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  1. Article: The L.Y.G.H.T. program

    Mitchell, Monique B. / Schuurman, Donna L. / Shapiro, Cheri J. / Sattler, Sebastian / Sorensen, Carl / Martinez, Juliette

    Child and Adolescent Social Work Journal (CASW)

    An evaluation of a peer grief support intervention for youth in foster care

    2022  , Page(s) 1–18

    Abstract: ... the efficacy of a newly developed peer grief support program, the L.Y.G.H.T. program, for youth in foster care ... recommendation of the program, and additional feedback. The findings suggest that the L.Y.G.H.T. program is ...

    Title translation Das L.Y.G.H.T.-Programm: Eine Evaluierung einer Peer-Trauerunterstützungsmaßnahme für Jugendliche in Pflegefamilien
    Abstract Youth in foster care experience high rates of social, emotional, and behavioral challenges that are complicated by trauma and grief. To address these concerns, the current intervention study examines the efficacy of a newly developed peer grief support program, the L.Y.G.H.T. program, for youth in foster care, using an embedded sequential mixed methods design. Forty-two youth residing in three separate foster care group homes ("community sites") in a southeastern state participated in this study. Youth were randomly assigned to either the treatment program or a waitlist control group. Baseline and post-intervention (after 6 weeks) assessments of the Children's Hope Scale, the Inventory of Social Support, the Rosenberg Self-Esteem Scale, and the Strengths and Difficulties Questionnaire-Self Report were administered. After each weekly group, participants completed a survey to assess trauma-informed program environment and facilitators completed a survey to assess fidelity to the intervention. Focus groups (n = 3) were conducted at each of the community sites after the post-intervention assessments were completed. To examine program impact, we evaluated whether increases in well-being (i.e., social support, hopefulness, and self-worth) and decreases in total problem behaviors would be found for participants in the treatment group. Social support, hopefulness, and self-worth increased and total problems remained the same for youth in the treatment program. Large effects were found for program helpfulness, a trauma-informed program environment, and reduction in perceived problems. Focus group feedback explained why youth were initially interested in the program, perceived program benefits, program dislikes, barriers to program participation, recommendation of the program, and additional feedback. The findings suggest that the L.Y.G.H.T. program is an efficacious intervention which addresses loss and grief and enhances the well-being of youth in foster care who are grieving.
    Keywords Evaluation psychosozialer Gesundheitsprogramme ; Foster Care ; Grief ; Intervention ; Mental Health Program Evaluation ; Peers ; Psychische Gesundheit von Jugendlichen ; Social Support ; Soziale Unterstützung ; Trauer ; Versorgung in Pflegefamilien ; Well Being ; Wohlbefinden ; Youth Mental Health
    Language English
    Document type Article
    ZDB-ID 2015019-2
    ISSN 1573-2797 ; 0738-0151
    ISSN (online) 1573-2797
    ISSN 0738-0151
    DOI 10.1007/s10560-022-00843-7
    Database PSYNDEX

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  2. Article ; Online: Structural Studies Reveal Enantiospecific Recognition of a DNA G-Quadruplex by a Ruthenium Polypyridyl Complex.

    McQuaid, Kane / Abell, Holly / Gurung, Sarah P / Allan, David R / Winter, Graeme / Sorensen, Thomas / Cardin, David J / Brazier, John A / Cardin, Christine J / Hall, James P

    Angewandte Chemie (International ed. in English)

    2019  Volume 58, Issue 29, Page(s) 9881–9885

    Abstract: By using X-ray crystallography, we show that the complexes Λ/Δ-[Ru(TAP) ...

    Abstract By using X-ray crystallography, we show that the complexes Λ/Δ-[Ru(TAP)
    Language English
    Publishing date 2019-06-12
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.201814502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The Effects of Sodium Ions on Ligand Binding and Conformational States of G Protein-Coupled Receptors-Insights from Mass Spectrometry.

    Agasid, Mark T / Sørensen, Lars / Urner, Leonhard H / Yan, Jun / Robinson, Carol V

    Journal of the American Chemical Society

    2021  Volume 143, Issue 11, Page(s) 4085–4089

    Abstract: ... ionization emitters, we establish conditions that enable mass spectrometry of two G protein-coupled receptors ...

