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  1. Article ; Online: Developing technologies to unlock the therapeutic and research potential of human stem cells.

    Minger, Stephen L

    New biotechnology

    2013  Volume 30, Issue 4, Page(s) 378–380

    Abstract: Since human embryonic stem cells (hESCs) were first isolated and cultured nearly 15 years ago, stem cell biology has been a promising and fast-moving area of research. Improved clinical predictivity in drug development, use in assays to personalise ... ...

    Abstract Since human embryonic stem cells (hESCs) were first isolated and cultured nearly 15 years ago, stem cell biology has been a promising and fast-moving area of research. Improved clinical predictivity in drug development, use in assays to personalise medicine effectively and as the foundation for cell-based therapies are all areas where stem cells can play an important role. But with opportunities come challenges and it is vital that the field of stem cells continues to progress to achieve its potential. This article outlines the measures the Cell Technologies group at GE Healthcare Life Sciences are taking, along with its collaborators in academia, industry and the clinic, to advance stem cell tools and technologies, as well as identifying some future challenges for stem cell research, drug discovery, cell therapy and regenerative medicine.
    MeSH term(s) Biotechnology ; Humans ; Stem Cell Research ; Stem Cell Transplantation ; Stem Cells
    Language English
    Publishing date 2013-05-25
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 2400836-9
    ISSN 1876-4347 ; 1871-6784
    ISSN (online) 1876-4347
    ISSN 1871-6784
    DOI 10.1016/j.nbt.2012.11.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Interspecies SCNT-derived human embryos--a new way forward for regenerative medicine.

    Minger, Stephen

    Regenerative medicine

    2007  Volume 2, Issue 2, Page(s) 103–106

    MeSH term(s) Animals ; Blastocyst/cytology ; Cell Line ; Disease Models, Animal ; Embryo Culture Techniques ; Embryonic Stem Cells/cytology ; Female ; Fertilization in Vitro ; Genetic Diseases, Inborn/therapy ; Humans ; Nuclear Transfer Techniques ; Oocytes/cytology ; Regenerative Medicine/economics ; Regenerative Medicine/ethics ; Regenerative Medicine/legislation & jurisprudence ; United Kingdom
    Language English
    Publishing date 2007-03
    Publishing country England
    Document type Editorial
    ZDB-ID 2274500-2
    ISSN 1746-076X ; 1746-0751
    ISSN (online) 1746-076X
    ISSN 1746-0751
    DOI 10.2217/17460751.2.2.103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6627: Show issues Location:
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  3. Article: Mesenchymal Stromal Cells: Potential Option for COVID-19 Treatment.

    Primorac, Dragan / Čemerin, Martin / Matišić, Vid / Molnar, Vilim / Strbad, Marko / Girandon, Lenart / Zenić, Lucija / Knežević, Miomir / Minger, Stephen / Polančec, Denis

    Pharmaceutics

    2021  Volume 13, Issue 9

    Abstract: The COVID-19 pandemic has significantly impacted the way of life worldwide and continues to bring high mortality rates to at-risk groups. Patients who develop severe COVID-19 pneumonia, often complicated with ARDS, are left with limited treatment options ...

    Abstract The COVID-19 pandemic has significantly impacted the way of life worldwide and continues to bring high mortality rates to at-risk groups. Patients who develop severe COVID-19 pneumonia, often complicated with ARDS, are left with limited treatment options with no targeted therapy currently available. One of the features of COVID-19 is an overaggressive immune reaction that leads to multiorgan failure. Mesenchymal stromal cell (MSC) treatment has been in development for various clinical indications for over a decade, with a safe side effect profile and promising results in preclinical and clinical trials. Therefore, the use of MSCs in COVID-19-induced respiratory failure and ARDS was a logical step in order to find a potential treatment option for the most severe patients. In this review, the main characteristics of MSCs, their proposed mechanism of action in COVID-19 treatment and the effect of this therapy in published case reports and clinical trials are discussed.
    Language English
    Publishing date 2021-09-16
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527217-2
    ISSN 1999-4923
    ISSN 1999-4923
    DOI 10.3390/pharmaceutics13091481
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  4. Article ; Online: DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.

