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Article ; Online: Insulin detemir: a review.

Hordern, S V M

Timely topics in medicine. Cardiovascular diseases

2007 Volume 11, Page(s) E3

Abstract: Patients with diabetes are at increased risk of mortality and morbidity from micro- and macrovascular complications. Landmark studies in type 1 and 2 diabetes have clearly shown that improved glycemic control leads to better outcomes. With the ... ...

Abstract	Patients with diabetes are at increased risk of mortality and morbidity from micro- and macrovascular complications. Landmark studies in type 1 and 2 diabetes have clearly shown that improved glycemic control leads to better outcomes. With the introduction of the General Medical Service contract, the England and Wales National Service Framework, and other schemes, there is a national drive to improve control in patients with diabetes. The treatment of diabetes was revolutionized shortly after the turn of the 20th century by the extraction and purification of insulin. Since methods to protract (i.e., prolong) the action of insulin were developed in the 1930s, little changed in this technology until the turn of this century. At this time there was renewed interest in the importance of basal insulin in controlling diabetes and thus preventing or delaying complications, and so technology advanced again. Two new basal insulin analogues have come to the market: insulin glargine, which has been widely used for some years now, and detemir. This review describes the novel method of protraction employed by insulin detemir, discusses the possible therapeutic benefits of this method of protraction, and describes the findings of studies comparing insulin detemir with other currently available basal insulin preparations. It is not the intention of this paper to be a review of all the currently available long-acting insulin analogues.
Language	English
Publishing date	2007-01-09
Publishing country	United States
Document type	Journal Article
ISSN	1579-0789
ISSN (online)	1579-0789
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Insulin detemir: a review.

Hordern, S V M

Drugs of today (Barcelona, Spain : 1998)

2006 Volume 42, Issue 8, Page(s) 505–517

Abstract: Patients with diabetes are at increased risk of mortality and morbidity from micro- and macrovascular complications (1, 2). Landmark studies in type 1 and 2 diabetes have clearly shown that improved glycemic control leads to better outcomes (3-6). With ... ...

Abstract	Patients with diabetes are at increased risk of mortality and morbidity from micro- and macrovascular complications (1, 2). Landmark studies in type 1 and 2 diabetes have clearly shown that improved glycemic control leads to better outcomes (3-6). With the introduction of the General Medical Service contract, the England and Wales National Service Framework, and other schemes, there is a national drive to improve control in patients with diabetes. The treatment of diabetes was revolutionized shortly after the turn of the 20th century by the extraction and purification of insulin. Since methods to protract (i.e., prolong) the action of insulin were developed in the 1930s, little changed in this technology until the turn of this century. At this time there was renewed interest in the importance of basal insulin in controlling diabetes and thus preventing or delaying complications, and so technology advanced again. Two new basal insulin analogues have come to the market: insulin glargine, which has been widely used for some years now, and detemir. This review describes the novel method of protraction employed by insulin detemir, discusses the possible therapeutic benefits of this method of protraction, and describes the findings of studies comparing insulin detemir with other currently available basal insulin preparations. It is not the intention of this paper to be a review of all the currently available long-acting insulin analogues.
MeSH term(s)	Diabetes Mellitus/drug therapy ; Humans ; Hypoglycemic Agents/chemistry ; Hypoglycemic Agents/pharmacokinetics ; Hypoglycemic Agents/pharmacology ; Hypoglycemic Agents/therapeutic use ; Insulin/analogs & derivatives ; Insulin/chemistry ; Insulin/pharmacokinetics ; Insulin/pharmacology ; Insulin/therapeutic use ; Insulin Detemir ; Insulin, Long-Acting ; Multicenter Studies as Topic ; Randomized Controlled Trials as Topic
Chemical Substances	Hypoglycemic Agents ; Insulin ; Insulin, Long-Acting ; Insulin Detemir (4FT78T86XV)
Language	English
Publishing date	2006-08
Publishing country	Spain
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ISSN	1699-3993
ISSN	1699-3993
DOI	10.1358/dot.2006.42.8.996567
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Article ; Online: Wastewater monitoring for detection of public health markers during the COVID-19 pandemic: Near-source monitoring of schools in England over an academic year.

