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  1. Book ; Online: KE-QI

    Ai, Chunhui / Wang, Derui / Yan, Xu / Xu, Yang / Xie, Wenrui / Cao, Ziqiang

    A Knowledge Enhanced Article Quality Identification Dataset

    2022  

    Abstract: ... entities to Baidu Encyclopedia, then propose Knowledge Enhanced article Quality Identification (KE-QI ... results on KE-QI show that with initialization of our pre-trained Node2Vec model, our model achieves ...

    Abstract With so many articles of varying qualities being produced every moment, it is a very urgent task to screen outstanding articles and commit them to social media. To our best knowledge, there is a lack of datasets and mature research works in identifying high-quality articles. Consequently, we conduct some surveys and finalize 7 objective indicators to annotate the quality of 10k articles. During annotation, we find that many characteristics of high-quality articles (e.g., background) rely more on extensive external knowledge than inner semantic information of articles. In response, we link extracted article entities to Baidu Encyclopedia, then propose Knowledge Enhanced article Quality Identification (KE-QI) dataset. To make better use of external knowledge, we propose a compound model which fuses the text and external knowledge information via a gate unit to classify the quality of an article. Our experimental results on KE-QI show that with initialization of our pre-trained Node2Vec model, our model achieves about 78\% $F_1$, outperforming other baselines.
    Keywords Computer Science - Computation and Language
    Subject code 306
    Publishing date 2022-06-15
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article: [Two new sesquiterpenes in Qi-nan Aquilariae Lignum Resinatum].

    Chen, De-Li / Ma, Guo-Xu / Liu, Hui-Mei / Wang, Can-Hong / Liu, Yang-Yang / Yang, Yun / Wei, Jian-He

    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

    2024  Volume 48, Issue 23, Page(s) 6403–6407

    Abstract: This study aimed to investigate the chemical constituents of supercritical extract from Qi-nan ...

    Abstract This study aimed to investigate the chemical constituents of supercritical extract from Qi-nan Aquilariae Lignum Resinatum by silica gel column chromatography, thin-layer chromatography, and semi-preparative high-performance liquid chromatography. One new elemane-type and one new eudesmane-type sesquiterpene compounds were isolated from the extract, and their structures were identified by MS, UV, IR, NMR, and ECD spectroscopic techniques, and named aquqinanol C(1) and aquqinanol D(2). Both compounds are novel compounds. The neuroprotective effect of the compounds on CORT-induced PC12 cell damage was determined in vitro. The results showed that compounds 1 and 2 exhibited a certain protective effect against CORT-induced damage in PC12 cells.
    MeSH term(s) Rats ; Animals ; Qi ; Sesquiterpenes/pharmacology ; Molecular Structure
    Chemical Substances Sesquiterpenes
    Language Chinese
    Publishing date 2024-01-09
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 1004649-5
    ISSN 1001-5302 ; 0254-0029
    ISSN 1001-5302 ; 0254-0029
    DOI 10.19540/j.cnki.cjcmm.20230618.203
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The San-Qi-Xue-Shang-Ning formula protects against ulcerative colitis by restoring the homeostasis of gut immunity and microbiota.

    Yu, Wei / Kang, Cai / Zhang, Yijia / Li, Qi / Zhang, Zhiqiang / Zheng, Yang / Liu, Xincheng / Yan, Jing

    Journal of ethnopharmacology

    2023  Volume 305, Page(s) 116125

    Abstract: ... associated cancer (CAC). The San-Qi-Xue-Shang-Ning (SQ) formula has been utilized in clinical practice ...

