LIVIVO - Search results -

Search results

Result 1 - 10 of total 14498

Search options

Article ; Online: Rapid Detection of SARS-CoV-2 in Clinical and Environmental Samples via a Resonant Cavity SERS Platform within 20 min.

Huang, Jinglin / Wang, Conghui / Wang, Pingshi / Mo, Wenbo / Zhou, Minjie / Le, Wei / Qi, Daojian / Wei, Lai / Fan, Quanping / Yang, Yue / Ni, Shuang / Wu, Yan / Feng, Yuliang / Wang, Xiang / Zhao, Zongqing / He, Zhibing / Zhang, Haijun / Xue, Peili / Ren, Bin /

Ren, Lili / Pan, Ming / Du, Kai

ACS applied materials & interfaces

2023

Abstract: ... and rapid detection of SARS-CoV-2 antigen within 20 min by the combination of a highly consistent SERS ...

Abstract	The coronavirus disease 2019 (COVID-19) epidemic has given a warning that it is important to explore the rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical specimens or environmental samples for public health strategies and future variants. The surface-enhanced Raman spectroscopy (SERS) technique was demonstrated to achieve this goal. However, the consistency of signals originating from the poor compatibility of virions with SERS hotspots remains a key scientific challenge for the practical applications of SERS. Herein, we develop a SERS platform for the ultrasensitive and rapid detection of SARS-CoV-2 antigen within 20 min by the combination of a highly consistent SERS substrate and a supervised deep learning algorithm. A V-shaped resonant cavity array (VRC) substrate was fabricated to trap SARS-CoV-2 virions in the periodic V cavity array and stimulate the integral SERS signal of the virus via a resonance coupling effect. Benefiting from the unique architecture of the VRC substrate, we were able to directly detect the SARS-CoV-2 virus with high sensitivity and high consistency. These excellent performances enabled us to identify five different kinds of SARS-CoV-2 variants and detect SARS-CoV-2 from clinical and environmental samples with high accuracies.
Language	English
Publishing date	2023-10-25
Publishing country	United States
Document type	Journal Article
ISSN	1944-8252
ISSN (online)	1944-8252
DOI	10.1021/acsami.3c08819
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Frequency modulation of the Min-protein oscillator by nucleoid-associated factors in Escherichia coli.

Shen, Jie-Pan / Chang, Yi-Ren / Chou, Chia-Fu

Biochemical and biophysical research communications

2020 Volume 525, Issue 4, Page(s) 857–862

Abstract: In E. coli, the Min-protein oscillator, together with the nucleoid occlusion (NO), destabilizes ... are thought to be working independently for division site placement. Even though the Min-protein ... oscillator has been displayed by synthetic minimal systems, it is unclear the interplays of Min proteins and ...

Abstract	In E. coli, the Min-protein oscillator, together with the nucleoid occlusion (NO), destabilizes the Z-ring assembly away from the midcell to ensure faithful septation. These two inhibitory pathways are thought to be working independently for division site placement. Even though the Min-protein oscillator has been displayed by synthetic minimal systems, it is unclear the interplays of Min proteins and compartment geometry are sufficient to bolster oscillation stability in vivo. By probing if NO plays a role in the Min oscillation, we study the oscillation frequency in the anucleate and nucleoid-perturbed cells. Surprisingly, we found that the oscillation periods of the Min-protein oscillators were seriously deviated in the anucleate and nucleoid-perturbed cells, but the oscillation frequency either went up in the anucleate or down in the nucleoid-perturbed cells. Intriguingly, enhanced stability and reduced frequency were observed in the cells expressing the NO factor SlmA higher than the native level. Our results reveal an unanticipated role of the nucleoid in modulating the frequency and stability of Min-protein system. SlmA is indicated to facilitate such modulations, potentially via directly interacting with the Min-protein system. A fresh perspective is suggested that frequency modulation of Min-protein oscillator is mediated via the act of nucleoid-associated factors.
MeSH term(s)	Adenosine Triphosphatases/genetics ; Adenosine Triphosphatases/metabolism ; Carrier Proteins/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cell Division ; Chromosomal Proteins, Non-Histone/genetics ; Escherichia coli/cytology ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Microorganisms, Genetically-Modified ; Mutation
Chemical Substances	Carrier Proteins ; Cell Cycle Proteins ; Chromosomal Proteins, Non-Histone ; Escherichia coli Proteins ; MinE protein, E coli ; MukB protein, E coli ; SlmA protein, E coli ; Adenosine Triphosphatases (EC 3.6.1.-) ; MinD protein, E coli (EC 3.6.1.-)
Language	English
Publishing date	2020-03-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Video-Audio Media
ZDB-ID	205723-2
ISSN	1090-2104 ; 0006-291X ; 0006-291X
ISSN (online)	1090-2104 ; 0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2020.02.161
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 116: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: On the min–max estimation of mean daily temperatures

