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  1. Book ; Online: Chapter Using diver-operated stereo-video to monitor juvenile fish assemblages in Mediterranean coastal habitats formed by macrophytes

    Disdier-Gomez, José M / Castro-Fernández, Julia / Reñones, Olga / Moranta, Joan / Castejón, Inés / Terrados, Jorge / Hinz, Hilmar

    (Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques)

    2022  

    Series title Monitoring of Mediterranean Coastal Areas: Problems and Measurement Techniques
    Keywords The environment ; Habitat complexity ; Recruitment ; Stereo-video ; Nursery ; Coastal ecosystems ; Ecosystem services
    Language English
    Size 1 electronic resource (10 pages)
    Publisher Firenze University Press
    Publishing place Florence
    Document type Book ; Online
    Note English
    HBZ-ID HT030376008
    ISBN 9791221500301
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Lipid-specific immunoglobulin M bands in cerebrospinal fluid are associated with a reduced risk of developing progressive multifocal leukoencephalopathy during treatment with natalizumab.

    Villar, Luisa M / Costa-Frossard, Lucienne / Masterman, Thomas / Fernandez, Oscar / Montalban, Xavier / Casanova, Bonaventura / Izquierdo, Guillermo / Coret, Francisco / Tumani, Hayrettin / Saiz, Albert / Arroyo, Rafael / Fink, Katharina / Leyva, Laura / Espejo, Carmen / Simó-Castelló, María / García-Sánchez, María I / Lauda, Florian / Llufriú, Sara / Álvarez-Lafuente, Roberto /
    Olascoaga, Javier / Prada, Alvaro / Oterino, Agustín / de Andrés, Clara / Tintoré, Mar / Ramió-Torrentà, Lluis / Rodríguez-Martín, Eulalia / Picón, Carmen / Comabella, Manuel / Quintana, Ester / Agüera, Eduardo / Díaz, Santiago / Fernandez-Bolaños, Ricardo / García-Merino, Juan A / Landete, Lamberto / Menéndez-González, Manuel / Navarro, Laura / Pérez, Domingo / Sánchez-López, Fernando / Serrano-Castro, Pedro J / Tuñón, Alberto / Espiño, Mercedes / Muriel, Alfonso / Bar-Or, Amit / Álvarez-Cermeño, José C

    Annals of neurology

    2015  Volume 77, Issue 3, Page(s) 447–457

    Abstract: ... to immunosuppressants. We explored whether the presence of lipid-specific immunoglobulin M oligoclonal bands ...

    Abstract Objective: Progressive multifocal leukoencephalopathy (PML) is a serious side effect associated with natalizumab treatment in multiple sclerosis (MS). PML risk increases in individuals seropositive for anti-John Cunningham virus (JC) antibodies, with prolonged duration of natalizumab treatment, and with prior exposure to immunosuppressants. We explored whether the presence of lipid-specific immunoglobulin M oligoclonal bands in cerebrospinal fluid (CSF; IgM bands), a recognized marker of highly inflammatory MS, may identify individuals better able to counteract the potential immunosuppressive effect of natalizumab and hence be associated with a reduced risk of developing PML.
    Methods: We studied 24 MS patients who developed PML and another 343 who did not suffer this opportunistic infection during natalizumab treatment. Patients were recruited at 25 university hospitals. IgM bands were studied by isoelectric focusing and immunodetection. CSF lymphocyte counts were explored in 151 MS patients recruited at Ramon y Cajal Hospital in Madrid, Spain.
    Results: IgM bands were independently associated with decreased PML risk (odds ratio [OR] = 45.9, 95% confidence interval [CI] = 5.9-339.3, p < 0.0001) in patients treated with natalizumab. They were also associated with significantly higher CSF CD4, CD8, and B-cell numbers. Patients positive for IgM bands and anti-JC antibodies had similar levels of reduced PML risk to those who were anti-JC negative (OR = 1.55, 95% CI = 0.09-25.2, p = 1.0). Higher risk was observed in patients positive for anti-JC antibodies and negative for IgM bands (19% of the total cohort, OR = 59.71, 95% CI = 13.6-262.2).
    Interpretation: The presence of IgM bands reflects a process that may diminish the risk of PML by counteracting the excess of immunosuppression that may occur during natalizumab therapy.
    MeSH term(s) Adult ; Antibodies, Monoclonal, Humanized/adverse effects ; Biomarkers/cerebrospinal fluid ; Female ; Humans ; JC Virus/immunology ; Leukoencephalopathy, Progressive Multifocal/cerebrospinal fluid ; Leukoencephalopathy, Progressive Multifocal/chemically induced ; Male ; Middle Aged ; Multiple Sclerosis/cerebrospinal fluid ; Multiple Sclerosis/drug therapy ; Natalizumab ; Oligoclonal Bands/cerebrospinal fluid ; Risk
    Chemical Substances Antibodies, Monoclonal, Humanized ; Biomarkers ; Natalizumab ; Oligoclonal Bands
    Language English
    Publishing date 2015-03
    Publishing country United States
    Document type Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 80362-5
    ISSN 1531-8249 ; 0364-5134
    ISSN (online) 1531-8249
    ISSN 0364-5134
    DOI 10.1002/ana.24345
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Photoelectrochemical glycerol oxidation on Mo-BiVO