    Abstract The use of mass spectrometry to investigate proteins is now well established and provides invaluable information for both soluble and membrane protein assemblies. Maintaining transient noncovalent interactions under physiological conditions, however, remains challenging. Here, using nanoscale electrospray ionization emitters, we establish conditions that enable mass spectrometry of two G protein-coupled receptors (GPCR) from buffers containing high concentrations of sodium ions. For the Class A GPCR, the adenosine 2A receptor, we observe ligand-induced changes to sodium binding of the receptor at the level of individual sodium ions. We find that antagonists promote sodium binding while agonists attenuate sodium binding. These findings are in line with high-resolution X-ray crystallography wherein only inactive conformations retain sodium ions in allosteric binding pockets. For the glucagon receptor (a Class B GPCR) we observed enhanced ligand binding in electrospray buffers containing high concentrations of sodium, as opposed to ammonium acetate buffers. A combination of native and -omics mass spectrometry revealed the presence of a lipophilic negative allosteric modulator. These experiments highlight the advantages of implementing native mass spectrometry, from electrospray buffers containing high concentrations of physiologically relevant salts, to inform on allosteric ions or ligands with the potential to define their roles on GPCR function.
    MeSH term(s) Humans ; Ions/chemistry ; Ligands ; Mass Spectrometry ; Models, Molecular ; Receptors, G-Protein-Coupled/chemistry ; Sodium/chemistry
    Chemical Substances Ions ; Ligands ; Receptors, G-Protein-Coupled ; Sodium (9NEZ333N27)
    Language English
    Publishing date 2021-03-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.0c11837
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Retrospective correlation analysis of plasma Immunoglobulin G and clinical performance in CIDP.

    Markvardsen, Lars Kjøbsted / Bruun-Sørensen, Stine / Christiansen, Ingelise / Andersen, Henning

    PeerJ

    2019  Volume 7, Page(s) e6969

    Abstract: ... with immunoglobulin either intravenously (IVIG) or subcutaneously (SCIG). Measurement of plasma immunoglobulin G levels (P-IgG) and ... by the Medical Research Council (MRC) and measurement of plasma immunoglobulin G (P-IgG).: Results: Fifty-five ...

    Abstract Background: Chronic inflammatory demyelinating polyneuropathy (CIDP) can be successfully treated with immunoglobulin either intravenously (IVIG) or subcutaneously (SCIG). Measurement of plasma immunoglobulin G levels (P-IgG) and its correlation to clinical improvement has shown conflicting results. This study aims to clarify whether changes in P-IgG are related to clinical development in patients with CIDP treated with IVIG or SCIG.
    Methods: Patients from five previous studies treated with either IVIG or SCIG with evaluation at baseline and re-evaluation after two or 10/12 weeks, respectively were included. At evaluation and re-evaluation, the following tests were done: combined isokinetic muscle strength (cIKS), grip strength, 9-hole-peg test (9-HPT), 40-meter-walk test (40-MWT), clinical examination of muscle strength score by the Medical Research Council (MRC) and measurement of plasma immunoglobulin G (P-IgG).
    Results: Fifty-five patients were included in the IVIG group and 41 in the SCIG group. There was no correlation between the changes in P-IgG and cIKS in neither the IVIG group (
    Conclusions: In patients with CIDP receiving SCIG or IVIG, changes in P-IgG during treatment did not correlate with changes in muscle strength or other motor performance skills.
    Language English
    Publishing date 2019-05-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.6969
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Discovery of a Recombinant Human Monoclonal Immunoglobulin G Antibody Against α-Latrotoxin From the Mediterranean Black Widow Spider (

    Føns, Sofie / Ledsgaard, Line / Nikolaev, Maxim V / Vassilevski, Alexander A / Sørensen, Christoffer V / Chevalier, Manon K / Fiebig, Michael / Laustsen, Andreas H

    Frontiers in immunology

    2020  Volume 11, Page(s) 587825

    Abstract: Widow spiders are among the few spider species worldwide that can cause serious envenoming in humans. The clinical syndrome resulting ... ...

