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  1. Article ; Online: Coding-complete genomic sequence of a rhinovirus C-32 in a human nasal swab sample that tested false positive in a SARS-CoV-2 antigen test.

    Moquin, Tracey L / Subramaniam, Kuttichantran / Lednicky, John A

    Microbiology resource announcements

    2024  Volume 13, Issue 4, Page(s) e0017224

    Abstract: Rhinovirus-A was previously shown to cause false-positive results in a Japanese SARS-CoV-2 antigen test. We report that a false-positive result was obtained in a specimen with rhinovirus C-32 that had been tested using an American SARS-CoV-2 antigen test. ...

    Abstract Rhinovirus-A was previously shown to cause false-positive results in a Japanese SARS-CoV-2 antigen test. We report that a false-positive result was obtained in a specimen with rhinovirus C-32 that had been tested using an American SARS-CoV-2 antigen test.
    Language English
    Publishing date 2024-03-25
    Publishing country United States
    Document type Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.00172-24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Practical and Economic Approach for Assessing Potential Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Transmission Risk in Coronavirus Disease 2019 (COVID-19) Patients.

    Lednicky, John A

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2020  Volume 73, Issue 9, Page(s) e2867–e2868

    MeSH term(s) COVID-19 ; Humans ; Middle East Respiratory Syndrome Coronavirus ; SARS-CoV-2
    Language English
    Publishing date 2020-12-01
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/ciaa1789
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1505: Show issues Location:
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    Zs.MO 480: Show issues

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  3. Article: Inactivation of SARS CoV-2 on porous and nonporous surfaces by compact portable plasma reactor.

    Choudhury, Bhaswati / Lednicky, John A / Loeb, Julia C / Portugal, Sherlie / Roy, Subrata

    Frontiers in bioengineering and biotechnology

    2024  Volume 12, Page(s) 1325336

    Abstract: We report the inactivation of SARS CoV-2 and its surrogate-Human coronavirus OC43 (HCoV-OC43), on representative porous (KN95 mask material) and nonporous materials (aluminum and polycarbonate) using a Compact Portable Plasma Reactor (CPPR). The CPPR is ... ...

    Abstract We report the inactivation of SARS CoV-2 and its surrogate-Human coronavirus OC43 (HCoV-OC43), on representative porous (KN95 mask material) and nonporous materials (aluminum and polycarbonate) using a Compact Portable Plasma Reactor (CPPR). The CPPR is a compact (48 cm
    Language English
    Publishing date 2024-02-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2719493-0
    ISSN 2296-4185
    ISSN 2296-4185
    DOI 10.3389/fbioe.2024.1325336
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  4. Article ; Online: Environmental sampling for disease surveillance: Recent advances and recommendations for best practice.

    Stevenson, Eric / Mortazavi, Roya / Casuccio, Gary S / Chow, Judith C / Lednicky, John A / Lee, Richard J / Levine, Alan / Watson, John G

    Journal of the Air & Waste Management Association (1995)

    2023  Volume 73, Issue 10, Page(s) 723–729

    Language English
    Publishing date 2023-09-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1003064-5
    ISSN 2162-2906 ; 0894-0630 ; 1047-3289 ; 1096-2247
    ISSN (online) 2162-2906
    ISSN 0894-0630 ; 1047-3289 ; 1096-2247
    DOI 10.1080/10962247.2023.2253709
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 748: Show issues Location:
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  5. Article: Imported Dengue Case Numbers and Local Climatic Patterns Are Associated with Dengue Virus Transmission in Florida, USA.

    Stephenson, Caroline / Coker, Eric / Wisely, Samantha / Liang, Song / Dinglasan, Rhoel R / Lednicky, John A

    Insects

    2022  Volume 13, Issue 2

    Abstract: ... Aedes ... ...

    Abstract Aedes aegypti
    Language English
    Publishing date 2022-02-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662247-6
    ISSN 2075-4450
    ISSN 2075-4450
    DOI 10.3390/insects13020163
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  6. Article ; Online: Ecology and public health burden of Keystone virus in Florida.

    Henry, Christopher J / Pillai, Alexander N / Lednicky, John A / Morris, J Glenn / Hladish, Thomas J

    Epidemics

    2022  Volume 39, Page(s) 100555

    Abstract: Keystone virus (KEYV) is an under-studied orthobunyavirus that is transmitted via both horizontal and vertical cycles involving various mosquito species and vertebrate hosts. Historical evidence indicates that KEYV causes sub-clinical infections in ... ...

