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Article: Qian-Zheng-San

Wang, Zhi-Yong / Qin, Li-Hua / Zhang, Wei-Guang / Zhang, Pei-Xun / Jiang, Bao-Guo

2019 Volume 14, Issue 4, Page(s) 683–691

Abstract: Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx ... However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury ... by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian ...

Abstract	Qian-Zheng-San, a traditional Chinese prescription consisting of Typhonii Rhizoma, Bombyx Batryticatus, Scorpio, has been found to play an active therapeutic role in central nervous system diseases. However, it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury. Therefore, we used Sprague-Dawley rats to investigate this. A sciatic nerve crush injury model was induced by clamping the right sciatic nerve. Subsequently, rats in the treatment group were administered 2 mL Qian-Zheng-San (1.75 g/mL) daily as systemic therapy for 1, 2, 4, or 8 weeks. Rats in the control group were not administered Qian-Zheng-San. Rats in sham group did not undergo surgery and systemic therapy. Footprint analysis was used to assess nerve motor function. Electrophysiological experiments were used to detect nerve conduction function. Immunofluorescence staining was used to assess axon counts and morphological analysis. Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord. At 2 and 4 weeks postoperatively, the sciatic nerve function index, nerve conduction velocity, the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group. At 2 weeks postoperatively, nerve fiber diameter, myelin thickness, and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group. These results indicate that Qian-Zheng-San has a positive effect on peripheral nerve regeneration.
Language	English
Publishing date	2019-01-15
Publishing country	India
Document type	Journal Article
ZDB-ID	2388460-5
ISSN	1876-7958 ; 1673-5374
ISSN (online)	1876-7958
ISSN	1673-5374
DOI	10.4103/1673-5374.247472
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Bu-Yin-Qian-Zheng Formula Ameliorates MPP

Ma, Hao-Jie / Gai, Cong / Chai, Yuan / Feng, Wan-Di / Cheng, Cui-Cui / Zhang, Jin-Kun / Zhang, Yu-Xin / Yang, Lu-Ping / Guo, Zhen-Yu / Gao, Yu-Shan / Sun, Hong-Mei

Frontiers in pharmacology

2020 Volume 11, Page(s) 577017

Abstract: As a typical traditional Chinese medicine, Bu-Yin-Qian-Zheng Formula (BYQZF) has been shown to have ...

Abstract	As a typical traditional Chinese medicine, Bu-Yin-Qian-Zheng Formula (BYQZF) has been shown to have neuroprotective effects in patients with Parkinson's disease (PD), particularly by ameliorating mitochondrial dysfunction and regulating expression of the parkin protein. However, the underlying mechanisms by which BYQZF affects mitochondrial function through parkin are unclear. Accordingly, in this study, we evaluated the mechanisms by which BYQZF ameliorates mitochondrial dysfunction through parkin in PD. We constructed a parkin-knockdown cell model and performed fluorescence microscopy to observe transfected SH-SY5Y cells. Quantitative real-time reverse transcription polymerase chain reaction and western blotting were conducted to detect the mRNA and protein expression levels of parkin. Additionally, we evaluated the cell survival rates, ATP levels, mitochondrial membrane potential (ΔΨm), mitochondrial morphology, parkin protein expression, PINK1 protein expression, and mitochondrial fusion and fission protein expression after treatment with MPP
Language	English
Publishing date	2020-12-18
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2020.577017
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Article ; Online: Reply to the Letter from Qian Ren and Yanjun Wang About the Article, "LI-RADS Morphological Type Predicts Prognosis of Patients with Hepatocellular Carcinoma After Radical Resection".

