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Article ; Online: Assessing the metabolism, phylogenomic, and taxonomic classification of the halophilic genus Halarchaeum.

Wang, Shuang / Narsing Rao, Manik Prabhu / Quadri, Syed Raziuddin

2024 Volume 371

Abstract: In this study, a genomic approach was employed to evaluate the metabolic potentials and taxonomic classification of the halophilic genus Halarchaeum. Genomic analysis revealed that Halarchaeum members exhibit a predilection for amino acids as their ... ...

Abstract	In this study, a genomic approach was employed to evaluate the metabolic potentials and taxonomic classification of the halophilic genus Halarchaeum. Genomic analysis revealed that Halarchaeum members exhibit a predilection for amino acids as their primary energy source in high-salinity environments over carbohydrates. Genome analysis unveiled the presence of crucial genes associated with metabolic pathways, including the Embden-Meyerhof pathway, semi-phosphorylative Entner-Doudoroff pathway, and the urea cycle. Furthermore, the genomic analysis indicated that Halarchaeum members employ diverse mechanisms for osmotic regulation (encompassing both salt-in and salt-out strategies). Halarchaeum members also encode genes to alleviate acid and heat stress. The average nucleotide identity value between Halarchaeum solikamskense and Halarchaeum nitratireducens exceeded the established threshold (95%-96%) for defining distinct species. This high similarity suggests a close relationship between these two species, prompting the proposal to reclassify Halarchaeum solikamskense as a heterotypic synonym of Halarchaeum nitratireducens. The results of this study contribute to our knowledge of taxonomic classification and shed light on the adaptive strategies employed by Halarchaeum species in their specific ecological niches.
MeSH term(s)	Phylogeny ; Halobacteriaceae/genetics ; Glycolysis ; Metabolic Networks and Pathways ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; DNA, Bacterial
Chemical Substances	RNA, Ribosomal, 16S ; DNA, Bacterial
Language	English
Publishing date	2024-01-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752343-9
ISSN	1574-6968 ; 0378-1097
ISSN (online)	1574-6968
ISSN	0378-1097
DOI	10.1093/femsle/fnae001
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Article: Canine retraction and anchorage loss using self-ligating and conventional brackets with sliding mechanics: A split-mouth clinical study.

Tiwari, Anurag / Aafaque, Syed / Rizwana, Y / Quadri, Syed Altafuddin / Kanagasabapathy, B / Villuri, Chandrika / Babu, J Suresh / Swarnalatha, C / Nayyar, Abhishek Singh

Journal of orthodontic science

2023 Volume 12, Page(s) 70

Abstract: ... variables studied, while Pearson's correlation coefficient was used to evaluate the correlation between ...

Abstract	Objective: Appliance biocompatibility, orthodontic treatment efficiency and patient convenience are the major issues confronting contemporary orthodontic practice. Very few studies have been published till date regarding the efficiency of self-ligating brackets as against conventional brackets. Hence, the present study was planned to compare the rate of canine retraction between self-ligating and conventional brackets and to determine the amount of anchorage loss during canine retraction. Methods: The present clinical study was designed as a prospective, observational study comprising of 25 patients requiring first premolar extraction as a part of orthodontic treatment. Self-ligating and conventional brackets were bonded using a split-mouth study design randomly. Retraction of canines was done with 150 grams of force using Dontrix gauge with E-chains. The study was conducted in relation to upper arch only, while the rate of retraction was evaluated every 4 weeks for 3 months. Average rates of retraction in 3 months were calculated. For anchorage loss, an acrylic guide plug was used in mid-treatment cast (T0) and after 3 months of retraction (T3). The statistical analysis was done using Statistical Package for Social Sciences (SPSS) version 17.0 (SPSS Inc., Chicago, IL, USA). Independent t-test was used to compare the means of the two variables studied, while Pearson's correlation coefficient was used to evaluate the correlation between the variables studied in the groups included. Results: The correlation coefficient between the average rate of canine retraction with self-ligating brackets vs. conventional brackets over a period of 3 months came out to be 0.6434, while on comparing the data in terms of anchorage loss over a period of 3 months, the respective correlation coefficient value was found to be 0.6659 with the results being statistically highly significant in either case ( Conclusions: Self-ligating brackets showed double the amount of displacement compared to conventional brackets in some of the cases. Also, chair side time was significantly reduced with self-ligating brackets as against conventional brackets.
Language	English
Publishing date	2023-11-02
Publishing country	India
Document type	Journal Article
ZDB-ID	2733594-X
ISSN	2278-0203 ; 2278-1897
ISSN (online)	2278-0203
ISSN	2278-1897
DOI	10.4103/jos.jos_29_23
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Article ; Online: A Thematic Analysis of Online Uveitis Support Groups.