    Abstract Keystone virus (KEYV) is an under-studied orthobunyavirus that is transmitted via both horizontal and vertical cycles involving various mosquito species and vertebrate hosts. Historical evidence indicates that KEYV causes sub-clinical infections in humans, and some case studies draw links between this virus and encephalitis. Given KEYV's potential to cause human infections, it is plausible that it causes an under-appreciated proportion of both generic viral infections and unidentified viral encephalitis cases. This review summarizes current knowledge of KEYV and its disease dynamics in order to better understand the virus' medical and economic burden on human populations.
    MeSH term(s) Animals ; Florida/epidemiology ; Humans ; Public Health
    Language English
    Publishing date 2022-03-22
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2467993-8
    ISSN 1878-0067 ; 1755-4365
    ISSN (online) 1878-0067
    ISSN 1755-4365
    DOI 10.1016/j.epidem.2022.100555
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Mayaro virus detection by integrating sample preparation with isothermal amplification in portable devices.

    Alipanah, Morteza / Manzanas, Carlos / Hai, Xin / Lednicky, John A / Paniz-Mondolfi, Alberto / Morris, J Glenn / Fan, Z Hugh

    Analytical and bioanalytical chemistry

    2023  Volume 415, Issue 23, Page(s) 5605–5617

    Abstract: Mayaro virus (MAYV) is an emerging mosquito-borne alphavirus that causes clinical symptoms similar to those caused by Chikungunya virus (CHIKV), Dengue virus (DENV), and Zika virus (ZIKV). To differentiate MAYV from these viruses diagnostically, we have ... ...

    Abstract Mayaro virus (MAYV) is an emerging mosquito-borne alphavirus that causes clinical symptoms similar to those caused by Chikungunya virus (CHIKV), Dengue virus (DENV), and Zika virus (ZIKV). To differentiate MAYV from these viruses diagnostically, we have developed a portable device that integrates sample preparation with real-time, reverse-transcription, loop-mediated isothermal amplification (rRT-LAMP). First, we designed a rRT-LAMP assay targeting MAYV's non-structural protein (NS1) gene and determined the limit of detection of at least 10 viral genome equivalents per reaction. The assay was specific for MAYV, without cross-reactions with CHIKV, DENV, or ZIKV. The rRT-LAMP assay was integrated with a sample preparation device (SPD) wherein virus lysis and RNA enrichment/purification were carried out on the spot, without requiring pipetting, while subsequent real-time amplification device (RAD) enables virus detection at the point of care (POC). The functions of our platform were demonstrated using purified MAYV RNA or blood samples containing viable viruses. We have used the devices for detection of MAYV in as short as 13 min, with limit of detection to as low as 10 GEs/reaction.
    MeSH term(s) Animals ; Humans ; Zika Virus Infection/diagnosis ; Zika Virus/genetics ; Chikungunya virus/genetics ; Nucleic Acid Amplification Techniques ; Genome, Viral ; RNA, Viral/genetics
    Chemical Substances RNA, Viral
    Language English
    Publishing date 2023-07-20
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 201093-8
    ISSN 1618-2650 ; 0016-1152 ; 0372-7920
    ISSN (online) 1618-2650
    ISSN 0016-1152 ; 0372-7920
    DOI 10.1007/s00216-023-04856-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 4' 69/97: Show issues
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  8. Article ; Online: Coronaviruses: Facts, Myths, and Hypotheses.

    Carbone, Michele / Green, Joshua B / Bucci, Enrico M / Lednicky, John A

    Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer

    2020  Volume 15, Issue 5, Page(s) 675–678

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus ; Coronavirus Infections ; Humans ; Lung Neoplasms ; Pandemics ; Pneumonia ; Pneumonia, Viral ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-03-07
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 2432037-7
    ISSN 1556-1380 ; 1556-0864
    ISSN (online) 1556-1380
    ISSN 1556-0864
    DOI 10.1016/j.jtho.2020.02.024
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6664: Show issues Location:
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    Zs.MO 652: Show issues

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  9. Article ; Online: Transmission Potential of Floridian Aedes aegypti Mosquitoes for Dengue Virus Serotype 4: Implications for Estimating Local Dengue Risk.

    Stephenson, Caroline J / Coatsworth, Heather / Kang, Seokyoung / Lednicky, John A / Dinglasan, Rhoel R

    mSphere

    2021  Volume 6, Issue 4, Page(s) e0027121

    Abstract: Dengue virus serotype 4 (DENV-4) circulated in Aedes aegypti in 2016 and 2017 in Florida in the absence of human index cases, compelling a full assessment of local mosquito vector competence and DENV-4 risk. To better understand DENV-4 transmission risk ... ...