Zhang, Chunhui / Yang, Rui / Wang, Xinxin / Tao, Yuqing / Tang, Shuli / Tian, Zhennan / Zhou, Yang

Annals of surgical oncology

2023 Volume 31, Issue 3, Page(s) 1849–1850

MeSH term(s)	Humans ; Carcinoma, Hepatocellular/pathology ; Liver Neoplasms/pathology ; Contrast Media ; Magnetic Resonance Imaging ; Prognosis
Chemical Substances	Contrast Media
Language	English
Publishing date	2023-11-13
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	1200469-8
ISSN	1534-4681 ; 1068-9265
ISSN (online)	1534-4681
ISSN	1068-9265
DOI	10.1245/s10434-023-14557-2
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Article ; Online: In memoriam Steven Yue Qian (1960–2019)

Huiyong Yin / Garry Buettner / Daniel Tsun-Yee Chiu / XueJi Zhang / Chang Chen / Lin L. Mantell

Redox Biology, Vol 37, Iss , Pp 101821- (2020)

2020

Keywords	Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
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Article: Computational Systems Pharmacology, Molecular Docking and Experiments Reveal the Protective Mechanism of Li-Da-Qian Mixture in the Treatment of Glomerulonephritis.

Zhou, Wei / Sha, Yugen / Zeng, Jingxia / Zhang, Xiaoyue / Zhang, Aihua / Ge, Xuhua

Journal of inflammation research

2021 Volume 14, Page(s) 6939–6958

Abstract: ... suggests that traditional Chinese medicine has potential in treating glomerulonephritis, such as Li-Da-Qian ... the exact mechanism of Li-Da-Qian mixture devoting to glomerulonephritis remains unexplored. It was ... necessary to explore the mechanism of Li-Da-Qian mixture against glomerulonephritis using modern technology ...

Abstract	Background: Glomerulonephritis is a common urinary system disease among children. Growing evidence suggests that traditional Chinese medicine has potential in treating glomerulonephritis, such as Li-Da-Qian mixture. Although its anti-glomerulonephritis and alleviating hematuria effects have been reported, the exact mechanism of Li-Da-Qian mixture devoting to glomerulonephritis remains unexplored. It was necessary to explore the mechanism of Li-Da-Qian mixture against glomerulonephritis using modern technology, such as Chinese medicine database and molecular biological experiments. Methods: Online databases were used to look up ingredients and predict targets of Li-Da-Qian mixture against glomerulonephritis. The intersecting targets of Li-Da-Qian mixture and glomerulonephritis were selected for enrichment analysis. Cytoscape software was applied to establish network and MCODE analysis. Molecular docking was used for the primary validation. Furthermore, we examined the function of the core compounds analyzed from Li-Da-Qian mixture to rescue LPS-induced inflammation in vivo and vitro. We also explored whether the core compounds can alleviate TGFβ1-induced renal fibrosis in mouse proximal tubular cells. Results: Network pharmacological analysis of Li-Da-Qian evaluated 20 active ingredients including baicalein, luteolin and quercetin. A total of 113 key targets were screened, including IL6, VEGFA, TP53, EGF, MMP2, etc, and they were enriched in AGE-RAGE signaling pathway in diabetic complications, TNF and IL-17 signaling pathways. Moreover, the core ingredients succeeded in binding to the main targets via molecular docking, further identifying the anti-glomerulonephritis effects and improvement of vascular injury. Western blotting and qPCR also suggested that baicalein and luteolin can improve inflammation and restore disturbance of mesangial cells or kidney induced by LPS. In addition, baicalein and luteolin inhibited renal fibrosis in vitro.
Language	English
Publishing date	2021-12-16
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2494878-0
ISSN	1178-7031
ISSN	1178-7031
DOI	10.2147/JIR.S338055
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Article ; Online: Response to: 'Inflammation in SLE-PAH: good news or not?' by Junyan Qian

Sun, Fangfang / Lei, Yunxia / Zhang, Xiao / Ye, Shuang

Annals of the rheumatic diseases

2018 Volume 78, Issue 12, Page(s) e136

MeSH term(s)	Humans ; Hypertension, Pulmonary ; Inflammation ; Lupus Erythematosus, Systemic ; Phenotype ; Pulmonary Arterial Hypertension
Language	English
Publishing date	2018-11-14
Publishing country	England
Document type	Letter ; Comment
ZDB-ID	7090-7
ISSN	1468-2060 ; 0003-4967
ISSN (online)	1468-2060
ISSN	0003-4967
DOI	10.1136/annrheumdis-2018-214647
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Article ; Online: Effects of Fusu mixture (Wen-Shen-Qian-Yang Method) on sepsis-induced acute respiratory distress syndrome.

