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Article ; Online: Comparing the Predicted versus Realized Rate of Adaptation of

Peschel, Anna R / Shaw, Ruth G

2023 Volume 203, Issue 1, Page(s) 14–27

Abstract: AbstractFisher's fundamental theorem of natural selection (FTNS) can be used in a quantitative genetics framework to predict the rate of adaptation in populations. Here, we estimated the capacity for a wild population of the annual ... ...

Abstract	AbstractFisher's fundamental theorem of natural selection (FTNS) can be used in a quantitative genetics framework to predict the rate of adaptation in populations. Here, we estimated the capacity for a wild population of the annual legume
MeSH term(s)	Chamaecrista/physiology ; Climate Change ; Selection, Genetic ; Population Dynamics ; Seeds ; Adaptation, Physiological
Language	English
Publishing date	2023-12-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	207092-3
ISSN	1537-5323 ; 0003-0147
ISSN (online)	1537-5323
ISSN	0003-0147
DOI	10.1086/727507
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Article: Peripheral blood stem cell (PBSC) mobilization with chemotherapy followed by sequential IL-3 and G-CSF administration in extensively pretreated patients.

Kolbe, K / Peschel, C / Rupilius, B / Després, D / Burger, K / Sklenar, I / Färber, L / Huber, C / Derigs, H G

Bone marrow transplantation

1997 Volume 20, Issue 12, Page(s) 1027–1032

Abstract: ... for 5 consecutive days and G-CSF (10 microg/kg) until PBSC collection or neutrophil recovery. Patients ... Twelve patients had prior unsuccessful trial of PBSC mobilization with chemotherapy followed by G-CSF ... recovery was seen in all of them. In conclusion, the sequential administration of IL-3 followed by G-CSF ...

Abstract	Extensive pretreatment has been identified as a significant risk factor for failure of sufficient PBSC mobilization. From published data and our own experience we defined pretreatment variables which render patients at risk for not collecting at least 2.5 x 10(6) CD34-positive cells per kg bodyweight (BW). These variables were previous unsuccessful PBSC mobilization trial, previous large field radiotherapy, four or more cycles of myelosuppressive chemotherapy regimens, and combinations of extended field radiotherapy plus chemotherapy. Based on these inclusion criteria we treated 19 patients with disease-specific conventional-dose chemotherapy followed by sequential subcutaneous administration of IL-3 (5 microg/kg BW) for 5 consecutive days and G-CSF (10 microg/kg) until PBSC collection or neutrophil recovery. Patients were 10 males and nine females with a median age of 43 years. Diagnoses were non-Hodgkin's lymphoma n = 5, Hodgkin's disease n = 2, multiple myeloma n = 2, CML n = 4, AML n = 4 and testicular cancer n = 2. Twelve patients had prior unsuccessful trial of PBSC mobilization with chemotherapy followed by G-CSF. Except for mobilization chemotherapy-related neutropenic fever, no major toxicities (WHO grade > or = 2) were observed. Growth factors were well tolerated. Collection of at least 2.5 x 10(6) CD34-positive cells per kg BW was possible in 11 out of 19 patients (58%). In five out of 12 patients with a previous unsuccessful trial of PBSC mobilization, the study regimen mobilized sufficient CD34-positive cells. Nine patients went on to high-dose chemotherapy followed by autologous PBSC transplantation. Prompt hematologic recovery was seen in all of them. In conclusion, the sequential administration of IL-3 followed by G-CSF after conventional-dose chemotherapy allows successful PBSC collection in the majority of extensively pretreated patients.
MeSH term(s)	Adult ; Antineoplastic Combined Chemotherapy Protocols/administration & dosage ; Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Antineoplastic Combined Chemotherapy Protocols/pharmacology ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Blood Cell Count ; Combined Modality Therapy ; Drug Synergism ; Female ; Fever/chemically induced ; Germinoma/blood ; Germinoma/drug therapy ; Germinoma/therapy ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Granulocyte Colony-Stimulating Factor/adverse effects ; Granulocyte Colony-Stimulating Factor/pharmacology ; Hematologic Neoplasms/blood ; Hematologic Neoplasms/drug therapy ; Hematologic Neoplasms/radiotherapy ; Hematologic Neoplasms/therapy ; Hematopoietic Stem Cell Mobilization/methods ; Hematopoietic Stem Cell Transplantation/methods ; Humans ; Interleukin-3/administration & dosage ; Interleukin-3/adverse effects ; Interleukin-3/pharmacology ; Male ; Middle Aged ; Neutropenia/chemically induced ; Pain/chemically induced ; Remission Induction ; Risk Factors ; Salvage Therapy ; Seminoma/blood ; Seminoma/drug therapy ; Seminoma/radiotherapy ; Seminoma/therapy ; Testicular Neoplasms/blood ; Testicular Neoplasms/drug therapy ; Testicular Neoplasms/radiotherapy ; Testicular Neoplasms/therapy
Chemical Substances	Interleukin-3 ; Granulocyte Colony-Stimulating Factor (143011-72-7)
Language	English
Publishing date	1997-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632854-4
ISSN	1476-5365 ; 0268-3369 ; 0951-3078
ISSN (online)	1476-5365
ISSN	0268-3369 ; 0951-3078
DOI	10.1038/sj.bmt.1701019
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Article ; Online: Traumatic fractures in an early 19th century museum skeleton suggest the homicide of an old Munich character: the history of "Finessensepperl" (Finesse Joseph).