    Abstract Dengue virus serotype 4 (DENV-4) circulated in Aedes aegypti in 2016 and 2017 in Florida in the absence of human index cases, compelling a full assessment of local mosquito vector competence and DENV-4 risk. To better understand DENV-4 transmission risk in Florida, we used an expanded suite of tests to measure and compare the vector competencies of both an established colony of A. aegypti (Orlando strain [ORL]) and a field-derived colony from Collier County, FL, in 2018 (COL) for a Haitian DENV-4 human field isolate and a DENV-4 laboratory strain (Philippines H241). We immediately noted that ORL saliva positivity was higher for the field than for laboratory DENV-4 strains. In a subsequent comparison with the recent COL mosquito colony, we also observed significantly higher midgut infection of COL and ORL by the Haitian DENV-4 field strain and a significantly higher saliva positivity rate for COL, although overall saliva virus titers were similar between the two. These data point to a potential midgut infection barrier for the DENV-4 laboratory strain for both mosquito colonies and indicate that the marked differences in transmission potential estimates hinge on virus-vector combinations. Our study highlights the importance of leveraging an expanded suite of testing methods with emphasis on utilizing local mosquito populations and field-relevant dengue virus serotypes and strains to accurately estimate transmission risk in a given setting.
    MeSH term(s) Aedes/virology ; Animals ; Dengue/transmission ; Dengue/virology ; Dengue Virus/classification ; Dengue Virus/pathogenicity ; Female ; Florida ; Haiti ; Humans ; Infectious Disease Transmission, Vertical ; Mosquito Vectors/virology ; Saliva/virology ; Serogroup ; Viral Load
    Language English
    Publishing date 2021-07-07
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.00271-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: SARS-CoV-2 viroporin encoded by ORF3a triggers the NLRP3 inflammatory pathway.

    Xu, Huanzhou / Akinyemi, Ibukun A / Chitre, Siddhi A / Loeb, Julia C / Lednicky, John A / McIntosh, Michael T / Bhaduri-McIntosh, Sumita

    Virology

    2022  Volume 568, Page(s) 13–22

    Abstract: Heightened inflammatory response is a prominent feature of severe COVID-19 disease. We report that the SARS-CoV-2 ORF3a viroporin activates the NLRP3 inflammasome, the most promiscuous of known inflammasomes. Ectopically expressed ORF3a triggers IL-1β ... ...

    Abstract Heightened inflammatory response is a prominent feature of severe COVID-19 disease. We report that the SARS-CoV-2 ORF3a viroporin activates the NLRP3 inflammasome, the most promiscuous of known inflammasomes. Ectopically expressed ORF3a triggers IL-1β expression via NFκB, thus priming the inflammasome. ORF3a also activates the NLRP3 inflammasome but not NLRP1 or NLRC4, resulting in maturation of IL-1β and cleavage/activation of Gasdermin. Notably, ORF3a activates the NLRP3 inflammasome via both ASC-dependent and -independent modes. This inflammasome activation requires efflux of potassium ions and oligomerization between the kinase NEK7 and NLRP3. Importantly, infection of epithelial cells with SARS-CoV-2 similarly activates the NLRP3 inflammasome. With the NLRP3 inhibitor MCC950 and select FDA-approved oral drugs able to block ORF3a-mediated inflammasome activation, as well as key ORF3a amino acid residues needed for virus release and inflammasome activation conserved in the new variants of SARS-CoV-2 isolates across continents, ORF3a and NLRP3 present prime targets for intervention.
    MeSH term(s) Amino Acid Sequence ; Antiviral Agents/pharmacology ; COVID-19/metabolism ; COVID-19/virology ; Cell Death ; Cell Line ; Host-Pathogen Interactions ; Humans ; Inflammasomes/metabolism ; Models, Biological ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Open Reading Frames ; Potassium/metabolism ; SARS-CoV-2/physiology ; Signal Transduction/drug effects ; Viroporin Proteins/chemistry ; Viroporin Proteins/genetics ; Viroporin Proteins/metabolism
    Chemical Substances Antiviral Agents ; Inflammasomes ; NLR Family, Pyrin Domain-Containing 3 Protein ; Viroporin Proteins ; Potassium (RWP5GA015D)
    Language English
    Publishing date 2022-01-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 200425-2
    ISSN 1096-0341 ; 0042-6822
    ISSN (online) 1096-0341
    ISSN 0042-6822
    DOI 10.1016/j.virol.2022.01.003
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.172: Show issues Location:
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