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  1. Article ; Online: Brain as a case example of evaluating system's networkness: Comment on "Does the brain behave like a (complex) network? I. Dynamics" by David Papo & Javier M. Buldú.

    Korhonen, Onerva

    Physics of life reviews

    2024  Volume 49, Page(s) 15–16

    Language English
    Publishing date 2024-03-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2148883-6
    ISSN 1873-1457 ; 1571-0645
    ISSN (online) 1873-1457
    ISSN 1571-0645
    DOI 10.1016/j.plrev.2024.03.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Oldies, but goldies-preserved morphology and stability of antigenic determinants in decades-old cryosections of human m. vastus lateralis.

    Hutz, Bettina / Degens, Hans / Korhonen, Marko T

    Journal of anatomy

    2024  Volume 244, Issue 5, Page(s) 882–886

    Abstract: ... are well preserved in up to 18-year-stored unfixed and unstained cryosections of human m ...

    Abstract Fibre typing by immunohistochemistry on cryosections from human skeletal muscle biopsies is an essential tool in the diagnosis and research of muscular diseases, ageing, and responses to exercise training and disuse. Preserving a good quality in these frozen specimens can be challenging especially if they are stored for longer periods before histological processing, which is often the case in studies with a large number of test subjects and/or repeated sampling separated by multiple years. We demonstrate in this article that both, the morphology and reactivity of epitopes to myosin heavy chain isoforms and dystrophin are well preserved in up to 18-year-stored unfixed and unstained cryosections of human m. vastus lateralis (n = 241). Any variation in staining intensity between samples was unrelated to the age of the biopsy donor or the storage period of the unstained cryosections, and in all cases, the obtained images were appropriate for image analysis, such as the determination of the fibre type composition and the fibre cross-sectional area, and quantitative analysis of muscle capillarisation.
    MeSH term(s) Humans ; Muscle Fibers, Skeletal/physiology ; Quadriceps Muscle ; Epitopes ; Myosin Heavy Chains ; Aging ; Muscle, Skeletal/physiology
    Chemical Substances Epitopes ; Myosin Heavy Chains (EC 3.6.4.1)
    Language English
    Publishing date 2024-01-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 2955-5
    ISSN 1469-7580 ; 0021-8782
    ISSN (online) 1469-7580
    ISSN 0021-8782
    DOI 10.1111/joa.14003
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  3. Article ; Online: MKP-1 promotes anti-inflammatory M(IL-4/IL-13) macrophage phenotype and mediates the anti-inflammatory effects of glucocorticoids.

    Pemmari, Antti / Paukkeri, Erja-Leena / Hämäläinen, Mari / Leppänen, Tiina / Korhonen, Riku / Moilanen, Eeva

    Basic & clinical pharmacology & toxicology

    2018  Volume 124, Issue 4, Page(s) 404–415

    Abstract: Macrophage polarization refers to the ability of these cells to adopt different functional phenotypes according to their environment. Mitogen-activated protein kinase phosphatase-1 (MKP-1) is known to regulate the classical lipopolysaccharide (LPS)- ... ...

