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Article ; Online: Comparison of HPV detection rate in formalin-fixed paraffin-embedded tissues of head and neck carcinoma using two DNA extraction kits and three amplification methods.

Božić, Ljiljana / Jovanović, Tanja / Šmitran, Aleksandra / Janković, Marko / Knežević, Aleksandra

2020 Volume 128, Issue 6, Page(s) 501–507

Abstract: The potential problems of DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples and amplification efficiency of Human papilloma virus (HPV) may occur in the molecular studies of head and neck squamous carcinoma (HNSCC). The aim of ... ...

Abstract	The potential problems of DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue samples and amplification efficiency of Human papilloma virus (HPV) may occur in the molecular studies of head and neck squamous carcinoma (HNSCC). The aim of this study was to compare HPV detection rate in FFPE tissues of oral, oropharyngeal, hypopharyngeal, and laryngeal cancers using two silica-based extraction kits and three amplification methods. A total of 50 FFPE specimens from HNSCC tissues were analyzed. The quality and quantity of the extracted DNA were tested by spectrophotometry. HPV DNA was detected using a single polymerase chain reaction (PCR), a nested PCR, and a Real-time PCR kit. Statistically significantly higher DNA quality and quantity was observed using the QIAamp DNA FFPE Tissue Kit than when using the QIAamp DNA Mini Kit. There was not HPV amplification in any of the 50 FFPE samples using the single PCR and Real-time PCR kits, whereas HPV DNA was detected in 22% of samples using nested PCR. Comparing results of the three different methods showed that HPV DNA was detected only with nested PCR. The results presented imply that nested PCR is the most appropriate method for the detection of HPV DNA in FFPE samples, along with adequate DNA extraction methods.
MeSH term(s)	Alphapapillomavirus/genetics ; Carcinoma ; DNA ; Formaldehyde ; Humans ; Papillomaviridae/genetics ; Papillomavirus Infections/diagnosis ; Paraffin Embedding ; Tissue Fixation
Chemical Substances	Formaldehyde (1HG84L3525) ; DNA (9007-49-2)
Language	English
Publishing date	2020-11-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1224820-4
ISSN	1600-0722 ; 0909-8836
ISSN (online)	1600-0722
ISSN	0909-8836
DOI	10.1111/eos.12746
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Article ; Online: Biofilm production and twitching and swarming motility of clinical isolates Acinetobacter baumannii

Ranin Jovana / Šmitran Aleksandra / Gajić Ina

Medicinski Podmladak, Vol 70, Iss 1, Pp 28-

2019 Volume 33

Abstract: Introduction: Acinetobacter baumannii is a ubiquitous gram-negative bacillus that has emerged as one of the most important cause of healthcare-associated infections primarly due to multidrug resistance and its ability to survive within biofilm on ... ...

Abstract	Introduction: Acinetobacter baumannii is a ubiquitous gram-negative bacillus that has emerged as one of the most important cause of healthcare-associated infections primarly due to multidrug resistance and its ability to survive within biofilm on nonliving objects. Acinetobacter possesses pili that mediates its adherence to abiotic surfaces and certain types of motility such as twitching and swarming. Aim: the aim of this study was to determine the capacity of the biofilm production of a. baumannii and the twiching and swarming motility. Material and methods: a total of 128 strains of a. baumannii isolated from blood (34), wound swabs (35), respiratory tract (39) and other clinical materials (20) were included in the study. Biofilm-producing ability was tested in the microtiter plates at 26°C and 37°C for 24 hours incubation period and at 37°C for 48h incubation as well. The presence of the pili was determined by the testing of the twitching and swarming motility in a semi-solid nutrient medium. Results: More than 90% of isolates showed moderate or strong biofilm production capacity regardless of the experimental conditions. Decreasing in biofilm production was noticed in the group of strong biofilm producers (p < 0.001) during prolonged incubation time (48h). During the 24-hour incubation period, wound and respiratory isolates were significantly more frequent in the group of strong biofilm producers compared to blood and other isolates. No correlation between specific types of motility and biofilm production was noticed. Conclusion: the biofilm production remained stable under the tested experimental condition. There was no decisive influence of pili on this virulence factor.
Keywords	Acinetobacter baumannii ; biofilm ; motility ; pili ; Medicine ; R
Subject code	630
Language	English
Publishing date	2019-01-01T00:00:00Z
Publisher	University of Belgrade, Medical Faculty
Document type	Article ; Online
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Article: Carbapenem-Resistant

