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  1. Article ; Online: Fungal Infection during COVID-19: Does

    Fekkar, Arnaud / Poignon, Corentin / Blaize, Marion / Lampros, Alexandre

    American journal of respiratory and critical care medicine

    2020  Volume 202, Issue 6, Page(s) 902–903

    MeSH term(s) Aspergillosis ; Aspergillus ; Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Humans ; Pandemics ; Pneumonia, Viral ; Pulmonary Aspergillosis ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-08-17
    Publishing country United States
    Document type Letter ; Comment
    ZDB-ID 1180953-x
    ISSN 1535-4970 ; 0003-0805 ; 1073-449X
    ISSN (online) 1535-4970
    ISSN 0003-0805 ; 1073-449X
    DOI 10.1164/rccm.202005-1945LE
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Invasive pulmonary fusariosis in an immunocompetent critically ill patient with severe COVID-19.

    Poignon, C / Blaize, M / Vezinet, C / Lampros, A / Monsel, A / Fekkar, A

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2020  Volume 26, Issue 11, Page(s) 1582–1584

    MeSH term(s) Antifungal Agents/therapeutic use ; Betacoronavirus/isolation & purification ; COVID-19 ; Coronavirus Infections/complications ; Coronavirus Infections/diagnosis ; Coronavirus Infections/therapy ; Critical Illness ; Fusariosis/complications ; Fusariosis/diagnosis ; Fusariosis/therapy ; Fusarium/drug effects ; Fusarium/isolation & purification ; Humans ; Immunocompetence ; Intubation, Intratracheal ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/complications ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/therapy ; SARS-CoV-2 ; Treatment Outcome
    Chemical Substances Antifungal Agents
    Keywords covid19
    Language English
    Publishing date 2020-06-30
    Publishing country England
    Document type Case Reports ; Letter
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2020.06.026
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Fungal Infection during COVID-19: Does Aspergillus Mean Secondary Invasive Aspergillosis?

    Fekkar, Arnaud / Poignon, Corentin / Blaize, Marion / Lampros, Alexandre

    Am J Respir Crit Care Med

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #656344
    Database COVID19

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  4. Article ; Online: Rapid selection of a cefiderocol-resistant Escherichia coli producing NDM-5 associated with a single amino acid substitution in the CirA siderophore receptor.

    Jousset, Agnès B / Poignon, Corentin / Yilmaz, Seher / Bleibtreu, Alexandre / Emeraud, Cécile / Girlich, Delphine / Naas, Thierry / Robert, Jérôme / Bonnin, Rémy A / Dortet, Laurent

    The Journal of antimicrobial chemotherapy

    2023  Volume 78, Issue 4, Page(s) 1125–1127

    MeSH term(s) Escherichia coli/genetics ; Escherichia coli/metabolism ; Siderophores ; Amino Acid Substitution ; Cephalosporins/pharmacology ; Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests ; beta-Lactamases/metabolism ; Drug Resistance, Multiple, Bacterial/genetics ; Cefiderocol
    Chemical Substances Siderophores ; Cephalosporins ; Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
    Language English
    Publishing date 2023-02-28
    Publishing country England
    Document type Letter
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkad004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Assessment of Droplet Digital PCR for the Detection and Absolute Quantification of Toxoplasma gondii: A Comparative Retrospective Study.

    Nabet, Cécile / Brossas, Jean-Yves / Poignon, Corentin / Bouzidi, Amira / Paris, Luc / Touafek, Feriel / Varlet-Marie, Emmanuelle / Sterkers, Yvon / Passebosc-Faure, Karine / Dardé, Marie-Laure / Piarroux, Renaud / Denis, Jérôme Alexandre

    The Journal of molecular diagnostics : JMD

    2023  Volume 25, Issue 7, Page(s) 467–476

    Abstract: Accurate tools for Toxoplasma gondii detection and quantification can be valuable for the early and effective management of toxoplasmosis. Droplet digital PCR (ddPCR) is a next-generation end-point PCR technique with high performance. The objective of ... ...

