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  1. AU=Sasaki Kotaro
  2. AU=Abd-Elsayed Alaa
  3. AU="Jung, Hee-Jun"
  4. AU="Struckmann, Stephan"
  5. AU=Coward Richard
  6. AU="Ghazizadeh, Shabnam"
  7. AU="Rebecca A Butcher"
  8. AU="Kimberlyn Roosa"
  9. AU=Chian Ri-Cheng
  10. AU="Alzalzalah, Sayed"
  11. AU=Kaufman Jonathan J
  12. AU="Kim, Jin K"
  13. AU="Zevakov, S A"
  14. AU="Sui Phang"
  15. AU="Kolomeichuk, Lilia V"
  16. AU="Sabuj Kanti Mistry"
  17. AU="Basurto-Lozada, Daniela"
  18. AU="Takashima, Shin-Ichiro"
  19. AU="Teresinha Leal"
  20. AU="Angélique B van 't Wout"
  21. AU="Roberts, Nicholas J"
  22. AU="Chauhan, Gaurav B"
  23. AU=Hanjaya-Putra Donny
  24. AU=Powell James
  25. AU="Russell, Todd"
  26. AU=Forth Scott
  27. AU="Kreutzer, Susanne" AU="Kreutzer, Susanne"
  28. AU="St John, Maie"
  29. AU=Gerhardy A
  30. AU="Qi, Huixin"
  31. AU="Dobosiewicz, May"
  32. AU="Srivastava, Rakesh"
  33. AU="Grevtsov K.I."

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  1. Artikel ; Online: Developmental origins of mammalian spermatogonial stem cells: New perspectives on epigenetic regulation and sex chromosome function.

    Sasaki, Kotaro / Sangrithi, Mahesh

    Molecular and cellular endocrinology

    2023  Band 573, Seite(n) 111949

    Abstract: Male and female germ cells undergo genome-wide reprogramming during their development, and execute sex-specific programs to complete meiosis and successfully generate healthy gametes. While sexually dimorphic germ cell development is fundamental, ... ...

    Abstract Male and female germ cells undergo genome-wide reprogramming during their development, and execute sex-specific programs to complete meiosis and successfully generate healthy gametes. While sexually dimorphic germ cell development is fundamental, similarities and differences exist in the basic processes governing normal gametogenesis. At the simplest level, male gamete generation in mammals is centred on the activity of spermatogonial stem cells (SSCs), and an equivalent cell state is not present in females. Maintaining this unique SSC epigenetic state, while keeping to germ cell-intrinsic developmental programs, poses challenges for the correct completion of spermatogenesis. In this review, we highlight the origins of spermatogonia, comparing and contrasting them with female germline development to emphasize specific developmental processes that are required for their function as germline stem cells. We identify gaps in our current knowledge about human SSCs and further discuss the impact of the unique regulation of the sex chromosomes during spermatogenesis, and the roles of X-linked genes in SSCs.
    Mesh-Begriff(e) Animals ; Female ; Male ; Humans ; Epigenesis, Genetic ; Spermatogenesis/genetics ; Spermatogonia/physiology ; Cell Differentiation ; Sex Chromosomes/genetics ; Stem Cells/physiology ; Testis ; Mammals/genetics
    Sprache Englisch
    Erscheinungsdatum 2023-05-16
    Erscheinungsland Ireland
    Dokumenttyp Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 187438-x
    ISSN 1872-8057 ; 0303-7207
    ISSN (online) 1872-8057
    ISSN 0303-7207
    DOI 10.1016/j.mce.2023.111949
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: The developmental dynamics of the human male germline.

    Hargy, John / Sasaki, Kotaro

    Development (Cambridge, England)

    2023  Band 150, Heft 17

    Abstract: Male germ cells undergo a complex sequence of developmental events throughout fetal and postnatal life that culminate in the formation of haploid gametes: the spermatozoa. Errors in these processes result in infertility and congenital abnormalities in ... ...

