LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 9 of total 9

Search options

  1. Article ; Online: Interstitial cells of blood vessels.

    Pucovský, Vladimír

    TheScientificWorldJournal

    2010  Volume 10, Page(s) 1152–1168

    Abstract: Blood vessels are made up of several distinct cell types. Although it was originally thought that the tunica media of blood vessels was composed of a homogeneous population of fully differentiated smooth muscle cells, more recent data suggest the ... ...

    Abstract Blood vessels are made up of several distinct cell types. Although it was originally thought that the tunica media of blood vessels was composed of a homogeneous population of fully differentiated smooth muscle cells, more recent data suggest the existence of multiple smooth muscle cell subpopulations in the vascular wall. One of the cell types contributing to this heterogeneity is the novel, irregularly shaped, noncontractile cell with thin processes, termed interstitial cell, found in the tunica media of both veins and arteries. While the principal role of interstitial cells in veins seems to be pacemaking, the role of arterial interstitial cells is less clear. This review summarises the knowledge of the functional and structural properties of vascular interstitial cells accumulated so far, offers hypotheses on their physiological role, and proposes directions for future research.
    MeSH term(s) Animals ; Blood Vessels/cytology ; Humans ; Immunohistochemistry ; Muscle, Smooth, Vascular/cytology
    Language English
    Publishing date 2010-06-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 2075968-X
    ISSN 1537-744X ; 1537-744X
    ISSN (online) 1537-744X
    ISSN 1537-744X
    DOI 10.1100/tsw.2010.123
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Interstitial Cells of Blood Vessels

    Vladimír Pucovský

    The Scientific World Journal, Vol 10, Pp 1152-

    2010  Volume 1168

    Abstract: Blood vessels are made up of several distinct cell types. Although it was originally thought that the tunica media of blood vessels was composed of a homogeneous population of fully differentiated smooth muscle cells, more recent data suggest the ... ...

    Abstract Blood vessels are made up of several distinct cell types. Although it was originally thought that the tunica media of blood vessels was composed of a homogeneous population of fully differentiated smooth muscle cells, more recent data suggest the existence of multiple smooth muscle cell subpopulations in the vascular wall. One of the cell types contributing to this heterogeneity is the novel, irregularly shaped, noncontractile cell with thin processes, termed interstitial cell, found in the tunica media of both veins and arteries. While the principal role of interstitial cells in veins seems to be pacemaking, the role of arterial interstitial cells is less clear. This review summarises the knowledge of the functional and structural properties of vascular interstitial cells accumulated so far, offers hypotheses on their physiological role, and proposes directions for future research.
    Keywords Technology ; T ; Medicine ; R ; Science ; Q
    Language English
    Publishing date 2010-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Localisation, function and composition of primary Ca(2+) spark discharge region in isolated smooth muscle cells from guinea-pig mesenteric arteries.

    Pucovský, Vladimír / Bolton, Thomas B

    Cell calcium

    2006  Volume 39, Issue 2, Page(s) 113–129

    Abstract: Smooth muscle cells (SMCs) contain numerous calcium release domains, grouped into regions discharging as a single unit. Laser scanning confocal microscopy, voltage clamp and immunocytochemistry of single SMCs from small mesenteric arteries of guinea-pig ... ...