Rasheed, Haroon Adam / Rasheed, Yusuf Salman / Syed-Quadri, Shafee / Tsui, Edmund

Ocular immunology and inflammation

2023 , Page(s) 1–6

Abstract: Purpose: To report the availability and activity of online uveitis support groups.: Methods: An online search was conducted for support groups for uveitis. Member count and activity were recorded. Posts and comments were graded along five themes: ... ...

Abstract	Purpose: To report the availability and activity of online uveitis support groups. Methods: An online search was conducted for support groups for uveitis. Member count and activity were recorded. Posts and comments were graded along five themes: emotional or personal story sharing, information seeking, offer of outside information, emotional support, and expressions of gratitude. Results: An online search resulted in 32 support groups for uveitis. Across all groups, there was a median membership of 725 (IQR 1410.5). Of the 32 groups, five were active and accessible at the time of study. In these five groups, 337 posts and 1406 comments were made within the past year. The most prevalent theme in posts consisted of information seeking (84%) while the most prevalent theme in comments consisted of emotion or personal story sharing (65%). Conclusions: Online uveitis support groups provide a unique space for emotional support, information sharing, and community building.
Language	English
Publishing date	2023-02-21
Publishing country	England
Document type	Journal Article
ZDB-ID	1193873-0
ISSN	1744-5078 ; 0927-3948
ISSN (online)	1744-5078
ISSN	0927-3948
DOI	10.1080/09273948.2023.2178937
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Article ; Online: Comparative analysis of different types of occlusal splints for the management of sleep bruxism: a systematic review.

Ainoosah, Sultan / Farghal, Ahmed E / Alzemei, Marwa Saad / Saini, Ravinder S / Gurumurthy, Vishwanath / Quadri, Syed Altafuddin / Okshah, Abdulmajeed / Mosaddad, Seyed Ali / Heboyan, Artak

BMC oral health

2024 Volume 24, Issue 1, Page(s) 29

Abstract: Background: Sleep bruxism is a prevalent condition in dentistry practice, characterized by involuntary grinding or clenching of the teeth during sleep. Several therapies, including occlusal splints, have been used to manage sleep bruxism and ... ...

Abstract	Background: Sleep bruxism is a prevalent condition in dentistry practice, characterized by involuntary grinding or clenching of the teeth during sleep. Several therapies, including occlusal splints, have been used to manage sleep bruxism and temporomandibular disorders, including occlusal splints. This study aimed to compare the effectiveness of different occlusal splints in managing sleep bruxism. Methods: The PICO framework encompasses the characterization of the population, intervention, comparison, and pertinent outcomes. A comprehensive and systematic literature review was conducted on PubMed, Scopus, and Google Scholar to identify grey literature. The search specifically targeted scientific studies published before September 20, 2023. The Cochrane Collaboration Risk of Bias Tool assessed the accuracy of the included Randomized Control Trials (RCTs). The modified Newcastle-Ottawa Scale assessed non-randomized studies. Data were systematically extracted, synthesized, and reported thematically. Results: Out of the total of 808 articles that were evaluated, only 15 articles were found to meet the specified inclusion criteria. Adjustable splints, such as full-occlusion biofeedback splints, were more effective in reducing sleep bruxism episodes, improving patient-reported symptoms, and enhancing overall well-being. The impact of different occlusal sprints on electromyographic activity varies, and potential adverse effects should be considered individually. Conclusions: This review provides valuable insights into the effectiveness of occlusal splints in managing sleep bruxism. The results of this study indicate that occlusal splint therapy is a viable treatment approach for sleep bruxism.
MeSH term(s)	Humans ; Occlusal Splints ; Sleep Bruxism/therapy ; Sleep
Language	English
Publishing date	2024-01-05
Publishing country	England
Document type	Systematic Review ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2091511-1
ISSN	1472-6831 ; 1472-6831
ISSN (online)	1472-6831
ISSN	1472-6831
DOI	10.1186/s12903-023-03782-6
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Article: Arsenic in the Food Chain in Pakistan: Assessing Risks to Human Health and Ensuring Food Security Through Comprehensive Contamination Mitigation Strategies.