    Abstract Macrophage polarization refers to the ability of these cells to adopt different functional phenotypes according to their environment. Mitogen-activated protein kinase phosphatase-1 (MKP-1) is known to regulate the classical lipopolysaccharide (LPS)-induced pro-inflammatory macrophage activation and the inflammatory response. Here, we investigated the effects of MKP-1 on the anti-inflammatory and healing-promoting macrophage phenotype induced by cytokines IL-4 and IL-13 and examined the potential mediator role of MKP-1 in glucocorticoid effects on the two macrophage phenotypes. In MKP-1-deficient macrophages treated with IL-4 and IL-13 to induce the anti-inflammatory phenotype, the expression of phenotypic markers arginase 1, Ym-1 and FGF2 was reduced as compared to wild-type cells. In contrast, LPS-induced expression of the pro-inflammatory factors IL-6 and iNOS was significantly higher in MKP-1-deficient macrophages. Dexamethasone suppressed the pro-inflammatory phenotype and enhanced the anti-inflammatory phenotype. Interestingly, both of these glucocorticoid effects were attenuated in macrophages from MKP-1-deficient mice. Accordingly, dexamethasone increased MKP-1 expression in both LPS- and IL4+13-treated wild-type cells. In conclusion, the findings support MKP-1 as an endogenous mechanism able to shift macrophage activation from the classical pro-inflammatory state towards the anti-inflammatory and healing-promoting phenotype. In addition, MKP-1 was found to mediate the anti-inflammatory effects of dexamethasone in a dualistic manner: by suppressing the pro-inflammatory macrophage activation and by enhancing the healing-promoting macrophage phenotype.
    MeSH term(s) Animals ; Anti-Inflammatory Agents/pharmacology ; Dexamethasone/pharmacology ; Dual Specificity Phosphatase 1/genetics ; Dual Specificity Phosphatase 1/metabolism ; Glucocorticoids/pharmacology ; Interleukin-13/metabolism ; Interleukin-4/metabolism ; Lipopolysaccharides/toxicity ; Macrophage Activation/drug effects ; Macrophages/drug effects ; Macrophages/pathology ; Macrophages, Peritoneal/drug effects ; Macrophages, Peritoneal/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Phenotype
    Chemical Substances Anti-Inflammatory Agents ; Glucocorticoids ; Interleukin-13 ; Lipopolysaccharides ; Interleukin-4 (207137-56-2) ; Dexamethasone (7S5I7G3JQL) ; Dual Specificity Phosphatase 1 (EC 3.1.3.48) ; Dusp1 protein, mouse (EC 3.1.3.48)
    Language English
    Publishing date 2018-12-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2134679-3
    ISSN 1742-7843 ; 1742-7835
    ISSN (online) 1742-7843
    ISSN 1742-7835
    DOI 10.1111/bcpt.13163
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  4. Article ; Online: Design and Development Process of a Youth Depression Screening m-Health Application for Primary Health Care Workers in South Africa and Zambia: An Overview of the MEGA Project.

    Lahti, Mari / Groen, Gunter / Mwape, Lonia / Korhonen, Joonas / Breet, Elsie / Chapima, Fabian / Coetzee, Marita / Ellilä, Heikki / Jansen, Ronelle / Jonker, Deporah / Jörns-Presentati, Astrid / Mbanga, Ireen / Mukwato, Patrcicia / Mundenda, John / Mutagubya, Joseph / Janse van Rensburg-Bonthuyzen, Ega / Seedat, Soraya / Stein, Dan J / Suliman, Sharain /
    Sukwa, Thomas / Turunen, Timo-J / Valtins, Karlis / van den Heuvel, Leigh / Wahila, Ruth / Grobler, Gerhard

    Issues in mental health nursing

    2019  Volume 41, Issue 1, Page(s) 24–30

    Abstract: ... and evaluate a tiered education and training program in the use of the m-health application ... In the final phase, we will evaluate the acceptability and feasibility of the m-health application in PHC ... by developing and implementing a locally relevant m-health application. ...