Nerlich, Andreas G / Panzer, Stephanie / Lehn, Christine / Friederichs, Jan / Peschel, Oliver K

Forensic science, medicine, and pathology

2024

Abstract: The well preserved skeleton of Joseph Huber, a very well-known historical character of the 19th century Munich, also nicknamed "Finessen-Sepperl", is the starting point of the reconstruction of life and death of this historical individual. He was known ... ...

Abstract	The well preserved skeleton of Joseph Huber, a very well-known historical character of the 19th century Munich, also nicknamed "Finessen-Sepperl", is the starting point of the reconstruction of life and death of this historical individual. He was known as a postilion d´amour (love's messenger) of the Royal Bavarian capital with numerous comments and anecdotes and a few biographical sketches that indicate he remained well until the last few years of his life where requests for his duties lessened. The skeleton shows a small-sized male individual with almost complete loss of teeth, but otherwise very well-mineralized bone, having suffered from three episodes of trauma - an old-healed incomplete femoral neck fracture leading to severe osteoarthrosis, a clavicle fracture of the medial third with a few weeks old callus formation, and fresh serial rib fractures along with severe skull trauma with fractures of the os temporale and petrosum, presumably leading to intracranial bleeding and finally death. The type and distribution of these latter two injuries are in agreement with a murderous attack - which was retrospectively reported several years after his death, while the old-healed femoral neck fracture may have caused reduction in Joseph´s walking activities but not reduced requests for his services. Paleopathology not only identifies the terminal decline, but also previous diseases of this Old Bavarian character and thereby completes his story.
Language	English
Publishing date	2024-04-12
Publishing country	United States
Document type	Journal Article
ZDB-ID	2195904-3
ISSN	1556-2891 ; 1547-769X
ISSN (online)	1556-2891
ISSN	1547-769X
DOI	10.1007/s12024-024-00811-w
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Article: Analysis of the Staphylococcus epidermidis genes epiF, -E, and -G involved in epidermin immunity.

Peschel, A / Götz, F

Journal of bacteriology

1996 Volume 178, Issue 2, Page(s) 531–536

Abstract: ... of a 2.6-kb DNA fragment revealed three open reading frames, epiF, -E, and -G, which may form an operon ... G resulted in the complete loss of the immunity phenotype. An epidermin-sensitive S. epidermidis ... production was approximately fivefold higher. The proteins EpiF, -E, and -G are similar in deduced sequence ...

