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Article ; Online: Title: Repeated implantation failure is associated with increased Th17/Treg cell ratio, during the secretory phase of the human endometrium.

Berdiaki, Aikaterini / Vergadi, Eleni / Makrygiannakis, Fanourios / Vrekoussis, Thomas / Makrigiannakis, Antonios

2023 Volume 161, Page(s) 104170

Abstract: Repeated implantation failure (RIF) is a significant limiting factor in assisted reproduction. Chronic endometrial inflammation has been noted in RIF women, therefore we sought to investigate the potential association of endometrial Th17/Treg ratio and ... ...

Abstract	Repeated implantation failure (RIF) is a significant limiting factor in assisted reproduction. Chronic endometrial inflammation has been noted in RIF women, therefore we sought to investigate the potential association of endometrial Th17/Treg ratio and endometrial inflammation in these cases. Endometrial pipelle biopsies were obtained from volunteers, 29 women with RIF (failure to achieve pregnancy following at least 3 transfers of high-grade embryos in IVF-cycles) and 27 fertile women (at least one child) in total, at the secretory phase of the menstrual cycle. Using tissues from 17 fertile and 18 RIF endometrial samples, stromal and immune cells were isolated and flow cytometry analysis was performed to determine Th17 and CD4+ CD25
MeSH term(s)	Pregnancy ; Child ; Humans ; Female ; Embryo Implantation ; T-Lymphocytes, Regulatory ; Nuclear Receptor Subfamily 1, Group F, Member 3/genetics ; Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism ; Endometrium ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/metabolism ; Inflammation/metabolism ; RNA, Messenger/metabolism ; Inflammation Mediators/metabolism
Chemical Substances	Nuclear Receptor Subfamily 1, Group F, Member 3 ; Forkhead Transcription Factors ; RNA, Messenger ; Inflammation Mediators
Language	English
Publishing date	2023-11-19
Publishing country	Ireland
Document type	Journal Article
ZDB-ID	424421-7
ISSN	1872-7603 ; 0165-0378
ISSN (online)	1872-7603
ISSN	0165-0378
DOI	10.1016/j.jri.2023.104170
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Article: Challenges in the Diagnosis of Viral Encephalitis in Children: The Case of Two Siblings.

Vergadi, Eleni / Zacharioudaki, Maria / Raissaki, Maria / Galanakis, Emmanouil

Infectious disease reports

2022 Volume 14, Issue 1, Page(s) 106–111

Abstract: Encephalitis in children may lead to adverse outcomes and long-term neurodevelopmental sequelae. The prompt identification of the causative agent is important to guide proper management in cases with encephalitis; however, the etiology often remains ... ...

Abstract	Encephalitis in children may lead to adverse outcomes and long-term neurodevelopmental sequelae. The prompt identification of the causative agent is important to guide proper management in cases with encephalitis; however, the etiology often remains undetermined. The use of polymerase chain reaction (PCR) analysis in the cerebrospinal fluid (CSF) has increased the diagnostic yield in encephalitis cases; however, it may be occasionally misleading. In this article, we describe the case of a male immunocompetent child with encephalitis in which human herpesvirus-7 (HHV-7) was detected in CSF by PCR. As the detection of HHV-7 DNA in the CSF alone is insufficient to prove an etiologic association of severe encephalitis in immunocompetent children, alternative diagnoses were pursued. Enterovirus (E-11) was detected by PCR analysis of the nasopharyngeal and rectal swabs of the male patient. The final diagnosis was facilitated by the findings in his sibling, which presented concurrently with enteroviral encephalitis. Failure to detect enterovirus in the CSF by PCR does not exclude enteroviral encephalitis; screening of other samples, from other body sites, may be necessary to identify the virus, and physicians should take into consideration all evidence, including history, clinical presentation, and sick contacts' clinical status.
Language	English
Publishing date	2022-02-11
Publishing country	Switzerland
Document type	Case Reports
ISSN	2036-7430
ISSN	2036-7430
DOI	10.3390/idr14010014
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Article ; Online: Mild coronary artery dilatation developed in some children with mild COVID-19 but completely regressed within 3 months.

