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Article ; Online: Recent advances in degradation of N,N-diethyl-3-toluamide (DEET)-an emerging environmental contaminant: a review.

Rani, Ritu / Kumar, Dharmender

2024 Volume 196, Issue 3, Page(s) 238

Abstract: N,N-Diethyl-3-toluamide (DEET) is a commonly used insect repellent, which acts as an organic chemical contaminant in water and considered as an emerging contaminant which has been observed worldwide. It gets discharged into the environment through sewage ...

Abstract	N,N-Diethyl-3-toluamide (DEET) is a commonly used insect repellent, which acts as an organic chemical contaminant in water and considered as an emerging contaminant which has been observed worldwide. It gets discharged into the environment through sewage waste. The various methods have been used to degrade DEET, such as UV based, ozonation, photocatalytic degradation, and biodegradation (based on the metabolic activity of fungi and bacteria). However, less research has been done on the degradation of DEET by deploying nanoparticles. Therefore, biodegradation and nanotechnology-based methods can be the potential solution to remediate DEET from the environment. This review is an attempt to analyze the routes of entry of DEET into the atmosphere and its environmental health consequences and to explore physical, chemical, and biological methods of degradation. Furthermore, it focuses on the various methods used for the biodegradation of the DEET, including their environmental consequences. Future research is needed with the application of biological methods for the degradation of DEET. Metabolic pathway for biodegradation was explored for the new potent microbial strains by the application of physical, chemical, and microbial genomics; molecular biology; genetic engineering; and genome sequencing methods.
MeSH term(s)	DEET/toxicity ; Environmental Monitoring ; Atmosphere ; Biodegradation, Environmental ; Environmental Health
Chemical Substances	DEET (134-62-3)
Language	English
Publishing date	2024-02-06
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	782621-7
ISSN	1573-2959 ; 0167-6369
ISSN (online)	1573-2959
ISSN	0167-6369
DOI	10.1007/s10661-024-12414-7
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Article: Characterization and biodegradation of paracetamol by biomass of

Chopra, Sunil / Kumar, Dharmender

Rendiconti Lincei. Scienze fisiche e naturali

2023 Volume 34, Issue 2, Page(s) 491–501

Abstract: Industrialization leads to the entry of diverse xenobiotic compounds into the environment. One such compound is paracetamol (APAP), which is emerging as a pharmaceutical and personal care pollutant (PPCP). In this study, the APAP degrading bacterium was ... ...

Abstract	Industrialization leads to the entry of diverse xenobiotic compounds into the environment. One such compound is paracetamol (APAP), which is emerging as a pharmaceutical and personal care pollutant (PPCP). In this study, the APAP degrading bacterium was isolated by enrichment culture method from the sewage sample. The microscopy, biochemical, and 16S rRNA gene sequence analyzed the isolate PPY-2, which belongs to
Language	English
Publishing date	2023-02-23
Publishing country	Italy
Document type	Journal Article
ISSN	2037-4631
ISSN	2037-4631
DOI	10.1007/s12210-023-01140-w
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Article ; Online: Perspectives of nanomaterials in microbial remediation of heavy metals and their environmental consequences: A review.

Malik, Sachin / Kumar, Dharmender

Biotechnology & genetic engineering reviews

2023 , Page(s) 1–48

Abstract: Nanomaterials (NMs) have diverse applications in various sectors, such as decontaminating heavy metals from drinking water, wastewater, and soil. Their degradation efficiency can be enhanced through the application of microbes. As microbial strain ... ...

Abstract	Nanomaterials (NMs) have diverse applications in various sectors, such as decontaminating heavy metals from drinking water, wastewater, and soil. Their degradation efficiency can be enhanced through the application of microbes. As microbial strain releases enzymes, which leads to the degradation of HMs. Therefore, nanotechnology and microbial-assisted remediation-based methods help us develop a remediation process with practical utility, speed, and less environmental toxicity. This review focuses on the success achieved for the bioremediation of heavy metals by nanoparticles and microbial strains and in their integrated approach. Still, the use of NMs and heavy metals (HMs) can negatively affect the health of living organisms. This review describes various aspects of the bioremediation of heavy materials using microbial nanotechnology. Their safe and specific use supported by bio-based technology paves the way for their better remediation. We discuss the utility of nanomaterials for removing heavy metals from wastewater, toxicity studies and issues to the environment with their practical implications. Nanomaterial assisted heavy metal degradation coupled with microbial technology and disposal issues are described along with detection methods. Environmental impact of nanomaterials is also discussed based on the recent work conducted by the researchers. Therefore, this review opens new avenues for future research with an impact on the environment and toxicity issues. Also, applying new biotechnological tools will help us develop better heavy metal degradation routes.
Language	English
Publishing date	2023-03-05
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	49457-4
ISSN	2046-5556 ; 0264-8725
ISSN (online)	2046-5556
ISSN	0264-8725
DOI	10.1080/02648725.2023.2182546
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Article ; Online: Biodegradation of lindane (γ-Hexachlorocyclohexane) and other isomers by Bacillus subtilis strain Mz-13i

Pannu, Rochika / Kumar, Dharmender

Biocatalysis and Agricultural Biotechnology. 2023 Mar., v. 48 p.102630-

2023

Abstract: In this study, the lindane degrading bacterial isolate Mz-13i was isolated using the enrichment culture technique. The isolate was found positive for HCH utilization when screened through colorimetric assay. It was able to withstand higher concentrations ...