PloS one

2023 Volume 18, Issue 5, Page(s) e0286259

Abstract: ... for genomic sequencing, enabling determination of the presence of SARS-CoV-2 and emergence of variant(s) contributing ...

Abstract	Background: Schools are high-risk settings for infectious disease transmission. Wastewater monitoring for infectious diseases has been used to identify and mitigate outbreaks in many near-source settings during the COVID-19 pandemic, including universities and hospitals but less is known about the technology when applied for school health protection. This study aimed to implement a wastewater surveillance system to detect SARS-CoV-2 and other public health markers from wastewater in schools in England. Methods: A total of 855 wastewater samples were collected from 16 schools (10 primary, 5 secondary and 1 post-16 and further education) over 10 months of school term time. Wastewater was analysed for SARS-CoV-2 genomic copies of N1 and E genes by RT-qPCR. A subset of wastewater samples was sent for genomic sequencing, enabling determination of the presence of SARS-CoV-2 and emergence of variant(s) contributing to COVID-19 infections within schools. In total, >280 microbial pathogens and >1200 AMR genes were screened using RT-qPCR and metagenomics to consider the utility of these additional targets to further inform on health threats within the schools. Results: We report on wastewater-based surveillance for COVID-19 within English primary, secondary and further education schools over a full academic year (October 2020 to July 2021). The highest positivity rate (80.4%) was observed in the week commencing 30th November 2020 during the emergence of the Alpha variant, indicating most schools contained people who were shedding the virus. There was high SARS-CoV-2 amplicon concentration (up to 9.2x106 GC/L) detected over the summer term (8th June - 6th July 2021) during Delta variant prevalence. The summer increase of SARS-CoV-2 in school wastewater was reflected in age-specific clinical COVID-19 cases. Alpha variant and Delta variant were identified in the wastewater by sequencing of samples collected from December to March and June to July, respectively. Lead/lag analysis between SARS-CoV-2 concentrations in school and WWTP data sets show a maximum correlation between the two-time series when school data are lagged by two weeks. Furthermore, wastewater sample enrichment coupled with metagenomic sequencing and rapid informatics enabled the detection of other clinically relevant viral and bacterial pathogens and AMR. Conclusions: Passive wastewater monitoring surveillance in schools can identify cases of COVID-19. Samples can be sequenced to monitor for emerging and current variants of concern at the resolution of school catchments. Wastewater based monitoring for SARS-CoV-2 is a useful tool for SARS-CoV-2 passive surveillance and could be applied for case identification and containment, and mitigation in schools and other congregate settings with high risks of transmission. Wastewater monitoring enables public health authorities to develop targeted prevention and education programmes for hygiene measures within undertested communities across a broad range of use cases.
MeSH term(s)	Humans ; COVID-19/epidemiology ; SARS-CoV-2/genetics ; Wastewater ; Public Health ; Pandemics ; Wastewater-Based Epidemiological Monitoring ; England/epidemiology ; RNA, Viral
Chemical Substances	Wastewater ; RNA, Viral
Language	English
Publishing date	2023-05-30
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0286259
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Article ; Online: Monitoring occurrence of SARS-CoV-2 in school populations: A wastewater-based approach.

Castro-Gutierrez, Victor / Hassard, Francis / Vu, Milan / Leitao, Rodrigo / Burczynska, Beata / Wildeboer, Dirk / Stanton, Isobel / Rahimzadeh, Shadi / Baio, Gianluca / Garelick, Hemda / Hofman, Jan / Kasprzyk-Hordern, Barbara / Kwiatkowska, Rachel / Majeed, Azeem / Priest, Sally / Grimsley, Jasmine / Lundy, Lian / Singer, Andrew C / Di Cesare, Mariachiara

PloS one

2022 Volume 17, Issue 6, Page(s) e0270168

Abstract: Clinical testing of children in schools is challenging, with economic implications limiting its frequent use as a monitoring tool of the risks assumed by children and staff during the COVID-19 pandemic. Here, a wastewater-based epidemiology approach has ... ...