Abstract	The lantibiotic epidermin is produced by Staphylococcus epidermidis Tü3298. The known genes involved in epidermin biosynthesis and regulation are organized as operons (epiABCD and epiQP) that are encoded on the 54-kb plasmid pTü32. Here we describe the characterization of a DNA region that mediates immunity and increased epidermin production, located upstream of the structural gene epiA. The sequence of a 2.6-kb DNA fragment revealed three open reading frames, epiF, -E, and -G, which may form an operon. In the cloning host Staphylococcus carnosus, the three genes mediated an increased tolerance to epidermin, and the highest level of immunity (sevenfold) was achieved with S. carnosus carrying epiFEG and epiQ. The promoter of the first gene, epiF, responded to the activator protein EpiQ and contained a palindromic sequence similar to the EpiQ binding site of the epiA promoter, which is also activated by EpiQ. Inactivation of epiF, -E, or -G resulted in the complete loss of the immunity phenotype. An epidermin-sensitive S. epidermidis Tü3298 mutant was complemented by a DNA fragment containing all three genes. When the epiFEG genes were cloned together with plasmid pTepi14, containing the biosynthetic genes epiABCDQP, the level of epidermin production was approximately fivefold higher. The proteins EpiF, -E, and -G are similar in deduced sequence and proposed structure to the components of various ABC transporter systems. EpiF is a hydrophilic protein with conserved ATP-binding sites, while EpiE and -G have six alternating hydrophobic regions and very likely constitute the integral membrane domains. When EpiF was overproduced in S. carnosus, it was at least partially associated with the cytoplasmic membrane. A potential mechanism for how EpiFEG mediates immunity is discussed.
MeSH term(s)	ATP-Binding Cassette Transporters/biosynthesis ; ATP-Binding Cassette Transporters/chemistry ; ATP-Binding Cassette Transporters/genetics ; Amino Acid Sequence ; Anti-Bacterial Agents/biosynthesis ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/biosynthesis ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacteriocins ; Base Sequence ; Cloning, Molecular ; Drug Resistance, Microbial/genetics ; Genes, Bacterial/genetics ; Genes, Bacterial/physiology ; Membrane Proteins/genetics ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Molecular Weight ; Operon/genetics ; Peptides ; Promoter Regions, Genetic/genetics ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Staphylococcus/drug effects ; Staphylococcus epidermidis/drug effects ; Staphylococcus epidermidis/genetics
Chemical Substances	ATP-Binding Cassette Transporters ; Anti-Bacterial Agents ; Bacterial Proteins ; Bacteriocins ; EpiE protein, Staphylococcus epidermidis ; EpiF protein, Staphylococcus epidermidis ; EpiG protein, Staphylococcus epidermidis ; Membrane Proteins ; Peptides ; epidermin (99165-17-0)
Language	English
Publishing date	1996-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2968-3
ISSN	1098-5530 ; 0021-9193
ISSN (online)	1098-5530
ISSN	0021-9193
DOI	10.1128/jb.178.2.531-536.1996
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Article: Multisite Phosphorylation of Nuclear Interaction Partner of ALK (NIPA) at G₂/M Involves Cyclin B1/Cdk1

Bassermann, Florian / von Klitzing, Christine / Illert, Anna Lena / Münch, Silvia / Morris, Stephan W / Pagano, Michele / Peschel, Christian / Duyster, Justus

Journal of biological chemistry. 2007 June 1, v. 282, no. 22

2007

Abstract: ... whereas phosphorylation of NIPA in late G₂ phase and mitosis inactivates the complex to allow for accumulation of cyclin ... implicated in the phosphorylation process at G₂/M within this region. Moreover, we found cyclin B1/Cdk1 ...

Abstract	Nuclear interaction partner of ALK (NIPA) is an F-box-containing protein that defines a nuclear skp1 cullin F-box (SCF)-type ubiquitin E3 ligase (SCFNIPA) implicated in the regulation of mitotic entry. The SCFNIPA complex targets nuclear cyclin B1 for ubiquitination in interphase, whereas phosphorylation of NIPA in late G₂ phase and mitosis inactivates the complex to allow for accumulation of cyclin B1. Here, we identify the region of NIPA that mediates binding to its substrate cyclin B1. In addition to the recently described serine residue 354, we specify 2 new residues, Ser-359 and Ser-395, implicated in the phosphorylation process at G₂/M within this region. Moreover, we found cyclin B1/Cdk1 to phosphorylate NIPA at Ser-395 in mitosis. Mutation of both Ser-359 and Ser-395 impaired effective inactivation of the SCFNIPA complex, resulting in reduced levels of mitotic cyclin B1. These data are compatible with a process of sequential NIPA phosphorylation where cyclin B1/Cdk1 amplifies phosphorylation of NIPA once an initial phosphorylation event has dissociated the SCFNIPA complex. Thus, cyclin B1/Cdk1 may contribute to the regulation of its own abundance in early mitosis.
Language	English
Dates of publication	2007-0601
Size	p. 15965-15972.
Publishing place	American Society for Biochemistry and Molecular Biology
Document type	Article
ZDB-ID	2997-x
ISSN	1083-351X ; 0021-9258
ISSN (online)	1083-351X
ISSN	0021-9258
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Article: Serum omentin-1 is correlated with the severity of liver disease in patients with chronic hepatitis C.

Peschel, Georg / Weigand, Kilian / Grimm, Jonathan / Müller, Martina / Buechler, Christa

World journal of hepatology

2023 Volume 15, Issue 12, Page(s) 1315–1324

Abstract: Background: Patients with chronic hepatitis C virus (HCV) infection have increased serum omentin-1. Omentin-1 is an anti-inflammatory adipokine, and higher levels may be a direct effect of HCV infection. Successful elimination of HCV by direct acting ... ...