    Abstract Literature indicates a high prevalence and burden of mental illness in youths world-wide, which may be even higher in low- and middle-income countries (LMIC), such as South Africa and Zambia. Additionally, there is a lack of knowledge regarding youth depression amongst many primary health care (PHC) practitioners. The principal goal of the MEGA project is to provide youth with better access to mental health services and appropriate care, by developing a mental health screening mobile application tool to be used in PHC settings in South Africa and Zambia. In this study, we will use a mixed methods multi-center study design. In phase one, we will investigate the mental health literacy of PHC practitioners to identify areas in need of development. Based on the needs identified, we will develop and test a mobile health application to screen for common youth mental health problems in phase two. In phase three, we will implement and evaluate a tiered education and training program in the use of the m-health application. In the final phase, we will evaluate the acceptability and feasibility of the m-health application in PHC centres across South Africa and Zambia. Evidence suggests that PHC practitioners should routinely consider mental illness when assessing youth. However, common psychiatric disorders remain largely undetected and untreated in PHC settings. By identifying limitations in PHC workers knowledge with regard to youth mental health, we aspire to improve the depression care provided to youth in Southern Africa and Zambia by developing and implementing a locally relevant m-health application.
    MeSH term(s) Adolescent ; Depression/diagnosis ; Developing Countries ; Feasibility Studies ; Health Personnel ; Humans ; Mental Health Services ; Mobile Applications ; Primary Health Care ; South Africa ; Telemedicine ; Zambia
    Language English
    Publishing date 2019-06-21
    Publishing country England
    Document type Journal Article ; Multicenter Study
    ZDB-ID 603180-8
    ISSN 1096-4673 ; 0161-2840
    ISSN (online) 1096-4673
    ISSN 0161-2840
    DOI 10.1080/01612840.2019.1604919
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  5. Article ; Online: Sparse weightings for collapsing inverse solutions to cortical parcellations optimize M/EEG source reconstruction accuracy.

    Korhonen, Onerva / Palva, Satu / Palva, J Matias

    Journal of neuroscience methods

    2014  Volume 226, Page(s) 147–160

    Abstract: Background: Source-reconstructed magneto- and electroencephalography (M/EEG) are promising tools ... comparable to magnetic resonance imaging (MRI), M/EEG source data can be collapsed into a cortical ... Conclusions: The optimized operator is suitable for collapsing real source-reconstructed M/EEG data ...

    Abstract Background: Source-reconstructed magneto- and electroencephalography (M/EEG) are promising tools for investigating the human functional connectome. To reduce data, decrease noise, and obtain results directly comparable to magnetic resonance imaging (MRI), M/EEG source data can be collapsed into a cortical parcellation. For most collapsing approaches, however, it remains unclear if collapsed parcel time series accurately represent the coherent source dynamics within each parcel.
    New method: We introduce a collapse-weighting-operator optimization approach that maximizes parcel fidelity, i.e., the phase correlation between original source dynamics and collapsed parcel time series, and thereby the accuracy with which the source dynamics are retained in forward and inverse modeling.
    Results: The sparse, optimized weighting operator increased parcel fidelity 57-73% and true positive rate of interaction mapping from 0.33 to 0.84 in comparison to a non-sparse weighting approach. These improvements were robust for variable source topographies and parcellation resolutions. Critically, in real inverse-modeled MEG data, the optimized operator yielded close-to-perfect intra-parcel coherence.
    Comparison with existing methods: Previous suggestions for obtaining parcel time series include averaging all source time series within each anatomical parcel or using exclusively the time series of the voxel with maximum power. These methods are sensitive to signal heterogeneity and outlier sources. The approach advanced here avoids these problems.
    Conclusions: The optimized operator is suitable for collapsing real source-reconstructed M/EEG data into any cortical parcellation. The enhanced time series reconstruction fidelity yields improved accuracy of subsequent analyses of both local dynamics and large-scale interaction mapping.
    MeSH term(s) Adult ; Algorithms ; Brain Mapping/methods ; Cerebral Cortex/anatomy & histology ; Cerebral Cortex/physiology ; Computer Simulation ; Electroencephalography/methods ; Female ; Humans ; Linear Models ; Magnetoencephalography/methods ; Male ; Models, Neurological ; ROC Curve ; Signal Processing, Computer-Assisted
    Language English
    Publishing date 2014-02-06
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Validation Studies
    ZDB-ID 282721-9
    ISSN 1872-678X ; 0165-0270
    ISSN (online) 1872-678X
    ISSN 0165-0270
    DOI 10.1016/j.jneumeth.2014.01.031
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  6. Article: Age-related differences in 100-m sprint performance in male and female master runners.

    Korhonen, Marko T / Mero, Antti / Suominen, Harri

    Medicine and science in sports and exercise

    2003  Volume 35, Issue 8, Page(s) 1419–1428

    Abstract: ... characteristics were related to the overall decline in the performance of the 100 m with age.: Methods ... markers at 10-m intervals. Velocity, stride length (SL), stride rate (SR), ground contact time (CT), and ... flight time (FT) during the acceleration, peak velocity, and deceleration phases of the 100-m race were ...

