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Article ; Online: Properties of bacterial cellulose acetate nanocomposite with TiO

Suryanto, Heru / Kurniawan, Fredy / Syukri, Daimon / Binoj, Joseph Selvi / Hari, Purnama Dini / Yanuhar, Uun

International journal of biological macromolecules

2023 Volume 235, Page(s) 123705

Abstract: Agricultural waste is considered a promising source for bacterial cellulose production. This study aims to observe the influence of ... ...

Abstract	Agricultural waste is considered a promising source for bacterial cellulose production. This study aims to observe the influence of TiO
MeSH term(s)	Graphite/chemistry ; Cellulose/chemistry ; Nanocomposites/chemistry ; Nanoparticles/chemistry ; Bacteria
Chemical Substances	acetylcellulose (3J2P07GVB6) ; titanium dioxide (15FIX9V2JP) ; Graphite (7782-42-5) ; Cellulose (9004-34-6)
Language	English
Publishing date	2023-02-16
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.123705
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Article ; Online: Properties of bacterial cellulose acetate nanocomposite with TiO2 nanoparticle and graphene reinforcement

Suryanto, Heru / Kurniawan, Fredy / Syukri, Daimon / Binoj, Joseph Selvi / Hari, Purnama Dini / Yanuhar, Uun

International Journal of Biological Macromolecules. 2023 Apr., v. 235 p.123705-

2023

Abstract: Agricultural waste is considered a promising source for bacterial cellulose production. This study aims to observe the influence of TiO₂ nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for ... ...

Abstract	Agricultural waste is considered a promising source for bacterial cellulose production. This study aims to observe the influence of TiO₂ nanoparticles and graphene on the characteristic of bacterial cellulose acetate-based nanocomposite membranes for bacterial filtration in waters. Bacterial cellulose was produced from the pineapple peel waste using fermentation process. High-pressure homogenization process was applied to reduce bacterial nanocellulose size and esterification process was carried out to produce cellulose acetate. Nanocomposite membranes were synthesized with reinforcement of TiO₂ nanoparticles 1 % and graphene nanopowder 1 %. The nanocomposite membrane was characterized using an FTIR, SEM, XRD, BET, tensile testing, and bacterial filtration effectiveness using the plate count method. The results showed that the main cellulose structure was identified at the diffraction angle 22° and the cellulose structure slightly changed at the peak of diffraction angles of 14° and 16°. In addition, the crystallinity of bacterial cellulose increased from 72.5 % to 75.9 %, and the functional group analysis showed that several peak shifts indicated a change in the functional group of membrane. Similarly, the surface morphology of membrane became rougher with the structure of mesoporous membrane. Moreover, adding TiO₂ and graphene increases crystallinity and bacterial filtration effectiveness of nanocomposite membrane.
Keywords	agricultural wastes ; cellulose ; cellulose acetate ; crystal structure ; esterification ; fermentation ; filtration ; graphene ; homogenization ; nanocomposites ; nanoparticles ; pineapple peels ; plate count ; porous media ; titanium dioxide ; Nanocomposite
Language	English
Dates of publication	2023-04
Publishing place	Elsevier B.V.
Document type	Article ; Online
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.123705
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Article: In vivo

Yanuhar, Uun / Nurcahyo, Hendra / Widiyanti, Luluk / Junirahma, Nur Sakinah / Caesar, Nico Rahman / Sukoso, Sukoso

Veterinary world

2022 Volume 15, Issue 5, Page(s) 1269–1282

Abstract: Background and aim: The need for fish seeds resistant to bacterial and viral infections has encouraged studies on the molecular pathogenesis mechanism of : Materials and methods: The study was conducted by isolating crude protein and whole cells from ...