Zhang, Li / Long, Kunlan / Wang, Chunxia / Zhang, Xuemei / Yang, Hongjing / Chen, Jun / Li, Xue / Gao, Peiyang / Zhang, Song

Medicine

2020 Volume 99, Issue 29, Page(s) e21066

Abstract: Introduction: Sepsis is the most common etiology of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Capillary leakage caused by lung endothelial injury is the central cause of ARDS. The results of research in modern medicine in ... ...

Abstract	Introduction: Sepsis is the most common etiology of acute lung injury (ALI) or acute respiratory distress syndrome (ARDS). Capillary leakage caused by lung endothelial injury is the central cause of ARDS. The results of research in modern medicine in reducing endothelial damage and restoring endothelial functions are limited. In the previous clinical observations, we found that the Fusu mixture not only improves the clinical symptoms but also reduces the leakage of pulmonary capillaries. Therefore, the purpose of this study is to determine the clinical efficacy of the Fusu mixture combined with Western medicine in the treatment of ARDS caused by sepsis and to explore the mechanism of traditional Chinese medicine. Methods: This is a prospective, single-center, randomized, single-blind, and controlled clinical study involving 620 eligible patients. The patients will be randomly divided into 2 groups: the Western medicine treatment group and the combination of Chinese and Western medicine treatment group. After 14 days of intervention, the clinical efficacy and safety of the Fusu mixture on sepsis-induced ARDS patients will be observed. The primary outcome will be measured as 28-day mortality. The secondary outcome indices include inflammatory markers (CRP, PCT, IL-6, TNF - α), APACHE II score, SOFA score, days without a ventilator, blood gas analysis (Lac, PaO2 / FiO2), intensive care unit hospital stay time, intensive care unit mortality. Simultaneously, the analysis of the exploratory results will be carried out to analyze the possible mechanism of Fusu mixture in the treatment of sepsis-induced ARDS by the high-throughput sequencing and bioinformatics. Discussion: The purpose of this study is to evaluate the clinical efficacy of Fusu mixture in the treatment of sepsis-induced ARDS and explore its possible mechanism of action. If successful, it will provide evidence-based adjuvant therapy for the clinical treatment of ARDS.
MeSH term(s)	Adult ; Blood Gas Analysis ; Capillary Leak Syndrome/complications ; Capillary Leak Syndrome/etiology ; Female ; Humans ; Male ; Medicine, Chinese Traditional/methods ; Medicine, Chinese Traditional/standards ; Middle Aged ; Prospective Studies ; Respiratory Distress Syndrome/drug therapy ; Respiratory Distress Syndrome/etiology ; Respiratory Distress Syndrome/physiopathology ; Sepsis/complications ; Sepsis/drug therapy ; Sepsis/physiopathology ; Single-Blind Method ; Treatment Outcome
Language	English
Publishing date	2020-04-16
Publishing country	United States
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000021066
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Article ; Online: Computational Systems Pharmacology, Molecular Docking and Experiments Reveal the Protective Mechanism of Li-Da-Qian Mixture in the Treatment of Glomerulonephritis

Zhou W / Sha Y / Zeng J / Zhang X / Zhang A / Ge X

Journal of Inflammation Research, Vol Volume 14, Pp 6939-

2021 Volume 6958

Abstract: ... that traditional Chinese medicine has potential in treating glomerulonephritis, such as Li-Da-Qian mixture. Although its anti ... glomerulonephritis and alleviating hematuria effects have been reported, the exact mechanism of Li-Da-Qian mixture ... devoting to glomerulonephritis remains unexplored. It was necessary to explore the mechanism of Li-Da-Qian ...

