Article: Transcriptomic analysis and carotenogenic gene expression related to petal coloration in Osmanthus fragrans âYanhong Guiâ
Trees. 2016 Aug., v. 30, no. 4
2016
Abstract: ... in Osmanthus fragrans âYanhong Guiâ. We identified 15 key carotenogenic genes involved in petal coloration ... of O. fragrans âYanhong Guiâ. Osmanthus fragrans Lour. is a famous traditional woody flower ... cultivar âYanhong Guiâ of O. fragrans. Results showed that α-carotene and β-carotene were identified ...
| Abstract | KEY MESSAGE : α-Carotene and β-carotene were the major substances of petal coloration in Osmanthus fragrans âYanhong Guiâ. We identified 15 key carotenogenic genes involved in petal coloration of O. fragrans âYanhong Guiâ. Osmanthus fragrans Lour. is a famous traditional woody flower in China. Carotenoids are the major coloring factor in the petals of O. fragrans. However, little information is available on the transcriptional regulation of carotenogenic genes on petal coloration of orange-red-flowered cultivar. In this study, petal color, carotenoid content, transcriptome dynamics of flower buds and expression of key carotenogenic genes were analyzed by use of an orange-red-flowered cultivar âYanhong Guiâ of O. fragrans. Results showed that α-carotene and β-carotene were identified as the major substances of petal coloration in O. fragrans âYanhong Guiâ. Using transcriptome sequencing technology, about 54.79, 57.79, 54.71 and 58.04 million raw reads were obtained and assembled into 175,320, 177,162, 175,248 and 161,902 unigenes from four cDNA libraries, respectively. Out of these assembled unigenes, a total of 129,670, 65,655 and 49,837 unigenes were assigned to non-redundant protein, Gene Ontology and Cluster of Orthologous Groups of proteins database, respectively. Furthermore, out of 46 unigenes which were identified to be carotenoid-related genes, 35 differentially expressed genes (DEGs) were generated with the threshold of fold change â¥2 or â¤0.5. Based on the expression levels, 35 DEGs were grouped into five clusters using the hierarchical method. Taken petal color development and carotenoid content into consideration, we identified 27 candidate key DEGs involved in carotenoid metabolism during flower bud development. Further analysis with quantitative real-time PCR generated 15 key carotenogenic genes involved in petal coloration of O. fragrans âYanhong Guiâ. In addition to providing valuable sequence resource of O. fragrans, all the results and analysis are valuable resources for better understanding of carotenoids metabolism in this plant. |
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| Keywords | alpha-carotene ; beta-carotene ; buds ; cDNA libraries ; color ; complementary DNA ; corolla ; cultivars ; databases ; gene expression ; gene expression regulation ; metabolism ; Osmanthus fragrans ; proteins ; quantitative analysis ; quantitative polymerase chain reaction ; transcription (genetics) ; transcriptome ; transcriptomics ; unigenes ; China |
| Language | English |
| Dates of publication | 2016-08 |
| Size | p. 1207-1223. |
| Publishing place | Springer Berlin Heidelberg |
| Document type | Article |
| ZDB-ID | 90595-1 |
| ISSN | 1432-2285 ; 0931-1890 |
| ISSN (online) | 1432-2285 |
| ISSN | 0931-1890 |
| DOI | 10.1007/s00468-016-1359-8 |
| Database | NAL-Catalogue (AGRICOLA) |
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