    Abstract Ethnopharmacological relevance: Ulcerative colitis (UC) is a major cause of morbidity and mortality due to repetitive remissions and relapses, and many severe complications, including colitis-associated cancer (CAC). The San-Qi-Xue-Shang-Ning (SQ) formula has been utilized in clinical practice to treat gut diseases, but its pharmacological evidence is limited and awaits elucidation.
    Aim of the study: Here, we elucidated the molecular mechanisms of the SQ formula.
    Materials and methods: Its therapeutic value in combating UC and CAC was predicted from network pharmacology and weighted gene co-expression network analysis (WGCNA). Experimental colitis models were established by feeding dextran sodium sulfate (DSS) to C57BL/6N mice for 7 days, and they were subjected to the SQ formula for 14 days. High-throughput technologies and biochemical investigations were executed to corroborate the anti-colitis effect.
    Results: Network pharmacology and WGCNA demonstrated that the targets of the SQ formula were associated with interleukin-17 (IL-17), tumor necrosis factor (TNF), IL-1b and peroxisome proliferators-activated receptor (PPAR) signaling pathways, and correlated with the survival in patients with colorectal cancer. In mice with colitis, the SQ treatment hindered colitis progression in a dose-dependent manner, as evidenced by the rescued colon length and weight loss, improved colonic epithelial integrity, and abolished crypt loss. In addition to the suppressed serum IL-17, TNFα, and IL-1b levels, the SQ-treated colitis mice exhibited decreased colonic protein abundance of hypoxia-inducible factor-1α (HIF-1 α), PPARα, and Caspase3 (Casp3) with an increased PPARγ expression. Concurrently, the high dose of SQ promoted the alternative activation of peritoneal macrophages by increasing Arg1 and inhibiting iNOS2, thereby facilitating the migration of NCM460 cells and controlling TNF-induced reactive oxygen species production and apoptosis in intestinal organoids. In colitis-accompanied dysbiosis, the SQ formula reversed the decreased microbiota diversity indexes and restored the microbiome profile in the murine colitis models.
    Conclusion: The SQ formula is a potent anti-colitis drug that facilitates inflammation resolution and restores gut microbiota homeostasis.
    MeSH term(s) Mice ; Animals ; Colitis, Ulcerative/chemically induced ; Colitis, Ulcerative/drug therapy ; Colitis, Ulcerative/metabolism ; Interleukin-17/metabolism ; Mice, Inbred C57BL ; Colitis/chemically induced ; Colon ; Microbiota ; Homeostasis ; Dextran Sulfate/toxicity ; Disease Models, Animal
    Chemical Substances Interleukin-17 ; Dextran Sulfate (9042-14-2)
    Language English
    Publishing date 2023-01-02
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2022.116125
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Luteolin in the Qi Bi Anshen decoction improves propionic acid-induced autism-like behavior in rats by inhibiting LRP1/MMP9.

    Zhang, Mengjia / Yu, Jiaoyan / Liu, An / Liu, Qing-Qing / Sun, Ting / Li, Xi / Du, Yaya / Li, Jiamin / Wang, Bin / Yang, Qi

    Phytomedicine : international journal of phytotherapy and phytopharmacology

    2023  Volume 118, Page(s) 154965

    Abstract: ... can eliminate the symptoms of autism by precisely regulating human physiology. The Qi Bi Anshen decoction (QAT ...