    Bora, Debajeet K / Nadjafi, Manouchehr / Armutlulu, Andac / Hosseini, Davood / Castro-Fernández, Pedro / Toth, Rita

    Energy advances

    2022  Volume 1, Issue 10, Page(s) 715–728

    Abstract: ... Mo-doped ... ...

    Abstract Mo-doped BiVO
    Language English
    Publishing date 2022-08-19
    Publishing country England
    Document type Journal Article
    ISSN 2753-1457
    ISSN (online) 2753-1457
    DOI 10.1039/d2ya00077f
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Thermal hydrolysis pre-treatment has no positive influence on volatile fatty acids production from sewage sludge

    Castro-Fernandez, Ander / Taboada-Santos, Anton / Balboa, Sabela / Lema, Juan M.

    Bioresource Technology. 2023 May, v. 376 p.128839-

    2023  

    Abstract: The study compares the potential to produce volatile fatty acids (VFA) from sewage sludge, both raw and thermally pre-treated in two modes of operation. In batch mode, raw sludge at pH 8 obtained the highest maximum VFA yield (0.41 g COD-VFA/g CODfed) ... ...

    Abstract The study compares the potential to produce volatile fatty acids (VFA) from sewage sludge, both raw and thermally pre-treated in two modes of operation. In batch mode, raw sludge at pH 8 obtained the highest maximum VFA yield (0.41 g COD-VFA/g CODfed) whereas pre-treated sludge achieved a lower value (0.27 g COD-VFA/g CODfed). The operation of 5-L continuous reactors showed that thermal hydrolysis pre-treatment (THP) did not have any significant influence on VFA yields, averaging 15.1 % g COD-VFA/g COD with raw sludge and 16.6 % g COD-VFA/g COD with pre-treated one. Microbial community analysis showed that phylum Firmicutes was predominant in both reactors and that the enzymatic profiles involved in VFA production were very similar regardless of the substrate fed.
    Keywords Firmicutes ; microbial communities ; pH ; sewage sludge ; technology ; thermal hydrolysis ; Volatile fatty acids ; Thermal hydrolysis pre-treatment ; Microbiology analysis
    Language English
    Dates of publication 2023-05
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2023.128839
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Thermal hydrolysis pre-treatment has no positive influence on volatile fatty acids production from sewage sludge.

    Castro-Fernandez, Ander / Taboada-Santos, Anton / Balboa, Sabela / Lema, Juan M

    Bioresource technology

    2023  Volume 376, Page(s) 128839

    Abstract: The study compares the potential to produce volatile fatty acids (VFA) from sewage sludge, both raw and thermally pre-treated in two modes of operation. In batch mode, raw sludge at pH 8 obtained the highest maximum VFA yield (0.41 g COD-VFA/g CODfed) ... ...