    Abstract Widow spiders are among the few spider species worldwide that can cause serious envenoming in humans. The clinical syndrome resulting from
    MeSH term(s) Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Monoclonal/pharmacology ; Black Widow Spider/immunology ; Female ; Humans ; Immunoglobulin G/immunology ; Immunoglobulin G/pharmacology ; Male ; Pyramidal Cells/drug effects ; Pyramidal Cells/physiology ; Rats, Wistar ; Spider Venoms/immunology ; Spider Venoms/toxicity
    Chemical Substances Antibodies, Monoclonal ; Immunoglobulin G ; Spider Venoms ; alpha-latrotoxin (65988-34-3)
    Language English
    Publishing date 2020-11-12
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2020.587825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Extended HLA-G haplotypes in patients with age-related macular degeneration.

    Svendsen, Signe Goul / Nilsson, Line Lynge / Djurisic, Snezana / Funck, Tina / Wu, Ching-Lien / Faber, Carsten / Falk, Mads Krüger / Singh, Amardeep / Sørensen, Torben Lykke / Carosella, Edgardo D / LeMaoult, Joël / Hviid, Thomas Vauvert F / Nissen, Mogens Holst

    HLA

    2018  

    Abstract: The study aims to determine if genetic polymorphisms in the human leukocyte antigen (HLA)-G gene ... are associated with age-related macular degeneration (AMD). HLA-G is important for immunological ... regulatory region (URR) and 3'-untranslated region (UTR) of HLA-G have been associated with a number of diseases ...

    Abstract The study aims to determine if genetic polymorphisms in the human leukocyte antigen (HLA)-G gene are associated with age-related macular degeneration (AMD). HLA-G is important for immunological tolerance, and it is also known to have angiogenic effects. Polymorphisms in the 5'-upstream regulatory region (URR) and 3'-untranslated region (UTR) of HLA-G have been associated with a number of diseases, especially with respect to a 14 bp insertion/deletion (ins/del) polymorphism in the 3'UTR. Full gene sequencing was performed on a cohort of 146 AMD patients and 63 healthy controls aged 60 years or older and HLA-G haplotypes were determined. Analyses were performed on a publicly available gene expression dataset from the NCBI GEO database (accession number GSE29801) from which expression data for HLA-G, -C and -A were extracted. Analysis of the GEO dataset showed that both HLA-G and -C was expressed in the back of the eye and that expression was upregulated in the macular area of AMD. No differences were observed between patients and controls when analysing the distribution of haplotypes in the HLA-G promoter, coding region, 3'UTR or the 14 bp ins/del polymorphism of the 3'UTR. The increased expression of HLA-G in the macula of AMD patients indicates a role of HLA-G in the micro environment as part of the AMD pathogenesis. This is supported by the expression of HLA-C, which has previously been shown to play a role in AMD. The HLA-G haplotype distribution did not display any differences between AMD patients and controls. This article is protected by copyright. All rights reserved.
    Language English
    Publishing date 2018-07-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2845111-9
    ISSN 2059-2310 ; 2059-2302
    ISSN (online) 2059-2310
    ISSN 2059-2302
    DOI 10.1111/tan.13340
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Chemogenetic Modulation of G Protein-Coupled Receptor Signalling in Visual Attention Research.

    Jørgensen, Søren H / Fitzpatrick, Ciarán Martin / Gether, Ulrik / Woldbye, David P D / Sørensen, Andreas T

    Basic & clinical pharmacology & toxicology

    2017  Volume 121, Issue 5, Page(s) 373–381

    Abstract: ... manipulation of G protein-coupled receptor (GPCR) signalling, and its broad-ranging usefulness in attention ...