Quadri, Syed Shameel Ahmed / Ali, Rabia / Mesidor, Anderson / Shah, Imam Ali / Raqeeb, Nimra / Omer Afzal, Muhammad / Rizwan, Muhammad / Rafique, Tariq / Nosheen, Saba

Cureus

2024 Volume 16, Issue 2, Page(s) e54069

Abstract: Arsenic is a natural element found in the earth's crust and is extensively present in various ...

Abstract	Arsenic is a natural element found in the earth's crust and is extensively present in various environmental components. Anthropogenic activities and a few natural events have generated contaminants that have led to massive environmental pollution, one form of which is arsenic contamination. Arsenic enters the human food chain via contaminated crops, water, seafood, and dairy products. In Pakistan, the increasing concentration of arsenic in the water is causing major health problems. Due to the serious health risks posed by arsenic, it is crucial to design and implement strategies for reducing and preventing the bioaccumulation of arsenic and its entry into the human food chain. There is a need for an institutional framework for arsenic mitigation, accountability, and systemic checks and balances. Targeted short- and long-term policies are required for effective and sustainable management.
Language	English
Publishing date	2024-02-12
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.54069
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Article ; Online: Single-cell western blotting.

Quadri, Syed M S

Methods in molecular biology (Clifton, N.J.)

2015 Volume 1312, Page(s) 455–464

Abstract: Cell heterogeneity is a variation in cellular processes in functionally similar cells. Cells from the same tissue which are considered genetically identical may have difference in size, structure, and level of protein expression which can lead to major ... ...

Abstract	Cell heterogeneity is a variation in cellular processes in functionally similar cells. Cells from the same tissue which are considered genetically identical may have difference in size, structure, and level of protein expression which can lead to major impact on the functions of cell leading to difference in physiological consequences. Single-cell proteome-wide studies are used to detect cell heterogeneity. Flow cytometry and immunocytochemistry do play an important role in evaluating cell heterogeneity. However, these methods are based on separation by antibodies with limited specificity. Cross-reactivity can occur leading to bias in result. Western blot is done to separate the proteins according to molecular weight. Therefore, off-target and on-target signals can be discriminated. Detection of protein expression from a tissue can be done with the help of western blot. However, it is unable to differentiate protein expression of individual cells. For detection of this cell-to-cell variation, a highly advanced technique termed "single-cell western blotting" is carried out. Single-cell western blot has enabled us to detect protein expression at cellular level at a fairly advanced high resolution using a western blot designed to assess cell heterogeneity.
MeSH term(s)	Animals ; Blotting, Western/methods ; Cell Culture Techniques ; Humans ; Neural Stem Cells/cytology ; Proteins/analysis ; Proteins/isolation & purification ; Rats ; Single-Cell Analysis/methods
Chemical Substances	Proteins
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-2694-7_46
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Article ; Online: Parafilm-M®, An Available Cost-Effective Alternative for Immuno-blot Pouches.

Quadri, Syed M S

Methods in molecular biology (Clifton, N.J.)

2015 Volume 1314, Page(s) 313–323

Abstract: Commercially available standard immuno-blot pouches do play an efficient role in antibody incubation in performing an immuno-blot, but are not readily available in the laboratory and have to be specifically ordered. We have developed an equally efficient ...

Abstract	Commercially available standard immuno-blot pouches do play an efficient role in antibody incubation in performing an immuno-blot, but are not readily available in the laboratory and have to be specifically ordered. We have developed an equally efficient technique to make an immune-blot more cost-effective with more conservation of antibodies by using a common and readily available laboratory product Parafilm-M(®). Parafilm-M(®) which serves as a sealant for various items of laboratory equipment can be used for antibody incubation. Manually made Parafilm-M(®) pouch has a clear advantage over standard immuno-blot pouches in terms of availability, cost-effectiveness, and consumption of antibodies that ultimately reduces the cost of an immuno-blot. We have performed a series of experiments to check the efficacy of both the techniques. Samples with equal amount of protein were analyzed on separate SDS PAGE gels. The proteins were transferred electrophoretically to the nitrocellulose membrane using Trans-Blot(®) Turbo™ Mini Nitrocellulose Transfer Pack. Antibody incubation was done using standard immuno-blot pouch, standard container and Parafilm-M(®) sealed pouch. The expression of protein was determined and the results of immuno-blots were compared. We found that antibodies are binding the membrane in Parafilm-M(®) pouches as efficiently as in container method or in standard immuno-blot pouches. By restricting the membrane, the surface area of the manually made Parafilm-M(®) pouch can be reduced, less diluent is required to cover the membrane as a result less antibodies are consumed. We also calculated that each immuno-blot pouch cost around $0.1906, whereas the cost for Parafilm-M(®) pouch is 0.0695 which is almost one-third the price of an immuno-blot pouch. Thus, Parafilm-M(®) method distinctly provides a cost-effective solution for antibody incubation.
MeSH term(s)	Animals ; Cost-Benefit Analysis ; Electrophoresis, Polyacrylamide Gel/methods ; Humans ; Immunoblotting/economics ; Immunoblotting/methods ; Paraffin/chemistry ; Proteins/analysis
Chemical Substances	Proteins ; Paraffin (8002-74-2)
Language	English
Publishing date	2015
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-4939-2718-0_32
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Article ; Online: The flexural strength of 3D-printed provisional restorations fabricated with different resins: a systematic review and meta-analysis.