    Abstract Background: A neurodevelopmental illness with a high frequency and unidentified pathophysiology is known as autism spectrum disorder (ASD). A research hotspot in this field is the identification of disease-specific biomarkers and drug intervention targets. Traditional Chinese medicine (TCM) can eliminate the symptoms of autism by precisely regulating human physiology. The Qi Bi Anshen decoction (QAT) is a commonly used TCM clinical drug commonly-used to treat for treating ASD. However, the primary active ingredients and underlying mechanisms of action of this decoction remain unknown.
    Purpose: This study aimed to investigate the active ingredients and pharmacodynamics of QAT in the treatment of ASD using a Sprague-Dawley rat model that resembled autism.
    Methods: Autism-like rat models were established through intracerebroventricular injections of propionic acid (PPA). Subsequently, the rats were treated with QAT, and their efficacy was evaluated using the three-chamber method to analyze social interactions and grooming behavior. Additionally, open-field tests, elevated cross-maze tests, hematoxylin and eosin staining, Nissl staining, and enzyme-linked immunosorbent assays were performed; Western blot analysis was employed to determine the expression of synaptic plasticity-related proteins. Utilizing ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS), the effectiveness of active QAT components was assessed, and potential QAT targets were screened through molecular docking, surface plasmon resonance, and thermal migration experiments. To better understand the precise processes involved in treating ASD with active QAT components, in vivo and in vitro knockdown tests were also performed.
    Results: QATexhibited a significant improvement in autism-like behavior and a notable increase in the production of proteins associated with synaptic plasticity. Furthermore, luteolin (LUT), identified as a potentially important active ingredient in QAT for treating ASD, reduced matrix metallopeptidase-9 (MMP9) expression. However, this effect was attenuated by the knockdown of low-density lipoprotein receptor-associated protein 1 (LRP1), which is the target binding site for LUT.
    Conclusions: LUT emerges as a potentially crucial active component of QAT in the treatment of ASD, with the ability to antagonize LRP1 and subsequently reduce MMP9 expression.
    MeSH term(s) Rats ; Animals ; Humans ; Autistic Disorder/chemically induced ; Autistic Disorder/drug therapy ; Autism Spectrum Disorder/chemically induced ; Autism Spectrum Disorder/drug therapy ; Autism Spectrum Disorder/diagnosis ; Luteolin/therapeutic use ; Matrix Metalloproteinase 9 ; Receptors, Lipoprotein ; Chromatography, Liquid ; Molecular Docking Simulation ; Qi ; Rats, Sprague-Dawley ; Tandem Mass Spectrometry ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Low Density Lipoprotein Receptor-Related Protein-1/therapeutic use
    Chemical Substances propionic acid (JHU490RVYR) ; Luteolin (KUX1ZNC9J2) ; Matrix Metalloproteinase 9 (EC 3.4.24.35) ; Receptors, Lipoprotein ; Drugs, Chinese Herbal ; LRP1 protein, human ; Low Density Lipoprotein Receptor-Related Protein-1 ; MMP9 protein, human (EC 3.4.24.35)
    Language English
    Publishing date 2023-07-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.154965
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Clinical Value of Heart Qi in the Treatment of Chronic Heart Failure With Depression.

    Wang, Ying / Lv, Wang / Chen, Hu / Yang, Qian / Zhang, Yang

    Alternative therapies in health and medicine

    2023  Volume 30, Issue 2, Page(s) 36–41

    Abstract: ... studied the effects of tonic heart qi soup for patients with CHF and depression.: Objective: The study ... intended to evaluate the clinical efficacy of tonic heart qi soup in the treatment of chronic heart failure ... which received conventional Western medical treatment combined with treatment with a commercial tonic heart qi ...

    Abstract Context: Chronic heart failure (CHF) is a form of persistent heart failure. If a patient develops depression, it can worsen the severity of heart failure and can lead to adverse outcomes. No researchers have studied the effects of tonic heart qi soup for patients with CHF and depression.
    Objective: The study intended to evaluate the clinical efficacy of tonic heart qi soup in the treatment of chronic heart failure (CHF) for patients with comorbid depression.
    Design: The research team performed a prospective randomized controlled trial.
    Setting: The study took place in the Department of Chinese Medicine at Cangzhou Central Hospital in Cangzhou, Hebei Province, China.
    Participants: Participants were 120 patients with CHF at the hospital as inpatients or outpatients between January 2016 and January 2019.
    Intervention: The research team divided participants into two groups, with 60 patients each: (1) an intervention group, which received conventional Western medical treatment combined with treatment with a commercial tonic heart qi soup and (2) a control group, which received conventional Western medical treatment only.
    Outcome measures: The research team measured: (1) treatment efficacy, (2) cardiac function, (3) adverse reactions, (4) B-type natriuretic peptide (BNP) and Ghrelin, and (5) depression.
    Results: In the intervention group, 55 participants showed significant improvement in the degree of heart failure, for a total effectiveness rate of 91.67%, which was significantly higher than that of the control group (P = .000). The intervention group had 10 participants in class II, 18 in class III, and 22 in class IV. Among them, 28 participants improved, indicating significantly better outcomes than those of the control group. The intervention group's BNP levels, at 1031.58 ± 118.83 pg/ml, and ghrelin levels, at 481.46 ± 57.53%, were significantly lower than those of the control group. No liver- or renal-function damage, insomnia, or significant adverse reactions occurred for either group. The intervention group's total incidence rate for adverse reactions, at 1.67%, was significantly lower than that of the control group, at 11.67% (P = .000) and also had a higher total effective rate in reducing depression, at 86.67%, compared to that of the control group, at 43.33%.
    Conclusions: Heart Qi Tonic Tang, as an adjunctive therapy, significantly improved outcomes for CHF patients with depression. It effectively reduced heart failure symptoms, with minimal adverse reactions and increased patient comfort and compliance.
    MeSH term(s) Humans ; Ghrelin/therapeutic use ; Qi ; Depression/therapy ; Prospective Studies ; Heart Failure/therapy ; Heart Failure/drug therapy
    Chemical Substances Ghrelin
    Language English
    Publishing date 2023-10-19
    Publishing country United States
    Document type Randomized Controlled Trial ; Journal Article
    ZDB-ID 1225073-9
    ISSN 1078-6791
    ISSN 1078-6791
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: UPLC-QTOF-MS based metabolomics unravels the modulatory effect of ginseng water extracts on rats with Qi-deficiency.

