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Article ; Online: Workload Accomplished in Phase III Cardiac Rehabilitation.

Schultz, Katrina L / Foster, Carl / Radtke, Kimberley / Bramwell, Susan / Cortis, Cristina / Fusco, Andrea / Porcari, John P

Journal of functional morphology and kinesiology

2021 Volume 6, Issue 2

Abstract: Exercise training is an important component of clinical exercise programs. Although there are recognized guidelines for the amount of exercise to be accomplished (≥70,000 steps per week or ≥150 min per week at moderate intensity), there is virtually no ... ...

Abstract	Exercise training is an important component of clinical exercise programs. Although there are recognized guidelines for the amount of exercise to be accomplished (≥70,000 steps per week or ≥150 min per week at moderate intensity), there is virtually no documentation of how much exercise is actually accomplished in contemporary exercise programs. Having guidelines without evidence of whether they are being met is of limited value. We analyzed both the weekly step count and the session rating of perceived exertion (sRPE) of patients (
Language	English
Publishing date	2021-05-28
Publishing country	Switzerland
Document type	Journal Article
ISSN	2411-5142
ISSN (online)	2411-5142
DOI	10.3390/jfmk6020047
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Article ; Online: Author Correction: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP).

Jain, Sanjay / Pei, Liming / Spraggins, Jeffrey M / Angelo, Michael / Carson, James P / Gehlenborg, Nils / Ginty, Fiona / Gonçalves, Joana P / Hagood, James S / Hickey, John W / Kelleher, Neil L / Laurent, Louise C / Lin, Shin / Lin, Yiing / Liu, Huiping / Naba, Alexandra / Nakayasu, Ernesto S / Qian, Wei-Jun / Radtke, Andrea /

Robson, Paul / Stockwell, Brent R / Van de Plas, Raf / Vlachos, Ioannis S / Zhou, Mowei / Börner, Katy / Snyder, Michael P

Nature cell biology

2024

Language	English
Publishing date	2024-03-01
Publishing country	England
Document type	Published Erratum
ZDB-ID	1474722-4
ISSN	1476-4679 ; 1465-7392
ISSN (online)	1476-4679
ISSN	1465-7392
DOI	10.1038/s41556-024-01384-0
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Zs.A 5169: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Spatial Patterning Analysis of Cellular Ensembles (SPACE) enables statistically robust discovery of complex spatial organization at the cell and tissue level.

Schrom, Edward C / McCaffrey, Erin F / Radtke, Andrea J / Speranza, Emily / Arakkal, Leanne / Thakur, Nishant / Grant, Spencer / Germain, Ronald N

bioRxiv : the preprint server for biology

2023

Abstract: Spatial patterns of cells and other entities drive both physiologic and pathologic processes within tissues. While many imaging and transcriptomic methods document tissue organization, discerning these patterns is challenging, especially when they ... ...

Abstract	Spatial patterns of cells and other entities drive both physiologic and pathologic processes within tissues. While many imaging and transcriptomic methods document tissue organization, discerning these patterns is challenging, especially when they involve multiple entities in complex arrangements. To address this challenge, we present Spatial Patterning Analysis of Cellular Ensembles (SPACE), an R package for analysis of high-plex tissue images generated using any collection modality. Unlike existing platforms, SPACE detects context-dependent associations, quantitative gradients and orientations, and other organizational complexities. Via a robust information theoretic framework, SPACE explores all possible ensembles - single entities, pairs, triplets, and so on - and ranks the strongest patterns of tissue organization. Using lymph node images for which ground truth has been defined, we validate SPACE and demonstrate its advantages. We then use SPACE to reanalyze a public dataset of human tuberculosis granulomas, verifying known patterns and discovering new patterns with possible insights into disease progression.
Language	English
Publishing date	2023-12-10
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.12.08.570837
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Article: Mapping the lymphatic system across body scales and expertise domains: A report from the 2021 National Heart, Lung, and Blood Institute workshop at the Boston Lymphatic Symposium.