    Abstract Smooth muscle cells (SMCs) contain numerous calcium release domains, grouped into regions discharging as a single unit. Laser scanning confocal microscopy, voltage clamp and immunocytochemistry of single SMCs from small mesenteric arteries of guinea-pig were used to study the localisation, function and macromolecular composition of such calcium discharge regions (CDRs). Use of the Ca(2+)-sensitive fluorescent dye fluo-3 or fluo-4 with BODIPY TR-X ryanodine (BTR), a fluorescent derivative of ryanodine, showed spontaneous Ca(2+) sparks originating from regions stained by BTR, located immediately under the plasma membrane, in the arch formed by the sarcoplasmic reticulum surrounding the nucleus. Membrane depolarisation or application of noradrenaline or alpha,beta-methylene ATP, a P2X purinoceptor agonist, elicited Ca(2+) sparks from the same, spontaneous Ca(2+) spark-discharging region. The most active (primary) CDR accounted for nearly 60% of spontaneous transient outward currents at -40 mV and these were of significantly higher amplitude than the ones discharged by secondary CDRs. Immunocytochemical staining for type 1 IP(3) receptors, BK(Ca) channels, P2X(1) purinoceptors or alpha(1) adrenoceptors revealed their juxtaposition with BTR staining at the location typical of the primary CDR. These data suggest the existence of a primary calcium discharge region in SMCs; its position can be predicted from the cell's structure, it acts as a key region for the regulation of membrane potential via Ca(2+) sparks and is a potential link between the external, neurohumoral and the cell's internal, calcium signalling system.
    MeSH term(s) Animals ; Boron Compounds ; Calcium Channels/metabolism ; Calcium Signaling/physiology ; Fluorescent Dyes ; Guinea Pigs ; In Vitro Techniques ; Inositol 1,4,5-Trisphosphate Receptors ; Large-Conductance Calcium-Activated Potassium Channels/metabolism ; Male ; Membrane Potentials ; Mesenteric Arteries/cytology ; Mesenteric Arteries/metabolism ; Microscopy, Confocal ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/metabolism ; Myocytes, Smooth Muscle/metabolism ; Receptors, Adrenergic, alpha-1/metabolism ; Receptors, Cytoplasmic and Nuclear/metabolism ; Receptors, Purinergic P2/metabolism ; Receptors, Purinergic P2X ; Ryanodine/analogs & derivatives ; Ryanodine Receptor Calcium Release Channel/metabolism
    Chemical Substances 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene ; Boron Compounds ; Calcium Channels ; Fluorescent Dyes ; Inositol 1,4,5-Trisphosphate Receptors ; Large-Conductance Calcium-Activated Potassium Channels ; Receptors, Adrenergic, alpha-1 ; Receptors, Cytoplasmic and Nuclear ; Receptors, Purinergic P2 ; Receptors, Purinergic P2X ; Ryanodine Receptor Calcium Release Channel ; Ryanodine (15662-33-6)
    Language English
    Publishing date 2006-02
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 757687-0
    ISSN 1532-1991 ; 0143-4160
    ISSN (online) 1532-1991
    ISSN 0143-4160
    DOI 10.1016/j.ceca.2005.10.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1596: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Non-contractile cells with thin processes resembling interstitial cells of Cajal found in the wall of guinea-pig mesenteric arteries.

    Pucovský, Vladimír / Moss, Ray F / Bolton, Thomas B

    The Journal of physiology

    2003  Volume 552, Issue Pt 1, Page(s) 119–133

    Abstract: Arterial interstitial cells of Cajal (ICC)-like cells (AIL cells) with a multipolar, irregular, elongated shape and with numerous thin (often less than 1 microm), sometimes branching, processes with lengths up to approximately 60 microm were isolated ... ...