Abstract	Clinical testing of children in schools is challenging, with economic implications limiting its frequent use as a monitoring tool of the risks assumed by children and staff during the COVID-19 pandemic. Here, a wastewater-based epidemiology approach has been used to monitor 16 schools (10 primary, 5 secondary and 1 post-16 and further education) in England. A total of 296 samples over 9 weeks have been analysed for N1 and E genes using qPCR methods. Of the samples returned, 47.3% were positive for one or both genes with a detection frequency in line with the respective local community. WBE offers a low cost, non-invasive approach for supplementing clinical testing and can provide longitudinal insights that are impractical with traditional clinical testing.
MeSH term(s)	COVID-19/epidemiology ; Child ; Humans ; Pandemics ; SARS-CoV-2/genetics ; Schools ; Waste Water
Chemical Substances	Waste Water
Language	English
Publishing date	2022-06-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0270168
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Article ; Online: Prevalence of pre-eclampsia and adverse pregnancy outcomes in women with pre-existing cardiomyopathy: a multi-centre retrospective cohort study.

Ormesher, Laura / Vause, Sarah / Higson, Suzanne / Roberts, Anna / Clarke, Bernard / Curtis, Stephanie / Ordonez, Victoria / Ansari, Faiza / Everett, Thomas R / Hordern, Claire / Mackillop, Lucy / Stern, Victoria / Bonnett, Tessa / Reid, Alice / Wallace, Suzanne / Oyekan, Ebruba / Douglas, Hannah / Cauldwell, Matthew / Reddy, Maya /

Palmer, Kirsten / Simpson, Maggie / Brennand, Janet / Minns, Laura / Freeman, Leisa / Murray, Sarah / Mary, Nirmala / Castleman, James / Morris, Katie R / Haslett, Elizabeth / Cassidy, Christopher / Johnstone, Edward D / Myers, Jenny E

Scientific reports

2023 Volume 13, Issue 1, Page(s) 153

Abstract: Pre-eclampsia is associated with postnatal cardiac dysfunction; however, the nature of this relationship remains uncertain. This multicentre retrospective cohort study aimed to determine the prevalence of pre-eclampsia in women with pre-existing cardiac ... ...

Abstract	Pre-eclampsia is associated with postnatal cardiac dysfunction; however, the nature of this relationship remains uncertain. This multicentre retrospective cohort study aimed to determine the prevalence of pre-eclampsia in women with pre-existing cardiac dysfunction (left ventricular ejection fraction < 55%) and explore the relationship between pregnancy outcome and pre-pregnancy cardiac phenotype. In this cohort of 282 pregnancies, pre-eclampsia prevalence was not significantly increased (4.6% [95% C.I 2.2-7.0%] vs. population prevalence of 4.6% [95% C.I. 2.7-8.2], p = 0.99); 12/13 women had concurrent obstetric/medical risk factors for pre-eclampsia. The prevalence of preterm pre-eclampsia (< 37 weeks) and fetal growth restriction (FGR) was increased (1.8% vs. 0.7%, p = 0.03; 15.2% vs. 5.5%, p < 0.001, respectively). Neither systolic nor diastolic function correlated with pregnancy outcome. Antenatal ß blockers (n = 116) were associated with lower birthweight Z score (adjusted difference - 0.31 [95% C.I. - 0.61 to - 0.01], p = 0.04). To conclude, this study demonstrated a modest increase in preterm pre-eclampsia and significant increase in FGR in women with pre-existing cardiac dysfunction. Our results do not necessarily support a causal relationship between cardiac dysfunction and pre-eclampsia, especially given the population's background risk status. The mechanism underpinning the relationship between cardiac dysfunction and FGR merits further research but could be influenced by concomitant ß blocker use.
MeSH term(s)	Humans ; Pregnancy ; Female ; Pre-Eclampsia/epidemiology ; Pregnancy Outcome ; Retrospective Studies ; Stroke Volume ; Ventricular Function, Left ; Fetal Growth Retardation/epidemiology ; Cardiomyopathies/complications ; Cardiomyopathies/epidemiology ; Heart Diseases
Language	English
Publishing date	2023-01-04
Publishing country	England
Document type	Multicenter Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-26606-z
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Article: Insulin detemir, does a new century bring a better basal insulin?