Singhal, Dhruv / Börner, Katy / Chaikof, Elliot L / Detmar, Michael / Hollmén, Maija / Iliff, Jeffrey J / Itkin, Maxim / Makinen, Taija / Oliver, Guillermo / Padera, Timothy P / Quardokus, Ellen M / Radtke, Andrea J / Suami, Hiroo / Weber, Griffin M / Rovira, Ilsa I / Muratoglu, Selen C / Galis, Zorina S

Frontiers in physiology

2023 Volume 14, Page(s) 1099403

Abstract: Enhancing our understanding of lymphatic anatomy from the microscopic to the anatomical scale is essential to discern how the structure and function of the lymphatic system interacts with different tissues and organs within the body and contributes to ... ...

Abstract	Enhancing our understanding of lymphatic anatomy from the microscopic to the anatomical scale is essential to discern how the structure and function of the lymphatic system interacts with different tissues and organs within the body and contributes to health and disease. The knowledge of molecular aspects of the lymphatic network is fundamental to understand the mechanisms of disease progression and prevention. Recent advances in mapping components of the lymphatic system using state of the art single cell technologies, the identification of novel biomarkers, new clinical imaging efforts, and computational tools which attempt to identify connections between these diverse technologies hold the potential to catalyze new strategies to address lymphatic diseases such as lymphedema and lipedema. This manuscript summarizes current knowledge of the lymphatic system and identifies prevailing challenges and opportunities to advance the field of lymphatic research as discussed by the experts in the workshop.
Language	English
Publishing date	2023-02-06
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2564217-0
ISSN	1664-042X
ISSN	1664-042X
DOI	10.3389/fphys.2023.1099403
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Article ; Online: Prediction of Exercise Capacity and Training Prescription from the 6-Minute Walk Test and Rating of Perceived Exertion.

Porcari, John P / Foster, Carl / Cress, Maria L / Larson, Rachel / Lewis, Hannah / Cortis, Cristina / Doberstein, Scott / Donahue, Marc / Fusco, Andrea / Radtke, Kimberly

Journal of functional morphology and kinesiology

2021 Volume 6, Issue 2

Abstract: Walking tests, such as the 6-min walk test (6MWT), are popular methods of estimating peak oxygen uptake ( ... ...

Abstract	Walking tests, such as the 6-min walk test (6MWT), are popular methods of estimating peak oxygen uptake (VO
Language	English
Publishing date	2021-06-14
Publishing country	Switzerland
Document type	Journal Article
ISSN	2411-5142
ISSN (online)	2411-5142
DOI	10.3390/jfmk6020052
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Article ; Online: Meeting report: 35th International Conference on Antiviral Research in Seattle, Washington, USA - March 21-25, 2022.

Spengler, Jessica R / Welch, Stephen R / Deval, Jerome / Gentry, Brian G / Brancale, Andrea / Carter, Kara / Moffat, Jennifer / Meier, Chris / Seley-Radtke, Katherine L / Schang, Luis M

Antiviral research

2022 Volume 211, Page(s) 105521

Abstract: The 35th International Conference on Antiviral Research (ICAR), sponsored by the International Society for Antiviral Research (ISAR), was held in Seattle, Washington, USA, on March 21-25, 2022 and concurrently through an interactive remote meeting ... ...

Abstract	The 35th International Conference on Antiviral Research (ICAR), sponsored by the International Society for Antiviral Research (ISAR), was held in Seattle, Washington, USA, on March 21-25, 2022 and concurrently through an interactive remote meeting platform. This report gives an overview of the conference on behalf of the society. It provides a general review of the meeting and awardees, summarizing the presentations and their main conclusions from the perspective of researchers active in many different areas of antiviral research and development. Through ICAR, leaders in the field of antiviral research were able to showcase their efforts, as participants learned about key advances in the field. The impact of these efforts was exemplified by many presentations on SARS-CoV-2 demonstrating the remarkable response to the ongoing pandemic, as well as future pandemic preparedness, by members of the antiviral research community. As we address ongoing outbreaks and seek to mitigate those in the future, this meeting continues to support outstanding opportunities for the exchange of knowledge and expertise while fostering cross-disciplinary collaborations in therapeutic and vaccine development. The 36th ICAR will be held in Lyon, France, March 13-17, 2023.
MeSH term(s)	Humans ; Antiviral Agents/therapeutic use ; Washington ; Iron-Dextran Complex ; COVID-19 ; SARS-CoV-2
Chemical Substances	Antiviral Agents ; Iron-Dextran Complex (9004-66-4)
Language	English
Publishing date	2022-12-31
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	306628-9
ISSN	1872-9096 ; 0166-3542
ISSN (online)	1872-9096
ISSN	0166-3542
DOI	10.1016/j.antiviral.2022.105521
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Zs.A 1627: Show issues

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Article ; Online: IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.