    Abstract The study compares the potential to produce volatile fatty acids (VFA) from sewage sludge, both raw and thermally pre-treated in two modes of operation. In batch mode, raw sludge at pH 8 obtained the highest maximum VFA yield (0.41 g COD-VFA/g CODfed) whereas pre-treated sludge achieved a lower value (0.27 g COD-VFA/g CODfed). The operation of 5-L continuous reactors showed that thermal hydrolysis pre-treatment (THP) did not have any significant influence on VFA yields, averaging 15.1 % g COD-VFA/g COD with raw sludge and 16.6 % g COD-VFA/g COD with pre-treated one. Microbial community analysis showed that phylum Firmicutes was predominant in both reactors and that the enzymatic profiles involved in VFA production were very similar regardless of the substrate fed.
    MeSH term(s) Sewage ; Fermentation ; Hydrolysis ; Microbiota ; Fatty Acids, Volatile ; Hydrogen-Ion Concentration ; Bioreactors
    Chemical Substances Sewage ; Fatty Acids, Volatile
    Language English
    Publishing date 2023-03-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 1065195-0
    ISSN 1873-2976 ; 0960-8524
    ISSN (online) 1873-2976
    ISSN 0960-8524
    DOI 10.1016/j.biortech.2023.128839
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Familia española portadora de una mutación en heterocigosis compuesta en el gen SPG7: de la incertidumbre a la realidad clínica.

    Fernández-Moreno, M C / Castro-Fernández, C / Viloria-Peñas, M M / Castilla-Guerra, L

    Neurologia (Barcelona, Spain)

    2020  Volume 35, Issue 9, Page(s) 694–696

    Title translation A Spanish family with a compound heterozygous mutation in SPG7: From uncertainty to clinical reality.
    MeSH term(s) ATPases Associated with Diverse Cellular Activities/genetics ; Humans ; Metalloendopeptidases/genetics ; Mutation ; Spastic Paraplegia, Hereditary ; Uncertainty
    Chemical Substances Metalloendopeptidases (EC 3.4.24.-) ; SPG7 protein, human (EC 3.4.24.-) ; ATPases Associated with Diverse Cellular Activities (EC 3.6.4.-)
    Language Spanish
    Publishing date 2020-03-20
    Publishing country Spain
    Document type Letter
    ZDB-ID 2654369-2
    ISSN 2173-5808 ; 2173-5808
    ISSN (online) 2173-5808
    ISSN 2173-5808
    DOI 10.1016/j.nrl.2020.01.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Vedolizumab-induced cholestatic liver injury.

    Rojas-Feria, María / Rodríguez-Fernández, Miguel / Castro-Fernández, Manuel / Suárez-García, Emilio

    Gastroenterologia y hepatologia

    2020  Volume 45, Issue 1, Page(s) 64–65

    MeSH term(s) Aged, 80 and over ; Anti-Inflammatory Agents/administration & dosage ; Antibodies, Monoclonal, Humanized/adverse effects ; Chemical and Drug Induced Liver Injury/diagnosis ; Chemical and Drug Induced Liver Injury/drug therapy ; Chemical and Drug Induced Liver Injury/etiology ; Cholestasis, Intrahepatic/chemically induced ; Cholestasis, Intrahepatic/diagnosis ; Cholestasis, Intrahepatic/drug therapy ; Colitis, Ulcerative/drug therapy ; Gastrointestinal Agents/administration & dosage ; Gastrointestinal Agents/adverse effects ; Humans ; Maintenance Chemotherapy ; Male ; Prednisone/administration & dosage
    Chemical Substances Anti-Inflammatory Agents ; Antibodies, Monoclonal, Humanized ; Gastrointestinal Agents ; vedolizumab (9RV78Q2002) ; Prednisone (VB0R961HZT)
    Language Spanish
    Publishing date 2020-11-25
    Publishing country Spain
    Document type Case Reports ; Letter
    ZDB-ID 632502-6
    ISSN 0210-5705
    ISSN 0210-5705
    DOI 10.1016/j.gastrohep.2020.11.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Structure of an ancestral ADP-dependent kinase with fructose-6P reveals key residues for binding, catalysis, and ligand-induced conformational changes.

    Muñoz, Sebastián M / Castro-Fernandez, Victor / Guixé, Victoria

    The Journal of biological chemistry

    2020  Volume 296, Page(s) 100219

    Abstract: ADP-dependent kinases were first described in archaea, although their presence has also been reported in bacteria and eukaryotes (human and mouse). This enzyme family comprises three substrate specificities; specific phosphofructokinases (ADP-PFKs), ... ...