Rouva, Glykeria / Vergadi, Eleni / Hatzidaki, Eleftheria / Germanakis, Ioannis

Acta paediatrica (Oslo, Norway : 1992)

2022 Volume 111, Issue 9, Page(s) 1764–1770

Abstract: Aim: We studied the incidence and time course of any coronary artery changes in children up to 2 years of age who were hospitalised with mild COVID-19.: Methods: This was a single-centre prospective study of 29 children (19 males) with a median age ... ...

Abstract	Aim: We studied the incidence and time course of any coronary artery changes in children up to 2 years of age who were hospitalised with mild COVID-19. Methods: This was a single-centre prospective study of 29 children (19 males) with a median age of 3 months and interquartile range (IQR) of 1.6-4.3 months. They were admitted to a Greek University hospital for mild COVID-19 from 1 March to 30 December 2021. Three echocardiographic evaluations were performed at a median (IQR) of 19 (16-24) days, 82 (75-89) days and 172 (163-197) after the first symptoms. The prevalence of coronary artery dilation, regression, and changes was documented. Results: Coronary artery dilation was present in 3 (10.3%) cases at the first evaluation, with complete regression at the second. Regression was observed in 18/24 (75%) cases with follow-up data and 9 (31%) demonstrated significant z-score changes of >2. Coronary artery changes in any segment at any time were documented in 18/29 (62%) of the patients. Conclusion: Cases of transient and very mild coronary artery dilatation following mild COVID-19 completely regressed within 3 months. Large-scale studies are needed to document the extent and time course of coronary artery dilation following paediatric COVID-19.
MeSH term(s)	COVID-19 ; Child ; Coronary Aneurysm/etiology ; Coronary Vessels ; Dilatation/adverse effects ; Humans ; Infant ; Male ; Mucocutaneous Lymph Node Syndrome/epidemiology ; Prospective Studies ; Retrospective Studies
Language	English
Publishing date	2022-07-04
Publishing country	Norway
Document type	Journal Article
ZDB-ID	203487-6
ISSN	1651-2227 ; 0365-1436 ; 0803-5253
ISSN (online)	1651-2227
ISSN	0365-1436 ; 0803-5253
DOI	10.1111/apa.16465
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Um IV Zs.95: Show issues

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Article ; Online: Attitudes of junior healthcare professionals towards mandatory vaccination.

Kakatsaki, Ioanna / Vergadi, Eleni / Paraskakis, Emmanouil / Galanakis, Emmanouil

Vaccine

2022 Volume 40, Issue 15, Page(s) 2247–2250

Abstract: Vaccination of healthcare professionals (HCPs) is a key measure to prevent infections in healthcare facilities, but uptake rates often remain low. Mandatory vaccination policies have been occasionally implemented to increase compliance among HCPs, but ... ...

Abstract	Vaccination of healthcare professionals (HCPs) is a key measure to prevent infections in healthcare facilities, but uptake rates often remain low. Mandatory vaccination policies have been occasionally implemented to increase compliance among HCPs, but this remains an issue of controversy. The purpose of this survey was to assess the attitudes and beliefs of trainee HCPs towards mandatory occupational vaccination and further explore the factors that determine their decision. In this cross-sectional survey, trainees consisted of medical residents and medical and nursing students undergoing their clinical clerkship. An anonymous questionnaire was distributed following pilot testing. In total, 410 trainees participated (response rate: 90.1%), of whom 194 (47.3%) were residents, 154 (37.6%) medical and 62 (15.1%) nursing students. Most participants (320/410, 78%) supported mandatory occupational vaccination, stating that it should be applied to promote public welfare and benefit (294/320, 91.9%) or should be a prerequisite for employment (271/320, 84.7%). Only 22/410 (5.4%) of HCPs opposed to mandatory occupational vaccination. The primary reasons for a negative attitude were the belief that personal rights outweigh public benefit (10/22, 45.5%) and the fear of side effects (9/22, 40.9%). Univariate analysis revealed that HCPs that have been informed by vaccination campaigns or had higher knowledge scores, were more likely to support mandatory occupational vaccination (OR 1.7, 95% CI: 1.1-2.7, p 0.038 and OR 1.7, 95% CI: 1.02- 2.7. p 0.044, respectively). In conclusion, most medical and nursing trainees in this study supported mandatory vaccination for HCPs. Focusing on continuing professional education on vaccines is important to positively determine HCPs' attitudes towards occupational vaccination and increase their vaccination uptake rates.
MeSH term(s)	Attitude of Health Personnel ; Cross-Sectional Studies ; Health Knowledge, Attitudes, Practice ; Health Personnel ; Humans ; Influenza Vaccines ; Surveys and Questionnaires ; Vaccination
Chemical Substances	Influenza Vaccines
Language	English
Publishing date	2022-03-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2022.03.010
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