Abstract	In this study, the lindane degrading bacterial isolate Mz-13i was isolated using the enrichment culture technique. The isolate was found positive for HCH utilization when screened through colorimetric assay. It was able to withstand higher concentrations of lindane up to 100 ppm. Further, the bacterium was cultured on mineral salt medium (MSM) agar plates, and its physiological, morphological, and biochemical studies and molecular analysis through 16S rRNA sequencing were carried out. The 16S rRNA gene sequence of the isolate was deposited to NCBI GenBank with accession number MF599367 and classified as Bacillus subtilis strain Mz-13i through phylogenetic analysis. Gas-Chromatography Mass-Spectroscopy (GC-MS) analysis indicated the peaks for pathway-specific metabolites, viz., for γ-PCCH (7.852), benzoquinone (9.604), benzene carboxylic acid (12.608), oxalic acid (30.308) and benzene derivatives were found. During degradation, the chloride ions releases such as 0.22 mg/ml (α-HCH), 0.27 mg/ml (β-HCH), 0.48 mg/ml (γ-HCH), 0.30 mg/ml (δ-HCH). The half-life period calculated was 1.35 days for γ-HCH and 15.0 days for β-HCH. From the degradation kinetics data, the γ-isomer of HCH mediated for degradation, whereas the β-isomer was hardly degradable. The degradation for four isomers was 58.55% (α-HCH), 15% (β-HCH), 78.25 (γ-HCH), and 56% (δ-HCH).
Keywords	Bacillus subtilis ; agar ; agricultural biotechnology ; bacteria ; benzene ; benzoquinones ; biocatalysis ; biodegradation ; chlorides ; colorimetry ; enrichment culture ; gas chromatography ; half life ; lindane ; metabolites ; nucleotide sequences ; oxalic acid ; phylogeny ; Gas chromatography-mass spectroscopy (GC-MS) ; Polymerase chain reaction (PCR) ; Kinetic study ; Sphingomonas japonicum strain MTCC6362
Language	English
Dates of publication	2023-03
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	2642052-1
ISSN	1878-8181
ISSN	1878-8181
DOI	10.1016/j.bcab.2023.102630
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Characteristics and growth kinetics of biomass of Citrobacter freundii strains PYI-2 and Citrobacter portucalensis strain YPI-2 during the biodegradation of Ibuprofen.

Chopra, Sunil / Kumar, Dharmender

International microbiology : the official journal of the Spanish Society for Microbiology

2022 Volume 25, Issue 3, Page(s) 615–628

Abstract: Ibuprofen (IBU) is the third most commonly used analgesic drug in the world. It enters the water system as a result of human excretion-based wastewater discharges. Hence, it attracts the attention of environmentalists for its ecological fate and ... ...

Abstract	Ibuprofen (IBU) is the third most commonly used analgesic drug in the world. It enters the water system as a result of human excretion-based wastewater discharges. Hence, it attracts the attention of environmentalists for its ecological fate and degradation behavior. In this study, the two IBU degrading bacterial strains, Citrobacter freundii strain PYI-2 (MT039504) and Citrobacter portucalensis strain YPI-2 (MN744335), were isolated from industrial wastewater samples using an enrichment culture method, identified, and characterized. Physiological and batch culture degradation studies have indicated that these strains involved in IBU degradation and the intermediates produced during the process were analyzed. These strains degrade IBU in the batch culture. The optimum pH was reported for degradation of the PYI2 strain (6.9) and YPI2 strain (5.8), and the optimum temperatures were 42°C and 32°C, respectively. Biomass kinetic analysis of these strains was performed based on physical parameters (temperature, pH, and rpm) and confirmed by the experimental study. As indicated in the GC-MS chromatogram peaks, viz., hydroxyibuprofen, 2-(4-hydroxyphenylpropionic acid), 1,4-hydroquinone, and 2-hydroxy-1,4-quinol various intermediates compounds of degradation pathway were observed. Finally, through the GC-MS data, the metabolic pathway for degradation was predicted. In the study, it was confirmed that Citrobacter freundii strain PYI-2 and Citrobacter portucalensis strain YPI-2 exhibit metabolic potential for the biodegradation of IBU and can be further deployed in bioremediation.
MeSH term(s)	Biodegradation, Environmental ; Biomass ; Citrobacter ; Citrobacter freundii/metabolism ; Humans ; Ibuprofen/chemistry ; Ibuprofen/metabolism ; Kinetics ; Waste Water
Chemical Substances	Waste Water ; Ibuprofen (WK2XYI10QM)
Language	English
Publishing date	2022-05-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1454951-7
ISSN	1618-1905 ; 1139-6709
ISSN (online)	1618-1905
ISSN	1139-6709
DOI	10.1007/s10123-022-00248-7
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Article: A Comprehensive Survey on Pandemic Patient Monitoring System: Enabling Technologies, Opportunities, and Research Challenges.