    Abstract Accurate tools for Toxoplasma gondii detection and quantification can be valuable for the early and effective management of toxoplasmosis. Droplet digital PCR (ddPCR) is a next-generation end-point PCR technique with high performance. The objective of the study was to evaluate the performance of ddPCR for the detection and absolute quantification of T. gondii. From January 2019 to October 2020, DNA samples collected at the Laboratory of Parasitology and Mycology of Pitié-Salpêtrière Hospital in Paris were retrospectively analyzed by ddPCR and real-time quantitative PCR (qPCR). To detect T. gondii with the best sensitivity possible, the REP-529 multicopy target was used. For absolute quantification of T. gondii, a specific single-copy target of α-tubulin was designed. T. gondii detection by ddPCR and qPCR was strongly correlated (R
    MeSH term(s) Humans ; Retrospective Studies ; Toxoplasma/genetics ; Tubulin/genetics ; Sensitivity and Specificity ; Real-Time Polymerase Chain Reaction/methods
    Chemical Substances Tubulin
    Language English
    Publishing date 2023-04-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2000060-1
    ISSN 1943-7811 ; 1525-1578
    ISSN (online) 1943-7811
    ISSN 1525-1578
    DOI 10.1016/j.jmoldx.2023.03.006
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Invasive pulmonary fusariosis in an immunocompetent critically ill patient with severe COVID-19

    Poignon, C / Blaize, M / Vezinet, C / Lampros, A / Monsel, A / Fekkar, A

    Clin Microbiol Infect

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #625559
    Database COVID19

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  7. Article ; Online: Invasive pulmonary fusariosis in an immunocompetent critically ill patient with severe COVID-19

    Poignon, C. / Blaize, M. / Vezinet, C. / Lampros, A. / Monsel, A. / Fekkar, A.

    Clinical Microbiology and Infection

    2020  Volume 26, Issue 11, Page(s) 1582–1584

    Keywords Microbiology (medical) ; Infectious Diseases ; General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2020.06.026
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article: SARS-CoV-2 Neutralizing Responses in Various Populations, at the Time of SARS-CoV-2 Variant Virus Emergence: Evaluation of Two Surrogate Neutralization Assays in Front of Whole Virus Neutralization Test.

    Marot, Stephane / Bocar Fofana, Djeneba / Flandre, Philippe / Malet, Isabelle / Zafilaza, Karen / Leducq, Valentin / Vivien, Diane / Mrabet, Sarah / Poignon, Corentin / Calvez, Vincent / Morand-Joubert, Laurence / Marcelin, Anne-Geneviève / Gozlan, Joel

    Life (Basel, Switzerland)

    2022  Volume 12, Issue 12

    Abstract: The SARS-CoV-2 neutralizing antibodies response is the best indicator of effective protection after infection and/or vaccination, but its evaluation requires tedious cell-based experiments using an infectious virus. We analyzed, in 105 patients with ... ...

    Abstract The SARS-CoV-2 neutralizing antibodies response is the best indicator of effective protection after infection and/or vaccination, but its evaluation requires tedious cell-based experiments using an infectious virus. We analyzed, in 105 patients with various histories of SARS-CoV-2 infection and/or vaccination, the neutralizing response using a virus neutralization test (VNT) against B.1, Alpha, Beta and Omicron variants, and compared the results with two surrogate assays based on antibody-mediated blockage of the ACE2-RBD interaction (Lateral Flow Boditech and ELISA Genscript). The strongest response was observed for recovered COVID-19 patients receiving one vaccine dose. Naïve patients receiving 2 doses of mRNA vaccine also demonstrate high neutralization titers against B.1, Alpha and Beta variants, but only 34.3% displayed a neutralization activity against the Omicron variant. On the other hand, non-infected patients with half vaccination schedules displayed a weak and inconstant activity against all isolates. Non-vaccinated COVID-19 patients kept a neutralizing activity against B.1 and Alpha up to 12 months after recovery but a decreased activity against Beta and Omicron. Both surrogate assays displayed a good correlation with the VNT. However, an adaptation of the cut-off positivity was necessary, especially for the most resistant Beta and Omicron variants. We validated two simple and reliable surrogate neutralization assays, which may favorably replace cell-based methods, allowing functional analysis on a larger scale.
    Language English
    Publishing date 2022-12-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662250-6
    ISSN 2075-1729
    ISSN 2075-1729
    DOI 10.3390/life12122064
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  9. Article ; Online: Électrophorèse des lipoprotéines sériques (lipoprotéinogramme) par le kit Hydragel Lipo + Lp(a)® (Sebia) : évaluation de la coloration au Fat Red 7B.