Abstract	Background and aim: The need for fish seeds resistant to bacterial and viral infections has encouraged studies on the molecular pathogenesis mechanism of Materials and methods: The study was conducted by isolating crude protein and whole cells from both the Results: These results indicated that all the specific receptors for protein in fish organs recognized vibriosis antigens. The specificity test showed that the brain, eye, and kidney organs' receptors provided a quality and quantity level of responses at 22.63, 53.95, and 43.15 kDa, respectively. The polyclonal anti- Conclusion: In the future, the molecular characteristics of
Language	English
Publishing date	2022-05-23
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2022.1269-1282
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Article: Immunomolecular response of CD4+, CD8+, TNF-α and IFN-γ in Myxobolus-infected koi (Cyprinus carpio) treated with probiotics

Yanuhar, Uun / Caesar, Nico Rahman / Junirahma, Nur Sakinah / Soelistyoadi, Rachmat Noer

Shanghai Ocean University. Aquaculture and fisheries. 2022 Jan. 05,

2022

Abstract: A poor environment increases fish's susceptibility to myxosporean infection that can cause the death of larval fish, especially for koi fish (Cyprinus carpio). This study aimed to determine the effect of probiotics, local anti-parasitic drugs (kutuklin), ...

Abstract	A poor environment increases fish's susceptibility to myxosporean infection that can cause the death of larval fish, especially for koi fish (Cyprinus carpio). This study aimed to determine the effect of probiotics, local anti-parasitic drugs (kutuklin), and the chemical compound diflubenzuron treatments on the koi immune response. This study used PCR with specific primer 18S SSU rDNA and DNA sequencing to detect Myxobulus phylogenetic. The treatments were divided into 5 groups: Treatment (A) (healthy koi without treatment), (B) (infected koi without treatment), (C) (infected koi with 0.55 mL/30 L probiotics), (D) (infected koi with 1 μL/g of feed kutuklin), and (E) (infected koi with 0.02 mg/5 L dimilin). Myxospore has observed with Scanning Electron Microscopy (SEM) and 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. The histological analysis using semi-quantitative scoring methods, and flow cytometry was conducted to analyse the immune response of Cluster of differentiation 4 (CD4⁺), Cluster of differentiation 8 (CD8⁺), Tumor Necrosis Factor-alpha (TNF-α), Interferon gamma (IFN-γ) cells in the gills. Results show that the histological analysis indicated edema, hyperplasia, lamella fusion, congestion, and hypertrophy lesions in infected koi. Treatment with probiotics shows the lowest damage (30.6%). The immune responses of CD4⁺ and CD8⁺ cells to dimilin treatment were 10.54% and 16.86%, respectively. The largest TNF-α and IFN-γ response were for the kutuklin treatment (29.26%) and probiotics treatment (8.23%).
Keywords	Cyprinus carpio ; aquaculture ; death ; diflubenzuron ; edema ; fish larvae ; flow cytometry ; fluorescence ; histology ; hyperplasia ; hypertrophy ; immune response ; interferon-gamma ; koi ; phylogeny ; probiotics ; tumor necrosis factor-alpha
Language	English
Dates of publication	2022-0105
Publishing place	Elsevier B.V.
Document type	Article
Note	Pre-press version
ISSN	2468-550X
DOI	10.1016/j.aaf.2022.01.004
Database	NAL-Catalogue (AGRICOLA)

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Article: Evaluation of phytochemical and superoxide dismutase activities of

Menajang, Febry S I / Mahmudi, Mohammad / Yanuhar, Uun / Herawati, Endang Yuli

Veterinary world

2020 Volume 13, Issue 4, Page(s) 676–680

Abstract: Background and aim: Seagrasses are an excellent and potential bioresource to discover new natural bioactive compounds such as antioxidants that have beneficial effects on health. Natural antioxidants have many functions in biological systems, primarily ... ...

Abstract	Background and aim: Seagrasses are an excellent and potential bioresource to discover new natural bioactive compounds such as antioxidants that have beneficial effects on health. Natural antioxidants have many functions in biological systems, primarily for defense against oxidation which produces free radicals in food, chemicals, and living systems. This study aimed to discover new natural antioxidant agents, Materials and methods: Sample specimens of Results: Extracts of the seagrass Conclusion: E. acoroides
Language	English
Publishing date	2020-04-13
Publishing country	India
Document type	Journal Article
ZDB-ID	2456277-4
ISSN	2231-0916 ; 0972-8988
ISSN (online)	2231-0916
ISSN	0972-8988
DOI	10.14202/vetworld.2020.676-680
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Article ; Online: In vivo test of Vibrio alginolyticus and Vibrio harveyi infection in the humpback grouper (Cromileptes altivelis) from East Java Indonesia

Uun Yanuhar / Hendra Nurcahyo / Luluk Widiyanti / Nur Sakinah Junirahma / Nico Rahman Caesar / Sukoso Sukoso

Veterinary World, Vol 15, Iss 5, Pp 1269-

2022 Volume 1282

Abstract: Background and Aim: The need for fish seeds resistant to bacterial and viral infections has encouraged studies on the molecular pathogenesis mechanism of Vibrio bacteria, such as Vibrio alginolyticus and Vibrio harveyi, regarding the receptor organs, ... ...