Tsonis, Anastasios A / Pan, Xinnong / Wang, Geli / Nicolis, Catherine

Climate dynamics. 2019 Aug., v. 53, no. 3-4

2019

Abstract: ... is solved analytically and shown to induce substantial deviations from the min–max method that depend ...

Abstract	Modern data analyses of hourly temperature records reveal the existence, in addition to the daily cycle, of multiple forcings of different frequencies. As a result the routine approach of estimating daily local mean temperature directly from the average of the minimum and maximum is heavily compromised. A simple dynamical model subjected to two periodic forcings of different frequencies, amplitudes and phases is solved analytically and shown to induce substantial deviations from the min–max method that depend crucially on the values of the parameters involved.
Keywords	climate ; dynamic models ; temperature
Language	English
Dates of publication	2019-08
Size	p. 1981-1989.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	1471747-5
ISSN	1432-0894 ; 0930-7575
ISSN (online)	1432-0894
ISSN	0930-7575
DOI	10.1007/s00382-019-04757-6
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Integrating filter paper extraction, isothermal amplification, and lateral flow dipstick methods to detect Streptococcus agalactiae in milk within 15 min

Lingling Zhu / Fengju Gong / Xia Liu / Xueqiang Sun / Yong Yu / Jie Shu / Zihao Pan

Frontiers in Veterinary Science, Vol

2023 Volume 10

Abstract: ... The entire method takes only 15 min, is low in cost, has high precision and low technical requirements ...

Abstract	IntroductionMastitis is one of the most serious diseases affecting dairy farming, causing huge economic losses worldwide. Streptococcus agalactiae is the main pathogenic bacterium of contagious mastitis and can deliver a devastating blow to a farm's economy. Rapid detection is the key to disease control.MethodsIn this study, a rapid detection method for S. agalactiae was established. This method combines filter paper extraction, multienzyme isothermal rapid amplification (MIRA), and lateral flow dipsticks (LFD). To simplify the extraction procedure, we designed a disposable extraction device (DED). First, DED performance was evaluated by polymerase chain reaction (PCR) and then the lysis formula and extraction time were optimized. Second, this study compared the extraction performance of a filter paper and an automatic nucleic acid extraction instrument. After screening primers, MIRA for S. agalactiae was established and combined with LFD. Specificity and sensitivity were evaluated after optimizing the reaction conditions.ResultsThe results showed that the lowest extraction line for DED was 0.01–0.001 ng/μl. In the specificity study, 12 different bacteria were tested, and only S. agalactiae was found to be positive. In the sensitivity study, seven dilution gradients were established, and the lowest detection line was 3.52 × 102 CFU/ml.DiscussionIn summary, the method established in this study does not require laboratory equipment and is suitable for on-site detection. The entire method takes only 15 min, is low in cost, has high precision and low technical requirements for operators, which is in contrast with the high cost and cumbersome operation of traditional methods, and is suitable for on-site testing in areas with limited facilities.
Keywords	Streptococcus agalactiae ; filter paper extraction ; isothermal amplification ; lateral flow dipsticks ; rapid detection ; Veterinary medicine ; SF600-1100
Subject code	630
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Frequency modulation of the Min-protein oscillator by nucleoid-associated factors in Escherichia coli