    Bittar, Randa / Pierrat, Gautier / Koujah, Nabil / Poignon, Corentin / Cherfils, Corinne / Fesel-Fouquier, Valérie / Barbot-Trystram, Laurence / Bonnefont-Rousselot, Dominique

    Annales de biologie clinique

    2020  Volume 78, Issue 1, Page(s) 17–26

    Abstract: The lipoproteinogram (or lipidogram) consists in an electrophoretic separation of the main classes of serum lipoproteins. Separation was done in agarose gel using the Sebia Hydragel Lipo + Lp(a)® kit. A repeatability study (n=6) was conducted on 3 sera ( ... ...

    Title translation Electrophoresis of serum lipoproteins (lipoproteinogram) with the Hydragel Lipo + Lp(a)® (Sebia) kit: evaluation of Fat Red 7B staining.
    Abstract The lipoproteinogram (or lipidogram) consists in an electrophoretic separation of the main classes of serum lipoproteins. Separation was done in agarose gel using the Sebia Hydragel Lipo + Lp(a)® kit. A repeatability study (n=6) was conducted on 3 sera (1 normolipidemic, 1 hypertriglyceridemic and 1 with a high Lp(a) concentration). The reproducibility was studied on these 3 sera and on an ascites liquid containing chylomicrons, upon 6 days (n=6). A quantitative approach was made by studying areas under the curve and percentages of fractions. In both cases (repeatability and reproducibility), the revelation of the lipoproteins in the gel after electrophoretic migration was made either by staining with Sudan Black (procedure recommended by Sebia), or with Fat Red 7B. Regardless of staining, both repeatability and reproducibility studies show that all lipoprotein fractions were correctly detected at their respective positions, leading to satisfactory interpretations of lipoproteinograms. Our reproducibility study also confirmed a good stability of the fractions over 6 days (storage at +5 ± 3̊C). In addition, the Fat Red 7B staining leads to a shorter technical time (about 40 min) for the gel drying and staining/destaining phases, which allows us to respond more quickly to certain urgent requests such as chylothorax diagnosis.
    MeSH term(s) Ascitic Fluid/chemistry ; Azo Compounds/chemistry ; Azo Compounds/pharmacology ; Blood Chemical Analysis/methods ; Chemical Fractionation/methods ; Electrophoresis/methods ; Electrophoresis, Agar Gel ; Humans ; Lipoprotein(a)/analysis ; Lipoprotein(a)/blood ; Lipoproteins/analysis ; Lipoproteins/blood ; Reagent Kits, Diagnostic ; Reproducibility of Results ; Staining and Labeling/methods
    Chemical Substances Azo Compounds ; Lipoprotein(a) ; Lipoproteins ; Reagent Kits, Diagnostic ; fat red 7B (6368-72-5)
    Language French
    Publishing date 2020-02-27
    Publishing country France
    Document type Evaluation Study ; Journal Article
    ZDB-ID 418098-7
    ISSN 1950-6112 ; 0003-3898
    ISSN (online) 1950-6112
    ISSN 0003-3898
    DOI 10.1684/abc.2019.1510
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Occurrence of Invasive Pulmonary Fungal Infections in Patients with Severe COVID-19 Admitted to the ICU.

    Fekkar, Arnaud / Lampros, Alexandre / Mayaux, Julien / Poignon, Corentin / Demeret, Sophie / Constantin, Jean-Michel / Marcelin, Anne-Geneviève / Monsel, Antoine / Luyt, Charles-Edouard / Blaize, Marion

    American journal of respiratory and critical care medicine

    2020  Volume 203, Issue 3, Page(s) 307–317

    Abstract: Rationale: ...

    Abstract Rationale:
    MeSH term(s) Aged ; COVID-19/complications ; COVID-19/mortality ; COVID-19/therapy ; Female ; France ; Hospitalization ; Humans ; Invasive Fungal Infections/diagnosis ; Invasive Fungal Infections/epidemiology ; Lung Diseases, Fungal/diagnosis ; Lung Diseases, Fungal/epidemiology ; Male ; Middle Aged ; Respiration, Artificial ; Retrospective Studies ; Risk Factors
    Language English
    Publishing date 2020-08-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1180953-x
    ISSN 1535-4970 ; 0003-0805 ; 1073-449X
    ISSN (online) 1535-4970
    ISSN 0003-0805 ; 1073-449X
    DOI 10.1164/rccm.202009-3400OC
    Database MEDical Literature Analysis and Retrieval System OnLINE

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