Radtke, Andrea J / Chu, Colin J / Yaniv, Ziv / Yao, Li / Marr, James / Beuschel, Rebecca T / Ichise, Hiroshi / Gola, Anita / Kabat, Juraj / Lowekamp, Bradley / Speranza, Emily / Croteau, Joshua / Thakur, Nishant / Jonigk, Danny / Davis, Jeremy L / Hernandez, Jonathan M / Germain, Ronald N

Nature protocols

2022 Volume 17, Issue 2, Page(s) 378–401

Abstract: High-content imaging is needed to catalog the variety of cellular phenotypes and multicellular ecosystems present in metazoan tissues. We recently developed iterative bleaching extends multiplexity (IBEX), an iterative immunolabeling and chemical ... ...

Abstract	High-content imaging is needed to catalog the variety of cellular phenotypes and multicellular ecosystems present in metazoan tissues. We recently developed iterative bleaching extends multiplexity (IBEX), an iterative immunolabeling and chemical bleaching method that enables multiplexed imaging (>65 parameters) in diverse tissues, including human organs relevant for international consortia efforts. IBEX is compatible with >250 commercially available antibodies and 16 unique fluorophores, and can be easily adopted to different imaging platforms using slides and nonproprietary imaging chambers. The overall protocol consists of iterative cycles of antibody labeling, imaging and chemical bleaching that can be completed at relatively low cost in 2-5 d by biologists with basic laboratory skills. To support widespread adoption, we provide extensive details on tissue processing, curated lists of validated antibodies and tissue-specific panels for multiplex imaging. Furthermore, instructions are included on how to automate the method using competitively priced instruments and reagents. Finally, we present a software solution for image alignment that can be executed by individuals without programming experience using open-source software and freeware. In summary, IBEX is a noncommercial method that can be readily implemented by academic laboratories and scaled to achieve high-content mapping of diverse tissues in support of a Human Reference Atlas or other such applications.
MeSH term(s)	Ecosystem
Language	English
Publishing date	2022-01-12
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, N.I.H., Intramural ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2244966-8
ISSN	1750-2799 ; 1754-2189
ISSN (online)	1750-2799
ISSN	1754-2189
DOI	10.1038/s41596-021-00644-9
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Article ; Online: Meeting report: 34th international conference on antiviral research.

Brancale, Andrea / Carter, Kara / Delang, Leen / Deval, Jerome / Durantel, David / Gentry, Brian G / Jordan, Robert / Julander, Justin G / Lo, Michael K / Pérez-Pérez, Maria-Jesús / Schang, Luis M / Seley-Radtke, Katherine L / Shi, Pei-Yong / Vasudevan, Subhash G / Whitley, Richard J / Spengler, Jessica R

Antiviral chemistry & chemotherapy

2022 Volume 30, Page(s) 20402066221130853

Abstract: As a result of the multiple gathering and travels restrictions during the SARS-CoV-2 pandemic, the annual meeting of the International Society for Antiviral Research (ISAR), the International Conference on Antiviral Research (ICAR), could not be held in ... ...