    Abstract Arterial interstitial cells of Cajal (ICC)-like cells (AIL cells) with a multipolar, irregular, elongated shape and with numerous thin (often less than 1 microm), sometimes branching, processes with lengths up to approximately 60 microm were isolated enzymatically from 1st to 7th order branches of guinea-pig mesenteric artery. Some of the processes of AIL cells were growing (average speed approximately 0.15 microm min-1) and their growth was blocked by 10 microM latrunculin B, an inhibitor of actin polymerisation. Staining with BODIPY phalloidin, a fluorescent dye selective for F-actin, showed the presence of F-actin in the processes of AIL cells. Voltage clamp of single AIL cells revealed an inward current that was four times more dense than in myocytes and was abolished by 10 microM nicardipine, and an outward current carried exclusively by potassium ions that was reduced by 1 mM 4-aminopyridine and/or 100 nM iberiotoxin but unaffected by 10 nM dendrotoxin-K. Imaging of intracellular ionised calcium with fluo-4 using a laser scanning confocal microscope showed local or global calcium transients lasting several seconds in approximately 28 % of AIL cells. When membrane current was recorded simultaneously, the calcium transients were found to correspond to long-lasting transient outward currents, which occurred at potentials positive to -40 mV. Unlike myocytes, AIL cells did not contract in response to 1 mM caffeine or 5 microM noradrenaline, although they responded with a [Ca2+]i increase. The segments of intact arteries did not stain for c-kit, a marker of ICCs. Single AIL cells stained positive for vimentin, desmin and smooth muscle myosin. The presence of ICC-like cells is demonstrated for the first time in the media of resistance arteries.
    MeSH term(s) Animals ; Caffeine/pharmacology ; Calcium/metabolism ; Cell Size ; Electric Capacitance ; Guinea Pigs ; Immunohistochemistry ; Male ; Membrane Potentials/drug effects ; Membrane Potentials/physiology ; Mesenteric Arteries/cytology ; Mesenteric Arteries/innervation ; Mesenteric Arteries/physiology ; Microscopy, Electron ; Myocytes, Smooth Muscle/chemistry ; Myocytes, Smooth Muscle/ultrastructure ; Nerve Endings/chemistry ; Nerve Endings/physiology ; Nerve Endings/ultrastructure ; Norepinephrine/pharmacology ; Patch-Clamp Techniques ; Phosphodiesterase Inhibitors/pharmacology ; Proto-Oncogene Proteins c-kit/analysis ; Smooth Muscle Myosins/analysis ; Vasoconstriction/physiology ; Vasoconstrictor Agents/pharmacology ; Vimentin/analysis
    Chemical Substances Phosphodiesterase Inhibitors ; Vasoconstrictor Agents ; Vimentin ; Caffeine (3G6A5W338E) ; Proto-Oncogene Proteins c-kit (EC 2.7.10.1) ; Smooth Muscle Myosins (EC 3.6.1.-) ; Calcium (SY7Q814VUP) ; Norepinephrine (X4W3ENH1CV)
    Language English
    Publishing date 2003-10-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3115-x
    ISSN 1469-7793 ; 0022-3751
    ISSN (online) 1469-7793
    ISSN 0022-3751
    DOI 10.1113/jphysiol.2003.046243
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.65: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Role of intracellular stores in the regulation of rhythmical [Ca2+]i changes in interstitial cells of Cajal from rabbit portal vein.

    Harhun, Maksym / Gordienko, Dmitri / Kryshtal, Dmytro / Pucovský, Vladimír / Bolton, Thomas

    Cell calcium

    2006  Volume 40, Issue 3, Page(s) 287–298

    Abstract: Interstitial cells of Cajal (ICCs) freshly isolated from rabbit portal vein and loaded with the Ca(2+)-sensitive indicator fluo-3 revealed rhythmical [Ca(2+)](i) changes occurring at 0.02-0.1 Hz. Each increase in [Ca(2+)](i) originated from a discrete ... ...