Abstract	Background and Aim: The need for fish seeds resistant to bacterial and viral infections has encouraged studies on the molecular pathogenesis mechanism of Vibrio bacteria, such as Vibrio alginolyticus and Vibrio harveyi, regarding the receptor organs, protein adhesion mechanisms, and antibody responses of the humpback grouper. This study aims to confirm the characteristics of the specific proteins expressed in the receptor organ of the humpback grouper (Cromileptes altivelis) using the expression of V. alginolyticus and V. harveyi bacteria. Materials and Methods: The study was conducted by isolating crude protein and whole cells from both the Vibrio bacteria. In addition, serum and organ tissue were also isolated from fish samples. Then, hemagglutination and dot blot tests with polyacrylamide gel electrophoresis analysis were performed to determine the highest expression of receptor from the whole bacterial cells and crude protein from both healthy and infected (V. alginolyticus and V. harveyi) fishes. Scanning electron microscope results showed that V. alginolyticus and V. harveyi could express bundle-forming pili, which is involved in bacterial autoaggregation and the mediation of the initial attachment of bacteria to their host cells. Results: These results indicated that all the specific receptors for protein in fish organs recognized vibriosis antigens. The specificity test showed that the brain, eye, and kidney organs' receptors provided a quality and quantity level of responses at 22.63, 53.95, and 43.15 kDa, respectively. The polyclonal anti-V. alginolyticus immunoglobulin M (IgM) antibodies were more cross-reactive than the anti-V. harveyi IgM. Hence, this shows that V. alginolyticus bacteria are more pathogenic than V. harveyi. Conclusion: In the future, the molecular characteristics of V. alginolyticus and V. harveyi antigens and the specific receptor organ proteins in the humpback grouper can be developed as the basis for constructing molecular peptide-based vaccine materials.
Keywords	crude protein ; humpback grouper ; vibrio alginolyticus ; vibrio harveyi ; western blot ; Animal culture ; SF1-1100 ; Veterinary medicine ; SF600-1100
Subject code	616
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Veterinary World
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Titanium Dioxide Nanoparticle on Properties of Nanocomposite Membrane Made of Bacterial Cellulose

Yanuhar, Uun / Suryanto, Heru / Sardjono, Susanto Arif / Ningrum, Irma K. / Aminnudin, Aminnudin / Binoj, J S

Journal of Natural Fibers. 2022 Nov. 28, v. 19, no. 16 p.13914-13927

2022

Abstract: The purpose of this study was to see the effect of adding TiO₂ nanoparticles had on the characteristics of bacterial nanocellulose (BNC)-based membranes. Pellicles were created from the pineapple peel waste extract, and to obtain BNC, pellicle was ... ...