    Czysz, Katherine / Minger, Stephen / Thomas, Nick

    PloS one

    2015  Volume 10, Issue 2, Page(s) e0117689

    Abstract: Background: Definitive endoderm (DE) is one of the three germ layers which during in vivo vertebrate development gives rise to a variety of organs including liver, lungs, thyroid and pancreas; consequently efficient in vitro initiation of stem cell ... ...

    Abstract Background: Definitive endoderm (DE) is one of the three germ layers which during in vivo vertebrate development gives rise to a variety of organs including liver, lungs, thyroid and pancreas; consequently efficient in vitro initiation of stem cell differentiation to DE cells is a prerequisite for successful cellular specification to subsequent DE-derived cell types [1, 2]. In this study we present a novel approach to rapidly and efficiently down regulate pluripotency genes during initiation of differentiation to DE cells by addition of dimethyl sulfoxide (DMSO) to Activin A-based culture medium and report its effects on the downstream differentiation to hepatocyte-like cells.
    Materials and methods: Human embryonic stem cells (hESC) were differentiated to DE using standard methods in medium supplemented with 100ng/ml of Activin A and compared to cultures where DE specification was additionally enhanced with different concentrations of DMSO. DE cells were subsequently primed to generate hepatic-like cells to investigate whether the addition of DMSO during formation of DE improved subsequent expression of hepatic markers. A combination of flow cytometry, real-time quantitative reverse PCR and immunofluorescence was applied throughout the differentiation process to monitor expression of pluripotency (POUF5/OCT4 & NANOG), definitive endoderm (SOX17, CXCR4 & GATA4) and hepatic (AFP & ALB) genes to generate differentiation stage-specific signatures.
    Results: Addition of DMSO to the Activin A-based medium during DE specification resulted in rapid down regulation of the pluripotency genes OCT4 and NANOG, accompanied by an increase expression of the DE genes SOX17, CXCR4 and GATA4. Importantly, the expression level of ALB in DMSO-treated cells was also higher than in cells which were differentiated to the DE stage via standard Activin A treatment.
    MeSH term(s) Antigens, Differentiation/biosynthesis ; Cell Differentiation/drug effects ; Cryoprotective Agents/pharmacology ; Dimethyl Sulfoxide/pharmacology ; Down-Regulation/drug effects ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism ; Hepatocytes/cytology ; Hepatocytes/metabolism ; Humans ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism
    Chemical Substances Antigens, Differentiation ; Cryoprotective Agents ; Dimethyl Sulfoxide (YOW8V9698H)
    Language English
    Publishing date 2015
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0117689
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  5. Article: Generation of hepatocytes from human embryonic stem cells.

    Safinia, Niloufar / Minger, Stephen L

    Methods in molecular biology (Clifton, N.J.)

    2009  Volume 481, Page(s) 169–180

    Abstract: Use of human hepatocytes for therapeutic and drug discovery applications is hampered by limited tissue source and the inability of hepatocytes to proliferate and maintain function long-term in vitro. Human embryonic stem (hES) cells are immortal and ... ...

    Abstract Use of human hepatocytes for therapeutic and drug discovery applications is hampered by limited tissue source and the inability of hepatocytes to proliferate and maintain function long-term in vitro. Human embryonic stem (hES) cells are immortal and pluripotent and may provide a cell source for functional human hepatocytes (1) Here we have outlined some of the protocols currently in use for the generation of hepatocytes from hES cells.
    MeSH term(s) Cell Culture Techniques/methods ; Cell Differentiation/genetics ; Cell Differentiation/physiology ; Embryonic Stem Cells/metabolism ; Embryonic Stem Cells/physiology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Hepatocytes/metabolism ; Hepatocytes/physiology ; Humans ; Models, Biological ; Reverse Transcriptase Polymerase Chain Reaction/methods
    Language English
    Publishing date 2009
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1007/978-1-59745-201-4_14
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: iPS cells and the politics of promise.