Krishna, Charu / Kumar, Dinesh / Kushwaha, Dharmender Singh

Wireless personal communications

2023 , Page(s) 1–48

Abstract: Sporadic occurrences of transmissible diseases have severe and long-lasting effects on humankind throughout history. These outbreaks have molded the political, economic, and social aspects of human life. Pandemics have redefined some of the basic beliefs ...

Abstract	Sporadic occurrences of transmissible diseases have severe and long-lasting effects on humankind throughout history. These outbreaks have molded the political, economic, and social aspects of human life. Pandemics have redefined some of the basic beliefs of modern healthcare, pushing researchers and scientists to develop innovative solutions to be better equipped for future emergencies. Numerous attempts have been made to fight Covid-19-like pandemics using technologies such as the Internet of Things, wireless body area network, blockchain, and machine learning. Since the disease is highly contagious, novel research in patients' health monitoring system is essential for the constant monitoring of pandemic patients with minimal or no human intervention. With the ongoing pandemic of SARS-CoV-2, popularly known as Covid-19, innovations for monitoring of patients' vitals and storing them securely have risen more than ever. Analyzing the stored patients' data can further assist healthcare workers in their decision-making process. In this paper, we surveyed the research works on remote monitoring of pandemic patients admitted in hospitals or quarantined at home. First, an overview of pandemic patient monitoring is given followed by a brief introduction of enabling technologies i.e. Internet of Things, blockchain, and machine learning to implement the system. The reviewed works have been classified into three categories; remote monitoring of pandemic patients using IoT, blockchain-based storage or sharing platforms for patients' data, and processing/analyzing the stored patients' data using machine learning for prognosis and diagnosis. We also identified several open research issues to set directions for future research.
Language	English
Publishing date	2023-06-02
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	1479327-1
ISSN	1572-834X ; 0929-6212
ISSN (online)	1572-834X
ISSN	0929-6212
DOI	10.1007/s11277-023-10535-9
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Article ; Online: Dataset on modeling and optimization analysis of biodegradation of paracetamol.

Chopra, Sunil / Kumar, Dharmender

Data in brief

2020 Volume 31, Page(s) 105826

Abstract: This article contains the experimental and statistical data related to degradation of acetaminophen (paracetamol, APAP) by bacterial strains. The strains used in this study were isolated from wastewater by enrichment culture method. The optimization was ... ...

Abstract	This article contains the experimental and statistical data related to degradation of acetaminophen (paracetamol, APAP) by bacterial strains. The strains used in this study were isolated from wastewater by enrichment culture method. The optimization was important to identify the physical conditions at which the strain degraded the APAP effectively. Therefore, the Box-Behnken design (BBD) was used to know the influence of physical parameters (
Language	English
Publishing date	2020-06-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2786545-9
ISSN	2352-3409 ; 2352-3409
ISSN (online)	2352-3409
ISSN	2352-3409
DOI	10.1016/j.dib.2020.105826
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Article ; Online: Ibuprofen as an emerging organic contaminant in environment, distribution and remediation

Sunil Chopra / Dharmender Kumar

Heliyon, Vol 6, Iss 6, Pp e04087- (2020)

2020

Abstract: Pharmaceutical and personal care products (PPCPs) are the one of sub-class under emerging organic contaminants (EOCs). Ibuprofen is the world's third most consumable drug. This drug enters into our water system through human pharmaceutical use. It ... ...