Abstract	Background: Patients with chronic hepatitis C virus (HCV) infection have increased serum omentin-1. Omentin-1 is an anti-inflammatory adipokine, and higher levels may be a direct effect of HCV infection. Successful elimination of HCV by direct acting antivirals almost normalized circulating levels of various molecules with a role in inflammation. Aim: To evaluate the effect of HCV infection on serum omentin-1, serum omentin-1 levels of HCV patients were measured before therapy and at 12 wk after therapy end. Associations of serum omentin-1 with parameters of inflammation and liver function were explored at both time points. Serum omentin-1 levels of patients with and without liver cirrhosis, which was defined by ultrasound or the fibrosis-4 (FIB-4) score, were compared. Methods: Serum omentin-1 levels were measured by enzyme-linked immunosorbent assay in 84 chronic HCV patients before therapy and at 12 wk after therapy end where sustained virological response 12 (SVR12) was achieved in all patients. Serum omentin-1 of 14 non-infected controls was measured in parallel. Results: In patients with chronic HCV, serum omentin-1 levels were not related to viral load or viral genotype. HCV patients with liver steatosis and HCV patients with diabetes had serum omentin-1 levels comparable to patients not suffering from these conditions. Serum omentin-1 levels at SVR12 were similar in comparison to pretreatment levels. In addition, serum levels did not differ between HCV-infected patients and non-infected controls. Serum omentin-1 levels did not correlate with leukocyte count or C-reactive protein. Positive correlations of serum omentin-1 with bilirubin and the model for end-stage liver disease score (MELD) were detected before therapy and at SVR12 in the whole cohort. Bilirubin and the MELD score also positively correlated with serum omentin-1 levels in the subgroup of patients with ultrasound diagnosed liver cirrhosis before therapy. At SVR12, serum omentin-1 levels of patients with liver cirrhosis negatively correlated with albumin. Before therapy start, patients with high FIB-4 scores had increased serum omentin-1 in comparison to patients with a low score. Serum omentin-1 levels of patients with liver cirrhosis defined by ultrasound were increased at baseline and at SVR12. Conclusion: Present study showed that liver cirrhosis, but not HCV infection per se, is related to elevated serum omentin-1 levels.
Language	English
Publishing date	2023-12-11
Publishing country	United States
Document type	Journal Article
ZDB-ID	2573703-X
ISSN	1948-5182
ISSN	1948-5182
DOI	10.4254/wjh.v15.i12.1315
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Article: Preliminary results of stem cell mobilization in chronic myeloid leukemia with a moderate intensity chemotherapy regimen and G-CSF or G-CSF plus IL-3.

Aulitzky, W E / Neubauer, A / Kolbe, K / Schneller, F / Busemann, C / Schleiermacher, E / Peschel, C / Siegert, W / Huber, C / Huhn, D

Bone marrow transplantation

1996 Volume 17 Suppl 3, Page(s) S67–9

Abstract: ... 2 and ara-C 100 mg/m2 days 1-5 and 5 or 10 micrograms/m2 G-CSF. In case of insufficient yield ... chemotherapy was repeated using IL-3 and G-CSF for mobilization of stem cells. Fourteen patients received 18 ...

Abstract	Mobilization of Philadelphia chromosome (Ph) negative peripheral blood stem cells has been reported subsequent to intensive chemotherapy. We asked whether peripheral blood stem cells can be harvested subsequent to a less toxic chemotherapy regimen. Patients were treated with idarubicin 12 mg/m2 on day 1 and 2 and ara-C 100 mg/m2 days 1-5 and 5 or 10 micrograms/m2 G-CSF. In case of insufficient yield chemotherapy was repeated using IL-3 and G-CSF for mobilization of stem cells. Fourteen patients received 18 cycles of chemotherapy. The majority of patients were in late chronic phase and treated after secondary (interferon-alpha) IFN-alpha resistance. sufficient numbers of peripheral blood stem cells were harvested in 11 out of 14 patients. Although mixed Ph positive/Ph negative leukaphereses were harvested in the majority of patients, in no case were sufficient numbers of purely Ph negative progenitor cells for transplantation obtained. No toxic deaths were observed during the aplasia and the toxicity was acceptable. These preliminary results demonstrate that this procedure can be safely applied in patients with chronic phase CML and allows the harvesting of sufficient numbers of peripheral blood stem cells. The efficacy of this regimen for the mobilization of Ph negative cells should be further explored in patients at an earlier stage of the disease.
MeSH term(s)	Adult ; Antigens, CD34/metabolism ; Antineoplastic Combined Chemotherapy Protocols/administration & dosage ; Combined Modality Therapy ; Cytarabine/administration & dosage ; Drug Resistance ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Hematopoietic Stem Cell Transplantation/methods ; Hematopoietic Stem Cells/drug effects ; Hematopoietic Stem Cells/immunology ; Humans ; Idarubicin/administration & dosage ; Interferon-alpha/therapeutic use ; Interleukin-3/administration & dosage ; Leukapheresis ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/therapy ; Leukemia, Myeloid, Chronic-Phase/drug therapy ; Leukemia, Myeloid, Chronic-Phase/genetics ; Leukemia, Myeloid, Chronic-Phase/therapy ; Middle Aged ; Philadelphia Chromosome
Chemical Substances	Antigens, CD34 ; Interferon-alpha ; Interleukin-3 ; Cytarabine (04079A1RDZ) ; Granulocyte Colony-Stimulating Factor (143011-72-7) ; Idarubicin (ZRP63D75JW)
Language	English
Publishing date	1996-05
Publishing country	England
Document type	Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	632854-4
ISSN	1476-5365 ; 0268-3369 ; 0951-3078
ISSN (online)	1476-5365
ISSN	0268-3369 ; 0951-3078
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Article: Implementation of orbitrap mass spectrometry for improved GC-MS target analysis in lithium ion battery electrolytes.