Saini, Ravinder S / Gurumurthy, Vishwanath / Quadri, Syed Altafuddin / Bavabeedu, Shashit Shetty / Abdelaziz, Khalid M / Okshah, Abdulmajeed / Alshadidi, Abdulkhaliq Ali F / Yessayan, Lazar / Mosaddad, Seyed Ali / Heboyan, Artak

BMC oral health

2024 Volume 24, Issue 1, Page(s) 66

Abstract: Background: Three-dimensional (3D) printing technology has revolutionized dentistry, particularly in fabricating provisional restorations. This systematic review and meta-analysis aimed to thoroughly evaluate the flexural strength of provisional ... ...

Abstract	Background: Three-dimensional (3D) printing technology has revolutionized dentistry, particularly in fabricating provisional restorations. This systematic review and meta-analysis aimed to thoroughly evaluate the flexural strength of provisional restorations produced using 3D printing while considering the impact of different resin materials. Methods: A systematic search was conducted across major databases (ScienceDirect, PubMed, Web of Sciences, Google Scholar, and Scopus) to identify relevant studies published to date. The inclusion criteria included studies evaluating the flexural strength of 3D-printed provisional restorations using different resins. Data extraction and quality assessment were performed using the CONSORT scale, and a meta-analysis was conducted using RevMan 5.4 to pool results. Results: Of the 1914 initially identified research articles, only 13, published between January 2016 and November 2023, were included after screening. Notably, Digital Light Processing (DLP) has emerged as the predominant 3D printing technique, while stereolithography (SLA), Fused Deposition Modeling (FDM), and mono-liquid crystal displays (LCD) have also been recognized. Various printed resins have been utilized in different techniques, including acrylic, composite resins, and methacrylate oligomer-based materials. Regarding flexural strength, polymerization played a pivotal role for resins used in 3D or conventional/milled resins, revealing significant variations in the study. For instance, SLA-3D and DLP Acrylate photopolymers displayed distinct strengths, along with DLP bisacrylic, milled PMMA, and conventional PMMA. The subsequent meta-analysis indicated a significant difference in flexure strength, with a pooled Mean Difference (MD) of - 1.25 (95% CI - 16.98 - 14.47; P < 0.00001) and a high I Conclusions: This study provides a comprehensive overview of the flexural strength of 3D-printed provisional restorations fabricated using different resins. However, further research is recommended to explore additional factors influencing flexural strength and refine the recommendations for enhancing the performance of 3D-printed provisional restorations in clinical applications.
MeSH term(s)	Humans ; Flexural Strength ; Polymethyl Methacrylate ; Composite Resins ; Acrylic Resins ; Printing, Three-Dimensional
Chemical Substances	Polymethyl Methacrylate (9011-14-7) ; Composite Resins ; Acrylic Resins
Language	English
Publishing date	2024-01-10
Publishing country	England
Document type	Meta-Analysis ; Systematic Review ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2091511-1
ISSN	1472-6831 ; 1472-6831
ISSN (online)	1472-6831
ISSN	1472-6831
DOI	10.1186/s12903-023-03826-x
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Article ; Online: The effectiveness of botulinum toxin for temporomandibular disorders: A systematic review and meta-analysis.

