-6273
ISSN (online)	1097-4199
ISSN	0896-6273
DOI	10.1016/j.neuron.2017.03.029
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 2496: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 92: Show issues

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Article ; Online: Simple and cost-effective hardware and software for functional brain mapping using intrinsic optical signal imaging.

Harrison, Thomas C / Sigler, Albrecht / Murphy, Timothy H

Journal of neuroscience methods

2009 Volume 182, Issue 2, Page(s) 211–218

Abstract: We describe a simple and low-cost system for intrinsic optical signal (IOS) imaging using stable LED light sources, basic microscopes, and commonly available CCD cameras. IOS imaging measures activity-dependent changes in the light reflectance of brain ... ...

Abstract	We describe a simple and low-cost system for intrinsic optical signal (IOS) imaging using stable LED light sources, basic microscopes, and commonly available CCD cameras. IOS imaging measures activity-dependent changes in the light reflectance of brain tissue, and can be performed with a minimum of specialized equipment. Our system uses LED ring lights that can be mounted on standard microscope objectives or video lenses to provide a homogeneous and stable light source, with less than 0.003% fluctuation across images averaged from 40 trials. We describe the equipment and surgical techniques necessary for both acute and chronic mouse preparations, and provide software that can create maps of sensory representations from images captured by inexpensive 8-bit cameras or by 12-bit cameras. The IOS imaging system can be adapted to commercial upright microscopes or custom macroscopes, eliminating the need for dedicated equipment or complex optical paths. This method can be combined with parallel high resolution imaging techniques such as two-photon microscopy.
MeSH term(s)	Animals ; Brain Mapping/instrumentation ; Cerebral Cortex/physiology ; Cost-Benefit Analysis ; Data Interpretation, Statistical ; Electronics ; Equipment Design ; Image Processing, Computer-Assisted/economics ; Image Processing, Computer-Assisted/instrumentation ; Light ; Mice ; Software ; Somatosensory Cortex/anatomy & histology
Language	English
Publishing date	2009-09-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	282721-9
ISSN	1872-678X ; 0165-0270
ISSN (online)	1872-678X
ISSN	0165-0270
DOI	10.1016/j.jneumeth.2009.06.021
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Zs.A 1486: Show issues

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Article ; Online: Imaging rapid redistribution of sensory-evoked depolarization through existing cortical pathways after targeted stroke in mice.

Sigler, Albrecht / Mohajerani, Majid H / Murphy, Timothy H

Proceedings of the National Academy of Sciences of the United States of America

2009 Volume 106, Issue 28, Page(s) 11759–11764

Abstract: Evidence suggests that recovery from stroke damage results from the production of new synaptic pathways within surviving brain regions over weeks. To address whether brain function might redistribute more rapidly through preexisting pathways, we examined ...

Abstract	Evidence suggests that recovery from stroke damage results from the production of new synaptic pathways within surviving brain regions over weeks. To address whether brain function might redistribute more rapidly through preexisting pathways, we examined patterns of sensory-evoked depolarization in mouse somatosensory cortex within hours after targeted stroke to a subset of the forelimb sensory map. Brain activity was mapped with voltage-sensitive dye imaging allowing millisecond time resolution over 9 mm(2) of brain. Before targeted stroke, we report rapid activation of the forelimb area within 10 ms of contralateral forelimb stimulation and more delayed activation of related areas of cortex such as the hindlimb sensory and motor cortices. After stroke to a subset of the forelimb somatosensory cortex map, function was lost in ischemic areas within the forelimb map center, but maintained in regions 200-500 microm blood flow deficits indicating the size of a perfused, but nonfunctional, penumbra. In many cases, stroke led to only partial loss of the forelimb map, indicating that a subset of a somatosensory domain can function on its own. Within the forelimb map spared by stroke, forelimb-stimulated responses became delayed in kinetics, and their center of activity shifted into adjacent hindlimb and posterior-lateral sensory areas. We conclude that the focus of forelimb-specific somatosensory cortex activity can be rapidly redistributed after ischemic damage. Given that redistribution occurs within an hour, the effect is likely to involve surviving accessory pathways and could potentially contribute to rapid behavioral compensation or direct future circuit rewiring.
MeSH term(s)	Afferent Pathways/physiology ; Animals ; Brain Mapping ; Electrophysiology ; Forelimb/innervation ; Mice ; Neuronal Plasticity/physiology ; Somatosensory Cortex/metabolism ; Somatosensory Cortex/physiology ; Stroke/metabolism ; Stroke/physiopathology ; Synaptic Transmission/physiology
Language	English
Publishing date	2009-07-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.0812695106
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1148: Show issues

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Article ; Online: Automated and quantitative image analysis of ischemic dendritic blebbing using in vivo 2-photon microscopy data.