    Abstract ADP-dependent kinases were first described in archaea, although their presence has also been reported in bacteria and eukaryotes (human and mouse). This enzyme family comprises three substrate specificities; specific phosphofructokinases (ADP-PFKs), specific glucokinases (ADP-GKs), and bifunctional enzymes (ADP-PFK/GK). Although many structures are available for members of this family, none exhibits fructose-6-phosphate (F6P) at the active site. Using an ancestral enzyme, we obtain the first structure of an ADP-dependent kinase (AncMsPFK) with F6P at its active site. Key residues for sugar binding and catalysis were identified by alanine scanning, D36 being a critical residue for F6P binding and catalysis. However, this residue hinders glucose binding because its mutation to alanine converts the AncMsPFK enzyme into a specific ADP-GK. Residue K179 is critical for F6P binding, while residues N181 and R212 are also important for this sugar binding, but to a lesser extent. This structure also provides evidence for the requirement of both substrates (sugar and nucleotide) to accomplish the conformational change leading to a closed conformation. This suggests that AncMsPFK mainly populates two states (open and closed) during the catalytic cycle, as reported for specific ADP-PFK. This situation differs from that described for specific ADP-GK enzymes, where each substrate independently causes a sequential domain closure, resulting in three conformational states (open, semiclosed, and closed).
    MeSH term(s) Amino Acid Sequence ; Archaeal Proteins/chemistry ; Archaeal Proteins/genetics ; Archaeal Proteins/metabolism ; Binding Sites ; Biocatalysis ; Cloning, Molecular ; Crystallography, X-Ray ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Fructosephosphates/chemistry ; Fructosephosphates/metabolism ; Gene Expression ; Genetic Vectors/chemistry ; Genetic Vectors/metabolism ; Glucokinase/chemistry ; Glucokinase/genetics ; Glucokinase/metabolism ; Kinetics ; Ligands ; Methanosarcinales/chemistry ; Methanosarcinales/enzymology ; Methanosarcinales/genetics ; Models, Molecular ; Phosphofructokinases/chemistry ; Phosphofructokinases/genetics ; Phosphofructokinases/metabolism ; Phosphotransferases (Alcohol Group Acceptor)/chemistry ; Phosphotransferases (Alcohol Group Acceptor)/genetics ; Phosphotransferases (Alcohol Group Acceptor)/metabolism ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Protein Interaction Domains and Motifs ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Sequence Alignment ; Sequence Homology, Amino Acid ; Substrate Specificity
    Chemical Substances Archaeal Proteins ; Fructosephosphates ; Ligands ; Recombinant Proteins ; fructose-6-phosphate (6814-87-5) ; Phosphofructokinases (EC 2.7.1 -) ; Phosphotransferases (Alcohol Group Acceptor) (EC 2.7.1.-) ; ADP D-fructose-6-phosphate 1-phosphotransferase (EC 2.7.1.146) ; Glucokinase (EC 2.7.1.2)
    Language English
    Publishing date 2020-12-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1074/jbc.RA120.015376
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Pediatric Ventilation Skills by Non-Healthcare Students: Effectiveness, Self-Perception, and Preference.

    Martínez-Isasi, Santiago / Jorge-Soto, Cristina / Castro-Fernández, Cecilia / Baltar-Lorenzo, Clara / Sobrido-Prieto, María / Manteiga-Urbón, Jose / Barcala-Furelos, Roberto

    International journal of environmental research and public health

    2023  Volume 20, Issue 4

    Abstract: Since a great number of infant cardiopulmonary arrests occur outside of the hospital, it is crucial to train laypersons in cardiopulmonary resuscitation techniques, especially those professionals that will work with infants and children. The main ... ...