    Gottweis, Herbert / Minger, Stephen

    Nature biotechnology

    2008  Volume 26, Issue 3, Page(s) 271–272

    MeSH term(s) Cell Culture Techniques ; Cell Differentiation ; Embryo Research/ethics ; Embryo Research/legislation & jurisprudence ; Embryonic Stem Cells/cytology ; Humans ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/drug effects ; Politics
    Language English
    Publishing date 2008-03
    Publishing country United States
    Document type Comment ; Letter
    ZDB-ID 1311932-1
    ISSN 1546-1696 ; 1087-0156
    ISSN (online) 1546-1696
    ISSN 1087-0156
    DOI 10.1038/nbt0308-271
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2167: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 137: Show issues
    Zs.MG 43: Show issues

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  7. Article ; Online: DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.

    Katherine Czysz / Stephen Minger / Nick Thomas

    PLoS ONE, Vol 10, Iss 2, p e

    2015  Volume 0117689

    Abstract: BACKGROUND: Definitive endoderm (DE) is one of the three germ layers which during in vivo vertebrate development gives rise to a variety of organs including liver, lungs, thyroid and pancreas; consequently efficient in vitro initiation of stem cell ... ...

    Abstract BACKGROUND: Definitive endoderm (DE) is one of the three germ layers which during in vivo vertebrate development gives rise to a variety of organs including liver, lungs, thyroid and pancreas; consequently efficient in vitro initiation of stem cell differentiation to DE cells is a prerequisite for successful cellular specification to subsequent DE-derived cell types [1, 2]. In this study we present a novel approach to rapidly and efficiently down regulate pluripotency genes during initiation of differentiation to DE cells by addition of dimethyl sulfoxide (DMSO) to Activin A-based culture medium and report its effects on the downstream differentiation to hepatocyte-like cells. MATERIALS AND METHODS: Human embryonic stem cells (hESC) were differentiated to DE using standard methods in medium supplemented with 100ng/ml of Activin A and compared to cultures where DE specification was additionally enhanced with different concentrations of DMSO. DE cells were subsequently primed to generate hepatic-like cells to investigate whether the addition of DMSO during formation of DE improved subsequent expression of hepatic markers. A combination of flow cytometry, real-time quantitative reverse PCR and immunofluorescence was applied throughout the differentiation process to monitor expression of pluripotency (POUF5/OCT4 & NANOG), definitive endoderm (SOX17, CXCR4 & GATA4) and hepatic (AFP & ALB) genes to generate differentiation stage-specific signatures. RESULTS: Addition of DMSO to the Activin A-based medium during DE specification resulted in rapid down regulation of the pluripotency genes OCT4 and NANOG, accompanied by an increase expression of the DE genes SOX17, CXCR4 and GATA4. Importantly, the expression level of ALB in DMSO-treated cells was also higher than in cells which were differentiated to the DE stage via standard Activin A treatment.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2015-01-01T00:00:00Z
    Publisher Public Library of Science (PLoS)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: Culturing Patient Cells for Cancer Immunotherapy: Challenges and Opportunities

    Levine, Bruce / Stephen Minger

    Science. 2013 Nov. 15, v. 342, no. 6160

    2013  

    Abstract: Immunotherapy that uses autologous cells has recently shown increasing success. This procedure, however, requires the generation of sufficient, high-quality cells for infusion into patients. Functionally closed, automated, single use cell culture systems ...

