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  1. Article ; Online: Influences: Short circuits.

    Clapham, David E

    The Journal of general physiology

    2018  Volume 150, Issue 4, Page(s) 513–515

    MeSH term(s) Electrophysiology/history ; History, 20th Century ; History, 21st Century ; Membrane Potentials ; United States
    Language English
    Publishing date 2018-03-20
    Publishing country United States
    Document type Autobiography ; Biography ; Historical Article ; Journal Article ; Portrait
    ZDB-ID 3118-5
    ISSN 1540-7748 ; 0022-1295
    ISSN (online) 1540-7748
    ISSN 0022-1295
    DOI 10.1085/jgp.201812034
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    In stock of ZB MED Cologne/Königswinter

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  2. Article ; Online: Structural biology: Pain-sensing TRPA1 channel resolved.

    Clapham, David E

    Nature

    2015  Volume 520, Issue 7548, Page(s) 439–441

    MeSH term(s) Calcium Channels/chemistry ; Calcium Channels/ultrastructure ; Cryoelectron Microscopy ; Humans ; Nerve Tissue Proteins/chemistry ; Nerve Tissue Proteins/ultrastructure ; TRPA1 Cation Channel ; Transient Receptor Potential Channels/chemistry ; Transient Receptor Potential Channels/ultrastructure
    Chemical Substances Calcium Channels ; Nerve Tissue Proteins ; TRPA1 Cation Channel ; TRPA1 protein, human ; Transient Receptor Potential Channels
    Language English
    Publishing date 2015-04-23
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/nature14383
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    Zs.A 26: Show issues Location:
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    Zs.MG 9: Show issues
    Zs.MO 244: Show issues

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  3. Book ; Conference proceedings: Organellar ion channels and transporters

    Clapham, David E.

    Marine Biological Laboratory, Woods Hole, Massachusetts, 7 - 9 September 1995

    (... annual symposium / Society of General Physiologists ; 49 ; Society of General Physiologists series ; 51)

    1996  

    Event/congress International Symposium on Organellar Ion Channels and Transporters (1995, WoodsHoleMass.)
    Author's details [International Symposium on Organellar Ion Channels and Transporters]. Ed. by David E. Clapham
    Series title ... annual symposium / Society of General Physiologists ; 49
    Society of General Physiologists series ; 51
    Annual symposium / Society of General Physiologists
    Collection Annual symposium / Society of General Physiologists
    Keywords Ion Channels / congresses ; Ion Pumps / congresses ; Biological Transport / congresses ; Organell ; Ionenkanal ; Ionentransport
    Subject Ionentranslokation ; Ionenstrom ; Zellorganell
    Language English
    Size X, 275 S. : Ill., graph. Darst.
    Publisher Rockefeller Univ. Press
    Publishing place New York
    Publishing country United States
    Document type Book ; Conference proceedings
    HBZ-ID HT007216913
    ISBN 0-87470-058-2 ; 978-0-87470-058-9
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    1996 A 3363 order for loan Magazin

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  4. Article: Sperm BerserKers.

    Clapham, David E

    eLife

    2013  Volume 2, Page(s) e01469

    Abstract: Human sperm cells rely on an unusual type of potassium ion channel. ...

    Abstract Human sperm cells rely on an unusual type of potassium ion channel.
    MeSH term(s) Humans ; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits/metabolism ; Male ; Spermatozoa/metabolism
    Chemical Substances Large-Conductance Calcium-Activated Potassium Channel alpha Subunits
    Language English
    Publishing date 2013-10-08
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2687154-3
    ISSN 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.01469
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  5. Article: The mother of all endocytosis.

    Clapham, David E

    eLife

    2013  Volume 2, Page(s) e01738

    Abstract: Massive endocytosis is initiated by a series of steps that involve a sudden influx of calcium ions, changes in mitochondria, and modification of surface proteins by lipids. A better understanding of this process could lead to new approaches to reducing ... ...

    Abstract Massive endocytosis is initiated by a series of steps that involve a sudden influx of calcium ions, changes in mitochondria, and modification of surface proteins by lipids. A better understanding of this process could lead to new approaches to reducing the tissue damage that is caused by heart attacks.
    MeSH term(s) Animals ; Endocytosis ; Mitochondria/metabolism ; Palmitic Acid/metabolism
    Chemical Substances Palmitic Acid (2V16EO95H1)
    Language English
    Publishing date 2013-11-26
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 2687154-3
    ISSN 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.01738
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  6. Article ; Online: Visualizing synaptic dopamine efflux with a 2D composite nanofilm.

    Bulumulla, Chandima / Krasley, Andrew T / Cristofori-Armstrong, Ben / Valinsky, William C / Walpita, Deepika / Ackerman, David / Clapham, David E / Beyene, Abraham G

    eLife

    2022  Volume 11

    Abstract: Chemical neurotransmission constitutes one of the fundamental modalities of communication between neurons. Monitoring release of these chemicals has traditionally been difficult to carry out at spatial and temporal scales relevant to neuron function. To ... ...