Abstract	Wei Zhou,1,2,* Yugen Sha,2,* Jingxia Zeng,3,* Xiaoyue Zhang,4 Aihua Zhang,1,2 Xuhua Ge1,3 1Nanjing Key Laboratory of Pediatrics, Children’s Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Nephrology, Children’s Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 3Pediatric Intensive Care Unit, Children’s Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 4Department of Chinese Medicine, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xuhua GePediatric Intensive Care Unit, Children’s Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210029, People’s Republic of ChinaEmail gexuhua@njmu.edu.cnAihua ZhangDepartment of Nephrology, Children’s Hospital of Nanjing Medical University, 72 Guangzhou Road, Nanjing, 210029, People’s Republic of ChinaEmail zhaihua@njmu.edu.cnBackground: Glomerulonephritis is a common urinary system disease among children. Growing evidence suggests that traditional Chinese medicine has potential in treating glomerulonephritis, such as Li-Da-Qian mixture. Although its anti-glomerulonephritis and alleviating hematuria effects have been reported, the exact mechanism of Li-Da-Qian mixture devoting to glomerulonephritis remains unexplored. It was necessary to explore the mechanism of Li-Da-Qian mixture against glomerulonephritis using modern technology, such as Chinese medicine database and molecular biological experiments.Methods: Online databases were used to look up ingredients and predict targets of Li-Da-Qian mixture against glomerulonephritis. The intersecting targets of Li-Da-Qian mixture and glomerulonephritis were selected for enrichment analysis. Cytoscape software was applied to establish network and MCODE analysis. Molecular docking was used for the primary validation. Furthermore, we examined the function of the core compounds analyzed from Li-Da-Qian mixture to rescue LPS-induced inflammation in vivo and vitro. We also explored whether the core compounds can alleviate TGFβ 1-induced renal fibrosis in mouse proximal tubular cells.Results: Network pharmacological analysis of Li-Da-Qian evaluated 20 active ingredients including baicalein, luteolin and quercetin. A total of 113 key targets were screened, including IL6, VEGFA, TP53, EGF, MMP2, etc, and they were enriched in AGE-RAGE signaling pathway in diabetic complications, TNF and IL-17 signaling pathways. Moreover, the core ingredients succeeded in binding to the main targets via molecular docking, further identifying the anti-glomerulonephritis effects and improvement of vascular injury. Western blotting and qPCR also suggested that baicalein and luteolin can improve inflammation and restore disturbance of mesangial cells or kidney induced by LPS. In addition, baicalein and luteolin inhibited renal fibrosis in vitro.Keywords: glomerulonephritis, network pharmacology, Li-Da-Qian mixture, traditional Chinese medicine, molecular docking
Keywords	glomerulonephritis ; network pharmacology ; li-da-qian mixture ; traditional chinese medicine ; molecular docking ; Pathology ; RB1-214 ; Therapeutics. Pharmacology ; RM1-950
Subject code	950
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Dove Medical Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Qian Yang Yu Yin Granule protects against hypertension-induced renal injury by epigenetic mechanism linked to Nicotinamide N-Methyltransferase (NNMT) expression.

Zhang, Shu-Fei / Mao, Xin-Jing / Jiang, Wei-Min / Fang, Zhu-Yuan

Journal of ethnopharmacology

2020 Volume 255, Page(s) 112738

Abstract: Ethnopharmacological relevance: Qian Yang Yu Yin Granule (QYYY) is a Chinese herbal formulation ...

