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  1. Article: Comparison of the intestinal microbiota composition and function in healthy and diseased Yunlong Grouper.

    Ma, Chao / Chen, Chunxiu / Jia, Lei / He, Xiaoxu / Zhang, Bo

    AMB Express

    2019  Volume 9, Issue 1, Page(s) 187

    Abstract: ... analysis of intestinal microbiota in Yunlong Grouper (Epinephelus moara♀ × Epinephelus lanceolatus♂ ... of predominant phyla like the Proteobacteria were upregulated in the diseased Yunlong Grouper. At the genus level ... to designate a healthy status in Yunlong Grouper. The environmental bacterial community influenced composition ...

    Abstract Maintaining stabilization of the intestinal microbiota is important in preventing bacterial diseases in cultured fish. At present, there have been no reports on the composition and functional analysis of intestinal microbiota in Yunlong Grouper (Epinephelus moara♀ × Epinephelus lanceolatus♂). In this study we analyzed and compared the intestinal microbiota composition of healthy and diseased pond-reared fish to discern the functional profile of a healthy status. The richness and diversity of the intestinal microbiota did not differ significantly between diseased and healthy fish, yet the abundance of predominant phyla like the Proteobacteria were upregulated in the diseased Yunlong Grouper. At the genus level, a significant reduction of Cetobacterium was observed in the intestinal tracts of diseased fish, as Pseudomonas became the most dominant bacterium. To compare the intestinal microorganism abundances between the two health groups of fish, we first screened the gut bacteria and discerned 4 phyla and 12 genera to designate a healthy status in Yunlong Grouper. The environmental bacterial community influenced composition of the intestinal microbiota in Yunlong Grouper, and the intestinal microbiota of diseased fish was more susceptible to the influence of the culture water. In addition, the prediction of functional genes by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) indicated that the intestinal microbiota of Yunlong Grouper is related mainly to the terms "metabolism, environmental information processing, genetic information processing, human diseases, and cellular processing; moreover, the functions of the intestinal microbiota differed between the different health states of this fish. The overall results indicate that the occurrence of disease can affect the composition and function of the intestinal microbiota in a cultured fish.
    Language English
    Publishing date 2019-11-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2621432-5
    ISSN 2191-0855
    ISSN 2191-0855
    DOI 10.1186/s13568-019-0913-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Construction of high-density linkage maps and QTL mapping for growth-related traits in F1 hybrid Yunlong grouper (Epinephelus moara♀ × E. lanceolatus♂)

    Liu, Yang / Tian, Yongsheng / Wang, Linna / Li, Zhentong / Zhang, Jingjing / Wu, Yuping / Chen, Shuai / Li, Linlin / Li, Wensheng / Ma, Wenhui / Lin, Haowei / Wang, Qingbin

    Aquaculture. 2022 Feb. 15, v. 548

    2022  

    Abstract: ... we report the first high-density linkage map of Yunlong grouper (Epinephelus moara♀ × E. lanceolatus♂ ... analyses of the heterosis of some growth traits, thereby facilitating MAS in Yunlong grouper. ...

    Abstract Owing to the increasing market demand and emerging grouper aquaculture industry, close attention has been paid to genetic breeding for growth traits in grouper. The construction of linkage maps for quantitative trait loci (QTL) analyses is an essential step for marker-assisted selection (MAS) programs. In this study, we report the first high-density linkage map of Yunlong grouper (Epinephelus moara♀ × E. lanceolatus♂) constructed by sequencing 118 F1 progenies in a full-sib family using restriction site associated DNA (RAD) technology. For the female and male maps, 12,307 and 12,340 single nucleotide polymorphism (SNP) markers were anchored onto 24 linkage groups, spanning 1909.37 and 1933.61 cM, respectively, with an average marker interval of 0.16 cM. QTL mapping revealed 39 significant QTLs for three growth traits. Candidate genes participating in ubiquitin modification, cell adhesion, G-protein coupled receptors, cell proliferation, and calcium transport were identified from these QTLs, including itgb1, col1a1, pdgfc, bmpr1a, pcdhgc5, and cacna1b. The results of this study provide a foundation for genomic and genetic studies and further analyses of the heterosis of some growth traits, thereby facilitating MAS in Yunlong grouper.
    Keywords DNA ; Epinephelus ; G-proteins ; aquaculture industry ; calcium ; cell adhesion ; cell proliferation ; chromosome mapping ; females ; grouper ; males ; marker-assisted selection ; quantitative traits ; single nucleotide polymorphism ; supply balance ; ubiquitin
    Language English
    Dates of publication 2022-0215
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2021.737698
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Comparative transcriptomics analyses and revealing candidate networks and genes involved in lordosis of the Yunlong grouper (Epinephelus moara ♀ × Epinephelus lanceolatus ♂)