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Article ; Online: Infectious Diseases Associated with Desert Dust Outbreaks: A Systematic Review.

Vergadi, Eleni / Rouva, Glykeria / Angeli, Maria / Galanakis, Emmanouil

International journal of environmental research and public health

2022 Volume 19, Issue 11

Abstract: Background: Desert dust outbreaks and dust storms are the major source of particulate matter globally and pose a major threat to human health. We investigated the microorganisms transported with desert dust particles and evaluated their potential impact ...

Abstract	Background: Desert dust outbreaks and dust storms are the major source of particulate matter globally and pose a major threat to human health. We investigated the microorganisms transported with desert dust particles and evaluated their potential impact on human health. Methods: A systematic review of all reports on the association between non-anthropogenic desert dust pollution, dust microorganisms and human health is conducted. Results: In total, 51 articles were included in this review. The affected regions studied were Asia (32/51, 62.7%) followed by Europe (9/51, 17.6%), America (6/51, 11.8%), Africa (4/51, 7.8%) and Australia (1/51, 2.0%). The Sahara Desert was the most frequent source of dust, followed by Asian and American deserts. In 39/51 studies the dust-related microbiome was analyzed, while, in 12/51 reports, the association of desert dust with infectious disease outbreaks was examined. Pathogenic and opportunistic agents were isolated from dust in 24/39 (61.5%) and 29/39 (74.4%) of the studies, respectively. A significant association of dust events with infectious disease outbreaks was found in 10/12 (83.3%) reports. The infectious diseases that were mostly investigated with dust outbreaks were pneumonia, respiratory tract infections, COVID-19, pulmonary tuberculosis and coccidioidomycosis. Conclusions: Desert dust outbreaks are vehicles of a significant number of pathogenic or opportunistic microorganisms and limited data indicate an association between dust events and infectious disease outbreaks. Further research is required to strengthen the correlation between dust events and infectious diseases and subsequently guide preventive public health measures.
MeSH term(s)	Air Pollutants/analysis ; Air Pollution/analysis ; COVID-19 ; Communicable Diseases/epidemiology ; Desert Climate ; Disease Outbreaks ; Dust/analysis ; Humans ; Particulate Matter
Chemical Substances	Air Pollutants ; Dust ; Particulate Matter
Language	English
Publishing date	2022-06-05
Publishing country	Switzerland
Document type	Journal Article ; Review ; Systematic Review
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph19116907
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Article ; Online: Abnormal gait and hypoglycorrhachia in a toddler with seizures.

Angeli, Maria / Vergadi, Eleni / Niotakis, Georgios / Raissaki, Maria / Galanakis, Emmanouil

Pediatric investigation

2022 Volume 6, Issue 1, Page(s) 47–49

Abstract: Introduction: Glucose transporter type 1 (Glut1) deficiency syndrome is a treatable neurometabolic disorder characterized by seizures, developmental delay, and hypoglycorrhachia. Due to the rareness and non-specific clinical manifestations, it is ... ...