Shen, Jie-Pan / Chang, Yi-Ren / Chou, Chia-Fu

Biochemical and biophysical research communications. 2020 May 14, v. 525, no. 4

2020

Abstract	In E. coli, the Min-protein oscillator, together with the nucleoid occlusion (NO), destabilizes the Z-ring assembly away from the midcell to ensure faithful septation. These two inhibitory pathways are thought to be working independently for division site placement. Even though the Min-protein oscillator has been displayed by synthetic minimal systems, it is unclear the interplays of Min proteins and compartment geometry are sufficient to bolster oscillation stability in vivo. By probing if NO plays a role in the Min oscillation, we study the oscillation frequency in the anucleate and nucleoid-perturbed cells. Surprisingly, we found that the oscillation periods of the Min-protein oscillators were seriously deviated in the anucleate and nucleoid-perturbed cells, but the oscillation frequency either went up in the anucleate or down in the nucleoid-perturbed cells. Intriguingly, enhanced stability and reduced frequency were observed in the cells expressing the NO factor SlmA higher than the native level. Our results reveal an unanticipated role of the nucleoid in modulating the frequency and stability of Min-protein system. SlmA is indicated to facilitate such modulations, potentially via directly interacting with the Min-protein system. A fresh perspective is suggested that frequency modulation of Min-protein oscillator is mediated via the act of nucleoid-associated factors.
Keywords	Escherichia coli ; geometry ; research
Language	English
Dates of publication	2020-0514
Size	p. 857-862.
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	205723-2
ISSN	0006-291X ; 0006-291X
ISSN (online)	0006-291X
ISSN	0006-291X
DOI	10.1016/j.bbrc.2020.02.161
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 71/706: Show issues
Z 3.8: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Oxygen desaturation during the 6-min walk test as a risk for osteoporosis in non-cystic fibrosis bronchiectasis.

Huang, Hung-Yu / Sheng, Te-Fang / Lin, Chang-Wei / Wang, Ting-Wen / Lo, Chun-Yu / Chung, Fu-Tsai / Yang, Lan-Yan / Pan, Yu-Bin / Wang, Chun-Hua

BMC pulmonary medicine

2019 Volume 19, Issue 1, Page(s) 28

Abstract: ... patients. We determined whether desaturation during 6-min walk test (6MWT) can be a predictor ...

Abstract	Background: Osteoporosis is a common comorbidity in non-cystic fibrosis (non-CF) bronchiectasis patients. We determined whether desaturation during 6-min walk test (6MWT) can be a predictor for osteoporosis risk. Methods: This was a retrospective cross-sectional study. Sixty-six non-CF bronchiectasis patients were enrolled. Lung function, walking distance, the lowest oxygen saturation (SpO Results: Osteoporosis was evident in more desaturators (82%) than non-desaturators (43%, p < 0.01). BMD at the level of the femoral neck was significantly lower in desaturators than in non-desaturators (- 3.6 ± 1.1 vs. - 2.4 ± 0.9, p < 0.01). BMD was correlated positively with the lowest SpO Conclusions: Exertional desaturation during the 6MWT was a significant predictive factor for osteoporosis in Asian non-CF bronchiectasis patients. The 6MWT may be useful in identifying the osteoporotic phenotype of non-CF bronchiectasis and increasing clinician awareness to promote early intervention.
MeSH term(s)	Aged ; Bone Density ; Bronchiectasis/complications ; Bronchiectasis/physiopathology ; Comorbidity ; Cross-Sectional Studies ; Exercise Tolerance ; Female ; Forced Expiratory Volume ; Humans ; Linear Models ; Male ; Middle Aged ; Multivariate Analysis ; Osteoporosis/complications ; Osteoporosis/diagnosis ; Oxygen/blood ; Predictive Value of Tests ; Retrospective Studies ; Risk Factors ; Taiwan ; Tomography, X-Ray Computed ; Walk Test
Chemical Substances	Oxygen (S88TT14065)
Language	English
Publishing date	2019-02-04
Publishing country	England
Document type	Journal Article
ZDB-ID	2059871-3
ISSN	1471-2466 ; 1471-2466
ISSN (online)	1471-2466
ISSN	1471-2466
DOI	10.1186/s12890-019-0794-x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: A Novel Rapid Method for Viewshed Computation on DEM through Max-Pooling and Min-Expected Height

Pan, Zhibin / Tang, Jin / Tjahjadi, Tardi / Wu, Zhihu / Xiao, Xiaoming

ISPRS international journal of geo-information. 2020 Oct. 26, v. 9, no. 11

2020

Abstract: Viewshed computation of a digital elevation model (DEM) plays an important role in a geographic information system, but the required high computational time is a serious problem for a practical application. Hitherto, the mainstream methods of viewshed ... ...