    Abstract Interstitial cells of Cajal (ICCs) freshly isolated from rabbit portal vein and loaded with the Ca(2+)-sensitive indicator fluo-3 revealed rhythmical [Ca(2+)](i) changes occurring at 0.02-0.1 Hz. Each increase in [Ca(2+)](i) originated from a discrete central region of the ICC and propagated as a [Ca(2+)](i) wave towards the cell periphery, but usually became attenuated before reaching the ends of the cell. In about 40% of ICCs each rhythmical change in [Ca(2+)](i) consisted of an initial [Ca(2+)](i) increase (phase 1) followed by a faster rise in [Ca(2+)](i) (phase 2) and then a decrease in [Ca(2+)](i) (phase 3); the frequency correlated with the rate of rise of [Ca(2+)](i) during phase 1, but not with the peak amplitude. Rhythmical [Ca(2+)](i) changes persisted in nicardipine, but were abolished in Ca(2+)-free solution as well as by SK&F96365, cyclopiazonic acid, thapsigargin, 2-APB, xestospongin C or ryanodine. Intracellular Ca(2+) stores visualised with the low-affinity Ca(2+) indicator fluo-3FF were found to be enriched with ryanodine receptors (RyRs) detected with BODIPY TR-X ryanodine. Rhythmical [Ca(2+)](i) changes originated from a perinuclear S/ER element showing the highest RyR density. Immunostaining with anti-TRPC3,6,7 antibodies revealed the expression of these channel proteins in the ICC plasmalemma. This suggests that these rhythmical [Ca(2+)](i) changes, a key element of ICC pacemaking activity, result from S/ER Ca(2+) release which is mediated via RyRs and IP(3) receptors and is modulated by the activity of S/ER-Ca(2+)-ATPase and TRP channels but not by L-type Ca(2+) channels.
    MeSH term(s) Animals ; Calcium/metabolism ; Cells, Cultured ; Endoplasmic Reticulum/chemistry ; Endoplasmic Reticulum/metabolism ; Microscopy, Confocal ; Muscle Contraction ; Muscle, Smooth/physiology ; Periodicity ; Portal Vein/cytology ; Portal Vein/metabolism ; Portal Vein/physiology ; Rabbits ; Ryanodine Receptor Calcium Release Channel/analysis ; Sarcoplasmic Reticulum/chemistry ; Sarcoplasmic Reticulum/metabolism ; Transient Receptor Potential Channels/analysis
    Chemical Substances Ryanodine Receptor Calcium Release Channel ; Transient Receptor Potential Channels ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2006-09
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 757687-0
    ISSN 1532-1991 ; 0143-4160
    ISSN (online) 1532-1991
    ISSN 0143-4160
    DOI 10.1016/j.ceca.2006.04.018
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1596: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Effect of nitric oxide donors and noradrenaline on Ca2+ release sites and global intracellular Ca2+ in myocytes from guinea-pig small mesenteric arteries.

    Pucovský, Vladimír / Gordienko, Dmitri V / Bolton, Thomas B

    The Journal of physiology

    2002  Volume 539, Issue Pt 1, Page(s) 25–39

    Abstract: In smooth muscle the spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) occurs at preferred locations called frequent discharge sites (FDSs) giving rise to localized intracellular Ca2+ transients (Ca2+ sparks). Laser scanning confocal ... ...