Abstract	Introduction: Glucose transporter type 1 (Glut1) deficiency syndrome is a treatable neurometabolic disorder characterized by seizures, developmental delay, and hypoglycorrhachia. Due to the rareness and non-specific clinical manifestations, it is usually mis- or underdiagnosed. Case presentation: We report the case of a toddler who presented with afebrile epileptic seizures and abnormal gait. Brain imaging and electroencephalogram were normal. Further investigation of the cerebrospinal fluid revealed hypoglycorrhachia that was the clue to the diagnosis of Glut1 deficiency syndrome and the initiation of treatment with ketogenic diet. Conclusion: Our case highlights the importance of lumbar puncture while investigating a child with epileptic seizures and abnormal gait or developmental delay, in order not to miss treatable neurometabolic conditions, such as Glut1 deficiency syndrome.
Language	English
Publishing date	2022-02-21
Publishing country	United States
Document type	Case Reports
ISSN	2574-2272
ISSN (online)	2574-2272
DOI	10.1002/ped4.12311
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Article ; Online: Challenges in the Diagnosis of Viral Encephalitis in Children

Eleni Vergadi / Maria Zacharioudaki / Maria Raissaki / Emmanouil Galanakis

Infectious Disease Reports, Vol 14, Iss 14, Pp 106-

The Case of Two Siblings

2022 Volume 111

Abstract	Encephalitis in children may lead to adverse outcomes and long-term neurodevelopmental sequelae. The prompt identification of the causative agent is important to guide proper management in cases with encephalitis; however, the etiology often remains undetermined. The use of polymerase chain reaction (PCR) analysis in the cerebrospinal fluid (CSF) has increased the diagnostic yield in encephalitis cases; however, it may be occasionally misleading. In this article, we describe the case of a male immunocompetent child with encephalitis in which human herpesvirus-7 (HHV-7) was detected in CSF by PCR. As the detection of HHV-7 DNA in the CSF alone is insufficient to prove an etiologic association of severe encephalitis in immunocompetent children, alternative diagnoses were pursued. Enterovirus (E-11) was detected by PCR analysis of the nasopharyngeal and rectal swabs of the male patient. The final diagnosis was facilitated by the findings in his sibling, which presented concurrently with enteroviral encephalitis. Failure to detect enterovirus in the CSF by PCR does not exclude enteroviral encephalitis; screening of other samples, from other body sites, may be necessary to identify the virus, and physicians should take into consideration all evidence, including history, clinical presentation, and sick contacts’ clinical status.
Keywords	encephalitis ; seizures ; enterovirus ; children ; neonates ; HHV-7 ; Other systems of medicine ; RZ201-999
Subject code	610
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Determinants of low uptake of vaccination against influenza, measles, and hepatitis B among healthcare professionals in Greece: a multicenter cross-sectional study.

Vrachnaki, Olga / Vergadi, Eleni / Ioannidou, Eleni / Galanakis, Emmanouil

Human vaccines & immunotherapeutics

2020 Volume 16, Issue 11, Page(s) 2663–2669

Abstract: Vaccination is recommended for healthcare professionals (HCPs) to protect them against vaccine-preventable diseases (VPDs); however, uptake rates are low. This study aimed to evaluate HCPs' influenza, hepatitis B, and measles vaccine uptake in all ... ...