Smitran, Aleksandra / Lukovic, Bojana / Bozic, LJiljana / Jelic, Dijana / Jovicevic, Milos / Kabic, Jovana / Kekic, Dusan / Ranin, Jovana / Opavski, Natasa / Gajic, Ina

Microorganisms

2023 Volume 11, Issue 1

Abstract: This study aimed to investigate the biofilm-production ability of carbapenem-resistant Acinetobacter baumannii (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against ... ...

Abstract	This study aimed to investigate the biofilm-production ability of carbapenem-resistant Acinetobacter baumannii (CRAB), the biofilm-eradication potential of 70% ethanol and 0.5% sodium hypochlorite, the effects of selenium nanoparticles (SeNPs) against planktonic and biofilm-embedded CRAB, and the relationship between biofilm production and bacterial genotypes. A total of 111 CRAB isolates were tested for antimicrobial susceptibility, biofilm formation, presence of the genes encoding carbapenemases, and biofilm-associated virulence factors. The antibiofilm effects of disinfectants and SeNPs against CRAB isolates were also tested. The vast majority of the tested isolates were biofilm producers (91.9%). The bap, ompA, and csuE genes were found in 57%, 70%, and 76% of the CRAB isolates, with the csuE being significantly more common among biofilm producers (78.6%) compared to non-biofilm-producing CRAB (25%). The tested disinfectants showed a better antibiofilm effect on moderate and strong biofilm producers than on weak producers (p < 0.01). The SeNPs showed an inhibitory effect against all tested planktonic (MIC range: 0.00015 to >1.25 mg/mL) and biofilm-embedded CRAB, with a minimum biofilm inhibitory concentration of less than 0.15 mg/mL for 90% of biofilm producers. In conclusion, SeNPs might be used as promising therapeutic and medical device coating agents, thus serving as an alternative approach for the prevention of biofilm-related infections.
Language	English
Publishing date	2023-01-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11010171
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Article ; Online: The significance of human papillomaviruses in head and neck cancer development

Božić Ljiljana / Jovičić Sanja / Šmitran Aleksandra

Scripta Medica, Vol 47, Iss 1, Pp 69-

2016 Volume 73

Abstract: Head and neck cancers (HNC) are a heterogeneous group of diseases. Histopathological, these are head and neck squamous cell carcinoma (HNSCC) epithelium of the skin, mucosa of the upper aerodigestive tract, pharynx and larynx. HNSCC is the fifth leading ... ...

Abstract	Head and neck cancers (HNC) are a heterogeneous group of diseases. Histopathological, these are head and neck squamous cell carcinoma (HNSCC) epithelium of the skin, mucosa of the upper aerodigestive tract, pharynx and larynx. HNSCC is the fifth leading cause of cancer worldwide. The most important risk factors are alcohol and smoking. In the recent years, the studies have been conducted in order to prove the role of HPV in HNSCC carcinogenesis. The prevalence of HPV in HNSCC is 30%. HPV is often detected in biological materials of males. People with HPV positive HNSCC respond better to treatment and have a better treatment outcome than patients with no proven HPV. In the future, HPV should be the marker that will influence the choice of therapy in patients with HNSCC, but also the factor that will indicate the outcome of this malignancy in the human population.
Keywords	hpv ; head and neck cancer ; diagnosis ; Medicine ; R
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Medical Society of the Republic of Srpska, Banja Luka, University of Banja Luka. Faculty of Medicine
Document type	Article ; Online
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Article ; Online: Effects of penicillin and erythromycin on adherence of invasive and noninvasive isolates of Streptococcus pyogenes to laminin.

Šmitran, Aleksandra / Vuković, Dragana / Gajić, Ina / Marinković, Jelena / Ranin, Lazar

Memorias do Instituto Oswaldo Cruz

2015 Volume 110, Issue 5, Page(s) 684–686

Abstract: This study investigated the possible relationship between the invasiveness of group A Streptococcus (GAS) strains and their abilities to adhere to laminin and assessed the effects of subinhibitory concentrations of penicillin and erythromycin on the ... ...