    Abstract Immunotherapy that uses autologous cells has recently shown increasing success. This procedure, however, requires the generation of sufficient, high-quality cells for infusion into patients. Functionally closed, automated, single use cell culture systems have significant advantages over the open, static systems that are commonly used. Keeping these systems small and efficient, while at the same time producing a sufficient number of cells is a challenge. Enabling high cell density culture is very important in order to minimize the total space required in a manufacturing or clinical development laboratory setting. However, achieving these high cell densities without compromising the quality of cells can be a challenge. This webinar will examine the conditions contributing to a healthy cell culture environment and explore ways to optimize high cell density culture. View the Webinar
    Keywords cell culture ; immunotherapy ; manufacturing ; patients
    Language English
    Dates of publication 2013-1115
    Size p. 875.
    Publishing place American Association for the Advancement of Science
    Document type Article
    ZDB-ID 128410-1
    ISSN 1095-9203 ; 0036-8075
    ISSN (online) 1095-9203
    ISSN 0036-8075
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  9. Article: Regenerative medicine in Parkinson's disease: generation of mesencephalic dopaminergic cells from embryonic stem cells.

    Taylor, Hannah / Minger, Stephen L

    Current opinion in biotechnology

    2005  Volume 16, Issue 5, Page(s) 487–492

    Abstract: Cell replacement therapy has been proposed as a means of replacing specific populations of cells lost through trauma, disease or ageing. Parkinson's disease is a progressive neurodegenerative disorder caused by the loss of midbrain dopaminergic neurons. ... ...

    Abstract Cell replacement therapy has been proposed as a means of replacing specific populations of cells lost through trauma, disease or ageing. Parkinson's disease is a progressive neurodegenerative disorder caused by the loss of midbrain dopaminergic neurons. Intrastriatal transplants of human foetal mesencephalic tissue in Parkinson's patients have demonstrated clinical efficacy, but the limited availability of tissue precludes systematic use of this treatment. Human embryonic stem cells are capable of unlimited self-renewal and can differentiate into cells representative of all three germ layers, including cells of the central nervous system. These cells may thus provide a relatively unlimited source of cells for transplantation, if appropriate differentiation protocols to generate highly enriched and specific populations of neural cells can be developed.
    MeSH term(s) Animals ; Cell Differentiation/physiology ; Cell Transplantation ; Dopamine/metabolism ; Embryo, Mammalian/cytology ; Embryo, Mammalian/metabolism ; Humans ; Mesencephalon/cytology ; Mice ; Models, Biological ; Neurons/cytology ; Neurons/transplantation ; Parkinson Disease/surgery ; Parkinson Disease/therapy ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; Regenerative Medicine/methods
    Chemical Substances Dopamine (VTD58H1Z2X)
    Language English
    Publishing date 2005-10
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1052045-4
    ISSN 1879-0429 ; 0958-1669
    ISSN (online) 1879-0429
    ISSN 0958-1669
    DOI 10.1016/j.copbio.2005.08.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

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  10. Article: Isolation, in vitro cultivation and characterisation of foetal liver cells.

    Wu, Yue / Shatapathy, Chetan C / Minger, Stephen L

    Methods in molecular biology (Clifton, N.J.)

    2009  Volume 481, Page(s) 181–192

    Abstract: Hepatocyte transplantation has recently become an efficient clinical method in the treatment of patients with metabolic liver diseases. The shortage of donor cells remains an obstacle to treat more patients. Foetal liver tissues may therefore be useful ... ...

    Abstract Hepatocyte transplantation has recently become an efficient clinical method in the treatment of patients with metabolic liver diseases. The shortage of donor cells remains an obstacle to treat more patients. Foetal liver tissues may therefore be useful as an alternative source of generating functional hepatocytes after in vitro culture and maturation.
    MeSH term(s) Animals ; Cell Culture Techniques/methods ; Cell Separation/methods ; Embryo, Mammalian/cytology ; Fetus/cytology ; Hepatocytes/cytology ; Hepatocytes/transplantation ; Humans ; Liver/cytology ; Rats ; Rats, Inbred F344 ; Tissue and Organ Harvesting/methods
    Language English
    Publishing date 2009
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ISSN 1064-3745
    ISSN 1064-3745
    DOI 10.1007/978-1-59745-201-4_15
    Database MEDical Literature Analysis and Retrieval System OnLINE

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