    Abstract Attention is a fundamental cognitive process involved in nearly all aspects of life. Abnormal attentional control is a symptom of many neurological disorders, most notably recognized in ADHD (attention deficit hyperactivity disorder). Although attentional performance and its malfunction has been a major area of investigation, it has proven difficult to accurately associate specific neuronal projections, cell types, neurotransmitter systems and receptors with distinct phenotypes owing to its complexity. In this MiniReview, we present a recently invented technology known as Designer Receptors Exclusively Activated by Designer Drugs (DREADDs). The DREADD technology is an emerging and transformative method that allows selective manipulation of G protein-coupled receptor (GPCR) signalling, and its broad-ranging usefulness in attention research is now beginning to emerge. We first describe the different DREADDs available and explain how unprecedented specificity of neuronal signalling can be achieved using DREADDs. We next discuss various studies performed in animal models of visual attention, where different brain regions and neuronal populations have been probed by DREADDs. We highlight the interplay between the dopamine (DA) and noradrenaline (NA) catecholamine systems in visual attention and explain why DREADD technology can untangle and help us better understand such complex systems in normal and malfunctioning conditions.
    Language English
    Publishing date 2017-11
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 2134679-3
    ISSN 1742-7843 ; 1742-7835
    ISSN (online) 1742-7843
    ISSN 1742-7835
    DOI 10.1111/bcpt.12819
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: The Effects of Sodium Ions on Ligand Binding and Conformational States of G Protein-Coupled Receptors—Insights from Mass Spectrometry

    Agasid, Mark T / Sørensen, Lars / Urner, Leonhard H / Yan, Jun / Robinson, Carol V

    Journal of the American Chemical Society. 2021 Mar. 12, v. 143, no. 11

    2021  

    Abstract: ... ionization emitters, we establish conditions that enable mass spectrometry of two G protein-coupled receptors ...

    Abstract The use of mass spectrometry to investigate proteins is now well established and provides invaluable information for both soluble and membrane protein assemblies. Maintaining transient noncovalent interactions under physiological conditions, however, remains challenging. Here, using nanoscale electrospray ionization emitters, we establish conditions that enable mass spectrometry of two G protein-coupled receptors (GPCR) from buffers containing high concentrations of sodium ions. For the Class A GPCR, the adenosine 2A receptor, we observe ligand-induced changes to sodium binding of the receptor at the level of individual sodium ions. We find that antagonists promote sodium binding while agonists attenuate sodium binding. These findings are in line with high-resolution X-ray crystallography wherein only inactive conformations retain sodium ions in allosteric binding pockets. For the glucagon receptor (a Class B GPCR) we observed enhanced ligand binding in electrospray buffers containing high concentrations of sodium, as opposed to ammonium acetate buffers. A combination of native and -omics mass spectrometry revealed the presence of a lipophilic negative allosteric modulator. These experiments highlight the advantages of implementing native mass spectrometry, from electrospray buffers containing high concentrations of physiologically relevant salts, to inform on allosteric ions or ligands with the potential to define their roles on GPCR function.
    Keywords X-ray diffraction ; adenosine ; ammonium acetate ; electrospray ionization mass spectrometry ; glucagon receptors ; ligands ; lipophilicity ; sodium
    Language English
    Dates of publication 2021-0312
    Size p. 4085-4089.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.0c11837
    Database NAL-Catalogue (AGRICOLA)

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  9. Article ; Online: Retrospective correlation analysis of plasma Immunoglobulin G and clinical performance in CIDP

    Lars Kjøbsted Markvardsen / Stine Bruun-Sørensen / Ingelise Christiansen / Henning Andersen

    PeerJ, Vol 7, p e

    2019  Volume 6969

    Abstract: ... with immunoglobulin either intravenously (IVIG) or subcutaneously (SCIG). Measurement of plasma immunoglobulin G levels (P-IgG) and ... Council (MRC) and measurement of plasma immunoglobulin G (P-IgG). Results Fifty-five patients were ...

    Abstract Background Chronic inflammatory demyelinating polyneuropathy (CIDP) can be successfully treated with immunoglobulin either intravenously (IVIG) or subcutaneously (SCIG). Measurement of plasma immunoglobulin G levels (P-IgG) and its correlation to clinical improvement has shown conflicting results. This study aims to clarify whether changes in P-IgG are related to clinical development in patients with CIDP treated with IVIG or SCIG. Methods Patients from five previous studies treated with either IVIG or SCIG with evaluation at baseline and re-evaluation after two or 10/12 weeks, respectively were included. At evaluation and re-evaluation, the following tests were done: combined isokinetic muscle strength (cIKS), grip strength, 9-hole-peg test (9-HPT), 40-meter-walk test (40-MWT), clinical examination of muscle strength score by the Medical Research Council (MRC) and measurement of plasma immunoglobulin G (P-IgG). Results Fifty-five patients were included in the IVIG group and 41 in the SCIG group. There was no correlation between the changes in P-IgG and cIKS in neither the IVIG group (r = 0.137, p = 0.32) nor the SCIG group (r = − 0.048, p = 0.77). Similarly, no correlations could be demonstrated between P-IgG and grip strength, 9-HPT, 40-MWT or MRC. Conclusions In patients with CIDP receiving SCIG or IVIG, changes in P-IgG during treatment did not correlate with changes in muscle strength or other motor performance skills.
    Keywords Muscle strength ; CIDP ; Plasma immunoglobulin G ; IVIG ; SCIG ; Medicine ; R ; Biology (General) ; QH301-705.5
    Subject code 796
    Language English
    Publishing date 2019-05-01T00:00:00Z
    Publisher PeerJ Inc.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Early BCG-Denmark and Neonatal Mortality Among Infants Weighing <2500 g: A Randomized Controlled Trial.