    Abstract Purpose: This study was undertaken to investigate age-related differences in the velocity and selected stride parameters in male and female master sprinters and to determine which stride characteristics were related to the overall decline in the performance of the 100 m with age.
    Methods: The performances of 70 finalists (males 40-88 yr, females 35-87 yr) at the European Veterans Athletics Championships were recorded using two high-speed cameras (200 Hz) with a panning video technique and distance markers at 10-m intervals. Velocity, stride length (SL), stride rate (SR), ground contact time (CT), and flight time (FT) during the acceleration, peak velocity, and deceleration phases of the 100-m race were determined from the video records with the aid of the Peak Performance analysis system.
    Results: There was a general decline in sprint performances with age, the decrease becoming more evident around 65-70 yr of age. The velocity during the different phases of the run declined on average from 5 to 6% per decade in males and from 5 to 7% per decade in females. Similarly, SL showed clear reductions with increasing age, whereas SR remained unchanged until the oldest age groups in both genders. Furthermore, the CT, which correlated with velocity, was significantly longer, and FT, which correlated with both velocity and SL, was shorter in older age groups.
    Conclusion: Our findings indicated that age-associated differences in velocity in elite master sprinters were similar in each phase of the 100-m run. The deterioration of the overall performance with age was primarily related to reduction in SL and increase in CT.
    MeSH term(s) Acceleration ; Adult ; Aged ; Aged, 80 and over ; Aging/physiology ; Biomechanical Phenomena ; Female ; Gait/physiology ; Humans ; Male ; Middle Aged ; Running/physiology ; Sex Factors ; Task Performance and Analysis ; Time Factors ; Video Recording
    Language English
    Publishing date 2003-08
    Publishing country United States
    Document type Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 603994-7
    ISSN 1530-0315 ; 0195-9131 ; 0025-7990
    ISSN (online) 1530-0315
    ISSN 0195-9131 ; 0025-7990
    DOI 10.1249/01.MSS.0000079080.15333.CA
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  7. Article ; Online: Trends in Life Expectancy in Residential Long-Term Care by Sociodemographic Position in 1999-2018: A Multistate Life Table Study of Finnish Older Adults.

    Korhonen, Kaarina / Moustgaard, Heta / Murphy, Michael / Martikainen, Pekka

    The journals of gerontology. Series B, Psychological sciences and social sciences

    2024  

    Abstract: Objectives: Residential long-term care (LTC) use has declined in many countries over the past years. This study quantifies how changing rates of entry, exit, and mortality have contributed to trends in life expectancy in LTC (i.e., average time spent in ...

    Abstract Objectives: Residential long-term care (LTC) use has declined in many countries over the past years. This study quantifies how changing rates of entry, exit, and mortality have contributed to trends in life expectancy in LTC (i.e., average time spent in LTC after age 65) across sociodemographic groups.
    Methods: We analyzed population-register data of all Finns aged ≥65 during 1999-2018 (n=2,016,987) with dates of LTC and death, and sociodemographic characteristics. We estimated transition rates between home, LTC and death using Poisson generalized additive models, and calculated multistate life tables across 1999-2003, 2004-2008, 2009-2013 and 2014-2018.
    Results: Between 1999-2003 and 2004-2008, life expectancy in LTC increased from 0.75 (95% CI 0.74-0.76) to 0.89 (0.88-0.90) years among men and from 1.61 (1.59-1.62) to 1.83 (1.81-1.85) years among women, mainly due to declining exit rates from LTC. Thereafter, life expectancy in LTC decreased, reaching 0.80 (0.79-0.81) and 1.51 (1.50-1.53) years among men and women, respectively, in 2014-2018. Especially among women and non-married men, the decline was largely due to increasing death rates in LTC. Admission rates declined throughout the study period, which offset the increase in life expectancy in LTC attributable to declining mortality in the community. Marital status differences in life expectancy in LTC narrowed over time.
    Discussion: Recent declines in LTC use were driven by postponed LTC admission closer to death. The results suggest that across sociodemographic strata older adults enter LTC in ever worse health and spent a shorter time in care than before.
    Language English
    Publishing date 2024-04-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1223664-0
    ISSN 1758-5368 ; 1079-5014
    ISSN (online) 1758-5368
    ISSN 1079-5014
    DOI 10.1093/geronb/gbae067
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  8. Article: Organization of genes expressing the blood-group-M-specific hemagglutinin of Escherichia coli: identification and nucleotide sequence of the M-agglutinin subunit gene.