Abstract	The purpose of this study was to see the effect of adding TiO₂ nanoparticles had on the characteristics of bacterial nanocellulose (BNC)-based membranes. Pellicles were created from the pineapple peel waste extract, and to obtain BNC, pellicle was disintegrated in six cycles using high-pressure homogenizer at 150 bar. The concentrations of TiO₂ nanoparticles in the membrane reinforcement solution were 0.5, 1.0, 1.5, 2.0, and 2.5 wt.%. They were sonicated and dried in an oven at 60°C for 8 h. X-ray diffraction, Fourier transform infrared spectroscopy, roughness test, mechanical properties, and scanning electron microscopy were investigated to explore its claims. The results revealed that diffraction pattern of BNC/TiO₂ membrane has a new peak at 25.05° that ensures the presence of TiO₂ nanoparticles as well as diffraction peak occurred at 22.49°, which indicates the presence of cellulose Iβ. Moreover, the addition of TiO₂ nanoparticles until 1.5 wt.%, the crystallinity index is relatively same and the optimum strength of 99.5 MPa was attained. Further, improvement in addition of TiO₂ nanoparticle at 2 wt.% showed a decline in crystallinity index as 80.16% to 74.72% at 2.5 wt.%. The morphology of the membrane surface revealed that TiO₂ nanoparticles were still agglomerated and not equally distributed across the BNC membrane, leading to increase in surface roughness.
Keywords	Fourier transform infrared spectroscopy ; X-ray diffraction ; cellulose ; crystal structure ; electron microscopy ; homogenizers ; nanocomposites ; nanoparticles ; ovens ; pineapple peels ; roughness ; surface roughness ; titanium dioxide ; Bacterial cellulose ; membrane ; crystalline index ; nanocomposite ; pineapple waste
Language	English
Dates of publication	2022-1128
Size	p. 13914-13927.
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	2182715-1
ISSN	1544-046X ; 1544-0478
ISSN (online)	1544-046X
ISSN	1544-0478
DOI	10.1080/15440478.2022.2112797
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Analysis of The Secondary Metabolite of Kersen Leaf Extracts (Muntingia calabura L.) and Its Potential as Anti-Bacteria to Inhibit Aeromonas hydrophila

Gede Angga Krishna Fariestha / Sri Andayani / Uun Yanuhar

Research Journal of Life Science, Vol 5, Iss 2, Pp 121-

2018 Volume 127

Abstract: Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, ... ...

Abstract	Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic anti-bacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.
Keywords	Anti-bacterial ; Muntingia calabura L ; Aeromonas hydrophila ; Secondary metabolite ; Agriculture (General) ; S1-972 ; Medicine (General) ; R5-920
Subject code	500
Language	English
Publishing date	2018-11-01T00:00:00Z
Publisher	University of Brawijaya
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Screening of NO Inhibitor Release Activity from Soft Coral Extracts Origin Palu Bay, Central Sulawesi, Indonesia.

Tanod, Wendy Alexander / Yanuhar, Uun / Maftuch / Putra, Masteria Yunovilsa / Risjani, Yenny

Anti-inflammatory & anti-allergy agents in medicinal chemistry

2019 Volume 18, Issue 2, Page(s) 126–141

Abstract: Background: As a marine organism, soft corals can be utilized to be various bioactive substances, especially terpenoids and steroids. The soft corals family which produces bioactive generally come from clavulariidae, alcyoniidae, nephtheidae and ... ...