Hordern, S V M / Russell-Jones, D L

International journal of clinical practice

2005 Volume 59, Issue 6, Page(s) 730–739

Abstract: The treatment of diabetes was revolutionised shortly after the turn of the twentieth century by the extraction and purification of insulin. Methods to protract (i.e. prolong) the action of insulin were developed in the 1930s; little changed in the ... ...

Abstract	The treatment of diabetes was revolutionised shortly after the turn of the twentieth century by the extraction and purification of insulin. Methods to protract (i.e. prolong) the action of insulin were developed in the 1930s; little changed in the technology of insulin protraction until the turn of this century when, with renewed interest in the importance of basal insulin in controlling diabetes and thus preventing or delaying complications, technology advanced again. Two new long-acting insulin analogues have come to the market; some may be familiar with insulin glargine, which has been widely used for some years now. This review attempts to describe the novel method of protraction that insulin detemir (launched last summer) employs by albumin binding, to discuss the possible therapeutic benefits of this method of protraction and to describe the findings of studies comparing insulin detemir with other currently available long-acting insulin preparations. The intention of this article is not to review all of the currently available long-acting insulin analogues.
MeSH term(s)	Delayed-Action Preparations ; Diabetes Mellitus/drug therapy ; Glucose/metabolism ; Humans ; Hypoglycemia/drug therapy ; Hypoglycemic Agents/therapeutic use ; Insulin/analogs & derivatives ; Insulin/therapeutic use ; Insulin Detemir ; Insulin, Long-Acting/analogs & derivatives ; Insulin, Long-Acting/therapeutic use
Chemical Substances	Delayed-Action Preparations ; Hypoglycemic Agents ; Insulin ; Insulin, Long-Acting ; Insulin Detemir (4FT78T86XV) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2005-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	1386246-7
ISSN	1742-1241 ; 1368-5031
ISSN (online)	1742-1241
ISSN	1368-5031
DOI	10.1111/j.1368-5031.2005.00544.x
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Article ; Online: The role of analytical chemistry in exposure science: Focus on the aquatic environment.

Hernández, F / Bakker, J / Bijlsma, L / de Boer, J / Botero-Coy, A M / Bruinen de Bruin, Y / Fischer, S / Hollender, J / Kasprzyk-Hordern, B / Lamoree, M / López, F J / Laak, T L Ter / van Leerdam, J A / Sancho, J V / Schymanski, E L / de Voogt, P / Hogendoorn, E A

Chemosphere

2019 Volume 222, Page(s) 564–583

Abstract: Exposure science, in its broadest sense, studies the interactions between stressors (chemical, biological, and physical agents) and receptors (e.g. humans and other living organisms, and non-living items like buildings), together with the associated ... ...