Abstract	This study investigated the possible relationship between the invasiveness of group A Streptococcus (GAS) strains and their abilities to adhere to laminin and assessed the effects of subinhibitory concentrations of penicillin and erythromycin on the ability of GAS to adhere to laminin. The adherence of noninvasive and highly invasive isolates of GAS to laminin was significantly higher than the adherence displayed by isolates of low invasiveness. Antibiotic treatment caused significant reductions in adherence to laminin in all three groups of strains. Penicillin was more successful in reducing the adherence abilities of the tested GAS strains than erythromycin.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Bacterial Adhesion/drug effects ; Erythromycin/pharmacology ; Humans ; Laminin/drug effects ; Microbial Sensitivity Tests ; Penicillins/pharmacology ; Streptococcus pyogenes/drug effects
Chemical Substances	Anti-Bacterial Agents ; Laminin ; Penicillins ; Erythromycin (63937KV33D)
Language	English
Publishing date	2015-08
Publishing country	Brazil
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-02760150092
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Article ; Online: Trends in molecular characteristics and antimicrobial resistance of group B streptococci: a multicenter study in Serbia, 2015-2020.

Kekic, Dusan / Gajic, Ina / Opavski, Natasa / Kojic, Milan / Vukotic, Goran / Smitran, Aleksandra / Boskovic, Lidija / Stojkovic, Marina / Ranin, Lazar

Scientific reports

2021 Volume 11, Issue 1, Page(s) 540

Abstract: Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal ... ...

Abstract	Group B Streptococcus (GBS) is a major cause of neonatal morbidity and mortality. Serbia has not fully implemented preventive measures against GBS neonatal diseases. Therefore, we aimed to assess the maternal GBS colonisation and invasive neonatal disease rate, to reveal the trends of antimicrobial resistance and serotype distribution of GBS from various patient groups. Randomly selected non-invasive (n = 991) and all invasive GBS (n = 80) collected throughout Serbia from 2015 to 2020 were tested for antimicrobial susceptibility, capsular typing, and hvgA detection. Overall, 877/5621 (15.6%) pregnant women were colonised with GBS. Invasive GBS infections incidence in infants (0.18/1000 live births) showed a decreasing trend (0.3 to 0.1/1000 live births). Type III was overrepresented in infants with invasive infections (n = 35, 58.3%), whereas type V predominated among colonised adults (n = 224, 25.5%) and those with noninvasive (n = 37, 32.5%) and invasive infections (n = 8, 40%). The hypervirulent clone III/ST17 was highly associated with invasive infections (n = 28, 35%), particularly late-onset disease (n = 9, 47.4%), showing an increase from 12.3 to 14.8%. The GBS resistance to erythromycin and clindamycin was 26.7% and 22.1%, respectively, with an upward trend. The emergence of the hypervirulent clone III/ST17 and the escalation in GBS resistance highlight an urgent need for continuous monitoring of GBS infections.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Clindamycin/pharmacology ; Drug Resistance, Bacterial/genetics ; Erythromycin/pharmacology ; Female ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases/epidemiology ; Infant, Newborn, Diseases/microbiology ; Male ; Pregnancy ; Pregnancy Complications, Infectious/epidemiology ; Pregnancy Complications, Infectious/microbiology ; Prevalence ; Serbia/epidemiology ; Streptococcal Infections/epidemiology ; Streptococcal Infections/microbiology ; Streptococcus agalactiae/drug effects ; Streptococcus agalactiae/genetics ; Streptococcus agalactiae/pathogenicity ; Time Factors
Chemical Substances	Anti-Bacterial Agents ; Clindamycin (3U02EL437C) ; Erythromycin (63937KV33D)
Language	English
Publishing date	2021-01-12
Publishing country	England
Document type	Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-79354-3
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Article: Influence of subinhibitory antibiotic concentration on Streptococcus pyogenes adherence and biofilm production.

Šmitran, Aleksandra / Vuković, Dragana / Opavski, Nataša / Gajić, Ina / Marinković, Jelena / Božić, Ljiljana / Živanović, Irena / Kekić, Dušan / Popović, Sunčica / Ranin, Lazar

Acta microbiologica et immunologica Hungarica

2018 Volume 65, Issue 2, Page(s) 229–240

Abstract: In this study, the focus was on the effects of sub-MICs of the antibiotics on adherence, hydrophobicity, and biofilm formation by two groups of Streptococcus pyogenes strains, which were responsible for different clinical cases. The aim of this study was ...