Abstract	Viewshed computation of a digital elevation model (DEM) plays an important role in a geographic information system, but the required high computational time is a serious problem for a practical application. Hitherto, the mainstream methods of viewshed computing include line-of-sight method, reference planes method, etc. Based on these classical algorithms, a new algorithm for viewshed computation is proposed in this paper: the Matryoshka doll algorithm. Through a pooling operation, the minimum expected height of the DEM is introduced as max-pooling with minimum expected height in the viewshed computing optimization. This is to increase the efficiency and adaptability of the computation of the visibility range. The experimental results demonstrate that the algorithm has obvious advantages in computing speed, but with the accuracy only slightly reduced.
Keywords	accuracy ; algorithms ; digital elevation models ; geographic information systems ; height ; paper ; rapid methods ; spatial data
Language	English
Dates of publication	2020-1026
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ZDB-ID	2655790-3
ISSN	2220-9964
ISSN	2220-9964
DOI	10.3390/ijgi9110633
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: A Novel Rapid Method for Viewshed Computation on DEM through Max-Pooling and Min-Expected Height

Zhibin Pan / Jin Tang / Tardi Tjahjadi / Zhihu Wu / Xiaoming Xiao

ISPRS International Journal of Geo-Information, Vol 9, Iss 633, p

2020 Volume 633

Abstract	Viewshed computation of a digital elevation model (DEM) plays an important role in a geographic information system, but the required high computational time is a serious problem for a practical application. Hitherto, the mainstream methods of viewshed computing include line-of-sight method, reference planes method, etc. Based on these classical algorithms, a new algorithm for viewshed computation is proposed in this paper: the Matryoshka doll algorithm. Through a pooling operation, the minimum expected height of the DEM is introduced as max-pooling with minimum expected height in the viewshed computing optimization. This is to increase the efficiency and adaptability of the computation of the visibility range. The experimental results demonstrate that the algorithm has obvious advantages in computing speed, but with the accuracy only slightly reduced.
Keywords	viewshed computation ; DEM ; max-pooling ; minimum expected height ; Geography (General) ; G1-922
Subject code	518
Language	English
Publishing date	2020-10-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: [Distribution Characteristics of Sedimentary Pigments in the Changjiang Estuary and Zhe-Min Coast and its Implications].

Li, Dong / Yao, Peng / Zhao, Bin / Wang, Jin-peng / Pan, Hui-hui

Huan jing ke xue= Huanjing kexue

2015 Volume 36, Issue 8, Page(s) 2791–2799

Abstract: ... from Changjiang Diluted Water and upwelling in the Zhe-Min coastal mud area were the major cause for the patchy ... exhibited high values in the Changjiang Estuary and Zhe-Min Coasts. Based on the relative proportions ... and upwelling region in the Zhe-Min Coast) were mainly due to the higher sedimentation rate and ...