    Rhen, M / Väisänen-Rhen, V / Saraste, M / Korhonen, T K

    Gene

    1986  Volume 49, Issue 3, Page(s) 351–360

    Abstract: The organization of genes encoding the blood group M-specific hemagglutinin (M-agglutinin ... identified as the M-agglutinin subunit by its reactivity with anti-M-agglutinin serum. Nucleotide sequence ... analysis of the corresponding gene showed that the M-agglutinin precursor had a 24-amino acid (aa ...

    Abstract The organization of genes encoding the blood group M-specific hemagglutinin (M-agglutinin) of Escherichia coli strain IH11165 was studied with a cloned 6.5-kb DNA segment. This DNA segment contains at least five genes which code for the polypeptides of 12.5, 30, 80, 18.5 and 21 kDa. The 30-, 80- and 21-kDa polypeptides are synthesized as precursors that are approximately 2 kDa larger. The 21-kDa polypeptide was identified as the M-agglutinin subunit by its reactivity with anti-M-agglutinin serum. Nucleotide sequence analysis of the corresponding gene showed that the M-agglutinin precursor had a 24-amino acid (aa) signal sequence, while the mature protein is 146 aa residues long. Although the organization of the M-agglutinin gene cluster resembles those of other E. coli adhesins, there is no significant sequence homology between the M-agglutinin subunit and the subunits of the other potentially related proteins in E. coli.
    MeSH term(s) Amino Acid Sequence ; Base Sequence ; Chromosome Mapping ; Cloning, Molecular ; DNA Restriction Enzymes ; DNA, Bacterial/genetics ; Escherichia coli/genetics ; Fimbriae, Bacterial/physiology ; Genes, Bacterial ; Hemagglutinins/genetics ; Humans ; MNSs Blood-Group System ; Protein Conformation ; Solubility
    Chemical Substances DNA, Bacterial ; Hemagglutinins ; MNSs Blood-Group System ; DNA Restriction Enzymes (EC 3.1.21.-)
    Language English
    Publishing date 1986
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 391792-7
    ISSN 1879-0038 ; 0378-1119
    ISSN (online) 1879-0038
    ISSN 0378-1119
    DOI 10.1016/0378-1119(86)90371-9
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  9. Article ; Online: No ageing-related increase in fibre type grouping in sprint-trained masters runners: A 10-year follow-up study.

    Messa, Guy Anselme Mpaka / Korhonen, Marko T / Degens, Hans

    Journal of cachexia, sarcopenia and muscle

    2024  Volume 15, Issue 2, Page(s) 552–561

    Abstract: ... in the 60-m sprint (P < 0.001) without significant changes in fibre type composition and fibre cross ...