Abstract	In this study, the focus was on the effects of sub-MICs of the antibiotics on adherence, hydrophobicity, and biofilm formation by two groups of Streptococcus pyogenes strains, which were responsible for different clinical cases. The aim of this study was to explore the effects of sub-MICs of penicillin, ceftriaxone, erythromycin, and clindamycin on adherence, surface hydrophobicity, and biofilm biomass in two selected collections of group A streptococcus (GAS): strains isolated from carriers (CA) and strains isolated from patients with tonsillopharyngitis (TPh). Isolates were tested for hydrophobicity to xylene, adherence, and biofilm production in uncoated microtiter plates before and after treatment with 1/2 and 1/4 MICs of antibiotics. Penicillin reduced adherence and biofilm production in TPh strains, whereas ceftriaxone diminished adherence and biofilm formation in CA group. On the contrary, clindamycin enhanced adherence and biofilm production in both groups of strains. Erythromycin did not significantly alter adherence, but triggered biofilm production in both groups of isolates. Hydrophobicity of both groups of strains was significantly reduced after exposure to all antibiotics. Beta-lactams displayed anti-biofilm activity; penicillin diminished both adherence and biofilm production in TPh strains, whereas ceftriaxone reduced it in strains isolated from CA.
MeSH term(s)	Anti-Bacterial Agents/administration & dosage ; Anti-Bacterial Agents/pharmacology ; Bacterial Adhesion/drug effects ; Biofilms/growth & development ; Microbial Sensitivity Tests ; Streptococcus pyogenes/drug effects ; Streptococcus pyogenes/physiology
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2018-04-24
Publishing country	Hungary
Document type	Journal Article
ZDB-ID	918256-1
ISSN	1588-2640 ; 1217-8950
ISSN (online)	1588-2640
ISSN	1217-8950
DOI	10.1556/030.65.2018.026
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Article ; Online: The effect of hydrophobicity of group a beta-hemolytic streptococcus in the process of adherence and biofilm production

Šmitran Aleksandra / Marković Tatjana / Ranin Lazar

Srpski Arhiv za Celokupno Lekarstvo, Vol 142, Iss 9-10, Pp 557-

2014 Volume 563

Abstract: Introduction. Bacterial cell hydrophobicity and adherence to a substrate are one of the most important factors in biofilm formation. Group A streptococcus is an unstable and low biofilm productor. Importance of biofilm production in streptococcal ... ...

Abstract	Introduction. Bacterial cell hydrophobicity and adherence to a substrate are one of the most important factors in biofilm formation. Group A streptococcus is an unstable and low biofilm productor. Importance of biofilm production in streptococcal pathogenesis is still unknown. Objective. The aim of this study was to determine the impact of hydrophobicity and adherence on the biofilm production of group A streptococcal invasive and noninvasive isolates, and also to evaluate the stability of biofilm production in time function. Methods. Adherence, hydrophobicity and biofilm production were investigated in a total of 172 isolates divided into three groups: noninvasive, low invasive and highly invasive. Adherence to uncoated and laminin-coated microtiter plates and biofilm production after 12, 24 and 48 hours of incubation was determined using the method described by Stepanović et al. Hydrophobicity was measured using the MATH test by Rosenberg and SAT test by Lindhal. Results. Correlation between adherence and biofilm production was detected in the group of noninvasive isolates. These isolates were stable biofilm productors during all three time periods of biofilm production. In the groups of invasive and noninvasive isolates no statistical correlation was detected among the analysed variables. The invasive isolates were unstable biofilm productors. Conclusion. Noninvasive isolates were stable biofilm producers; as detected, they showed a direct correlation between adherence and biofilm production, and a negative impact of hydrophobicity on the biofilm production. Invasive isolates were unstable biofilm productors; it was observed that there was no correlation between adherence and hydrophobicity with biofilm production. [Projekat Ministarstva nauke Republike Srbije, br. 175039: Bakterije rezistentne na antibiotike u Srbiji: fenotipska i genotipska rezistencija]
Keywords	adherence ; hydrophobicity ; biofilm ; group A streptococcus ; Medicine ; R
Subject code	630
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	Serbian Medical Society
Document type	Article ; Online
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Article ; Online: Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.