    Abstract Since a great number of infant cardiopulmonary arrests occur outside of the hospital, it is crucial to train laypersons in cardiopulmonary resuscitation techniques, especially those professionals that will work with infants and children. The main objective of this study was to evaluate the efectiveness of ventilations performed by professional training students. The secondary objective was to analyze the preference between different ventilation and chest-compression methods. The sample consisted of 32 professional training students, 15 preschool students, and 17 physical education students. The activity was conducted separately for each group, and we provided a 10 min theoretical training about infant basic life support followed by a 45 min practical training using a Laerdal Little Anne QCPR CPR manikin. A practical test in pairs was organized to record the ventilation as performed by the participants, establishing the difference between the efficacious and the non-efficacious ones. Furthermore, we handed out a survey before and after training to evaluate their knowledge. More than 90% of the students completely agreed with the importance of learning cardiopulmonary resuscitation techniques for their professional future. More than half of the sample considered that they perform the rescue breathings with the mouth-to-mouth method better. We observed that through mouth-to-mouth-nose ventilations, the number of effective ventilations was significantly higher than the effective ventilations provided by a self-inflating bag and mask (EffectiveMtoMN 6.42 ± 4.27 vs. EffectiveMask 4.75 ± 3.63 (
    MeSH term(s) Infant ; Child, Preschool ; Humans ; Child ; Cardiopulmonary Resuscitation/education ; Respiration ; Heart Arrest ; Students ; Self Concept
    Language English
    Publishing date 2023-02-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175195-X
    ISSN 1660-4601 ; 1661-7827
    ISSN (online) 1660-4601
    ISSN 1661-7827
    DOI 10.3390/ijerph20043026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Characterisation of kinetics, substrate inhibition and product activation by AMP of bifunctional ADP-dependent glucokinase/phosphofructokinase from Methanococcus maripaludis.

    Vallejos-Baccelliere, Gabriel / Kaufman, Sergio B / González-Lebrero, Rodolfo M / Castro-Fernandez, Víctor / Guixé, Victoria

    The FEBS journal

    2022  Volume 289, Issue 23, Page(s) 7519–7536

    Abstract: Methanogenic archaea have received attention due to their potential use in biotechnological applications such as methane production, so their metabolism and regulation are topics of special interest. When growing in a nutrient-rich medium, these ... ...

    Abstract Methanogenic archaea have received attention due to their potential use in biotechnological applications such as methane production, so their metabolism and regulation are topics of special interest. When growing in a nutrient-rich medium, these organisms exhibit gluconeogenic metabolism; however, under starvation conditions, they turn to glycolytic metabolism. To date, no regulatory mechanism has been described for this gluconeogenic/glycolytic metabolic switch. Here, we report that adenosine monophosphate (AMP) activates both enzymatic activities of the bifunctional adenosine diphosphate (ADP)-dependent phosphofructokinase/glucokinase from Methanococcus maripaludis (MmPFK/GK). To understand this phenomenon, we performed a comprehensive kinetic characterisation, including determination of the kinetics, substrate inhibition and AMP activation mechanism of this enzyme. We determined that MmPFK/GK has an ordered-sequential mechanism, in which MgADP is the first substrate to bind and AMP is the last product released. The enzyme also displays substrate inhibition by both sugar substrates; we determined that this inhibition occurs through the formation of catalytically nonproductive enzyme complexes caused by sugar binding. For both activities, the AMP activation mechanism occurs primarily through incremental changes in the affinity for the sugar substrate, with this effect being higher in the GK than in the PFK activity. Interestingly, due to the increase in the sugar substrate affinity caused by AMP, an enhancement in the sugar substrate inhibition effect was also observed for both activities, which can be explained by an increase in sugar binding leading to the formation of dead-end complexes. These results shed light on the regulatory mechanisms of methanogenic archaeal sugar metabolism, a phenomenon that has been largely unexplored.
    MeSH term(s) Phosphofructokinases ; Adenosine Diphosphate ; Adenosine Monophosphate ; Methanococcus/genetics ; Sugars
    Chemical Substances Phosphofructokinases (EC 2.7.1 -) ; Adenosine Diphosphate (61D2G4IYVH) ; Adenosine Monophosphate (415SHH325A) ; ADP-dependent glucokinase (EC 2.7.1.-) ; Sugars
    Language English
    Publishing date 2022-06-29
    Publishing country England
    Document type Journal Article
    ZDB-ID 2173655-8
    ISSN 1742-4658 ; 1742-464X
    ISSN (online) 1742-4658
    ISSN 1742-464X
    DOI 10.1111/febs.16557
    Database MEDical Literature Analysis and Retrieval System OnLINE

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