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  1. Article ; Online: A review of front-of-pack nutrition labelling in Southeast Asia: Industry interference, lessons learned, and future directions.

    Pettigrew, Simone / Coyle, Daisy / McKenzie, Briar / Vu, Duong / Lim, Shiang Cheng / Berasi, Kyra / Poowanasatien, Amphika / Suya, Inthira / Kowal, Paul

    The Lancet regional health. Southeast Asia

    2022  Volume 3, Page(s) 100017

    Abstract: Front-of-pack nutrition labelling is an evidence-based nutrition intervention that is recommended by the World Health Organization and other health agencies as an effective non-communicable disease prevention strategy. To date, the types of front-of-pack ...

    Abstract Front-of-pack nutrition labelling is an evidence-based nutrition intervention that is recommended by the World Health Organization and other health agencies as an effective non-communicable disease prevention strategy. To date, the types of front-of-pack labels that have been identified as being most effective have yet to be implemented in Southeast Asia. This has been partly attributed to extensive industry interference in nutrition policy development and implementation. This paper outlines the current state of food labelling policy in the region, describes observed industry interference tactics, and provides recommendations for how governments in Southeast Asia can address this interference to deliver best-practice nutrition labelling to improve diets at the population level. The experiences of four focal countries - Malaysia, Thailand, the Philippines, and Viet Nam - are highlighted to provide insights into the range of industry tactics that are serving to prevent optimal food labelling policies from being developed and implemented.
    Funding: This research was supported by the United Kingdom Global Better Health Programme, which is managed by the United Kingdom Foreign, Commonwealth and Development Office and supported by PricewaterhouseCoopers in Southeast Asia.
    Language English
    Publishing date 2022-06-13
    Publishing country England
    Document type Journal Article ; Review
    ISSN 2772-3682
    ISSN (online) 2772-3682
    DOI 10.1016/j.lansea.2022.05.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Real-time digital polymerase chain reaction (PCR) as a novel technology improves limit of detection for rare allele assays.

    Xu, Jiachen / Duong, Kyra / Yang, Zhenlin / Kaji, Kavanaugh / Ou, Jiajia / Head, Steven R / Crynen, Gogce / Ordoukhanian, Phillip / Hanna, Lauren / Hanna, Ava / Wang, Yan / Wang, Zhijie / Wang, Jie

    Translational lung cancer research

    2022  Volume 10, Issue 12, Page(s) 4336–4352

    Abstract: Background: Tumor heterogeneity may lead to false negative test results for tissue biopsy-based companion diagnostic tests. Real-time polymerase chain reaction (PCR) and digital PCR assays are used to detect rare alleles in cell-free circulating DNA for ...

    Abstract Background: Tumor heterogeneity may lead to false negative test results for tissue biopsy-based companion diagnostic tests. Real-time polymerase chain reaction (PCR) and digital PCR assays are used to detect rare alleles in cell-free circulating DNA for liquid biopsies; however, those tests lack strong sensitivity at low allele frequencies. We show here a novel real-time digital PCR instrument that utilizes cycle-based amplification curves to further improve the sensitivity and quantification accuracy of digital PCR.
    Methods: The novel real-time digital PCR instrument was compared to an endpoint digital PCR system to determine the sensitivity and quantification accuracy of both instruments. Samples were all thermal cycled on the real-time digital PCR instrument but were analyzed on both endpoint and real-time digital PCR instruments to compare the performance without introducing other variables. Contrived samples for epidermal growth factor receptor (
    Results: By removing false positive datapoints using real-time amplification curves, real-time digital PCR improved sensitivity by lowering the baseline for wildtype samples. For
    Conclusions: This novel technology with improved sensitivity is important and needed because it addresses current issues with liquid biopsy tests. Due to limited amounts of circulating tumor DNA (ctDNA) obtained for liquid biopsy tests, few copies of mutant alleles are expected. With the lower baseline of real-time digital PCR, false negative test results from tissue biopsy would be more effectively reduced, leading to more patients receiving the targeted therapy they need for better survival.
    Language English
    Publishing date 2022-01-08
    Publishing country China
    Document type Journal Article
    ZDB-ID 2754335-3
    ISSN 2226-4477 ; 2218-6751
    ISSN (online) 2226-4477
    ISSN 2218-6751
    DOI 10.21037/tlcr-21-728
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Increased sensitivity using real-time dPCR for detection of SARS-CoV-2.

    Duong, Kyra / Ou, Jiajia / Li, Zhaoliang / Lv, Zhaoqing / Dong, Hao / Hu, Tao / Zhang, Yunyun / Hanna, Ava / Gordon, Skyler / Crynen, Gogce / Head, Steven R / Ordoukhanian, Phillip / Wang, Yan

    BioTechniques

    2020  Volume 70, Issue 1, Page(s) 7–20

    Abstract: A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some ... ...

    Abstract A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.
    MeSH term(s) COVID-19/diagnosis ; COVID-19 Nucleic Acid Testing/methods ; COVID-19 Nucleic Acid Testing/statistics & numerical data ; Endpoint Determination ; Humans ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2/isolation & purification
    Language English
    Publishing date 2020-11-23
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 48453-2
    ISSN 1940-9818 ; 0736-6205
    ISSN (online) 1940-9818
    ISSN 0736-6205
    DOI 10.2144/btn-2020-0133
    Database MEDical Literature Analysis and Retrieval System OnLINE

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