    Abstract Male germ cells undergo a complex sequence of developmental events throughout fetal and postnatal life that culminate in the formation of haploid gametes: the spermatozoa. Errors in these processes result in infertility and congenital abnormalities in offspring. Male germ cell development starts when pluripotent cells undergo specification to sexually uncommitted primordial germ cells, which act as precursors of both oocytes and spermatozoa. Male-specific development subsequently occurs in the fetal testes, resulting in the formation of spermatogonial stem cells: the foundational stem cells responsible for lifelong generation of spermatozoa. Although deciphering such developmental processes is challenging in humans, recent studies using various models and single-cell sequencing approaches have shed new insight into human male germ cell development. Here, we provide an overview of cellular, signaling and epigenetic cascades of events accompanying male gametogenesis, highlighting conserved features and the differences between humans and other model organisms.
    Mesh-Begriff(e) Male ; Humans ; Germ Cells ; Spermatozoa ; Oocytes ; Adult Germline Stem Cells ; Cell Differentiation
    Sprache Englisch
    Erscheinungsdatum 2023-08-31
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.202046
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: An Exploratory Study on the Contractile Effects of Hydroxy-α-Sanshool and Hydroxy-β-Sanshool, the Active Ingredients of Daikenchuto, on the Internal Anal Sphincter.

    Maeda, Kotaro / Sasaki, Toshinobu

    Journal of the anus, rectum and colon

    2023  Band 7, Heft 3, Seite(n) 206–213

    Abstract: Objectives: Daikenchuto (DKT) enhances the contraction of the internal anal sphincter (IAS) in patients with constipation and fecal incontinence; however, the mechanism of its action is unknown. We investigated the effects of the active ingredients of ... ...

    Abstract Objectives: Daikenchuto (DKT) enhances the contraction of the internal anal sphincter (IAS) in patients with constipation and fecal incontinence; however, the mechanism of its action is unknown. We investigated the effects of the active ingredients of DKT (hydroxy-α-sanshool (HAS) and hydroxy-β-sanshool (HBS)) on the contractile activity of the canine rectum and IAS.
    Methods: Three male beagle dogs were prepared for each of the HAS, HBS, and control groups. Force transducers were attached to the rectal and IAS surfaces of the dogs, and the contractile responses were measured by telemetry under conscious conditions. HAS (10 mg/body) and HBS (2.5 mg/body) were administered intrarectally at doses previously identified from an effective dose of DKT extract (1.5 g/body), and contractile responses were recorded up to 6 h after administration. Contractile activity of the rectum and IAS was evaluated by observing the area under the curve (AUC) of the recorded contraction waveform. Plasma concentrations of HAS and HBS were measured before and after administration to confirm IAS exposure to both ingredients.
    Results: The mean AUC values of the IAS for the control, HAS, and HBS groups at 10 min after administration were 115, 87, and 220 (g-min), respectively, indicating a higher contraction in the HBS group, which was maintained for approximately 3 h. As for the rectum, no contractile response was observed in either the HAS or HBS groups. Plasma concentrations of both ingredients peaked at 20 min after administration.
    Conclusions: HBS could be involved in the contractile action of DKT on the IAS.
    Sprache Englisch
    Erscheinungsdatum 2023-07-25
    Erscheinungsland Japan
    Dokumenttyp Journal Article
    ISSN 2432-3853
    ISSN (online) 2432-3853
    DOI 10.23922/jarc.2023-001
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Advanced Pt-Based Core-Shell Electrocatalysts for Fuel Cell Cathodes.

    Zhao, Xueru / Sasaki, Kotaro

    Accounts of chemical research

    2022  Band 55, Heft 9, Seite(n) 1226–1236

    Abstract: ConspectusProton-exchange membrane fuel cells (PEMFCs) are highly efficient energy storage and conversion devices. Thus, the platinum group metal (PGM)-based catalysts which are the dominant choice for the PEMFCs have received extensive interest during ... ...

    Abstract ConspectusProton-exchange membrane fuel cells (PEMFCs) are highly efficient energy storage and conversion devices. Thus, the platinum group metal (PGM)-based catalysts which are the dominant choice for the PEMFCs have received extensive interest during the past couple of decades. However, the drawbacks in the existing PGM-based catalysts (i.e., high cost, slow kinetics, poor stability, etc.) still limit their applications in fuel cells. The Pt-based core-shell catalysts potentially alleviate these issues through the low Pt loading with the associated low cost and the high corrosion resistance and further improve the oxygen reduction reaction's (ORR's) activity and stability. This Account focuses on the synthetic strategies, catalytic mechanisms, factors influencing enhanced ORR performance, and applications in PEMFCs for the Pt-based core-shell catalysts. We first highlight the synthetic strategies for Pt-based core-shell catalysts including the galvanic displacement of an underpotentially deposited non-noble metal monolayer, thermal annealing, and dealloying methods, which can be scaled-up to meet the requirements of fuel cell operations. Subsequently, catalytic mechanisms such as the self-healing mechanism in the Pt monolayer on Pd core catalysts, the pinning effect of nitrogen (N) dopants in N-doped PtNi core-shell catalysts, and the ligand effect of the ordered intermetallic structure in L1
    Sprache Englisch
    Erscheinungsdatum 2022-04-22
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 1483291-4
    ISSN 1520-4898 ; 0001-4842
    ISSN (online) 1520-4898
    ISSN 0001-4842
    DOI 10.1021/acs.accounts.2c00057
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Reconstitution of Human Prospermatogonial Development from Human-Induced Pluripotent Stem Cells.