    Li, Yanyi / Wu, Yi / Li, Hanlin / Wang, Meiyuan / Gao, Yang / Pei, Shuhua / Liu, Shu / Liu, Zhiqiang / Liu, Zhongying / Men, Lihui

    Journal of pharmaceutical and biomedical analysis

    2024  Volume 242, Page(s) 116019

    Abstract: ... to invigorate qi. As a result, individuals with Qi-deficiency often use ginseng as a health supplement ... mechanisms of action of these components in Qi-deficiency remain unclear. This study aimed to determine ... in a rat model of Qi-deficiency using metabolomics and network analysis. The rat model of Qi-deficiency was ...

    Abstract Ginseng is commonly used as a nutritional supplement and daily wellness product due to its ability to invigorate qi. As a result, individuals with Qi-deficiency often use ginseng as a health supplement. Ginsenosides and polysaccharides are the primary components of ginseng. However, the therapeutic effects and mechanisms of action of these components in Qi-deficiency remain unclear. This study aimed to determine the modulatory effects and mechanisms of ginseng water extract, ginsenosides, and ginseng polysaccharides in a rat model of Qi-deficiency using metabolomics and network analysis. The rat model of Qi-deficiency was established via swimming fatigue and a restricted diet. Oral administration of different ginseng water extracts for 30 days primarily alleviated oxidative stress and disrupted energy metabolism and immune response dysfunction caused by Qi-deficiency in rats. Ultra-high-performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for untargeted serum metabolomic analysis. Based on the analysis results, the active constituents of ginseng significantly reversed the changes in serum biomarkers related to Qi-deficiency in rats, particularly energy, amino acid, and unsaturated fatty acid metabolism. Furthermore, analysis of the metabolite-gene network suggested that the anti-Qi-deficiency effects of the ginseng components were mainly associated with toll-like receptor (TLR) signaling and inflammatory response. Additional verification revealed that treatment with the ginseng components effectively reduced the inflammatory response and activation of the myocardial TLR4/NF-κB pathway induced by Qi-deficiency, especially the ginseng water extracts. Therefore, ginseng could be an effective preventive measure against the progression of Qi-deficiency by regulating metabolic and inflammatory responses.
    MeSH term(s) Rats ; Animals ; Chromatography, High Pressure Liquid/methods ; Ginsenosides/analysis ; Metabolomics/methods ; Panax/chemistry ; Polysaccharides
    Chemical Substances Ginsenosides ; Polysaccharides
    Language English
    Publishing date 2024-02-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 604917-5
    ISSN 1873-264X ; 0731-7085
    ISSN (online) 1873-264X
    ISSN 0731-7085
    DOI 10.1016/j.jpba.2024.116019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Chinese herbal decoction, Yi-Qi-Jian-Pi formula exerts anti-hepatic fibrosis effects in mouse models of CCl

    Yang, Shiyan / Cheng, Yajun / Wang, Xiaolong / Yue, Suyang / Wang, Xi / Tang, Li / Li, Hailun / Zhang, Jie / Xiong, Qingping / Tan, Shanzhong

    Heliyon

    2024  Volume 10, Issue 5, Page(s) e26129

    Abstract: Background: Yi-Qi-Jian-Pi Formula (YQJPF) is a herbal medicine that is used to treat patients ...

