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  1. Article ; Online: Assessment of antibody kinetics in mpox cases with indigenously developed IgM and IgG ELISA.

    Shete, Anita M / Patil, Deepak Y / Jain, Rajlaxmi / Sahay, Rima R / Chenayil, Shubin / Mohandas, Sreelekshmy / Yadav, Pragya D

    Journal of medical virology

    2023  Volume 95, Issue 9, Page(s) e29073

    MeSH term(s) Humans ; Kinetics ; Mpox (monkeypox) ; Enzyme-Linked Immunosorbent Assay ; Immunoglobulin G ; Immunoglobulin M
    Chemical Substances Immunoglobulin G ; Immunoglobulin M
    Language English
    Publishing date 2023-09-06
    Publishing country United States
    Document type Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.29073
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  2. Article ; Online: A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples

    Anita Shete / Sreelekshmy Mohandas / Rajlaxmi Jain / Pragya D. Yadav

    STAR Protocols, Vol 2, Iss 2, Pp 100573- (2021)

    2021  

    Abstract: Summary: This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation ... ...

    Abstract Summary: This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigens and the negative control antigen and the use of antigen-coated microtiter plates to detect SARS-CoV-2-specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive versus negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently prepared antigens.For complete details on the use and execution of this protocol, please refer to Mohandas et al. (2021).
    Keywords Immunology ; Microbiology ; Model Organisms ; Antibody ; Science (General) ; Q1-390
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
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  3. Article ; Online: A qualitative IgG ELISA for detection of SARS-CoV-2-specific antibodies in Syrian hamster serum samples.

    Shete, Anita / Mohandas, Sreelekshmy / Jain, Rajlaxmi / Yadav, Pragya D

    STAR protocols

    2021  Volume 2, Issue 2, Page(s) 100573

    Abstract: This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of ... ...

    Abstract This protocol describes an indirect enzyme-linked immunosorbent assay for qualitative detection of IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Syrian hamster serum samples. We describe the preparation of inactivated virus antigens and the negative control antigen and the use of antigen-coated microtiter plates to detect SARS-CoV-2-specific antibodies from SARS-CoV-2-infected hamsters, including the criteria for differentiating positive versus negative reaction. The limited batch-to-batch variability of this assay has been verified with two batches of independently prepared antigens. For complete details on the use and execution of this protocol, please refer to Mohandas et al. (2021).
    MeSH term(s) Animals ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/immunology ; COVID-19/virology ; Coronavirus Nucleocapsid Proteins/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Immunologic Tests/methods ; Mesocricetus ; SARS-CoV-2/immunology
    Chemical Substances Antibodies, Viral ; Coronavirus Nucleocapsid Proteins ; Immunoglobulin G
    Language English
    Publishing date 2021-05-10
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2021.100573
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  4. Article: Antibody response in symptomatic & asymptomatic Nipah virus cases from Kerala, India.

    Shete, Anita M / Radhakrishnan, Chandni / Pardeshi, Prachi G / Yadav, Pragya D / Jain, Rajlaxmi / Sahay, Rima R / Sugunan, A P

    The Indian journal of medical research

    2022  Volume 154, Issue 3, Page(s) 533–535

    MeSH term(s) Antibody Formation ; Disease Outbreaks ; Henipavirus Infections/epidemiology ; Humans ; India/epidemiology ; Nipah Virus
    Language English
    Publishing date 2022-02-10
    Publishing country India
    Document type Journal Article
    ZDB-ID 390883-5
    ISSN 0971-5916 ; 0019-5340
    ISSN 0971-5916 ; 0019-5340
    DOI 10.4103/ijmr.IJMR_4388_20
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  5. Article ; Online: A case of human buffalopox in Malappuram, India: The role of mpox surveillance in 2022.

    Patil, Deepak Y / George, Sandhya / Sahay, Rima R / Chenayil, Shubin / Shete, Anita M / Bhaskaramenon, Sindhu C / Anish, Thekkumkara Surendran / Jain, Rajlaxmi / Nazeer, Fathima Abdul / John, Reena / Joshi, Yash / Sivaprasad, R / Waghmare, Priyanka / Ray, Shilpa / Balakrishnan, Swapna / Kumari, Vaishnavi / Sathe, Shubhangi / Yadav, Pragya D

    Journal of medical virology

    2023  Volume 95, Issue 2, Page(s) e28580

    MeSH term(s) Humans ; Mpox (monkeypox) ; Vaccinia virus ; Vaccinia ; India/epidemiology
    Language English
    Publishing date 2023-03-02
    Publishing country United States
    Document type Case Reports ; Letter ; Research Support, Non-U.S. Gov't
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.28580
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  6. Article ; Online: Detection of possible Nipah virus infection in Rousettus leschenaultii and Pipistrellus Pipistrellus bats in Maharashtra, India.

    Gokhale, Mangesh D / Sreelekshmy, Mohandas / Sudeep, Anakkatil B / Shete, Anita / Jain, Rajlaxmi / Yadav, Pragya D / Mathapati, Basavraj / Mourya, Devendra T

    Journal of infection and public health

    2021  Volume 14, Issue 8, Page(s) 1010–1012

    MeSH term(s) Animals ; Chiroptera ; Henipavirus Infections/epidemiology ; Henipavirus Infections/veterinary ; Humans ; India/epidemiology
    Language English
    Publishing date 2021-06-11
    Publishing country England
    Document type Editorial
    ZDB-ID 2467587-8
    ISSN 1876-035X ; 1876-0341
    ISSN (online) 1876-035X
    ISSN 1876-0341
    DOI 10.1016/j.jiph.2021.05.001
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  7. Article ; Online: Clinical, epidemiological, and molecular investigation of Kyasanur forest disease from Karnataka state, India during 2018-2019.