    Seita, Yasunari / Hwang, Young Sun / Sasaki, Kotaro

    Methods in molecular biology (Clifton, N.J.)

    2023  Band 2656, Seite(n) 145–159

    Abstract: There is a scarcity of information regarding the molecular mechanisms underlying human germ cell development due to limitations in obtaining the relevant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would ... ...

    Abstract There is a scarcity of information regarding the molecular mechanisms underlying human germ cell development due to limitations in obtaining the relevant materials. Reconstitution of human germ cell development from pluripotent stem cells in vitro would provide critical insight into the etiology of various reproductive conditions and disorders, including infertility.Recently, we reported the in vitro reconstitution of human prospermatogonial development from human-induced pluripotent stem cells through human primordial germ cell (PGC)-like cells (hPGCLCs) using long-term cultured xenogeneic reconstituted testes. Here, we describe a method to generate M-prospermatogonia-like cells (MLCs) and T1-prospermatogonia-like cells (T1LCs), which closely resemble M- and T1-prospermatogonia present in second-trimester human fetal testes in vivo.
    Mesh-Begriff(e) Male ; Humans ; Induced Pluripotent Stem Cells ; Germ Cells ; Pluripotent Stem Cells ; Cell Differentiation ; Testis
    Sprache Englisch
    Erscheinungsdatum 2023-05-30
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3139-3_8
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Single-nephron assessment of urate excretion in patients with IgA nephropathy.

    Marumoto, Hirokazu / Tsuboi, Nobuo / Sasaki, Takaya / Okabayashi, Yusuke / Haruhara, Kotaro / Kanzaki, Go / Ichida, Kimiyoshi / Yokoo, Takashi

    Clinical kidney journal

    2024  Band 17, Heft 3, Seite(n) sfae036

    Sprache Englisch
    Erscheinungsdatum 2024-02-12
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2655800-2
    ISSN 2048-8513 ; 2048-8505
    ISSN (online) 2048-8513
    ISSN 2048-8505
    DOI 10.1093/ckj/sfae036
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  7. Artikel ; Online: Erratum: High Albumin Clearance Predicts the Minimal Change Nephrotic Syndrome Relapse.

    Kuno, Hideaki / Kanzaki, Go / Sasaki, Takaya / Haruhara, Kotaro / Okabe, Masahiro / Yokote, Shinya / Koike, Kentaro / Nobuo, Tsuboi / Yokoo, Takashi

    Kidney360

    2024  

    Sprache Englisch
    Erscheinungsdatum 2024-04-25
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2641-7650
    ISSN (online) 2641-7650
    DOI 10.34067/KID.0000000000000451
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  8. Artikel ; Online: Dairy intake and the risk of incidental hypertension.

    Tsuboi, Nobuo / Sasaki, Takaya / Haruhara, Kotaro

    Hypertension research : official journal of the Japanese Society of Hypertension

    2022  Band 45, Heft 9, Seite(n) 1511–1513

    Mesh-Begriff(e) Dairy Products/adverse effects ; Earthquakes ; Humans ; Hypertension/physiopathology ; Incidence ; Japan ; Survivors
    Sprache Englisch
    Erscheinungsdatum 2022-06-22
    Erscheinungsland England
    Dokumenttyp Editorial ; Comment
    ZDB-ID 1175297-x
    ISSN 1348-4214 ; 0916-9636
    ISSN (online) 1348-4214
    ISSN 0916-9636
    DOI 10.1038/s41440-022-00966-5
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  9. Artikel ; Online: Longitudinal changes in depressive symptoms associated with social isolation after the Great East Japan Earthquake in Iwate Prefecture: findings from the TMM CommCohort study.