Radovanovic, Radoslava Savic / Savic, Natasa Rajic / Ranin, Lazar / Smitran, Aleksandra / Opavski, Natasa Vuckovic / Tepavcevic, Andreja Milosavljevic / Ranin, Jovana / Gajic, Ina

Current microbiology

2020 Volume 77, Issue 12, Page(s) 4045–4052

Abstract: Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas ... ...

Abstract	Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108 isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98 (90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The bla
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Biofilms ; Drug Resistance, Bacterial ; Food Microbiology ; Humans ; Microbial Sensitivity Tests ; Pseudomonas/drug effects ; Pseudomonas/genetics ; Pseudomonas Infections ; Pseudomonas aeruginosa/drug effects ; Pseudomonas aeruginosa/genetics ; beta-Lactamases
Chemical Substances	Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
Language	English
Publishing date	2020-10-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	134238-1
ISSN	1432-0991 ; 0343-8651
ISSN (online)	1432-0991
ISSN	0343-8651
DOI	10.1007/s00284-020-02236-4
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Article ; Online: Tigecycline susceptibility of multidrug-resistant Acinetobacter baumannii from intensive care units in the western Balkans.

Gajic, Ina / Ranin, Lazar / Kekic, Dusan / Opavski, Natasa / Smitran, Aleksandra / Mijac, Vera / Jovanovic, Snezana / Hadnadjev, Mirjana / Travar, Maja / Mijovic, Gordana

Acta microbiologica et immunologica Hungarica

2020 Volume 67, Issue 3, Page(s) 176–181

Abstract: Tigecycline can be effective to treat infections of carbapenem-resistant Acinetobacter baumannii (CRAB) however, no interpretive criteria have been approved so far. The objectives of this study were to evaluate the proportion of CRAB isolates and to ... ...

Abstract	Tigecycline can be effective to treat infections of carbapenem-resistant Acinetobacter baumannii (CRAB) however, no interpretive criteria have been approved so far. The objectives of this study were to evaluate the proportion of CRAB isolates and to compare gradient test with a broth microdilution (BMD) method for tigecycline susceptibility testing of A. baumannii.This study included 349 multidrug-resistant (MDR) Acinetobacter spp. collected from Serbia, Montenegro, Bosnia and Herzegovina in 2016 and 2017. Antibiotic susceptibility testing was performed by disk diffusion, VITEK2, gradient, ComASP Colistin. Tigecycline susceptibilities were interpreted according to breakpoints of European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Food and Drug Administration (FDA).Majority of the tested isolates were CRAB (92.8%). Tigecycline MIC50/MIC90 values were 4/8 μg/mL by BMD and 0.5/4 μg/mL by gradient test. Essential agreement for BMD and gradient test amounted to 65.1%. With EUCAST breakpoints, categorical agreement (CA) was achieved in 38% isolates. Major discordance (MD-false susceptibility/resistance) and minor discordance (mD-false categorization involving intermediate results) were observed in 10% and 57% A. baumannii, respectively. With FDA breakpoints, CA, MD and mD were observed in 44%, 16% and 47% isolates, respectively. Colistin resistance was 2.1%.The study highlights a high proportion of CRAB and several discordances between BMD and gradient test which may lead to inappropriate therapy.
MeSH term(s)	Acinetobacter Infections/epidemiology ; Acinetobacter Infections/microbiology ; Acinetobacter baumannii/drug effects ; Acinetobacter baumannii/isolation & purification ; Anti-Bacterial Agents/pharmacology ; Balkan Peninsula/epidemiology ; Carbapenems/pharmacology ; Drug Resistance, Multiple, Bacterial ; Hospitalization ; Humans ; Intensive Care Units ; Microbial Sensitivity Tests ; Tigecycline/pharmacology
Chemical Substances	Anti-Bacterial Agents ; Carbapenems ; Tigecycline (70JE2N95KR)
Language	English
Publishing date	2020-03-09
Publishing country	Hungary
Document type	Journal Article ; Multicenter Study
ZDB-ID	918256-1
ISSN	1588-2640 ; 1217-8950
ISSN (online)	1588-2640
ISSN	1217-8950
DOI	10.1556/030.2020.01079
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