    Abstract In smooth muscle the spontaneous Ca2+ release from the sarcoplasmic reticulum (SR) occurs at preferred locations called frequent discharge sites (FDSs) giving rise to localized intracellular Ca2+ transients (Ca2+ sparks). Laser scanning confocal microscopy of fluo-3-loaded single myocytes freshly isolated from small mesenteric arteries of guinea-pig was used to investigate the action of nitric oxide (NO) donors and noradrenaline on the position and activity of FDSs and on global intracellular Ca2+ concentration ([Ca2+]i). In 8 % of cells 'microsparks', Ca2+ release events smaller in duration, spread and amplitude than Ca2+ sparks were observed. The location of the initiation point of Ca2+ sparks observed during line-scan imaging was found to 'jitter' by +/- 0.41 microm. However, the general position of an FDS within the cell did not change; most FDSs were close (within 1.2 +/- 0.1 microm) to the cell membrane and often multiple FDSs occurred in one confocal plane of the cell. In the resting state, NO donors S-nitroso-N-acetylpenicillamine (SNAP; 50 microM) and sodium nitroprusside (SNP; 100 microM) did not change the general position of FDSs and slightly depressed their activity, but did not affect the global [Ca2+]i significantly. Application of noradrenaline (1-10 microM) increased Ca2+ spark frequency at existing FDS(s) leading to a Ca2+ wave. The increase in FDS activity and in global [Ca2+]i produced by noradrenaline were inhibited by the presence of SNAP or SNP but not by 8-bromoguanosine cyclic 3',5'-monophosphate (8-Br-cGMP; 100 microM). In the presence of 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), inhibitor of soluble guanylate cyclase, SNAP and SNP still exerted their effects on the noradrenaline response. These results suggest that SNAP and SNP inhibit the noradrenaline-evoked rise in global [Ca2+]i by a cGMP-independent mechanism and that part of this effect is due to inhibition of the activity of FDSs; moreover, only the activity, but not the position, of FDSs is changed by either stimulant or inhibitory substances.
    MeSH term(s) Animals ; Calcium/metabolism ; Cyclic GMP/analogs & derivatives ; Cyclic GMP/pharmacology ; Enzyme Inhibitors/pharmacology ; Guanylate Cyclase/antagonists & inhibitors ; Guanylate Cyclase/physiology ; Guinea Pigs ; Intracellular Membranes/metabolism ; Male ; Mesenteric Arteries/cytology ; Mesenteric Arteries/drug effects ; Mesenteric Arteries/metabolism ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/metabolism ; Nitric Oxide Donors/pharmacology ; Nitroprusside/pharmacology ; Norepinephrine/pharmacology ; Oxadiazoles/pharmacology ; Quinoxalines/pharmacology ; S-Nitroso-N-Acetylpenicillamine/pharmacology
    Chemical Substances 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one ; Enzyme Inhibitors ; Nitric Oxide Donors ; Oxadiazoles ; Quinoxalines ; Nitroprusside (169D1260KM) ; 8-bromocyclic GMP (31356-94-2) ; S-Nitroso-N-Acetylpenicillamine (79032-48-7) ; Guanylate Cyclase (EC 4.6.1.2) ; Cyclic GMP (H2D2X058MU) ; Calcium (SY7Q814VUP) ; Norepinephrine (X4W3ENH1CV)
    Language English
    Publishing date 2002-02-15
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3115-x
    ISSN 1469-7793 ; 0022-3751
    ISSN (online) 1469-7793
    ISSN 0022-3751
    DOI 10.1113/jphysiol.2001.012978
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.65: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: Effects of various reactive oxygen species on the guinea pig trachea and its epithelium.

    Mátyás, Stefan / Pucovský, Vladimir / Bauer, Viktor

    Japanese journal of pharmacology

    2002  Volume 88, Issue 3, Page(s) 270–278

    Abstract: Reactive oxygen species (ROS) are key factors playing important roles in tissue damage of airways under different pathological conditions. Effects of ROS (superoxide anion, H2O2 and hydroxyl radical) were recorded on isometric tension of intact and ... ...

    Abstract Reactive oxygen species (ROS) are key factors playing important roles in tissue damage of airways under different pathological conditions. Effects of ROS (superoxide anion, H2O2 and hydroxyl radical) were recorded on isometric tension of intact and epithelium denuded, not precontracted guinea pig trachea. Superoxide anion was produced by xanthine/xanthine oxidase and hydroxyl radical either by FeSO4/H2O2 or FeSO4/ascorbic acid. In intact preparations, the muscle tension was unaffected by superoxide anion, while H2O2 and hydroxyl radical produced a biphasic response, contraction followed by relaxation. Both the amplitude and duration of contractions evoked by H2O2 were larger than those caused by hydroxyl radical producing systems. On denuded tracheal strips, superoxide anion elicited also a biphasic response, and the H2O2 and hydroxyl radical produced contractions were of higher amplitude and of longer duration than in intact tissues. Indomethacin pretreatment enhanced or slightly reduced the amplitude of contractions evoked by both H2O2 and hydroxyl radical on the intact and denuded preparations, respectively. Moreover, the duration of contractions of the trachea induced by oxidative systems was prolonged. Indomethacin did not affect the action of superoxide anion on the intact tissues and reduced the amplitude of the biphasic response on denuded ones. Nordihydroguaiaretic acid pretreatment did not alter the responses elicited by ROS in intact preparations and reduced their action on the denuded ones. Our results suggest that a) various ROS contract tracheal smooth muscle with simultaneous release of epithelium derived relaxing factors, b) epithelium possesses superoxide anion scavenging capacity which is high enough to protect smooth muscle from its actions, and c) cyclooxygenase products participate in relaxation and lipoxygenase products in contraction caused by ROS in the guinea pig trachea.
    MeSH term(s) Animals ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Arachidonic Acid/metabolism ; Cytochrome c Group/metabolism ; Epithelium/physiology ; Guinea Pigs ; Hydrogen Peroxide/toxicity ; In Vitro Techniques ; Indomethacin/pharmacology ; Male ; Muscle Tonus/drug effects ; Muscle, Smooth/metabolism ; Muscle, Smooth/physiology ; Oxidants/toxicity ; Reactive Oxygen Species/metabolism ; Superoxides/metabolism ; Superoxides/toxicity ; Trachea/physiology ; Xanthines/metabolism
    Chemical Substances Anti-Inflammatory Agents, Non-Steroidal ; Cytochrome c Group ; Oxidants ; Reactive Oxygen Species ; Xanthines ; Superoxides (11062-77-4) ; Arachidonic Acid (27YG812J1I) ; Hydrogen Peroxide (BBX060AN9V) ; Indomethacin (XXE1CET956)
    Language English
    Publishing date 2002-03
    Publishing country Japan
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218222-1
    ISSN 1347-3506 ; 0021-5198
    ISSN (online) 1347-3506
    ISSN 0021-5198
    DOI 10.1254/jjp.88.270
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 237: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: Electrophysiological and molecular identification of voltage-gated sodium channels in murine vascular myocytes.