    Biering-Sørensen, Sofie / Aaby, Peter / Lund, Najaaraq / Monteiro, Ivan / Jensen, Kristoffer Jarlov / Eriksen, Helle Brander / Schaltz-Buchholzer, Frederik / Jørgensen, Anne Sofie Pinstrup / Rodrigues, Amabelia / Fisker, Ane Bærent / Benn, Christine Stabell

    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

    2018  Volume 65, Issue 7, Page(s) 1183–1190

    Abstract: ... given to low-weight (LW) neonates (<2500 g at inclusion) to reduce infant mortality rates, we observed a very ...

    Abstract Background: BCG vaccine may reduce overall mortality by increasing resistance to nontuberculosis infections. In 2 randomized trials in Guinea-Bissau of early BCG-Denmark (Statens Serum Institut) given to low-weight (LW) neonates (<2500 g at inclusion) to reduce infant mortality rates, we observed a very beneficial effect in the neonatal period. We therefore conducted the present trial to test whether early BCG-Denmark reduces neonatal mortality by 45%. We also conducted a meta-analysis of the 3 BCG-Denmark trials.
    Methods: In 2008-2013, we randomized LW neonates to "early BCG-Denmark" (intervention group; n = 2083) or "control" (local policy for LW and no BCG-Denmark; n = 2089) at discharge from the maternity ward or at first contact with the health center. The infants were randomized (1:1) without blinding in blocks of 24. Data was analyzed in Cox hazards models providing mortality rate ratios (MRRs). We had prespecified an analysis censoring follow-up at oral poliovirus vaccine campaigns.
    Results: Early administration of BCG-Denmark was associated with a nonsignificant reduction in neonatal mortality rate (MRR, 0.70; 95% confidence interval [CI], .47-1.04) and a 34% reduction (0.66; .44-1.00) when censoring for oral poliovirus vaccine campaigns. There was no reduction in mortality rate for noninfectious diseases, but a 43% reduction in infectious disease mortality rate (MRR, 0.57; 95% CI, .35-.93). A meta-analysis of 3 BCG trials showed that early BCG-Denmark reduced mortality by 38% (MRR, 0.62; 95% CI, .46-.83) within the neonatal period and 16% (0.84; .71-1.00) by age 12 months.
    Conclusion: Early administration of BCG-Denmark in LW infants is associated with major reductions in mortality rate. It is important that all LW infants receive early BCG in areas with high neonatal mortality rates.
    Clinical trials registration: NCT00625482.
    MeSH term(s) BCG Vaccine/administration & dosage ; BCG Vaccine/immunology ; Communicable Diseases/immunology ; Denmark ; Female ; Guinea-Bissau ; Humans ; Infant ; Infant Mortality ; Infant, Low Birth Weight/immunology ; Infant, Newborn ; Male ; Poliovirus Vaccine, Oral/administration & dosage ; Poliovirus Vaccine, Oral/immunology ; Vaccination/methods
    Chemical Substances BCG Vaccine ; Poliovirus Vaccine, Oral
    Language English
    Publishing date 2018-03-26
    Publishing country United States
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 1099781-7
    ISSN 1537-6591 ; 1058-4838
    ISSN (online) 1537-6591
    ISSN 1058-4838
    DOI 10.1093/cid/cix525
    Database MEDical Literature Analysis and Retrieval System OnLINE

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