Saini, Ravinder S / Ali Abdullah Almoyad, Muhammad / Binduhayyim, Rayan Ibrahim H / Quadri, Syed Altafuddin / Gurumurthy, Vishwanath / Bavabeedu, Shashit Shetty / Kuruniyan, Mohammed Saheer / Naseef, Punnoth Poonkuzhi / Mosaddad, Seyed Ali / Heboyan, Artak

PloS one

2024 Volume 19, Issue 3, Page(s) e0300157

Abstract: Objective: The current body of research on utilizing botulinum toxin (BTX) to manage temporomandibular disorders (TMDs) has not yet yielded definitive conclusions. The primary objective of this study was to determine the effectiveness of BTX in pain ... ...

Abstract	Objective: The current body of research on utilizing botulinum toxin (BTX) to manage temporomandibular disorders (TMDs) has not yet yielded definitive conclusions. The primary objective of this study was to determine the effectiveness of BTX in pain reduction for TMDs compared to placebo and other treatments. The secondary outcomes evaluated were adverse events, maximum mouth opening, bruxism events, and maximum occlusal force. Materials and methods: A literature search was performed on PubMed, Dimension Publication, Scopus, and Google Scholar. The RoB 2 tool was used for quality assessment. The mean differences in pain scores were estimated to measure the effect of BTX on pain reduction. For adverse events, the risk ratio for the incidence of side effects was calculated. Results: Two hundred and sixty non-duplicate articles were identified; however, only 14 RCTS were included in this review. The total study population included 395 patients. The overall risk of bias showed a low to moderate quality of evidence. Results from 6 studies were reported only narratively; four studies were used for meta-analysis on pain reduction, and five were used for meta-analysis on adverse events. The control used in the meta-analysis was placebo injections. Results of the meta-analysis for pain reduction were statistically insignificant for the BTX group with mean differences at MD = -1.71 (95% CI, -2.87 to -0.5) at one month, -1.53 (95% CI, -2.80 to -0.27) at three months, and -1.33 (95% CI, -2.74 to 0.77) at six months. This showed that BTX treatment was not significantly better than placebo for a reduction in pain scores at 1, 3, and 6 months. Regarding safety, the placebo group showed a relative risk of 1.34 (95%CI, 0.48-6.78) and 1.17 (95%CI, 0.54-3.88) at 1 and 3 months respectively. However, the risks were not statistically significant. There was also no difference in the effectiveness of BTX compared to placebo and other treatments for maximum mouth opening, bruxism events, and maximum occlusal force. Conclusion: BTX was not associated with better outcomes in terms of pain reduction, adverse events, maximum mouth opening, bruxism events, and maximum occlusal force. More high-quality RCTs are needed to better understand this topic.
MeSH term(s)	Humans ; Botulinum Toxins, Type A/adverse effects ; Bruxism/drug therapy ; Pain/drug therapy ; Temporomandibular Joint Disorders/drug therapy ; Bite Force
Chemical Substances	Botulinum Toxins, Type A (EC 3.4.24.69)
Language	English
Publishing date	2024-03-14
Publishing country	United States
Document type	Meta-Analysis ; Systematic Review ; Journal Article
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0300157
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Article: The Pathological Role of Pro(Renin) Receptor in Renal Inflammation.

Quadri, Syed S / Cooper, Caleb / Ghaffar, Dawood / Vaishnav, Hitesh / Nahar, Ludmila

Journal of experimental pharmacology

2021 Volume 13, Page(s) 339–344

Abstract: Pro)renin receptor (PRR) is the recently discovered component of the renin-angiotensin-aldosterone system (RAS). Many organs contain their own RAS, wherein PRR can exert organ-specific localized effects. The Binding of prorenin/renin to PRR activates ... ...

Abstract	(Pro)renin receptor (PRR) is the recently discovered component of the renin-angiotensin-aldosterone system (RAS). Many organs contain their own RAS, wherein PRR can exert organ-specific localized effects. The Binding of prorenin/renin to PRR activates angiotensin-dependent and independent pathways which leads to the development of physiological and pathological effects. Continued progress in PRR research suggests that the upregulation of PRR contributes to the development of hypertension, glomerular injury, and progression of kidney disease and inflammation. In the current review, we highlight the function of the PRR in renal inflammation in pathophysiological conditions.
Language	English
Publishing date	2021-03-19
Publishing country	New Zealand
Document type	Journal Article ; Review
ZDB-ID	2587465-2
ISSN	1179-1454
ISSN	1179-1454
DOI	10.2147/JEP.S297682
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