Abstract	Exposure science, in its broadest sense, studies the interactions between stressors (chemical, biological, and physical agents) and receptors (e.g. humans and other living organisms, and non-living items like buildings), together with the associated pathways and processes potentially leading to negative effects on human health and the environment. The aquatic environment may contain thousands of compounds, many of them still unknown, that can pose a risk to ecosystems and human health. Due to the unquestionable importance of the aquatic environment, one of the main challenges in the field of exposure science is the comprehensive characterization and evaluation of complex environmental mixtures beyond the classical/priority contaminants to new emerging contaminants. The role of advanced analytical chemistry to identify and quantify potential chemical risks, that might cause adverse effects to the aquatic environment, is essential. In this paper, we present the strategies and tools that analytical chemistry has nowadays, focused on chromatography hyphenated to (high-resolution) mass spectrometry because of its relevance in this field. Key issues, such as the application of effect direct analysis to reduce the complexity of the sample, the investigation of the huge number of transformation/degradation products that may be present in the aquatic environment, the analysis of urban wastewater as a source of valuable information on our lifestyle and substances we consumed and/or are exposed to, or the monitoring of drinking water, are discussed in this article. The trends and perspectives for the next few years are also highlighted, when it is expected that new developments and tools will allow a better knowledge of chemical composition in the aquatic environment. This will help regulatory authorities to protect water bodies and to advance towards improved regulations that enable practical and efficient abatements for environmental and public health protection.
MeSH term(s)	Chemistry Techniques, Analytical ; Ecosystem ; Environmental Exposure/analysis ; Environmental Monitoring ; Humans
Language	English
Publishing date	2019-01-23
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2019.01.118
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Article: Enantioselective fractionation of fluoroquinolones in the aqueous environment using chiral liquid chromatography coupled with tandem mass spectrometry

Castrignanò, Erika / Andrew M. Kannan / Barbara Kasprzyk-Hordern / Edward J. Feil

Chemosphere. 2018 Sept., v. 206

2018

Abstract: ... racemic in upstream waters but it was enriched with S-(−)-enantiomer in wastewater and in receiving waters ... This could be due to the fact that ofloxacin can be used both as a racemate and as a S-(−)-enantiomer ...

Abstract	This paper aims to examine the multiresidue enantiomeric profiling of (fluoro)quinolones and their metabolites in solid and liquid environmental matrices using chiral HPLC-MS/MS method and a CHIRALCEL® OZ-RH column. Simultaneous chiral separation was obtained for chiral ofloxacin and its main metabolites ofloxacin-N-oxide and desmethyl-ofloxacin; moxifloxacin; the prodrug prulifloxacin and its active compound ulifloxacin; flumequine; nadifloxacin and R-(+)-besifloxacin. Achiral antibiotics (ciprofloxacin, norfloxacin and nalidixic acid) were also included in the method to enable the analysis of all targeted quinolones within one analytical run. Satisfactory enantiomeric resolution (Rs ≥ 1) was obtained for five out of eight chiral drugs enabling quantitative analysis. The overall performance of the method was satisfactory with a method precision <20%, relative recoveries >70% for most of the analytes and method detection limits (MDL) at low ng L−1 levels (0.1 < MDL (ng L−1)< 6.4, 0.1 < MDL (ng L−1)< 6.6 and 0.1 < MDL (ng L−1)< 7.0 in influent, effluent and river waters for 83% compounds, 0.01 < MDL (ng g−1)< 4.9 in solids for 91% compounds). Enantiomeric profiling from a week-long monitoring campaign in the UK showed that (±)-ofloxacin was found to be racemic in upstream waters but it was enriched with S-(−)-enantiomer in wastewater and in receiving waters. This could be due to the fact that ofloxacin can be used both as a racemate and as a S-(−)-enantiomer. Its consumption was further confirmed by the chiral signature of the investigated ofloxacin metabolites. As a result, alterations in the enantiomeric composition of antibiotics could influence not only their activity and toxicity in the environment, but also could induce changes in the microbial communities constantly exposed to them.
Keywords	active ingredients ; antibiotics ; ciprofloxacin ; drugs ; enantioselectivity ; flumequine ; fractionation ; liquid chromatography ; liquids ; metabolites ; microbial communities ; monitoring ; moxifloxacin ; nalidixic acid ; norfloxacin ; ofloxacin ; quantitative analysis ; river water ; tandem mass spectrometry ; toxicity ; wastewater ; United Kingdom
Language	English
Dates of publication	2018-09
Size	p. 376-386.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2018.05.005
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Article: Wastewater Analysis for Community-Wide Drugs Use Assessment.