    Munivenkatappa, Ashok / Yadav, Pragya D / Sahay, Rima R / Sk, Kiran / Shete, Anita M / Patil, Deepak Y / Mohandas, Sreelekshmy / Jain, Rajlaxmi / Patil, Savita / Sinha, Diamond P / Jayaswamy, Manjunatha M

    Infectious diseases (London, England)

    2023  Volume 56, Issue 2, Page(s) 145–156

    Abstract: Background: In this study, we carried out an investigation of Kyasanur Forest Disease (KFD) suspected human cases reported in Karnataka state, India from December 2018 to June 2019.: Methods: The clinical samples of KFD suspected cases (: Results: ...

    Abstract Background: In this study, we carried out an investigation of Kyasanur Forest Disease (KFD) suspected human cases reported in Karnataka state, India from December 2018 to June 2019.
    Methods: The clinical samples of KFD suspected cases (
    Results: Of all, 173 (8.8%) cases tested positive for KFD either by real-time RT-PCR (
    Conclusion: Although the KFD has been endemic in many districts of Karnataka state, our study confirms the presence of KFDV for the first time in two new districts, i.e. Hassan and Mysore. A comparative analysis of KFDV infection among the KFD-vaccinated and non-vaccinated populations demonstrated an insignificant difference.
    MeSH term(s) Animals ; Humans ; Kyasanur Forest Disease/epidemiology ; Kyasanur Forest Disease/diagnosis ; Chikungunya Fever/epidemiology ; India/epidemiology ; Immunoglobulin M ; Haplorhini ; Dengue/epidemiology
    Chemical Substances Immunoglobulin M
    Language English
    Publishing date 2023-12-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2839775-7
    ISSN 2374-4243 ; 2374-4235
    ISSN (online) 2374-4243
    ISSN 2374-4235
    DOI 10.1080/23744235.2023.2282042
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  8. Article ; Online: Detection of possible Nipah virus infection in Rousettus leschenaultii and Pipistrellus Pipistrellus bats in Maharashtra, India

    Mangesh .D. Gokhale / Mohandas Sreelekshmy / Anakkatil B. Sudeep / Anita Shete / Rajlaxmi Jain / Pragya D. Yadav / Basavraj Mathapati / Devendra T. Mourya

    Journal of Infection and Public Health, Vol 14, Iss 8, Pp 1010-

    2021  Volume 1012

    Keywords Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
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  9. Article ; Online: Antibody response in symptomatic & asymptomatic Nipah virus cases from Kerala, India

    Anita M Shete / Chandni Radhakrishnan / Prachi G Pardeshi / Pragya D Yadav / Rajlaxmi Jain / Rima R Sahay / A P Sugunan

    Indian Journal of Medical Research, Vol 154, Iss 3, Pp 533-

    2021  Volume 535

    Keywords Medicine ; R
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher Wolters Kluwer Medknow Publications
    Document type Article ; Online
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  10. Article ; Online: Development and evaluation of indirect antibody ELISA assay for early diagnosis and surveillance of Crimean-Congo hemorrhagic fever infection in humans.

    Shrivastava, Neha / Kumar, Jyoti S / Yadav, Pragya / Sharma, Shashi / Shete, Anita M / Jain, Rajlaxmi / Shrivastava, Ambuj / Dash, Paban Kumar

    Virus research

    2022  Volume 313, Page(s) 198717

    Abstract: Crimean-Congo hemorrhagic fever (CCHF) is an important tick borne zoonotic viral disease of humans. CCHF virus causes sporadic cases of severe illness across a huge geographic area across Africa to Europe to Asia including India. CCHF has emerged as a ... ...

    Abstract Crimean-Congo hemorrhagic fever (CCHF) is an important tick borne zoonotic viral disease of humans. CCHF virus causes sporadic cases of severe illness across a huge geographic area across Africa to Europe to Asia including India. CCHF has emerged as a major public health concern in western Indian states including Gujarat and Rajasthan, where regular human cases were reported since the year 2011. Human serve as the dead-end host, and they gain infection via infected tick bite, in close contact with ruminants and from slaughter house. Currently, the detection of this fatal infection is limited to BSL-4 laboratory which is scarce even in developed economies. Thus, a safe, sensitive assay for early immunodiagnosis is crucial for disease management and containing the outbreak. In this study, the conserved recombinant nucleoprotein was exploited as a safe, scalable alternate antigen for development of indirect IgM and IgG ELISA detection platform. The indirect ELISA was evaluated using suspected clinical samples collected from hotspot areas in India. Comparison with reference MAC ELISA and IgG ELISA revealed a correlation of 95% and 100% respectively. The results indicate that the developed IgM and IgG indirect ELISA has high sensitivity and specificity for detecting CCHFV antibodies among human. These assays are easy to perform and can be employed for high throughput screening of human samples for clinical diagnosis as well as serosurveillance. These assays are also amenable for conversion to low-cost point of care testing formats for application in resource limited settings.
    MeSH term(s) Animals ; Antibodies, Viral ; Early Diagnosis ; Enzyme-Linked Immunosorbent Assay/methods ; Hemorrhagic Fever Virus, Crimean-Congo ; Hemorrhagic Fever, Crimean/diagnosis ; Hemorrhagic Fever, Crimean/epidemiology ; Humans ; India/epidemiology
    Chemical Substances Antibodies, Viral
    Language English
    Publishing date 2022-02-18
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2022.198717
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