Abstract	Vaccination is recommended for healthcare professionals (HCPs) to protect them against vaccine-preventable diseases (VPDs); however, uptake rates are low. This study aimed to evaluate HCPs' influenza, hepatitis B, and measles vaccine uptake in all healthcare levels in Crete, Greece. We conducted a questionnaire-based, cross-sectional multicenter study in 2018, including HCPs employed at 18 primary care centers and 3 hospitals. Overall, 2,246 HCPs responded (57.2% of the target population). The influenza vaccine uptake rate was 36.1% (810/2,246), with the annual vaccination rate at 14.8% (332/2,246) over the previous 5 years. Concurrently, the hepatitis B 3-dose vaccine uptake rate was 60.3% (1,316/2,181). Among the participating HCPs, 70.7% (1,457/2,061) had measles immunity due to previous illness (959/2,061, 46.5%), a 2-dose vaccination scheme (461/2,061, 22.4%), or serological confirmation (37/2,061, 1.8%). Vaccine uptake rates differed between groups depending on age, profession, and workplace setting. Logistic regression analysis revealed that risk factors for no influenza vaccine uptake during the previous season were younger age (≤45 years; odds ratio [OR] 1.35, 95% confidence interval [CI]: 1.08-1.66), profession other than physician (OR 2.94, 95%CI: 2.09-4.12), and working in hospitals (OR 1.39, 95%CI 1.02-1.89). Older age (>45 years) was an independent risk factor for not receiving a measles (OR 26.74, 95%CI: 17.41-41.06) or hepatitis B vaccine (OR 1.36, 95%CI 1.09-1.7). Working in primary care was an independent risk factor for not getting a hepatitis B vaccine (OR 1.52, 95%CI: 1.15-2.1). Our findings indicate that individualized and targeted interventions should be implemented to increase vaccine uptake among HCPs.
MeSH term(s)	Adult ; Cross-Sectional Studies ; Greece/epidemiology ; Health Knowledge, Attitudes, Practice ; Health Personnel ; Hepatitis B ; Humans ; Influenza Vaccines ; Influenza, Human/epidemiology ; Influenza, Human/prevention & control ; Measles/epidemiology ; Measles/prevention & control ; Middle Aged ; Surveys and Questionnaires ; Vaccination
Chemical Substances	Influenza Vaccines
Language	English
Publishing date	2020-05-13
Publishing country	United States
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2664176-8
ISSN	2164-554X ; 2164-5515
ISSN (online)	2164-554X
ISSN	2164-5515
DOI	10.1080/21645515.2020.1741311
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Zs.A 6967: Show issues

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Article ; Online: Extensive Cellulitis and Bacteremia Due to Streptococcus Pseudoporcinus in a Child With Klippel-Trenaunay Syndrome.

Vergadi, Eleni / Goniotakis, Ioannis / Maraki, Sofia / Galanakis, Emmanouil

The Pediatric infectious disease journal

2021 Volume 40, Issue 8, Page(s) e316–e318

Abstract: Streptococcus pseudoporcinus is a newly recognized β-hemolytic streptococcus, that is considered a rare pathogen in adults. Infections in children have not been reported. We describe a child with Klippel-Trenaunay syndrome that developed of S. ... ...

Abstract	Streptococcus pseudoporcinus is a newly recognized β-hemolytic streptococcus, that is considered a rare pathogen in adults. Infections in children have not been reported. We describe a child with Klippel-Trenaunay syndrome that developed of S. pseudoporcinus cellulitis and bacteremia, which was difficult-to-treat, relapsed and required prolonged antibiotic courses. S. pseudoporcinus can cause invasive infection in children, especially in the presence of predisposing conditions.
MeSH term(s)	Anti-Bacterial Agents/therapeutic use ; Bacteremia/drug therapy ; Bacteremia/etiology ; Cellulitis/drug therapy ; Cellulitis/etiology ; Child ; Humans ; Klippel-Trenaunay-Weber Syndrome/complications ; Male ; Streptococcal Infections/complications ; Streptococcal Infections/drug therapy ; Streptococcus/drug effects ; Streptococcus/isolation & purification ; Treatment Outcome
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2021-04-09
Publishing country	United States
Document type	Case Reports
ZDB-ID	392481-6
ISSN	1532-0987 ; 0891-3668
ISSN (online)	1532-0987
ISSN	0891-3668
DOI	10.1097/INF.0000000000003160
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Zs.A 1852: Show issues

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Article: Akt Inhibition Promotes Autophagy and Clearance of Group B

Pantazi, Ioanna / Papafragkos, Iosif / Kolliniati, Ourania / Lapi, Ioanna / Tsatsanis, Christos / Vergadi, Eleni

Pathogens (Basel, Switzerland)

2022 Volume 11, Issue 10

Abstract: ... Group ... ...

Abstract	Group B
Language	English
Publishing date	2022-09-30
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens11101134
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