Ort, Christoph / Bijlsma, Lubertus / Castiglioni, Sara / Covaci, Adrian / de Voogt, Pim / Emke, Erik / Hernández, Félix / Reid, Malcolm / van Nuijs, Alexander L N / Thomas, Kevin V / Kasprzyk-Hordern, Barbara

Handbook of experimental pharmacology

2018 Volume 252, Page(s) 543–566

Abstract: Wastewater-based epidemiology (WBE) complements existing epidemiology-based estimation techniques and provides objective, evidence-based estimates of illicit drug use. After consumption, biomarkers - drugs and their metabolites - excreted to toilets and ... ...

Abstract	Wastewater-based epidemiology (WBE) complements existing epidemiology-based estimation techniques and provides objective, evidence-based estimates of illicit drug use. After consumption, biomarkers - drugs and their metabolites - excreted to toilets and flushed into urban sewer networks can be measured in raw wastewater samples. The quantified loads can serve as an estimate for the collective consumption of all people contributing to the wastewater sample. This transdisciplinary approach, further explained in this chapter, has developed, matured and is now established for monitoring substances such as cocaine and amphetamine-type stimulants. Research currently underway is refining WBE to new applications including new psychoactive substances (NPS).
MeSH term(s)	Amphetamine/analysis ; Central Nervous System Stimulants/analysis ; Cocaine/analysis ; Humans ; Street Drugs/analysis ; Substance-Related Disorders/epidemiology ; Waste Water/analysis
Chemical Substances	Central Nervous System Stimulants ; Street Drugs ; Waste Water ; Amphetamine (CK833KGX7E) ; Cocaine (I5Y540LHVR)
Language	English
Publishing date	2018-06-13
Publishing country	Germany
Document type	Journal Article
ISSN	0171-2004
ISSN	0171-2004
DOI	10.1007/164_2018_111
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Article ; Online: A novel DNA biosensor using a ferrocenyl intercalator applied to the potential detection of human population biomarkers in wastewater.

Yang, Zhugen / Anglès d'Auriac, Marc / Goggins, Sean / Kasprzyk-Hordern, Barbara / Thomas, Kevin V / Frost, Christopher G / Estrela, Pedro

Environmental science & technology

2015 Volume 49, Issue 9, Page(s) 5609–5617

Abstract: A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol ... ...

Abstract	A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol to control the surface density of the ssDNA probe, and hybridized with complementary DNA. The binding of the Fc intercalator to dsDNA was measured by differential pulse voltammetry. This new biosensor was optimized to allow the detection of single base pair mismatched sequences, able to detect as low as 10 pM target ssDNA with a dynamic range from 10 pM to 100 nM. DNA extracted from wastewater was analyzed by quantitative polymerase chain reaction targeting human-specific mitochondrial DNA (mtDNA). The aim of this approach is to enable the analysis of population biomarkers in wastewater for the evaluation of public health using wastewater-based epidemiology (WBE). The E-DNA biosensor was employed to detect human-specific mtDNA from wastewater before and after PCR amplification. The results demonstrate the feasibility of detecting human DNA biomarkers in wastewater using the developed biosensor, which may allow the further development of DNA population biomarkers for public health using WBE.
MeSH term(s)	Biomarkers/analysis ; Biosensing Techniques/methods ; DNA/analysis ; DNA/genetics ; DNA, Complementary/analysis ; DNA, Mitochondrial/analysis ; Electrochemical Techniques ; Electrodes ; Gold/analysis ; Humans ; Intercalating Agents/chemistry ; Waste Water/analysis
Chemical Substances	Biomarkers ; DNA, Complementary ; DNA, Mitochondrial ; Intercalating Agents ; Waste Water ; Gold (7440-57-5) ; DNA (9007-49-2)
Language	English
Publishing date	2015-05-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1520-5851
ISSN (online)	1520-5851
DOI	10.1021/acs.est.5b00637
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