Abstract	Background: As a marine organism, soft corals can be utilized to be various bioactive substances, especially terpenoids and steroids. The soft corals family which produces bioactive generally come from clavulariidae, alcyoniidae, nephtheidae and xeniidae family. Objective: To investigate the bioactivity of Nitric Oxide (NO) inhibitor release from soft coral crude extracts of Sinularia sp. (SCA), Nephthea sp. (SCB), Sarcophyton sp. (SCC), Sarcophyton sp. (SCD), Sinularia sp. (SCE) and Sinularia sp. (SCF). Materials and methods: Soft coral is collected from Palu Bay (Central Sulawesi). NO inhibitory release activity measured according to the Griess reaction. Soft corals sample macerated with 1:2 (w/v). Then, Soft coral extracts with the best NO Inhibitor activity partitioned with Dichloromethane, Ethyl acetate, and n-butanol. The bioactive of all crude extracts were identified by GC-MS to find compounds with anti-inflammatory potential. Results: Sarcophyton sp. (SCC) and Sinularia sp. (SCF) are able to inhibit NO concentrations of 0.22 ± 0.04 and 0.20 ± 0.04 µM at 20 mg/mL, respectively. The chemical constituents determined and showed the potential as anti-inflammatory in the crude of Sinularia sp. (SCA) were Octacosane (3.25%). In Nephthea sp., (SCB) were Cyclohexene, 6-ethenyl-6- methyl-1-(1-methylethyl)-3-(1-methylethylidene)-,(S)- (0.55%); Azulene, 1,2,3,4,5,6,7,8- octahydro-1,4-dimethyl-7-(1-methylethylidene)-, (1S-cis)- (0.53%); and 1,7,7-Trimethyl- 2-vinylbicyclo[2.2.1]hept-2-ene (4.72%). In Sarcophyton sp, (SCC) were Eicosane (0.12%); Nonacosane (10.7%); 14(β)-Pregnane (0.87%); Octacosane 6.39%); and Tricosane (1.53%). In Sarcophyton sp. (SCD) were 14(β)-Pregnane (2.69%); and Octadecane (27.43%). In crude of Sinularia sp. (SCE) were Oleic Acid (0.63%); 7,10-Hexadecadienoic acid, methyl ester (0.54%); 14(β)-Pregnane (1.07%); 5,8,11,14-Eicosatetraenoic acid, ethyl ester, (all-Z)- (4.60%); Octacosane (7.75%); and 1,2-Benzisothiazole, 3-(hexahydro-1Hazepin- 1-yl)-, 1,1-dioxide (1.23%). In the crude of Sinularia sp., (SCF) were Oxirane, decyl- (1.38%); Nonacosane (0.57%); Cyclohexanol, 5-methyl-2-(1-methylethenyl)- (0.61%); 14B-Pregnane (0.76%); and Tetratriacontane (1.02%). Conclusion: The extract of Sarcophyton sp. (SCC) and Sinularia sp. (SCF) showed the best NO inhibitory release activity. This study is making soft corals from Central Sulawesi, Indonesia can become a potential organism in the discovery and development of bioactive substances anti-inflammatory.
MeSH term(s)	Alkanes ; Animals ; Anthozoa/chemistry ; Anthozoa/immunology ; Anti-Inflammatory Agents/pharmacology ; Biological Therapy/trends ; Cell Extracts ; Cyclohexenes ; Drug Evaluation, Preclinical ; Free Radical Scavengers/chemistry ; Free Radical Scavengers/pharmacology ; Indonesia ; Nitric Oxide/metabolism
Chemical Substances	Alkanes ; Anti-Inflammatory Agents ; Cell Extracts ; Cyclohexenes ; Free Radical Scavengers ; cyclohexene (12L0P8F7GN) ; Nitric Oxide (31C4KY9ESH) ; eicosane (3AYA9KEC48) ; octacosane (VFF49836P8)
Language	English
Publishing date	2019-02-08
Publishing country	United Arab Emirates
Document type	Journal Article
ZDB-ID	2227189-2
ISSN	1875-614X ; 1871-5230
ISSN (online)	1875-614X
ISSN	1871-5230
DOI	10.2174/1871523018666190222115034
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Article ; Online: Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus

BUDI RIANTO WAHIDI / UUN YANUHAR / MOHAMAD MOHAMAD FADJAR / SRI ANDAYANI

Biodiversitas, Vol 20, Iss 2, Pp 497-

2019 Volume 503

Abstract: Abstract. Wahidi BR, Yanuhar U, Fadjar M, Andayani A. 2019. Pathognomonic features and ...

Abstract	Abstract. Wahidi BR, Yanuhar U, Fadjar M, Andayani A. 2019. Pathognomonic features and ultrastructural of Koi Herpesvirus infected Oreochromis niloticus. Biodiversitas 20: 497-503. Koi Herpesvirus (KHV) has caused considerable losses in the cultivation of the Cyprinidae family worldwide. In addition to infecting Cyprinidae, KHV can also infect other freshwater fish. This study reports histopathology and ultrastructural analysis of KHV that infect Nile tilapia. Although there are no specific clinical symptoms, pathognomonic indicating Nile tilapia fish has been infected with KHV have been found, i.e. through the formation of intranuclear inclusion body and cell swelling which essentially experience chromatin margination. In addition, histopathology results indicate changes or damage to other tilapia tissue, i.e. necrosis, hypertrophy, edema, hemorrhage, inflammation, congestion, degeneration, and apoptosis. These results indicate that KHV has been distributed and replicated in tilapia organs. Based on the Transmission Electron Microscopy (TEM) analysis, KHV virions have been detected in the cytoplasm or outside the cells on a size of 150-170 nm.
Keywords	Biology (General) ; QH301-705.5
Subject code	621
Language	English
Publishing date	2019-02-01T00:00:00Z
Publisher	MBI & UNS Solo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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