    Li, Zhentong / Tian, Yongsheng / Wang, Linna / Li, Ziqi / Chen, Shuai / Li, Linlin / Liu, Yang / Li, Wensheng / Pang, Zunfang / Ma, Wenhui / Wang, Qingbin / Li, Bo / Zhai, Jieming

    Aquaculture. 2022 Mar. 15, v. 550

    2022  

    Abstract: ... of Yunlong grouper. A total of 87,888 unigenes were assembled with lengths that varied from 201 to 28,922 bp ...

    Abstract Grouper is an economically important fish in China. However, it exhibits a high frequency of skeletal abnormalities, particularly vertebral deformities. The molecular mechanisms underlying fish vertebral deformities are still poorly understood. In this study, a HiSeq™ 4000 platform (Illumina) was used to analyze the transcriptomic profiles of the brain, pituitary, and vertebrae from normal fish (NF) and fish with lordosis (LF) of Yunlong grouper. A total of 87,888 unigenes were assembled with lengths that varied from 201 to 28,922 bp and a N50 length of 2670 bp. A total of 36,268 unigenes were functionally annotated by BLAST alignments. A total of 2875 significantly differentially expressed genes (DEGs) were identified between the NF group and the LF group, including 706 upregulated unigenes and 2169 downregulated unigenes in LF. GO and KEGG pathway enrichment analyses showed that DNA binding, transmembrane receptor activity, cytokine receptor interaction, neuroactive ligand-receptor interaction, calcium signaling pathway and ECM-receptor interaction HIF-1 signaling pathway, and mineral absorption may be involved in the formation of vertebral deformities. Furthermore, weighted gene co-expression network analyses, including three modules (turquoise, yellow, and blue), significantly positively corrected with vertebral deformities. A network map that included these three modules enabled the identification of a series of hub genes, including claudin-22-like (cldn22), fibronectin type III domain-containing protein 1 isoform X2 (fndc1l2), E3 ubiquitin-protein ligase NRDP1-like (rnf41), and Catenin alpha-2 (ctnna1). We found that the levels of most genes in the blue module were closely related to the expression of parvalbumin, thymic CPV3-like isoform X2 (ocm), platelet glycoprotein Ib alpha chain (gp1ba), and matrix metalloproteinase-9 (mmp9), suggesting that this module is associated with skeletal development. Some uncharacterized genes associated with known bone-related genes, including Unigene0067643, Unigene0056862, and Unigene0059867, were detected by a weighted gene co-expression network analysis. A detailed functional investigation of these networks and genes will further improve our understanding of the molecular mechanisms that underlie the formation of lordosis in fish.
    Keywords DNA ; Epinephelus lanceolatus ; absorption ; aquaculture ; brain ; calcium ; cytokine receptors ; fibronectins ; gelatinase B ; gene expression regulation ; glycoproteins ; grouper ; skeletal development ; transcriptomics ; ubiquitin-protein ligase ; unigenes ; China
    Language English
    Dates of publication 2022-0315
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 185380-6
    ISSN 0044-8486 ; 0044-8516
    ISSN 0044-8486 ; 0044-8516
    DOI 10.1016/j.aquaculture.2021.737866
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: The green lacewing genus

    Ma, Yunlong

    ZooKeys

    2022  Volume 1106, Page(s) 57–66

    Abstract: A new species, ...