    Abstract Background: Yi-Qi-Jian-Pi Formula (YQJPF) is a herbal medicine that is used to treat patients with liver failure. However, scientific evidence supporting the treatment of hepatic fibrosis with YQJPF has not been forthcoming. The present study aimed to determine the mechanisms underlying the anti-fibrotic effects of YQJPF in mouse models of hepatic fibrosis.
    Methods: Mice were randomly assigned to control, hepatic fibrosis model, silymarin (positive treated), and low-, medium- and high-dose YQJPF (7.5, 15, and 30 g/kg, respectively) groups. Liver function, inflammatory cytokines, and oxygen stress were analyzed using ELISA kits. Sections were histopathologically stained with hematoxylin-eosin, Masson trichrome, and Sirius red. Macrophage polarization was measured by flow cytometry and immunofluorescence. Potential targets of YQJPF against hepatic fibrosis were analyzed by network pharmacology of Chinese herbal compound and the effects of YQJPF on the transforming growth factor-beta (TGF-β)/Suppressor of Mothers against Decapentaplegic family member 3 (Smad3) signaling pathway were assessed using qRT-PCR and immunohistochemical staining. Finally, metagenomics and LC-MS/MS were used to detect the intestinal flora and metabolites of the mice, and an in-depth correlation analysis was performed by spearman correlation analysis. The data were compared by one-way ANOVA and least significant differences (LSDs) or ANOVA-Dunnett's T3 method used when no homogeneity was detected.
    Results: We induced hepatic fibrosis using CCl
    Conclusions: YQJPF can reverse liver fibrosis by inhibiting inflammation, suppressing oxidative stress, regulating the immunological response initiated by macrophages, inhibiting TGF-β/Smad3 signaling and regulating intestinal flora homeostasis. Therefore, YQJPF may be included in clinical regimens to treat hepatic fibrosis.
    Language English
    Publishing date 2024-02-22
    Publishing country England
    Document type Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e26129
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The effect of compound Da-Cheng-Qi Decoction on the treatment of malignant bowel obstruction with transnasal ileus tube.

    Qi, Xiang / Shimin, Yang / Yu, Wu

    Complementary therapies in clinical practice

    2021  Volume 43, Page(s) 101316

    Abstract: Objective: To study the effect of compound Da-Cheng-Qi Decoction (CDCQD) on the treatment ...

    Abstract Objective: To study the effect of compound Da-Cheng-Qi Decoction (CDCQD) on the treatment of malignant bowel obstruction (MBO) with transnasal ileus tube (TIT).
    Methods: We observed 30 cases of MBO from July 2018 to August 2019. The patients were divided into the control group (n = 15) and the CDCQD group (n = 15) according to a random number table. All patients were inserted the TIT after admission. Twenty-four hours later, the CDCQD group began to take 100 ml CDCQD twice a day for 7 days. The control group took the plain boiled water instead. Other treatment was the same in the two groups. The waistline reduction, the release time of abdominal pain and distention, recovery of exhaust and defecation time, drainage volume of TIT were observed and compared between the two groups.
    Results: Three days after insertion of TIT, the abdominal plain film was re-examined in the two groups. Most of the patients' gas-liquid level disappeared and there was no significant difference between the two groups (P > 0.05). The effective rate of CDCQD group (86.7%) was significantly higher than that of control group (53.3%). The recovery time of exhaust and defecation in the CDCQD group was earlier than that in the control group (P < 0.05). The daily drainage volume of TIT in the CDCQD group was less than that in the control group, especially from the fourth day to the sixth day after insertion of TIT, with a significant difference (P < 0.05).
    Conclusion: TIT is an effective treatment for patients with MBO. With the basis of TIT treatment, CDCQD therapy can improve the curative effect of MBO. It can promote intestinal exhaust and defecation and improves the curative effect of palliative treatment of MBO. It is an effective method to assist Tit in the treatment for MBO patients.
    MeSH term(s) Drugs, Chinese Herbal/therapeutic use ; Humans ; Ileus/drug therapy ; Ileus/etiology ; Intestinal Obstruction/drug therapy ; Palliative Care
    Chemical Substances Da-Cheng-Qi ; Drugs, Chinese Herbal
    Language English
    Publishing date 2021-01-27
    Publishing country England
    Document type Journal Article
    ZDB-ID 2182834-9
    ISSN 1873-6947 ; 1744-3881
    ISSN (online) 1873-6947
    ISSN 1744-3881
    DOI 10.1016/j.ctcp.2021.101316
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Luteolin in the Qi Bi Anshen decoction improves propionic acid-induced autism-like behavior in rats by inhibiting LRP1/MMP9