Peschel, Christoph / Horsthemke, Fabian / Winter, Martin / Nowak, Sascha

MethodsX

2022 Volume 9, Page(s) 101621

Abstract: ... interferences of typical carbonate fragment ions e.g. caused by column bleeding were identified and false ...

Abstract	The implementation of orbitrap mass spectrometry for target analysis of volatile species in aged lithium-ion batteries was performed in a case study on butyl carbonates. In comparison to previously applied single quadrupole-based methods, major improvements were obtained.•Sensitivity was improved by effectively background free extracted ion chromatograms of identified marker fragment ions.•Typical isobaric interferences of typical carbonate fragment ions e.g. caused by column bleeding were identified and false positive identification avoided.•Analysis of isotope labeled electrolytes was optimized regarding mass spectrometric data reliability with mass accuracies <0.5 ppm and mass resolutions >100,000.
Language	English
Publishing date	2022-01-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2022.101621
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Article ; Online: A Review of Monitoring Techniques for Livestock Respiration and Sounds

Divya Handa / Joshua M. Peschel

Frontiers in Animal Science, Vol

2022 Volume 3

Abstract: ... respiration rate; contact technologies (e.g., accelerometers, pressure sensors, and thermistors) utilize sensors ... that are physically mounted on livestock while non-contact technologies (e.g., computer vision ...

Abstract	This article reviews the different techniques used to monitor the respiration and sounds of livestock. Livestock respiration is commonly assessed visually by observing abdomen fluctuation; however, the traditional methods are time consuming, subjective, being therefore impractical for large-scale operations and must rely on automation. Contact and non-contact technologies are used to automatically monitor respiration rate; contact technologies (e.g., accelerometers, pressure sensors, and thermistors) utilize sensors that are physically mounted on livestock while non-contact technologies (e.g., computer vision, thermography, and sound analysis) enable a non-invasive method of monitoring respiration. This work summarizes the advantages and disadvantages of contact and non-contact technologies and discusses the emerging role of non-contact sensors in automating monitoring for large-scale farming operations. This work is the first in-depth examination of automated monitoring technologies for livestock respiratory diseases; the findings and recommendations are important for livestock researchers and practitioners who can gain a better understanding of these different technologies, especially emerging non-contact sensing.
Keywords	respiration ; livestock ; sensors ; precision livestock farming ; animal production and health ; Veterinary medicine ; SF600-1100
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
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Article: Implementation of orbitrap mass spectrometry for improved GC-MS target analysis in lithium ion battery electrolytes

Peschel, Christoph / Horsthemke, Fabian / Winter, Martin / Nowak, Sascha

MethodsX. 2022, v. 9

2022

Abstract: ... interferences of typical carbonate fragment ions e.g. caused by column bleeding were identified and false ...

Abstract	The implementation of orbitrap mass spectrometry for target analysis of volatile species in aged lithium-ion batteries was performed in a case study on butyl carbonates. In comparison to previously applied single quadrupole-based methods, major improvements were obtained.•Sensitivity was improved by effectively background free extracted ion chromatograms of identified marker fragment ions.•Typical isobaric interferences of typical carbonate fragment ions e.g. caused by column bleeding were identified and false positive identification avoided.•Analysis of isotope labeled electrolytes was optimized regarding mass spectrometric data reliability with mass accuracies <0.5 ppm and mass resolutions >100,000.
Keywords	carbonates ; case studies ; isotopes ; lithium batteries
Language	English
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2830212-6
ISSN	2215-0161
ISSN	2215-0161
DOI	10.1016/j.mex.2022.101621
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