Chen, Shangbin / Tran, Sherri / Sigler, Albrecht / Murphy, Timothy H

Journal of neuroscience methods

2011 Volume 195, Issue 2, Page(s) 222–231

Abstract: Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled ...

Abstract	Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled with GFP/YFP under normal conditions and after ischemia-induced damage. In vivo 2-photon data from mouse layer 5 neurons with apical dendritic tufts extending to the cortical surface were examined before, during, and after global ischemia. To quantify changes in dendritic structure, we used morphometric tools that exploit characteristic features of blebbing, distinguished as localized regions of spherical or ellipsoid swellings. By comparing acquired images during ischemia and reperfusion to a pre-ischemia reference image, our automated approach detected blebs based on defined eccentricity and area thresholds and quantified the percentage of blebbed dendrites based on a block-selection method. Our results indicate that the automated morphometric indices we employ yield results that correlate with manual assessment. The automated approach permits rapid and effective analysis of dendritic structure and may facilitate the study of ischemic dendritic damage.
MeSH term(s)	Animals ; Automatic Data Processing/methods ; Dendrites/metabolism ; Dendrites/pathology ; Dendrites/ultrastructure ; Green Fluorescent Proteins/genetics ; Imaging, Three-Dimensional/methods ; Ischemia/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Photons ; Reproducibility of Results ; Somatosensory Cortex/metabolism ; Somatosensory Cortex/pathology ; Statistics as Topic ; Time Factors ; User-Computer Interface
Chemical Substances	Green Fluorescent Proteins (147336-22-9)
Language	English
Publishing date	2011-02-15
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	282721-9
ISSN	1872-678X ; 0165-0270
ISSN (online)	1872-678X
ISSN	0165-0270
DOI	10.1016/j.jneumeth.2010.12.018
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Zs.A 1486: Show issues

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Article: Imaging rapid redistribution of sensory-evoked depolarization through existing cortical pathways after targeted stroke in mice

Sigler, Albrecht / Mohajerani, Majid H / Murphy, Timothy H

Proceedings of the National Academy of Sciences of the United States of America. 2009 July 14, v. 106, no. 28

2009

Abstract	Evidence suggests that recovery from stroke damage results from the production of new synaptic pathways within surviving brain regions over weeks. To address whether brain function might redistribute more rapidly through preexisting pathways, we examined patterns of sensory-evoked depolarization in mouse somatosensory cortex within hours after targeted stroke to a subset of the forelimb sensory map. Brain activity was mapped with voltage-sensitive dye imaging allowing millisecond time resolution over 9 mmÂ² of brain. Before targeted stroke, we report rapid activation of the forelimb area within 10 ms of contralateral forelimb stimulation and more delayed activation of related areas of cortex such as the hindlimb sensory and motor cortices. After stroke to a subset of the forelimb somatosensory cortex map, function was lost in ischemic areas within the forelimb map center, but maintained in regions 200-500 Î¼m from blood flow deficits indicating the size of a perfused, but nonfunctional, penumbra. In many cases, stroke led to only partial loss of the forelimb map, indicating that a subset of a somatosensory domain can function on its own. Within the forelimb map spared by stroke, forelimb-stimulated responses became delayed in kinetics, and their center of activity shifted into adjacent hindlimb and posterior-lateral sensory areas. We conclude that the focus of forelimb-specific somatosensory cortex activity can be rapidly redistributed after ischemic damage. Given that redistribution occurs within an hour, the effect is likely to involve surviving accessory pathways and could potentially contribute to rapid behavioral compensation or direct future circuit rewiring.
Keywords	blood flow ; brain ; cortex ; image analysis ; mice ; stroke
Language	English
Dates of publication	2009-0714
Size	p. 11759-11764.
Publishing place	National Academy of Sciences
Document type	Article
ZDB-ID	209104-5
ISSN	1091-6490 ; 0027-8424
ISSN (online)	1091-6490
ISSN	0027-8424
DOI	10.1073/pnas.0812695106
Database	NAL-Catalogue (AGRICOLA)

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