Abstract	Ethnopharmacological relevance: Qian Yang Yu Yin Granule (QYYY) is a Chinese herbal formulation. It is used to treat hypertensive nephropathy for decades in China, but it is unknown that the exact mechanism of QYYY on hypertensive nephropathy. Aims of study: The present study was to elucidate its epigenetic mechanism of QYYY on hypertensive nephropathy. Materials and methods: In the current study, HEK293T cells' proliferation induced by Ang II was chosen to observe epigenetic mechanisms of QYYY on renal damage. The cell proliferation was examined by MTT assays and ethynyldeoxyuridine analysis. Cell cycle analysis was performed. After treatment with QYYY, expression of Nicotinamide N-methyltransferase (NNMT), sirtuin1(SIRT1), S-adenosylhomocysteine(SAH), histone H3K4 methylation, and cortactin acetylation(acetyl-cortactin,ac-cortactin) were further investigated by western-blotting and real time PCR. DNA methylation was detected by ELISA. The study also observed the changes of SIRT1, SAH, H3K4 methylation, acetyl-cortactin when NNMT over-expressed by lentivirus transfection. Angiotensin II(Ang II) induced renal damage in spontaneously hypertensive rats(SHR). After eight weeks treatment of QYYY, blood pressure, serum and urine creatinine, and urinary microalbumin(mAlb) were assessed. The concentration of N1 -methylnicotinamide were detected by liquid chromatography with tandem mass spectrometry. The protein of NNMT, ac-cortactin, H3K3me3 were also assessed in vivo. Results: QYYY inhibited HEK293T cells' proliferation, down-regulated the expression of NNMT, SAH, acetyl-cortactin and DNA methylation, up-regulated the expression of SIRT1, histone H3K4 trimethylation(H3K4me3). Over-expression of NNMT increased the expression of SAH and acetyl-cortactin, and reduced the expression of SIRT1 and H3K4me3. The study also demonstrated that QYYY promoted urinary creatinine excretion and reduced serum creatinine and urinary mAlb in SHR. QYYY decreased the concentration of N1 -methylnicotinamide in Ang II group. QYYY decreased the protein of NNMT, ac-cortactin and increased H3K4me3 in vivo. Conclusion: The results showed that QYYY alleviated renal impairment of SHR and inhibited HEK293T cells' proliferation induced by Ang II through the pathway of epigenetic mechanism linked to Nicotinamide N-Methyltransferase (NNMT) expression, including histone methylation, DNA methylation and acetyl-cortactin. This study unveiled a novel molecular mechanism by which QYYY controlled the progression of hypertensive nephropathy.
MeSH term(s)	Acetylation ; Angiotensin II ; Animals ; Cell Proliferation/drug effects ; Cortactin/metabolism ; DNA Methylation/drug effects ; Disease Models, Animal ; Drugs, Chinese Herbal/pharmacology ; Epigenesis, Genetic/drug effects ; Epithelial Cells/drug effects ; Epithelial Cells/enzymology ; Epithelial Cells/pathology ; HEK293 Cells ; Histones/metabolism ; Humans ; Hypertension/complications ; Hypertension/drug therapy ; Hypertension/enzymology ; Hypertension/genetics ; Kidney/drug effects ; Kidney/enzymology ; Kidney/pathology ; Kidney Diseases/chemically induced ; Kidney Diseases/enzymology ; Kidney Diseases/genetics ; Kidney Diseases/prevention & control ; Male ; Nicotinamide N-Methyltransferase/metabolism ; Rats, Inbred SHR ; Rats, Inbred WKY ; S-Adenosylhomocysteine/metabolism ; Sirtuin 1/metabolism
Chemical Substances	CTTN protein, human ; Cortactin ; Drugs, Chinese Herbal ; Histones ; qian yang yu yin granule ; Angiotensin II (11128-99-7) ; S-Adenosylhomocysteine (979-92-0) ; NNMT protein, human (EC 2.1.1.1) ; Nicotinamide N-Methyltransferase (EC 2.1.1.1) ; Nnmt protein, rat (EC 2.1.1.1) ; SIRT1 protein, human (EC 3.5.1.-) ; Sirtuin 1 (EC 3.5.1.-)
Language	English
Publishing date	2020-03-05
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	134511-4
ISSN	1872-7573 ; 0378-8741
ISSN (online)	1872-7573
ISSN	0378-8741
DOI	10.1016/j.jep.2020.112738
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Article: Da-Bu-Yin-Wan and Qian-Zheng-San Ameliorate Mitochondrial Dynamics in the Parkinson's Disease Cell Model Induced by MPP

Gai, Cong / Feng, Wan-Di / Qiang, Tian-Yao / Ma, Hao-Jie / Chai, Yuan / Zhang, Shu-Jing / Guo, Zhen-Yu / Hu, Jing-Hong / Sun, Hong-Mei

Frontiers in pharmacology

2019 Volume 10, Page(s) 372

Abstract: To investigate the effect of Da-Bu-Yin-Wan and Qian-Zheng-San (DBYW and QZS) on mitochondrial mass ...

Abstract	To investigate the effect of Da-Bu-Yin-Wan and Qian-Zheng-San (DBYW and QZS) on mitochondrial mass in Parkinson's disease (PD) cell model induced by 1-Methyl-4-phenylpyridinium Ion (MPP
Language	English
Publishing date	2019-04-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587355-6
ISSN	1663-9812
ISSN	1663-9812
DOI	10.3389/fphar.2019.00372
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