    Saleh, Sohag / Yeung, Shuk Yin M / Prestwich, Sally / Pucovsky, Vladimír / Greenwood, Iain

    The Journal of physiology

    2005  Volume 568, Issue Pt 1, Page(s) 155–169

    Abstract: A voltage-gated Na+ current was characterised in freshly dissociated mouse portal vein (PV) smooth muscle myocytes. The current was found superimposed upon the relatively slow L-type Ca2+ current and was resistant to conventional Ca2+ channel blockers ... ...

    Abstract A voltage-gated Na+ current was characterised in freshly dissociated mouse portal vein (PV) smooth muscle myocytes. The current was found superimposed upon the relatively slow L-type Ca2+ current and was resistant to conventional Ca2+ channel blockers but was abolished by external Na+ replacement and tetrodotoxin (TTX, 1 microM). The molecular identity of the channel responsible for this conductance was determined by RT-PCR where only the transcripts for Na+ channel genes SCN7a, 8a and 9a were detected. The presence of the protein counterparts to the SCN8a and 9a genes (NaV1.6 and NaV1.7, respectively) on the individual smooth muscle myocytes were confirmed in immunocytochemistry, which showed diffuse staining around a predominantly plasmalemmal location. TTX inhibited the action potential in individual myocytes generated in the current clamp mode but isometric tissue tension experiments revealed that TTX (1 and 5 microM) had no effect on the inherent mouse PV rhythmicity. However, the Na+ channel opener veratridine (10 and 50 microM) significantly increased the length of contraction and the interval between contractions. This effect was not influenced by pre-incubation with atropine, prazosin and propranolol, but was reversed by TTX (1 microM) and completely abolished by nicardipine (1 microM). Furthermore, preincubation with the reverse-mode Na+-Ca2+ exchange blocker KB-R7943 (10 microM) also inhibited the veratridine response. We have established for the first time the molecular identity of the voltage-gated Na+ channel in freshly dispersed smooth muscle cells and have shown that these channels can modulate contractility through a novel mechanism of action possibly involving reverse mode Na+-Ca2+ exchange.
    MeSH term(s) Action Potentials/drug effects ; Animals ; Calcium/metabolism ; Calcium Channel Blockers/pharmacology ; Dose-Response Relationship, Drug ; Female ; Ion Channel Gating/drug effects ; Mice ; Mice, Inbred BALB C ; Muscle Contraction/drug effects ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/metabolism ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism ; NAV1.6 Voltage-Gated Sodium Channel ; NAV1.7 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins/analysis ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; Nicardipine/pharmacology ; Portal Vein/drug effects ; Portal Vein/physiology ; Sodium/metabolism ; Sodium Channels/analysis ; Sodium Channels/genetics ; Sodium Channels/metabolism ; Sodium-Calcium Exchanger/antagonists & inhibitors ; Sodium-Calcium Exchanger/metabolism ; Tetrodotoxin/pharmacology ; Thiourea/analogs & derivatives ; Thiourea/pharmacology ; Veratridine/pharmacology
    Chemical Substances 2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate ; Calcium Channel Blockers ; NAV1.6 Voltage-Gated Sodium Channel ; NAV1.7 Voltage-Gated Sodium Channel ; Nerve Tissue Proteins ; Scn8a protein, mouse ; Scn9a protein, mouse ; Sodium Channels ; Sodium-Calcium Exchanger ; Tetrodotoxin (4368-28-9) ; Veratridine (71-62-5) ; Sodium (9NEZ333N27) ; Nicardipine (CZ5312222S) ; Thiourea (GYV9AM2QAG) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2005-10-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3115-x
    ISSN 1469-7793 ; 0022-3751
    ISSN (online) 1469-7793
    ISSN 0022-3751
    DOI 10.1113/jphysiol.2005.090951
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.65: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article: Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell.