    Abstract A new species,
    Language English
    Publishing date 2022-06-16
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1106.83229
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Notes on the green lacewing subgenus Chrysopidia (

    Ma, Yunlong

    ZooKeys

    2022  Volume 1106, Page(s) 67–80

    Abstract: A taxonomic study of the green lacewing subgenus Chrysopidia ( ...

    Abstract A taxonomic study of the green lacewing subgenus Chrysopidia (
    Language English
    Publishing date 2022-06-16
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1106.83232
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The green lacewing genus Anachrysa Hölzel, 1973 (Neuroptera, Chrysopidae)

    Yunlong Ma

    ZooKeys, Vol 1106, Iss , Pp 57-

    a new species, two new combinations, and updated key to species

    2022  Volume 66

    Abstract: A new species, Anachrysa adamsi sp. nov., from Yunnan, China is described. Two new combinations are proposed, namely Anachrysa alviolata (Yang & Yang, 1990), comb. nov. and Anachrysa triangularis (Yang & Wang, 1994), comb. nov. An updated key to species ... ...

    Abstract A new species, Anachrysa adamsi sp. nov., from Yunnan, China is described. Two new combinations are proposed, namely Anachrysa alviolata (Yang & Yang, 1990), comb. nov. and Anachrysa triangularis (Yang & Wang, 1994), comb. nov. An updated key to species is also provided.
    Keywords Zoology ; QL1-991
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Pensoft Publishers
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Notes on the green lacewing subgenus Chrysopidia (s. str.) Navás, 1910 (Neuroptera, Chrysopidae), with description of a new species from China

    Yunlong Ma

    ZooKeys, Vol 1106, Iss , Pp 67-

    2022  Volume 80

    Abstract: A taxonomic study of the green lacewing subgenus Chrysopidia (s. str.) from China is presented. Based on the examination of type specimens of the genus reported by previous Chinese scholars and the line drawings of Chrysopidia manipurensis provided by ... ...

    Abstract A taxonomic study of the green lacewing subgenus Chrysopidia (s. str.) from China is presented. Based on the examination of type specimens of the genus reported by previous Chinese scholars and the line drawings of Chrysopidia manipurensis provided by Ghosh (1990), I proposed five new combinations: Apertochrysa platypa (Yang & Yang, 1990), comb. nov., Apertochrysa yangi (Yang, 1997), comb. nov., Apertochrysa shennongana (Yang & Wang, 1990), comb. nov., Apertochrysa zhaoi (Yang & Wang, 1990), comb. nov., and Apertochrysa manipurensis (Ghosh, 1990), comb. nov. As for Chrysopidia sinica Yang & Wang, 1990, I still treat it as a valid species until supplementary specimens are available because the gonarcal complex of the type specimen is missing. A new species Chrysopidia (Chrysopidia) tjederi sp. nov. is also described based on new materials. A key to all known species of this subgenus is also provided.
    Keywords Zoology ; QL1-991
    Subject code 590
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher Pensoft Publishers
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: The green lacewing genus Anachrysa Hölzel, 1973 (Neuroptera, Chrysopidae): a new species, two new combinations, and updated key to species

    Ma, Yunlong

    ZooKeys. 2022 June 16, v. 1106 p.57-66

    2022  

    Abstract: AbstractA new species, Anachrysa adamsi sp. nov., from Yunnan, China is described. Two new combinations are proposed, namely Anachrysa alviolata (Yang & Yang, 1990), comb. nov. and Anachrysa triangularis (Yang & Wang, 1994), comb. nov. An updated key to ...