    Kotozaki, Yuka / Tanno, Kozo / Otsuka, Kotaro / Sasaki, Ryohei / Sasaki, Makoto

    BMC public health

    2023  Band 23, Heft 1, Seite(n) 1154

    Abstract: Background: Whether past disaster experiences affect the association between changes in social isolation and depressive symptoms is largely unknown. This study examined the association between changes in social isolation and depressive symptoms among ... ...

    Abstract Background: Whether past disaster experiences affect the association between changes in social isolation and depressive symptoms is largely unknown. This study examined the association between changes in social isolation and depressive symptoms among survivors who experienced earthquake damage in the aftermath of the Great East Japan Earthquake (GEJE).
    Methods: We analyzed longitudinal data from 10,314 participants who responded to self-report questionnaires on the Lubben Social Network Scale-6 (LSNS-6) and the Center for Epidemiological Studies-Depressive Scale (CES-D) in both the baseline survey (FY2013 to FY2015) and follow-up survey (FY2017 to FY2019) after the GEJE. According to changes in the presence of social isolation (< 12 of LSNS-6) at two time points, participants were categorized into four groups: "not socially isolated," "improved socially isolated," "newly socially isolated," and "continuously socially isolated." At the follow-up survey, a CES-D score of ≥ 16 indicates the presence of depressive symptoms. The adjusted odds ratios (AORs) and 95% confidence intervals (CIs) were estimated using the logistic regression analysis to examine the influence of the change in social isolation over four years on depressive symptoms.
    Results: Participants who were newly socially isolated had a significantly higher prevalence of depressive symptoms than those who were not socially isolated (AOR = 1.89, 95% CI = 1.61 - 2.23). In addition, AORs were highest for those who were continuously socially isolated and had experienced house damage (AOR = 2.17, 95% CI = 1.73 - 2.72) and those who were newly socially isolated and had not experienced the death of family members due to the GEJE (AOR = 1.88, 95%CI = 1.60 - 2.22).
    Conclusion: Our longitudinal findings suggest that being newly or continuously socially isolated is associated with a risk of depressive symptoms, not only among those who had experienced house damage or the death of a family member, but also those who had not, in the disaster-affected area. Our study underlines the clinical importance of social isolation after a large-scale natural disaster and draws attention to the need for appropriate prevention measures.
    Mesh-Begriff(e) Humans ; Earthquakes ; Japan/epidemiology ; Depression/epidemiology ; Disasters ; Social Isolation
    Sprache Englisch
    Erscheinungsdatum 2023-06-20
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2041338-5
    ISSN 1471-2458 ; 1471-2458
    ISSN (online) 1471-2458
    ISSN 1471-2458
    DOI 10.1186/s12889-023-16082-z
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Loneliness without distress, chosen isolation (solitude) at the workplace, and mental health and job performance: a cross-sectional study of Japanese employees.

    Sasaki, Natsu / Kuroda, Reiko / Tsuno, Kanami / Imamura, Kotaro / Eguchi, Hisashi / Shimazu, Akihito / Kawakami, Norito

    Industrial health

    2024  

    Abstract: While loneliness and social isolation in the workplace affect the mental health and job performance of employees, the effects of loneliness without distress and solitude (i.e., chosen isolation) on these outcomes are unclear. The cross-sectional ... ...

    Abstract While loneliness and social isolation in the workplace affect the mental health and job performance of employees, the effects of loneliness without distress and solitude (i.e., chosen isolation) on these outcomes are unclear. The cross-sectional association was examined by using online survey of full-time employees in Japan (n=846). The results showed that the "loneliness with distress" group had significantly higher psychological distress and lower job performance than the other groups. Work engagement was lower both in the "loneliness with distress" and "loneliness without distress" groups, compared to the "non-loneliness" group. The "unchosen isolation" and the "solitude" groups had poorer scores of psychological distress, work engagement, and work performance, compared to the "non-isolation" group. The preliminary findings showed that loneliness without distress and solitude were associated with poor levels of mental health and job performance and should become a target of mental health promotion interventions in the workplace.
    Sprache Englisch
    Erscheinungsdatum 2024-04-08
    Erscheinungsland Japan
    Dokumenttyp Journal Article
    ZDB-ID 214185-1
    ISSN 1880-8026 ; 0019-8366
    ISSN (online) 1880-8026
    ISSN 0019-8366
    DOI 10.2486/indhealth.2023-0181
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