Abstract	Compositions and contents of sedimentary pigments were examined using high performance liquid chromatography in order to discuss the spatial distributions of phytoplankton primary production, phytoplankton functional type and the preservation efficiency of phytoplankton pigments and their influencing factors. The results showed that: chloropigments [Chlorins, including chlorophyll-a (Chl-a) and pheopigments (Pheo-a), such as pheophytin-a (PHtin-a), pheophorbide-a (PHide-a), pPheophytin-a (pPHtin-a), sterol chlorin esters (SCEs) and carotenol chlorin esters (CCEs)] were the major type of sedimentary pigments. The nutrients inputs from Changjiang Diluted Water and upwelling in the Zhe-Min coastal mud area were the major cause for the patchy distribution with high sedimentary chloropigment contents. Carotenoid contents showed no trending changes and exhibited high values in the Changjiang Estuary and Zhe-Min Coasts. Based on the relative proportions of each diagnostic carotenoid to the total diagnostic carotenoids in the sediments, the relative contributions of diatoms, dinoflagellates, prymnesiophytes, prasinophytes, cryptophytes and cyanobacterias in the phytoplankton fuctional types were 48.8% +/- 17.4%, 10.7% +/- 11.5%, 8.1% +/- 7.2%, 18.6% +/- 8.2%, 9.4% +/- 6.4% and 4.3% +/- 3.2%, respectively. The preference for external environmental conditions (e.g., nutrient level and water salinity) was the main cause for the decreasing trends of diatoms and dinoflagellates proportions and the increasing trends of prasinophytes, cryptophytes and cyanobacterias seawards. Based on the spatial distribution of Chl-a/Pheo-a ratios, the higher preservation efficiencies of sedimentary pigments in the coastal regions (e.g., outer edge of maximum turbidity zone in the Changjiang Estuary, mouth of the Hangzhou Bay and upwelling region in the Zhe-Min Coast) were mainly due to the higher sedimentation rate and seasonal occurrences of hypoxia in bottom water, and these regions with higher sedimentary pigment preservation efficiencies were probably ideal areas for the marine eco-environmental evolutions. The bad sedimentary environment caused by the water exchange inside and outside of Hangzhou Bay was the dominant reason for the low sedimentary pigment contents and preservation efficiencies in this region.
MeSH term(s)	Carotenoids/analysis ; China ; Chlorophyll/analogs & derivatives ; Chlorophyll/analysis ; Cryptophyta ; Diatoms ; Dinoflagellida ; Estuaries ; Geologic Sediments/chemistry ; Haptophyta ; Phytoplankton ; Porphyrins/analysis ; Water
Chemical Substances	Porphyrins ; Water (059QF0KO0R) ; Chlorophyll (1406-65-1) ; chlorin (2683-84-3) ; Carotenoids (36-88-4) ; pheophorbide a (IA2WNI2HO2) ; chlorophyll a (YF5Q9EJC8Y)
Language	Chinese
Publishing date	2015-08
Publishing country	China
Document type	Journal Article
ISSN	0250-3301
ISSN	0250-3301
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The effects of prosodic boundaries on nasality in Taiwan Min.

Pan, Ho-hsien

The Journal of the Acoustical Society of America

2007 Volume 121, Issue 6, Page(s) 3755–3769

Abstract: This study explores the effects of prosodic boundaries on nasality at intonational phrase, word, and syllable boundaries. The subjects were recorded saying phrases that contained a syllable-final nasal consonant followed by a syllable-initial stop. The ... ...

Abstract	This study explores the effects of prosodic boundaries on nasality at intonational phrase, word, and syllable boundaries. The subjects were recorded saying phrases that contained a syllable-final nasal consonant followed by a syllable-initial stop. The timing, duration, and magnitude of the nasal airflows measured were used to determine the extent of nasality across boundaries. Nasal amplitudes were found to vary in a speaker-dependent manner among boundary types. However, the patterns of nasal contours and temporal aspects of the airflow parameters consistently varied with boundary type across all the speakers. In general, the duration of nasal airflow and nasal plateau were the longest at the intonational phrase boundary, followed by word boundary and then syllable boundary. In addition to the hierarchical influence of boundary strength, there were unique phonetic markings associated with individual boundaries. In particular, two nasal rises interrupted by nasal inhalation occurred only across an intonation phrase boundary. Also, unexpectedly, a word boundary was marked by the longest postboundary vowel, whereas a syllable boundary was marked with the shortest nasal duration. The results here support the hierarchical effect of boundary on both domain-edge strengthening and cross-boundary coarticulation.
MeSH term(s)	Adult ; China/ethnology ; Humans ; Inhalation/physiology ; Language ; Male ; Mouth/anatomy & histology ; Mouth/physiology ; Nose/anatomy & histology ; Nose/physiology ; Speech/physiology ; Taiwan ; Voice/physiology
Language	English
Publishing date	2007-06
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	219231-7
ISSN	1520-8524 ; 0001-4966
ISSN (online)	1520-8524
ISSN	0001-4966
DOI	10.1121/1.2722208
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 591: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito