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Book ; Online ; Thesis: Die Rolle des olfaktorischen Epithels in der initialen Phase der Infektion mit dem neurotropen Borna disease virus

Kupke, Alexandra [Verfasser]

2016

Author's details	Alexandra Kupke
Keywords	Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
Subject code	sg630
Language	German
Publisher	Universitätsbibliothek
Publishing place	Gießen
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

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Article ; Online: Short- and Long-Interval Prime-Boost Vaccination with the Candidate Vaccines MVA-SARS-2-ST and MVA-SARS-2-S Induces Comparable Humoral and Cell-Mediated Immunity in Mice.

Kalodimou, Georgia / Jany, Sylvia / Freudenstein, Astrid / Schwarz, Jan Hendrik / Limpinsel, Leonard / Rohde, Cornelius / Kupke, Alexandra / Becker, Stephan / Volz, Asisa / Tscherne, Alina / Sutter, Gerd

Viruses

2023 Volume 15, Issue 5

Abstract: The COVID-19 pandemic caused significant human health and economic consequences. Due to the ability of SARS-CoV-2 to spread rapidly and to cause severe disease and mortality in certain population groups, vaccines are essential for controlling the ... ...

Abstract	The COVID-19 pandemic caused significant human health and economic consequences. Due to the ability of SARS-CoV-2 to spread rapidly and to cause severe disease and mortality in certain population groups, vaccines are essential for controlling the pandemic in the future. Several licensed vaccines have shown improved protection against SARS-CoV-2 after extended-interval prime-boost immunizations in humans. Therefore, in this study, we aimed to compare the immunogenicity of our two Modified Vaccinia virus Ankara (MVA) based COVID-19 candidate vaccines MVA-SARS-2-S and MVA-SARS-2-ST after short- and long-interval prime-boost immunization schedules in mice. We immunized BALB/c mice using 21-day (short-interval) or 56-day (long-interval) prime-boost vaccination protocols and analyzed spike (S)-specific CD8 T cell immunity and humoral immunity. The two schedules induced robust CD8 T cell responses with no significant differences in their magnitude. Furthermore, both candidate vaccines induced comparable levels of total S, and S2-specific IgG binding antibodies. However, MVA-SARS-2-ST consistently elicited higher amounts of S1-, S receptor binding domain (RBD), and SARS-CoV-2 neutralizing antibodies in both vaccination protocols. Overall, we found very comparable immune responses following short- or long-interval immunization. Thus, our results suggest that the chosen time intervals may not be suitable to observe potential differences in antigen-specific immunity when testing different prime-boost intervals with our candidate vaccines in the mouse model. Despite this, our data clearly showed that MVA-SARS-2-ST induced superior humoral immune responses relative to MVA-SARS-2-S after both immunization schedules.
MeSH term(s)	Humans ; Animals ; Mice ; SARS-CoV-2 ; Pandemics ; COVID-19/prevention & control ; Vaccinia virus ; Vaccination/methods ; Antibodies, Viral ; Immunity, Cellular ; Immunity, Humoral
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2023-05-17
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15051180
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: T-bet

Rauch, Eileen / Amendt, Timm / Lopez Krol, Aleksandra / Lang, Fabian B / Linse, Vincent / Hohmann, Michelle / Keim, Ann-Christin / Kreutzer, Susanne / Kawengian, Kevin / Buchholz, Malte / Duschner, Philipp / Grauer, Saskia / Schnierle, Barbara / Ruhl, Andreas / Burtscher, Ingo / Dehnert, Sonja / Kuria, Chege / Kupke, Alexandra / Paul, Stephanie /

Liehr, Thomas / Lechner, Marcus / Schnare, Markus / Kaufmann, Andreas / Huber, Magdalena / Winkler, Thomas H / Bauer, Stefan / Yu, Philipp

Nature communications

2024 Volume 15, Issue 1, Page(s) 1229

Abstract: Endogenous retroviruses (ERVs) are an integral part of the mammalian genome. The role of immune control of ERVs in general is poorly defined as is their function as anti-cancer immune targets or drivers of autoimmune disease. Here, we generate mouse- ... ...

Abstract	Endogenous retroviruses (ERVs) are an integral part of the mammalian genome. The role of immune control of ERVs in general is poorly defined as is their function as anti-cancer immune targets or drivers of autoimmune disease. Here, we generate mouse-strains where Moloney-Murine Leukemia Virus tagged with GFP (ERV-GFP) infected the mouse germline. This enables us to analyze the role of genetic, epigenetic and cell intrinsic restriction factors in ERV activation and control. We identify an autoreactive B cell response against the neo-self/ERV antigen GFP as a key mechanism of ERV control. Hallmarks of this response are spontaneous ERV-GFP
MeSH term(s)	Animals ; Mice ; Autoimmune Diseases/genetics ; B-Lymphocytes/immunology ; Endogenous Retroviruses/genetics ; Mammals/genetics
Language	English
Publishing date	2024-02-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-45201-6
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Article ; Online: Osteogenesis imperfecta in a male holstein calf associated with a possible oligogenic origin.

Zhang, Xuying / Hirschfeld, Marc / Beck, Julia / Kupke, Alexandra / Köhler, Kernt / Schütz, Ekkehard / Brenig, Bertram

The veterinary quarterly

2019 Volume 40, Issue 1, Page(s) 58–67

Abstract: Background: ...

Abstract	Background:
MeSH term(s)	Animals ; Autopsy ; Case-Control Studies ; Cattle ; Cattle Diseases/genetics ; Cattle Diseases/pathology ; Genetic Predisposition to Disease/genetics ; Genotype ; Male ; Multifactorial Inheritance ; Mutation/genetics ; Osteogenesis Imperfecta/genetics ; Osteogenesis Imperfecta/pathology ; Osteogenesis Imperfecta/veterinary
Language	English
Publishing date	2019-12-20
Publishing country	England
Document type	Journal Article
ZDB-ID	754161-2
ISSN	1875-5941 ; 0165-2176
ISSN (online)	1875-5941
ISSN	0165-2176
DOI	10.1080/01652176.2020.1721611
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Article ; Online: Cyclosporin A Reveals Potent Antiviral Effects in Preclinical Models of SARS-CoV-2 Infection.

Sauerhering, Lucie / Kuznetsova, Irina / Kupke, Alexandra / Meier, Lars / Halwe, Sandro / Rohde, Cornelius / Schmidt, Jörg / Morty, Rory E / Danov, Olga / Braun, Armin / Vadász, István / Becker, Stephan / Herold, Susanne

American journal of respiratory and critical care medicine

2022 Volume 205, Issue 8, Page(s) 964–968

MeSH term(s)	Antiviral Agents/therapeutic use ; COVID-19/drug therapy ; Cyclosporine/pharmacology ; Cyclosporine/therapeutic use ; Drug Evaluation, Preclinical ; Humans ; SARS-CoV-2
Chemical Substances	Antiviral Agents ; Cyclosporine (83HN0GTJ6D)
Language	English
Publishing date	2022-04-10
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1180953-x
ISSN	1535-4970 ; 0003-0805 ; 1073-449X
ISSN (online)	1535-4970
ISSN	0003-0805 ; 1073-449X
DOI	10.1164/rccm.202108-1830LE
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Article: Intranasal Location and Immunohistochemical Characterization of the Equine Olfactory Epithelium.

Kupke, Alexandra / Wenisch, Sabine / Failing, Klaus / Herden, Christiane

Frontiers in neuroanatomy

2016 Volume 10, Page(s) 97

Abstract: The olfactory epithelium (OE) is the only body site where neurons contact directly the environment and are therefore exposed to a broad variation of substances and insults. It can serve as portal of entry for neurotropic viruses which spread via the ... ...

Abstract	The olfactory epithelium (OE) is the only body site where neurons contact directly the environment and are therefore exposed to a broad variation of substances and insults. It can serve as portal of entry for neurotropic viruses which spread via the olfactory pathway to the central nervous system. For horses, it has been proposed and concluded mainly from rodent studies that different viruses, e.g., Borna disease virus, equine herpesvirus 1 (EHV-1), hendra virus, influenza virus, rabies virus, vesicular stomatitis virus can use this route. However, little is yet known about cytoarchitecture, protein expression and the intranasal location of the equine OE. Revealing differences in cytoarchitecture or protein expression pattern in comparison to rodents, canines, or humans might help to explain varying susceptibility to certain intranasal virus infections. On the other hand, disclosing similarities especially between rodents and other species, e.g., horses would help to underscore transferability of rodent models. Analysis of the complete noses of five adult horses revealed that in the equine OE two epithelial subtypes with distinct marker expression exist, designated as
Language	English
Publishing date	2016-10-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2452969-2
ISSN	1662-5129
ISSN	1662-5129
DOI	10.3389/fnana.2016.00097
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Article ; Online: Intranasal Borna Disease Virus (BoDV-1) Infection: Insights into Initial Steps and Potential Contagiosity.

Kupke, Alexandra / Becker, Sabrina / Wewetzer, Konstantin / Ahlemeyer, Barbara / Eickmann, Markus / Herden, Christiane

International journal of molecular sciences

2019 Volume 20, Issue 6

Abstract: Mammalian Bornavirus (BoDV-1) typically causes a fatal neurologic disorder in horses and sheep, and was recently shown to cause fatal encephalitis in humans with and without transplant reception. It has been suggested that BoDV-1 enters the central ... ...

Abstract	Mammalian Bornavirus (BoDV-1) typically causes a fatal neurologic disorder in horses and sheep, and was recently shown to cause fatal encephalitis in humans with and without transplant reception. It has been suggested that BoDV-1 enters the central nervous system (CNS) via the olfactory pathway. However, (I) susceptible cell types that replicate the virus for successful spread, and (II) the role of olfactory ensheathing cells (OECs), remained unclear. To address this, we studied the intranasal infection of adult rats with BoDV-1 in vivo and in vitro, using olfactory mucosal (OM) cell cultures and the cultures of purified OECs. Strikingly, in vitro and in vivo, viral antigen and mRNA were present from four days post infection (dpi) onwards in the olfactory receptor neurons (ORNs), but also in all other cell types of the OM, and constantly in the OECs. In contrast, in vivo, BoDV-1 genomic RNA was only detectable in adult and juvenile ORNs, nerve fibers, and in OECs from 7 dpi on. In vitro, the rate of infection of OECs was significantly higher than that of the OM cells, pointing to a crucial role of OECs for infection via the olfactory pathway. Thus, this study provides important insights into the transmission of neurotropic viral infections with a zoonotic potential.
MeSH term(s)	Animals ; Borna Disease/virology ; Borna disease virus/genetics ; Borna disease virus/pathogenicity ; Cell Culture Techniques ; Cells, Cultured ; Disease Models, Animal ; Humans ; Olfactory Bulb/cytology ; Olfactory Bulb/virology ; Olfactory Mucosa/cytology ; Olfactory Mucosa/virology ; RNA, Viral/genetics ; Rats ; Zoonoses/virology
Chemical Substances	RNA, Viral
Language	English
Publishing date	2019-03-15
Publishing country	Switzerland
Document type	Comparative Study ; Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms20061318
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Article: Protective CD8+ T Cell Response Induced by Modified Vaccinia Virus Ankara Delivering Ebola Virus Nucleoprotein.

Kupke, Alexandra / Volz, Asisa / Dietzel, Erik / Freudenstein, Astrid / Schmidt, Jörg / Shams-Eldin, Hosam / Jany, Sylvia / Sauerhering, Lucie / Krähling, Verena / Gellhorn Serra, Michelle / Herden, Christiane / Eickmann, Markus / Becker, Stephan / Sutter, Gerd

Vaccines

2022 Volume 10, Issue 4

Abstract: The urgent need for vaccines against Ebola virus (EBOV) was underscored by the large outbreak in West Africa (2014-2016). Since then, several promising vaccine candidates have been tested in pre-clinical and clinical studies. As a result, two vaccines ... ...

Abstract	The urgent need for vaccines against Ebola virus (EBOV) was underscored by the large outbreak in West Africa (2014-2016). Since then, several promising vaccine candidates have been tested in pre-clinical and clinical studies. As a result, two vaccines were approved for human use in 2019/2020, of which one includes a heterologous adenovirus/Modified Vaccinia virus Ankara (MVA) prime-boost regimen. Here, we tested new vaccine candidates based on the recombinant MVA vector, encoding the EBOV nucleoprotein (MVA-EBOV-NP) or glycoprotein (MVA-EBOV-GP) for their efficacy after homologous prime-boost immunization in mice. Our aim was to investigate the role of each antigen in terms of efficacy and correlates of protection. Sera of mice vaccinated with MVA-EBOV-GP were virus-neutralizing and MVA-EBOV-NP immunization readily elicited interferon-γ-producing NP-specific CD8+ T cells. While mock-vaccinated mice succumbed to EBOV infection, all vaccinated mice survived and showed drastically decreased viral loads in sera and organs. In addition, MVA-EBOV-NP vaccinated mice became susceptible to lethal EBOV infection after depletion of CD8+ T cells prior to challenge. This study highlights the potential of MVA-based vaccines to elicit humoral immune responses as well as a strong and protective CD8+ T cell response and contributes to understanding the possible underlying mechanisms.
Language	English
Publishing date	2022-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10040533
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Article ; Online: Osteogenesis imperfecta in a male holstein calf associated with a possible oligogenic origin

Xuying Zhang / Marc Hirschfeld / Julia Beck / Alexandra Kupke / Kernt Köhler / Ekkehard Schütz / Bertram Brenig

Veterinary Quarterly, Vol 40, Iss 1, Pp 58-

2020 Volume 67

Abstract: Background Neuromusculoskeletal anomalies generally in combination with severe clinical symptoms, comprise a heterogeneous group of fairly common and mostly fatal disorders in man and animals. Osteogenesis imperfecta (OI), also known as brittle bone ... ...

Abstract	Background Neuromusculoskeletal anomalies generally in combination with severe clinical symptoms, comprise a heterogeneous group of fairly common and mostly fatal disorders in man and animals. Osteogenesis imperfecta (OI), also known as brittle bone disease, causes bone fragility and deformity. Prominent extra-skeletal accessory manifestations of OI comprise blue/gray sclerae, hearing impairment, lung abnormalities and hypercalciuria. Cases of OI in cattle have been reported. However, no causative mutations have been identified in cattle so far. Aim To report a possible oligogenic origin identified in a calf from clinically healthy parents suffering from OI. Materials and Methods A neonatal embryo transfer male Holstein calf developing multiple fractures with bone tissue showing marked osteopenia was used for whole genome re-sequencing as well as its parents. In addition, 2,612 randomly chosen healthy Holstein cattle were genotyped as well as controls. Results Sixteen candidate genes with potential protein-altering variants were selected revealing non-synonymous variants only within IFITM5 and CRTAP genes. However, in-depth gene analysis did not result in the identification of a single causative mutation in the OI calf. Conclusion The analysis of the OI case revealed a possible oligogenic origin of the disease attributable to additive effects of three candidate genes, i.e., ABCA13, QRFPR, and IFTIM5. Clinical relevance Most OI cases in humans and domestic animals reported so far are caused by distinct dominant or recessive monogenic mutations, therefore a potential oligogenic additive genetic effect is a novel finding. Furthermore, the case presented here demonstrates that cross-species genetic analyses might not always be straightforward.
Keywords	bovine ; cattle ; cow ; osteodysplasia ; osteodystrophy ; osteogenesis imperfecta ; brittle bone disease ; congenital bone disorder ; embryo transfer ; malformation ; Veterinary medicine ; SF600-1100
Subject code	616
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	Taylor & Francis Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Learning Weighted Automata over Principal Ideal Domains

van Heerdt, Gerco / Kupke, Clemens / Rot, Jurriaan / Silva, Alexandra

2019

Abstract: In this paper, we study active learning algorithms for weighted automata over a semiring. We show that a variant of Angluin's seminal \LStar\ algorithm works when the semiring is a principal ideal domain, but not for general semirings such as the natural ...

Abstract	In this paper, we study active learning algorithms for weighted automata over a semiring. We show that a variant of Angluin's seminal \LStar\ algorithm works when the semiring is a principal ideal domain, but not for general semirings such as the natural numbers.
Keywords	Computer Science - Formal Languages and Automata Theory
Publishing date	2019-11-11
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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