Abstract	Pharmaceutical and personal care products (PPCPs) are the one of sub-class under emerging organic contaminants (EOCs). Ibuprofen is the world's third most consumable drug. This drug enters into our water system through human pharmaceutical use. It attracts the attention of environmentalist on the basis of risk associated, presence and transformation in the environment. The detection and removal are the two key area where we need to focus. The concentration of such compounds in waterbodies detected through conventional and also by the advanced methods. This review we described the available technologies including chemical, physical and biological methods, etc used the for removal of Ibuprofen. The pure culture based method, mixed culture approach and activated sludge culture approach focused and pathway of degradation of ibuprofen was deciphered by using the various methods of structure determination. The various degradation methods used for Ibuprofen are discussed. The advanced methods coupled with physical, chemical, biological, chemical methods like ozonolysis, oxidation and adsorption, nanotechnology based methods, nanocatalysis and use of nonosensors to detect the presence of small amount in waterbodies can enhance the future degradation of this drug. It is necessary to develop the new detection methods to enhance the detection of such pollutants. With the developments in new detection methods based on GC-MS//MS, HPLC, LC/MS and nanotechnology based sensors makes easier detection of these compounds which can detect even very minute amount with great sensitivity and in less time. Also, the isolation and characterization of more potent microbial strains and nano-photocatalysis will significantly increase the future degradation of such harmful compounds from the environment.
Keywords	Ibuprofen ; Emerging organic pollutants ; Bioremediation ; Degradation pathway ; Degradation intermediates ; Nano based methods ; Science (General) ; Q1-390 ; Social sciences (General) ; H1-99
Subject code	540
Language	English
Publishing date	2020-06-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Dataset on modeling and optimization analysis of biodegradation of paracetamol

Sunil Chopra / Dharmender Kumar

Data in Brief, Vol 31, Iss , Pp 105826- (2020)

2020

Abstract	This article contains the experimental and statistical data related to degradation of acetaminophen (paracetamol, APAP) by bacterial strains. The strains used in this study were isolated from wastewater by enrichment culture method. The optimization was important to identify the physical conditions at which the strain degraded the APAP effectively. Therefore, the Box-Behnken design (BBD) was used to know the influence of physical parameters (viz. pH, temperature, agitation speed, and concentration) on the degradation of APAP. The effects of the physical factor on the degradation process were investigated by a mathematical model, and this had indicated that all physical factors having some effect on the biodegradation of the APAP. Analysis of variance (ANOVA) showed that the strains DPP1, DPP3, DKP1, and DKP2 had the F-value of 12.89, 6.45, 4.58, and 5.31, respectively. This indicated, the model was significant with regression coefficient (R) value of 0.01%, 0.06%, 0.37%, and 0.18%, respectively. The experimental values, predicted data, and ANOVA analysis has suggested that the model was satisfactory.
Keywords	Acetaminophen ; Biodegradation ; Box-behnken design (BBD) ; Design expertⓇ software ; Wastewater ; Computer applications to medicine. Medical informatics ; R858-859.7 ; Science (General) ; Q1-390
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Analysis of Achievement of Radiological Parameters in Uncemented Total Hip Replacement

Narendra Singh Kushwaha / Mayank Mahendra / ARPIT SINGH / Ash Ar reza / SANJIV KUMAR / DHARMENDER KUMAR

Journal of Clinical and Diagnostic Research, Vol 17, Iss 10, Pp 01-

A Retrospective Cohort Study

2023 Volume 06

Abstract: Introduction: The Total Hip Replacement (THR) procedure is indicated to the treatment of choice in chronic refractory joint pain and some types of proximal femoral fractures. Component malalignment is a major cause of THR failure, making it crucial to ... ...

Abstract	Introduction: The Total Hip Replacement (THR) procedure is indicated to the treatment of choice in chronic refractory joint pain and some types of proximal femoral fractures. Component malalignment is a major cause of THR failure, making it crucial to position the components anatomically for long-term joint survival. Aim: To assess the radiological parameters of uncemented THR surgery. Materials and Methods: This retrospective cohort study was conducted in the Department of Orthopaedic Surgery, King’s George Medical University (KGMU), Lucknow, Uttar Pradesh, India, from June 2017 to May 2021. It included 72 patients who underwent unilateral uncemented THR for isolated hip diseases. Data was collected over the first two years and analysed. Demographic information and radiographic characteristics such as acetabular cup inclination and anteversion, femoral stem placement, vertical and horizontal centers of rotation, and limb length discrepancy were determined. Data was entered into Microsoft Excel 2018-19, and Statistical Package for the Social Sciences (SPSS) software version 28.0 was used for statistical analysis. Results: The average age of the patients was 50.75±9.1 years. Comparison of acetabular cup inclination (preoperative 41.4±3.9° and postoperative 42.2±5.1°) and anteversion (preoperative 13.1±2.96° and postoperative 14.5±3.75°) showed non significant differences following treatment with a normal hip. However, there was a significant difference in the horizontal and vertical Centers Of Rotation (COR) following treatment with a normal hip. Conclusion: Preoperative radiological characteristics of the damaged hip were significantly restored to normal anatomy and alignment following surgery.
Keywords	anteversion ; inclination ; total hip arthroplasty ; vertical offset ; Medicine ; R
Subject code	796
Language	English
Publishing date	2023-10-01T00:00:00Z
Publisher	JCDR Research and Publications Private Limited
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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