    Abstract AbstractA new species, Anachrysa adamsi sp. nov., from Yunnan, China is described. Two new combinations are proposed, namely Anachrysa alviolata (Yang & Yang, 1990), comb. nov. and Anachrysa triangularis (Yang & Wang, 1994), comb. nov. An updated key to species is also provided.
    Keywords Chrysopidae ; new species ; taxonomic keys ; China ; Key ; new combinations
    Language English
    Dates of publication 2022-0616
    Size p. 57-66.
    Publishing place Pensoft Publishers
    Document type Article ; Online
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1106.83229
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Notes on the green lacewing subgenus Chrysopidia (s. str.) Navás, 1910 (Neuroptera, Chrysopidae), with description of a new species from China

    Ma, Yunlong

    ZooKeys. 2022 June 16, v. 1106 p.67-80

    2022  

    Abstract: AbstractA taxonomic study of the green lacewing subgenus Chrysopidia (s. str.) from China is presented. Based on the examination of type specimens of the genus reported by previous Chinese scholars and the line drawings of Chrysopidia manipurensis ... ...

    Abstract AbstractA taxonomic study of the green lacewing subgenus Chrysopidia (s. str.) from China is presented. Based on the examination of type specimens of the genus reported by previous Chinese scholars and the line drawings of Chrysopidia manipurensis provided by Ghosh (1990), I proposed five new combinations: Apertochrysa platypa (Yang & Yang, 1990), comb. nov., Apertochrysa yangi (Yang, 1997), comb. nov., Apertochrysa shennongana (Yang & Wang, 1990), comb. nov., Apertochrysa zhaoi (Yang & Wang, 1990), comb. nov., and Apertochrysa manipurensis (Ghosh, 1990), comb. nov. As for Chrysopidia sinica Yang & Wang, 1990, I still treat it as a valid species until supplementary specimens are available because the gonarcal complex of the type specimen is missing. A new species Chrysopidia (Chrysopidia) tjederisp. nov. is also described based on new materials. A key to all known species of this subgenus is also provided.
    Keywords Chrysopidae ; new species ; taxonomic keys ; China ; Key ; taxonomic study
    Language English
    Dates of publication 2022-0616
    Size p. 67-80.
    Publishing place Pensoft Publishers
    Document type Article ; Online
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1106.83232
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: A high-resolution time-frequency analysis technique based on bi-directional squeezing and its application in fault diagnosis of rotating machinery.

    Ma, Yunlong / Yu, Gang / Lin, Tianran / Sun, Mingxu

    ISA transactions

    2024  Volume 147, Page(s) 382–402

    Abstract: Most of the signals in the field of industrial engineering are nonstationary signals, and the accurate description of the time-frequency (TF) characteristics of nonstationary signals is important for the advancement of industrial engineering. ... ...

    Abstract Most of the signals in the field of industrial engineering are nonstationary signals, and the accurate description of the time-frequency (TF) characteristics of nonstationary signals is important for the advancement of industrial engineering. Instantaneous frequency (IF) and group delay (GD) are common TF features used to describe nonstationary signals. Time-reassigned synchrosqueezing transform and synchrosqueezing transform are two TF analysis tools that can accurately characterize the GD and IF of nonstationary signals by squeezing the TF coefficients in the time direction and frequency direction, respectively. However, it is difficult for such two techniques to accurately characterize IF and GD simultaneously. A novel method called time-frequency squeezing transform is introduced in this paper to conquer this drawback. The technique first uses the short-time Fourier transform to calculate the time-frequency representation (TFR) of a signal, and then divides the TFR into two parts according to a chirp rate estimator. The subdivided TFR parts are then squeezed in the frequency and time directions to accurately characterize the IF and GD, respectively, and the two squeezed results are added to form a high-resolution result. The effectiveness of the proposed technique is demonstrated with numerical and experimental signals.
    Language English
    Publishing date 2024-02-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2012746-7
    ISSN 1879-2022 ; 0019-0578
    ISSN (online) 1879-2022
    ISSN 0019-0578
    DOI 10.1016/j.isatra.2024.01.025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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