Abstract	As a result of the multiple gathering and travels restrictions during the SARS-CoV-2 pandemic, the annual meeting of the International Society for Antiviral Research (ISAR), the International Conference on Antiviral Research (ICAR), could not be held in person in 2021. Nonetheless, ISAR successfully organized a remote conference, retaining the most critical aspects of all ICARs, a collegiate gathering of researchers in academia, industry, government and non-governmental institutions working to develop, identify, and evaluate effective antiviral therapy for the benefit of all human beings. This article highlights the 2021 remote meeting, which presented the advances and objectives of antiviral and vaccine discovery, research, and development. The meeting resulted in a dynamic and effective exchange of ideas and information, positively impacting the prompt progress towards new and effective prophylaxis and therapeutics.
MeSH term(s)	Humans ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; SARS-CoV-2 ; COVID-19/drug therapy ; Pandemics
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2022-10-27
Publishing country	England
Document type	Journal Article
ZDB-ID	1033586-9
ISSN	2040-2066 ; 0956-3202
ISSN (online)	2040-2066
ISSN	0956-3202
DOI	10.1177/20402066221130853
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Zs.A 2950: Show issues

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Article: Applying Unsterile Microporous Tape onto Surgical Wounds: Tape Contamination and Clinical Rationale.

Ausen, Kjersti / Kroknes, Marthe L / Gunnarson, Gudjon / Radtke, Andreas

Plastic and reconstructive surgery. Global open

2021 Volume 9, Issue 6, Page(s) e3635

Abstract: Fomites are surfaces that carry contaminants and may cause infection. We wanted to assess the bacterial load on rolls of nonsterile microporous tape in a hospital setting and explore the scientific rationale behind the existing practice of applying ... ...

Abstract	Fomites are surfaces that carry contaminants and may cause infection. We wanted to assess the bacterial load on rolls of nonsterile microporous tape in a hospital setting and explore the scientific rationale behind the existing practice of applying unsterile adhesives onto a surgical wound. Methods: We analyzed the aerobic bacterial contamination in rolls of microporous tape collected from surgical theaters, outpatient clinics, and storage rooms at St. Olav's University Hospital, Trondheim, Norway between 2018 and 2020. We also reviewed the literature for relevant publications. Results: A total of 58 rolls were collected; 55 were included for final analysis. Exposed tape surfaces were significantly more contaminated than unexposed surfaces. Tape rolls from outpatient clinics were significantly more contaminated and contained a significantly greater variety of microbes than rolls from operation theaters and storage rooms. Unexposed surfaces from both operation theaters and storage rooms demonstrated very little contamination. Conclusions: Rolls of tape may act as fomites, but widespread use of adhesives is inevitable in hospital settings. Removing the outer layer of a tape roll before use may significantly reduce bacterial contamination. Given sufficient vigilance to avoid cross-contamination, inner layers of tape may represent a close-to-sterile alternative as surgical dressing. However, the economic savings constitute a negligible fraction of the total costs of the surgery, and the risk of contamination seems apparent. Scientific support of dressing a fresh surgical wound with unsterile microporous tape is lacking, and we therefore do not recommend the practice except in situations with very limited resources.
Language	English
Publishing date	2021-06-15
Publishing country	United States
Document type	Journal Article
ZDB-ID	2851682-5
ISSN	2169-7574 ; 2169-7574
ISSN (online)	2169-7574
ISSN	2169-7574
DOI	10.1097/GOX.0000000000003635
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Zs.A 7091: Show issues

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Article ; Online: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP).

Robson, Paul / Stockwell, Brent R / Van de Plas, Raf / Vlachos, Ioannis S / Zhou, Mowei / Börner, Katy / Snyder, Michael P

Nature cell biology

2023 Volume 25, Issue 8, Page(s) 1089–1100

Abstract: The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. As the HuBMAP moves past its ... ...

Abstract	The Human BioMolecular Atlas Program (HuBMAP) aims to create a multi-scale spatial atlas of the healthy human body at single-cell resolution by applying advanced technologies and disseminating resources to the community. As the HuBMAP moves past its first phase, creating ontologies, protocols and pipelines, this Perspective introduces the production phase: the generation of reference spatial maps of functional tissue units across many organs from diverse populations and the creation of mapping tools and infrastructure to advance biomedical research.
Language	English
Publishing date	2023-07-19
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1474722-4
ISSN	1476-4679 ; 1465-7392
ISSN (online)	1476-4679
ISSN	1465-7392
DOI	10.1038/s41556-023-01194-w
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Zs.A 5169: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
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