    Abstract Chemical neurotransmission constitutes one of the fundamental modalities of communication between neurons. Monitoring release of these chemicals has traditionally been difficult to carry out at spatial and temporal scales relevant to neuron function. To understand chemical neurotransmission more fully, we need to improve the spatial and temporal resolutions of measurements for neurotransmitter release. To address this, we engineered a chemi-sensitive, two-dimensional composite nanofilm that facilitates visualization of the release and diffusion of the neurochemical dopamine with synaptic resolution, quantal sensitivity, and simultaneously from hundreds of release sites. Using this technology, we were able to monitor the spatiotemporal dynamics of dopamine release in dendritic processes, a poorly understood phenomenon. We found that dopamine release is broadcast from a subset of dendritic processes as hotspots that have a mean spatial spread of ≈ 3.2 µm (full width at half maximum [FWHM]) and are observed with a mean spatial frequency of one hotspot per ≈ 7.5 µm of dendritic length. Major dendrites of dopamine neurons and fine dendritic processes, as well as dendritic arbors and dendrites with no apparent varicose morphology participated in dopamine release. Remarkably, these release hotspots co-localized with Bassoon, suggesting that Bassoon may contribute to organizing active zones in dendrites, similar to its role in axon terminals.
    MeSH term(s) Dopamine ; Dopaminergic Neurons ; Presynaptic Terminals ; Synaptic Transmission/physiology
    Chemical Substances Dopamine (VTD58H1Z2X)
    Language English
    Publishing date 2022-07-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.78773
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  7. Article ; Online: Recording Electrical Currents across the Plasma Membrane of Mammalian Sperm Cells.

    Liu, Boheng / Mundt, Nadine / Miller, Melissa / Clapham, David E / Kirichok, Yuriy / Lishko, Polina V

    Journal of visualized experiments : JoVE

    2021  , Issue 168

    Abstract: Recording of the electrical activity from one of the smallest cells of a mammalian organism- a sperm cell- has been a challenging task for electrophysiologists for many decades. The method known as "spermatozoan patch clamp" was introduced in 2006. It ... ...

    Abstract Recording of the electrical activity from one of the smallest cells of a mammalian organism- a sperm cell- has been a challenging task for electrophysiologists for many decades. The method known as "spermatozoan patch clamp" was introduced in 2006. It has enabled the direct recording of ion channel activity in whole-cell and cell-attached configurations and has been instrumental in describing sperm cell physiology and the molecular identity of various calcium, potassium, sodium, chloride, and proton ion channels. However, recording from single spermatozoa requires advanced skills and training in electrophysiology. This detailed protocol summarizes the step-by-step procedure and highlights several 'tricks-of-the-trade' in order to make it available to anyone who wishes to explore the fascinating physiology of the sperm cell. Specifically, the protocol describes recording from human and murine sperm cells but can be adapted to essentially any mammalian sperm cell of any species. The protocol covers important details of the application of this technique, such as isolation of sperm cells, selection of reagents and equipment, immobilization of the highly motile cells, formation of the tight (Gigaohm) seal between a recording electrode and the plasma membrane of the sperm cells, transition into the whole-spermatozoan mode (also known as break-in), and exemplary recordings of the sperm cell calcium ion channel, CatSper, from six mammalian species. The advantages and limitations of the sperm patch clamp method, as well as the most critical steps, are discussed.
    MeSH term(s) Animals ; Calcium/metabolism ; Cell Membrane/drug effects ; Cell Membrane/physiology ; Cell Size ; Dissection ; Electrophysiological Phenomena/drug effects ; Flagella/drug effects ; Flagella/physiology ; Humans ; Hydrogen-Ion Concentration ; Ion Transport/drug effects ; Macaca mulatta ; Male ; Mice, Inbred C57BL ; Patch-Clamp Techniques ; Perfusion ; Progesterone/pharmacology ; Solutions ; Spermatozoa/cytology ; Spermatozoa/drug effects ; Spermatozoa/physiology ; Mice
    Chemical Substances Solutions ; Progesterone (4G7DS2Q64Y) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-02-14
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62049
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  8. Article ; Online: A STIMulus Package puts orai calcium channels to work.

    Clapham, David E

    Cell

    2009  Volume 136, Issue 5, Page(s) 814–816

    Abstract: Activation of plasma membrane calcium channels by depletion of endoplasmic reticulum (ER) calcium stores is important for calcium entry in many cell types. Park et al. (2009) now show that direct binding of the ER protein STIM to tetramers of the Orai1 ... ...

    Abstract Activation of plasma membrane calcium channels by depletion of endoplasmic reticulum (ER) calcium stores is important for calcium entry in many cell types. Park et al. (2009) now show that direct binding of the ER protein STIM to tetramers of the Orai1 calcium channel in the plasma membrane triggers opening of this channel.
    MeSH term(s) Animals ; Calcium Channels/metabolism ; Calcium Signaling ; Cell Membrane/metabolism ; Endoplasmic Reticulum/metabolism ; Humans ; Membrane Proteins/metabolism ; Neoplasm Proteins/metabolism ; Stromal Interaction Molecule 1
    Chemical Substances Calcium Channels ; Membrane Proteins ; Neoplasm Proteins ; STIM1 protein, human ; Stromal Interaction Molecule 1
    Language English
    Publishing date 2009-03-06
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2009.02.022
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  9. Book ; Thesis: A whole tissue model of heart cell aggregates

    Clapham, David E.

    electr. coupling between cells, membrane impedance, and the extracellular space

    1979  

    Size XV, 294 Bl. : graph. Darst.
    Publishing country United States
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Atlanta, Ga., Emory Univ., Diss., 1979
    Old title Kopie, erschienen im Verl. Univ. Microfilms Internat., Ann Arbor, Mich., 1988
    HBZ-ID HT003119602
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    88 D 1201 order for loan Magazin

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  10. Article ; Online: Sperm BerserKers

    David E Clapham

    eLife, Vol

    2013  Volume 2

    Abstract: Human sperm cells rely on an unusual type of potassium ion channel. ...

    Abstract Human sperm cells rely on an unusual type of potassium ion channel.
    Keywords Human KSper ; Slo1 ; spermatozoa ; sperm ion channel ; Big Potassium channel ; CatSper ; Medicine ; R ; Science ; Q ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2013-10-01T00:00:00Z
    Publisher eLife Sciences Publications Ltd
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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