    Zhang, Mengjia / Yu, Jiaoyan / Liu, An / Liu, Qing-Qing / Sun, Ting / Li, Xi / Du, Yaya / Li, Jiamin / Wang, Bin / Yang, Qi

    Phytomedicine. 2023 Sept., v. 118 p.154965-

    2023  

    Abstract: ... by precisely regulating human physiology. The Qi Bi Anshen decoction (QAT) is a commonly used TCM clinical drug ...

    Abstract A neurodevelopmental illness with a high frequency and unidentified pathophysiology is known as autism spectrum disorder (ASD). A research hotspot in this field is the identification of disease-specific biomarkers and drug intervention targets. Traditional Chinese medicine (TCM) can eliminate the symptoms of autism by precisely regulating human physiology. The Qi Bi Anshen decoction (QAT) is a commonly used TCM clinical drug commonly-used to treat for treating ASD. However, the primary active ingredients and underlying mechanisms of action of this decoction remain unknown. This study aimed to investigate the active ingredients and pharmacodynamics of QAT in the treatment of ASD using a Sprague–Dawley rat model that resembled autism. Autism-like rat models were established through intracerebroventricular injections of propionic acid (PPA). Subsequently, the rats were treated with QAT, and their efficacy was evaluated using the three-chamber method to analyze social interactions and grooming behavior. Additionally, open-field tests, elevated cross-maze tests, hematoxylin and eosin staining, Nissl staining, and enzyme-linked immunosorbent assays were performed; Western blot analysis was employed to determine the expression of synaptic plasticity-related proteins. Utilizing ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS), the effectiveness of active QAT components was assessed, and potential QAT targets were screened through molecular docking, surface plasmon resonance, and thermal migration experiments. To better understand the precise processes involved in treating ASD with active QAT components, in vivo and in vitro knockdown tests were also performed. QATexhibited a significant improvement in autism-like behavior and a notable increase in the production of proteins associated with synaptic plasticity. Furthermore, luteolin (LUT), identified as a potentially important active ingredient in QAT for treating ASD, reduced matrix metallopeptidase-9 (MMP9) expression. However, this effect was attenuated by the knockdown of low-density lipoprotein receptor-associated protein 1 (LRP1), which is the target binding site for LUT. LUT emerges as a potentially crucial active component of QAT in the treatment of ASD, with the ability to antagonize LRP1 and subsequently reduce MMP9 expression.
    Keywords Oriental traditional medicine ; Western blotting ; active ingredients ; animal models ; autism ; biomarkers ; drugs ; eosin ; human physiology ; liquid chromatography ; low density lipoprotein ; luteolin ; mass spectrometry ; neuroplasticity ; pathophysiology ; pharmacodynamics ; propionic acid ; rats ; surface plasmon resonance ; Autism spectrum disorder ; Qi Bi Anshen decoction ; LRP1 ; MMP9
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier GmbH
    Document type Article ; Online
    ZDB-ID 1205240-1
    ISSN 1618-095X ; 0944-7113
    ISSN (online) 1618-095X
    ISSN 0944-7113
    DOI 10.1016/j.phymed.2023.154965
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: Yi Qi Chu Tan Formula (YQCTF) inhibited the progress of lung cancer via regulating tumor-associated neutrophil: An integrated study of network pharmacology, proteomics and pharmacodynamics.