    Abstract Background: Previous research suggests that an ageing-associated remodelling and loss of motor units due to motor neuron death contributes significantly to muscle weakness in old age. In histological sections, motor unit remodelling is reflected by increased fibre type grouping. While regular exercise may not attenuate the loss of motor units during ageing, it has been suggested to facilitate reinnervation resulting in larger motor units, and a higher number and larger fibre type groups in histological sections of muscles from aged individuals.
    Methods: In a 10-year follow-up study, we assessed changes in the prevalence and size of fibre type groups in the vastus lateralis muscle from 34 male masters sprinters (40-85 years at start).
    Results: Over the 10 years, there was an ageing-related reduction in performance in the 60-m sprint (P < 0.001) without significant changes in fibre type composition and fibre cross-sectional area. Neither the number of fibre type groups, defined as a fibre surrounded exclusively by fibres of the same type, nor the group size changed significantly in the 10-year period.
    Conclusions: These histological data show that there is limited to no significant fibre type grouping over a 10-year period in masters athletes who continued sprint run training. This observation challenges the paradigm that ageing, at least in systematically trained sprinters, is associated with motor unit remodelling.
    MeSH term(s) Aged ; Humans ; Male ; Aging/physiology ; Exercise ; Follow-Up Studies ; Motor Neurons ; Quadriceps Muscle ; Adult ; Middle Aged ; Aged, 80 and over
    Language English
    Publishing date 2024-01-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2586864-0
    ISSN 2190-6009 ; 2190-5991
    ISSN (online) 2190-6009
    ISSN 2190-5991
    DOI 10.1002/jcsm.13416
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  10. Article: A Multiprofessional and Intersectoral Working Model to Detect and Support Preschool Children With Neurodevelopmental Difficulties (PLUSS Model): Protocol for an Evaluation Study.

    Gustafsson, Berit M / Korhonen, Laura

    JMIR research protocols

    2022  Volume 11, Issue 6, Page(s) e34969

    Abstract: Background: Neurodevelopmental difficulties with various emotional and behavioral symptoms increase the risk of mental health problems later in life. Although we know that early detection and interventions are effective, there is a lack of intersectoral, ...

    Abstract Background: Neurodevelopmental difficulties with various emotional and behavioral symptoms increase the risk of mental health problems later in life. Although we know that early detection and interventions are effective, there is a lack of intersectoral, integrative, and evidence-based working models to provide these services for preschool children and their parents. PLUSS (Psykisk hälsa Lärande Utveckling Samverkan kring Små barn; English translation: mental health, learning, development, collaboration around preschool children) is a collaborative "one way in" model involving parents, health care providers, preschools, social services, and researchers. PLUSS provides coordinated services to screen, evaluate, and support toddlers with neurodevelopmental problems. It also offers parental interventions and education for preschool teachers.
    Objective: The model will be studied in a research project that aims to investigate (1) using a quasi-experimental study on longitudinal trajectories of neurodevelopmental difficulties and ability to function among participating preschoolers, (2) user satisfaction, and (3) implementation of the model and its effectiveness. The long-term goal is to provide evidence-based, coordinated services to reduce problems related to neurodevelopmental difficulties among preschool children and promote well-being and functioning in everyday life.
    Methods: The population of interest is children aged 1.5-5 years, whom the child health care nurse refers for further assessment due to suspected neurodevelopmental problems. Data are collected using questionnaires and semistructured interviews. Measures include sociodemographic data, longitudinal data on neurodevelopmental problems, parental well-being and satisfaction, the effectiveness of parental and preschool teacher training and implementation of the model, and fostered multisectoral collaborations. Data will be analyzed with qualitative and quantitative methods.
    Results: The PLUSS model has been approved by the National Ethics Review Board (2019-04839). This study was supported by FUTURUM grants 910161 and 910441. Data collection started in April 2019, with the data collection period planned to end in May 2024.
    Conclusions: PLUSS is an integrative working model with multiprofessional competence and intersectoral collaboration capacity to help preschool children with neurodevelopmental problems and their parents. It will be studied using quasi-experimental cross-sectional and longitudinal study designs. Data will be collected from parents, health care providers, and preschool teachers, and will be analyzed using quantitative and qualitative methods. The study will run in one Swedish county, and generalizability needs to be studied separately. Loss of follow-up could impact the longitudinal analysis.
    Trial registration: ClinicalTrials.gov NCT04815889; https://clinicaltrials.gov/ct2/show/NCT04815889.
    International registered report identifier (irrid): DERR1-10.2196/34969.
    Language English
    Publishing date 2022-06-15
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 2719222-2
    ISSN 1929-0748
    ISSN 1929-0748
    DOI 10.2196/34969
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