    Pucovský, Vladimír / Harhun, Maksym I / Povstyan, Oleksandr V / Gordienko, Dmitri V / Moss, Ray F / Bolton, Thomas B

    Journal of cellular and molecular medicine

    2007  Volume 11, Issue 4, Page(s) 764–775

    Abstract: This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of ... ...

    Abstract This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of guinea-pig mesenteric arteries and single AIL cells freshly isolated from them were used. Confocal imaging of immunostained cells or segments and electron microscopy of artery segments were used to test for the presence and cellular localization of selected markers, and to localize AIL cells in intact artery segments. AIL cells were negative for PGP9.5, a neural marker, and for von Willebrand factor (vWF), an endothelial cell marker. They were positive for smooth muscle alpha-actin and smooth muscle myosin heavy chain (SM-MHC), but expressed only a small amount of smoothelin, a marker of contractile smooth muscle cells (SMC), and of myosin light chain kinase (MLCK), a critical enzyme in the regulation of smooth muscle contraction. Cell isolation in the presence of latrunculin B, an actin polymerization inhibitor, did not cause the disappearance of AIL cells from cell suspension. The fluorescence of basal lamina protein collagen IV was comparable between the AIL cells and the vascular SMCs and the fluorescence of laminin was higher in AIL cells compared to vascular SMCs. Moreover, cells with thin processes were found in the tunica media of small resistance arteries using transmission electron microscopy. The results suggest that AIL cells are immature or phenotypically modulated vascular SMCs constitutively present in resistance arteries.
    MeSH term(s) Animals ; Artifacts ; Biomarkers/metabolism ; Cell Separation ; Cytoskeletal Proteins/metabolism ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Guinea Pigs ; Mesenteric Arteries/cytology ; Mesenteric Arteries/enzymology ; Mesenteric Arteries/ultrastructure ; Microscopy, Fluorescence ; Muscle Contraction ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/physiology ; Myosin-Light-Chain Kinase/metabolism ; Neurons/metabolism ; Phenotype ; Ubiquitin Thiolesterase/metabolism ; Vascular Resistance ; von Willebrand Factor/metabolism
    Chemical Substances Biomarkers ; Cytoskeletal Proteins ; von Willebrand Factor ; Myosin-Light-Chain Kinase (EC 2.7.11.18) ; Ubiquitin Thiolesterase (EC 3.4.19.12)
    Language English
    Publishing date 2007-08-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2074559-X
    ISSN 1582-4934 ; 1582-1838 ; 1582-4934
    ISSN (online) 1582-4934
    ISSN 1582-1838 ; 1582-4934
    DOI 10.1111/j.1582-4934.2007.00066.x
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5752: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top