    Le-Xin, Chen / Ming-Jun, Li / Chun-Qi, Xu / Jia-Xin, Zeng / Jing-Ya, Yang / Li-Xin, Nie / Mei-Qi, Wang / En-Xin, Zhang / Xiao-Jun, Zhang

    Journal of ethnopharmacology

    2023  Volume 318, Issue Pt B, Page(s) 116943

    Abstract: Ethnopharmacological relevance: Yi Qi Chu Tan Formula (YQCTF), a prescription consisting of eight ...

    Abstract Ethnopharmacological relevance: Yi Qi Chu Tan Formula (YQCTF), a prescription consisting of eight traditional Chinese medicine for treating lung cancer, has been clinically proven to be effective in improving the life quality and prolonging the survival time of non-small cell lung cancer (NSCLC) patients.
    Aim of the study: This study aimed to evaluate the therapeutic efficacy of YQCTF on NSCLC mice model and further explore its therapeutic targets by using network pharmacology, proteomics and pharmacodynamic methodologies.
    Materials and methods: The network pharmacology analysis was firstly conducted to screen out the potential active ingredients and therapeutic targets of YQCTF against NSCLC. Three kinds of extracts, i.e. the water extract (WE), water extraction-alcohol precipitation (WEAP) and alcohol extract (AE) of YQCTF were prepared, which chemical compositions were subsequently analyzed by using ultra-high performance liquid chromatography-mass spectrometry/mass spectrometry (UPLC-MS/MS), and which anti-neoplastic efficacy was examined on NSCLC mice model. Mice tumor tissues were collected for proteomics analysis, and the immunomodulatory effects of YQCTF extracts on the tumor microenvironment (TME) were further validated by using flow cytometry, immunofluorescence, ELISA and Western blot.
    Results: Network pharmacology identified 60 conjunct genes and ample cancer-related signaling pathways as potential therapeutic targets of YQCTF. Protein-protein interaction (PPI), Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that YQCTF might negatively regulate cancer-related inflammation. UPLC-MS/MS analysis showed that the main components of YQCTF include at least ginsenosides, solasodine, solamargine, solasonine, peimisine, peiminine, peimine and sipeimine-3β-D-glucosihde. All kinds of YQCTF extracts significantly inhibited the growth of lung cancer allograft and regulated the ratio of immune cells in tumor tissues, i.e. upregulated the fractions of T cells, promoted the maturation of dendritic cells (DCs), increased the M1/M2 ratio of tumor-related macrophages, but reduced the number of Tregs and immunosuppressive neutrophils. Proteomics identified neutrophils to be the most prominently enriched target linked to NETs formation in mice tumor tissue, which is verified by the downregulation of neutrophil recruiting factors involving IL-6, HIF-1α and IL-8, as well as the decreases of NETs-related biomarkers including H3cit, MPO, CD18, MMP9 and ICAM-1 in immunofluorescence, ELISA and Western blot analysis.
    Conclusion: YQCTF inhibited the progress of mice NSCLC allograft, suppressed the pro-tumorigenic tumor-associated neutrophils and improved the tumor immune microenvironment (TIME).
    MeSH term(s) Animals ; Mice ; Lung Neoplasms/drug therapy ; Carcinoma, Non-Small-Cell Lung/drug therapy ; Neutrophils ; Chromatography, Liquid ; Network Pharmacology ; Proteomics ; Tandem Mass Spectrometry ; Drugs, Chinese Herbal/pharmacology ; Drugs, Chinese Herbal/therapeutic use ; Molecular Docking Simulation ; Tumor Microenvironment
    Chemical Substances Drugs, Chinese Herbal
    Language English
    Publishing date 2023-08-01
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 134511-4
    ISSN 1872-7573 ; 0378-8741
    ISSN (online) 1872-7573
    ISSN 0378-8741
    DOI 10.1016/j.jep.2023.116943
    Database MEDical Literature Analysis and Retrieval System OnLINE

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